Characterization of novel calcium hydroxide‐mediated highly porous chitosan‐calcium scaffolds for potential application in dentin tissue engineering

2020 ◽  
Vol 108 (6) ◽  
pp. 2546-2559 ◽  
Author(s):  
Diana Gabriela Soares ◽  
Ester Alves Ferreira Bordini ◽  
Fernanda Balestrero Cassiano ◽  
Erika Soares Bronze‐Uhle ◽  
Leandro Edgar Pacheco ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Hydroxide ◽  
Potential Application ◽  
Porous Chitosan ◽  
Highly Porous

scholarly journals Fabrication and Characterization of Scaffolds of Poly(ε-caprolactone)/Biosilicate® Biocomposites Prepared by Generative Manufacturing Process

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel Aparecido Lopes Vieira da Cunha ◽  
Paulo Inforçatti Neto ◽  
Kelli Cristina Micocci ◽  
Caroline Faria Bellani ◽  
Heloisa Sobreiro Selistre-de-Araujo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Manufacturing Process ◽  
Potential Application ◽  
Morphological Characterization ◽  
Mechanical Compression ◽  
Compression Tests ◽  
Extrusion Head ◽  
Fabrication And Characterization

Scaffolds of poly(ε-caprolactone) (PCL) and their biocomposites with 0, 1, 3, and 5 wt.% Biosilicate® were fabricated by the generative manufacturing process coupled with a vertical miniscrew extrusion head to application for restoration of bone tissue. Their morphological characterization indicated the designed 0°/90° architecture range of pore sizes and their interconnectivity is feasible for tissue engineering applications. Mechanical compression tests revealed an up to 57% increase in the stiffness of the scaffold structures with the addition of 1 to 5 wt.% Biosilicate® to the biocomposite. No toxicity was detected in the scaffolds tested by in vitro cell viability with MC3T3-E1 preosteoblast cell line. The results highlighted the potential application of scaffolds fabricated with poly(ε-caprolactone)/Biosilicate® to tissue engineering.

scholarly journals Synthesis and Characterization of a New Collagen-Alginate Aerogel for Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Abraham Muñoz-Ruíz ◽  
Diana M. Escobar-García ◽  
Mildred Quintana ◽  
Amaury Pozos-Guillén ◽  
Héctor Flores
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Supercritical Drying ◽  
Drying Process ◽  
Sem Images ◽  
Promote Cell Migration ◽  
Carbon Based Nanomaterials ◽  
Highly Porous ◽  
Alginate Scaffold

Scaffolds have been used as extracellular matrix analogs to promote cell migration, cell attachment, and cell proliferation. The use of aerogels and carbon-based nanomaterials has recently been proposed for tissue engineering due to their properties. The aim of this study is to develop a highly porous collagen-alginate(-graphene oxide) aerogel-based scaffold. The GO synthesis was performed by Hummers method; a collagen-alginate and collagen-alginate-GO hydrogel were synthetized; then, they were treated by a supercritical drying process. The aerogels obtained were evaluated by SEM and FTIR. Osteoblasts were seeded over the scaffolds and evaluated by SEM. According to the characterization, the aerogels showed a highly porous interconnected network covered by a nonporous external wall. According to the FTIR, the chemical functional groups of collagen and GO were maintained after the supercritical process. The SEM images after cell culture showed that a collagen-alginate scaffold promotes cell attachment and proliferation. The alginate-collagen aerogel-based scaffold could be a platform for tissue engineering since it shows adequate properties. Further studies are needed to determine the cell interactions with GO.

Characterization of a Novel Polymeric Scaffold for Potential Application in Tendon/Ligament Tissue Engineering

2006 ◽  
Vol 0 (0) ◽  
pp. 060118075515005 ◽  
Author(s):  
S. Sahoo ◽  
H. Ouyang ◽  
James C.-H. Goh ◽  
T.E. Tay ◽  
S.L. Toh
Keyword(s):  
Tissue Engineering ◽  
Potential Application ◽  
Polymeric Scaffold ◽  
Ligament Tissue Engineering

Preparation and Characterization of Layer Structured Porous Chitosan Scaffold for Tissue Engineering

2012 ◽  
Vol 198-199 ◽  
pp. 179-182
Author(s):  
Guo Jun Song ◽  
Xue Jun Wang ◽  
Tao Lou ◽  
Li Yong Lv
Keyword(s):  
Tissue Engineering ◽  
Acetic Acid ◽  
Phase Separation ◽  
Layer Structure ◽  
Compressive Modulus ◽  
High Porosity ◽  
Chitosan Scaffold ◽  
Chitosan Concentration ◽  
Porous Chitosan

In this study, layer structured porous chitosan scaffold was successfully fabricated using thermal induced phase separation method. The scaffold had a layer structure with interconnective pores (50- 300 μm) and high porosity (>90%) using citric or acetic acid as the solvent. However, the results of compressive modulus of the scaffold showed that acetic acid was a better choice, and the compressive modulus of scaffold increased with chitosan concentration in acetic acid. The scaffold is very promising for tissue engineering.

scholarly journals Preparation and characterization of Copper Chitosan Nanocomposites with Antibacterial Activity for Applications in Tissue Engineering

2017 ◽  
Author(s):  
◽  
K. Maldonado-Lara
Keyword(s):  
Tissue Engineering ◽  
Antibacterial Activity ◽  
Potential Application ◽  
Antibacterial Properties ◽  
Hydroxyl Groups ◽  
Liquid Media ◽  
Solution Blending ◽  
Nanoparticles Dispersion ◽  
Bacterial Penetration

The Present work describes the preparation of nanocomposites based on chitosan (QS)/copper nanoparticles (nCu) with antibacterial properties and potential application in tissue engineering. For this purpose, nanocomposites were prepared by solution blending with ultrasound assisted, aiming to increase the nanoparticles dispersion in the biopolymer. FTIR analyses demonstrates that nCu supported in QS increase their interaction of nanoparticles with amine/hydroxyl groups of QS molecule. UV-Vis analyses demonstrates that QS/nCu nanocomposites have an absorption signal associated with the presence of nanoparticles and the possible Cu2+ ions release in liquid media. AFM analyses shown that hydrated QS form a mesh with micro pores, improving the bacterial penetration and the direct contact with nCu. This behavior was corroborated by antibacterial assays, where QS/nCu nanocomposites shown an antibacterial activity higher than 90% between 90-180 minutes of interaction. Our results suggest that is possible to obtain combined antibacterial/biocompatible nanomaterials with potential application in tissue engineering.

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