scholarly journals Keeping a Completely Autotrophic Nitrogen Removal over Nitrite System Effective in Treating Low Ammonium Wastewater by Adopting an Alternative Low and High Ammonium Influent Regime

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Qinglong Chang ◽  
Weigang Wang ◽  
Jie Chen ◽  
Yayi Wang
Keyword(s):  
High Throughput ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Operating Mode ◽  
Ammonium Concentration ◽  
Nitrite Oxidation ◽  
Time Control ◽  
Nitrite Oxidizing Bacteria ◽  
Excessive Proliferation ◽  
Autotrophic Nitrogen Removal

An alternative low and high ammonium influent regime was proposed and adopted to keep a completely autotrophic nitrogen removal over nitrite (CANON) effective when treating low ammonium wastewater. Results show that, by cyclic operating at an alternative low and high ammonium concentration for 10 days and 28 days, the CANON system could effectively treat low ammonium wastewater. Excessive proliferation of nitrite oxidizing bacteria (NOB) under low ammonium environment was still the challenge for the stable CANON operation; but with 28 days of a high ammonium treatment combined with a sludge retention time control, the NOB overproliferated in the low ammonium operational period could be under control. Specifically, when the nitrite oxidation rate reached 8 g N/m3/h, the CANON system should enter the high ammonium influent operating mode. 16S rDNA high-throughput sequencing results show that the appropriate sludge discharging provided an environment favoring Candidatus Jettenia.

scholarly journals Nitrification mainly driven by ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an anammox-inoculated wastewater treatment system

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Lu ◽  
Yiguo Hong ◽  
Ying Wei ◽  
Ji-Dong Gu ◽  
Jiapeng Wu ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Ammonia Oxidation ◽  
Ammonia Oxidizing Bacteria ◽  
Ammonium Oxidation ◽  
Strong Activity ◽  
Nitrite Oxidizing Bacteria

AbstractAnaerobic ammonium oxidation (anammox) process has been acknowledged as an environmentally friendly and time-saving technique capable of achieving efficient nitrogen removal. However, the community of nitrification process in anammox-inoculated wastewater treatment plants (WWTPs) has not been elucidated. In this study, ammonia oxidation (AO) and nitrite oxidation (NO) rates were analyzed with the incubation of activated sludge from Xinfeng WWTPs (Taiwan, China), and the community composition of nitrification communities were investigated by high-throughput sequencing. Results showed that both AO and NO had strong activity in the activated sludge. The average rates of AO and NO in sample A were 6.51 µmol L−1 h−1 and 6.52 µmol L−1 h−1, respectively, while the rates in sample B were 14.48 µmol L−1 h−1 and 14.59 µmol L−1 h−1, respectively. The abundance of the nitrite-oxidizing bacteria (NOB) Nitrospira was 0.89–4.95 × 1011 copies/g in both samples A and B, the abundance of ammonia-oxidizing bacteria (AOB) was 1.01–9.74 × 109 copies/g. In contrast, the abundance of ammonia-oxidizing archaea (AOA) was much lower than AOB, only with 1.28–1.53 × 105 copies/g in samples A and B. The AOA community was dominated by Nitrosotenuis, Nitrosocosmicus, and Nitrososphaera, while the AOB community mainly consisted of Nitrosomonas and Nitrosococcus. The dominant species of Nitrospira were Candidatus Nitrospira defluvii, Candidatus Nitrospira Ecomare2 and Nitrospira inopinata. In summary, the strong nitrification activity was mainly catalyzed by AOB and Nitrospira, maintaining high efficiency in nitrogen removal in the anammox-inoculated WWTPs by providing the substrates required for denitrification and anammox processes.

Completely Autotrophic Nitrogen-Removal for Treatment of High Ammonia Leachate

2010 ◽  
Vol 113-116 ◽  
pp. 662-665
Author(s):  
Wen De Tian ◽  
Kyoung Jin An ◽  
Zhi Wei Li
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Optimal Operation ◽  
Ammonium Concentration ◽  
Operation Condition ◽  
Partial Nitritation ◽  
Ammonium Removal ◽  
High Ammonia ◽  
Anammox Process ◽  
Autotrophic Nitrogen Removal

This study focused on the feasibility of autotrophic nitrogen removal to treat high ammonia leachate, using combined partial Nitritation and Anammox process. In partial nitritation reactor, the optimal operation condition was found with influent ammonium concentration of 1200 mg/L, DO about 3 mg/L, HRT 3 days and temperature about 31°C at the ratio of NO2-N / NH4-N effluent kept at 1.1, which is a prerequisite for the application of Anammox. In Anammox reactor, more than 85% ammonium is removed at HRT 8 days, temperature 28±1°C, and pH 8. The removal rate of nitrogen and COD in combined partial Nitritation and Anammox process are 90% and 74%, respectively. Thus, a combined process of partial nitritation and a subsequent Anammox could be an alternative solution for ammonium removal for leachate.

scholarly journals Model-based evaluation of the contribution of partial denitrification (PD) on the one-stage autotrophic nitrogen removal process

2021 ◽  
Author(s):  
Chi Zhang ◽  
Lianze Yu ◽  
Miao Zhang ◽  
Jun Wu
Keyword(s):  
Nitrogen Removal ◽  
Oxygen Demand ◽  
Anammox Bacteria ◽  
Removal Process ◽  
One Stage ◽  
Biofilm Model ◽  
Nitrite Oxidizing Bacteria ◽  
The One ◽  
Steady State Simulation ◽  
Autotrophic Nitrogen Removal

Abstract The nitrate produced by the one-stage partial nitritation-anammox (PN/A) process can be removed through partial denitrification (PD) by adding carbon source. In this study, a 1D multi-population biofilm model was developed to evaluate the contribution of partial denitrification on the one-stage autotrophic nitrogen removal process at influent NH4+ = 100 mg N/L. The dynamic simulation that was carried out to investigate the effect of nitrite-oxidizing bacteria (NOB) revealed that PD contributed to the reactor to obtain total nitrogen removal efficiency (TNR) of above 90% and the effluent nitrate was significantly decreased with the absence of NOB. However, PD decreased TNR of the one-stage PN/A process with the presence of NOB. Increased influent chemical oxygen demand (COD) widened the dissolved oxygen (DO) range required for high TNR whether NOB were present or not. The steady-state simulation results showed that NOB were always absent in the granules at high DO and COD levels and the optimum DO > 0.5 mg/L when influent COD was over 50 mg/L. Besides, higher influent COD/NH4+ (C/N) and larger granule diameter (diameter > 1600 µm) were contributed to widening the range of DO required for high TNR. The nitrogen removal contribution of anammox bacteria (AMX) was significantly higher than denitrification in the reactor.

scholarly journals Impact of salinity on the performance and microbial community of anaerobic ammonia oxidation (Anammox) using 16S rRNA High-throughput Sequencing technology

2017 ◽  
Vol 19 (3) ◽  
pp. 377-388 ◽  
Keyword(s):  
16S Rrna ◽  
High Throughput ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Community Dynamics ◽  
Removal Rate ◽  
System Response ◽  
Anammox Bacteria ◽  
Term Operation ◽  
Anammox Activity

Salinity is a key environmental factor for the successful application of anammox technology in wastewater treatment, because it impacts the activity and the community structure of anammox bacteria. In this study, the changes in activity and population shifts of an anammox system response to the elevated salt stress (0, 5, 10, 20, 30 and 40 g NaCl/L) were studied. The results show that the anammox reactor performed effectively even at 30 g NaCl/L salinity after an appropriate acclimatization. The nitrogen removal rate maintained at 0.28 g N L-1d-1 with the nitrogen removal efficiency of 76%, though the high environmental salinity might inhibit the anammox growth in the long-term operation. 16S rRNA high-throughput sequencing results revealed that Ca. Brocadia, Ca. Jettenia and Ca. Kuenenia were the dominant anammox bacteria at all salinities. Ca. Brocadia and Ca. Jettenia were quite sensitive to salinity, and 5 g NaCl/L dosing could cause a sharp decline in their abundance. Nevertheless, these three anammox genus finally survived in the system with a steady specific anammox activity of 0.13 g N g VSS-1d-1. Specially, a novel cluster, Brocadiaceae_unclassified, which possibly belongs to anammox bacteria, became the dominant genus at the salinity over 20 g NaCl/L and likely contributed partially to the nitrogen removals. Our findings elucidated the inherent link between community dynamics and anammox system performance and stability under salty environment, and proved that anammox technologies can be an effective technology for treatment of saline ammonia-rich wastewater.

Numerical modeling of nitrogen removal processes in biofilters with simultaneous nitritation and anammox

2013 ◽  
Vol 67 (3) ◽  
pp. 549-556
Author(s):  
Shun Shi ◽  
Wendong Tao
Keyword(s):  
Nitrogen Removal ◽  
Ammonium Concentration ◽  
Ammonia Oxidizing Bacteria ◽  
Ammonium Oxidation ◽  
Nitrite Oxidation ◽  
Ammonium Removal ◽  
Natural Treatment Systems ◽  
Natural Treatment ◽  
Removal Processes ◽  
Startup Period

This study developed a simple numerical model for nitrogen removal in biofilters, which was designed to enhance simultaneous nitritation and anaerobic ammonium oxidation (anammox). It is the first attempt to simulate anammox together with two-step nitrification in natural treatment systems, which may have different kinetic parameters and temperature effects from conventional bioreactors. Prediction accuracy was improved by adjusting kinetic coefficients over the startup period of the biofilters. The maximum rates of nitritation and nitrite oxidation increased linearly over time during the startup period. Simulations confirmed successful enhancement of simultaneous nitritation and anammox (SNA) in the biofilters, with anammox contributing 35% of ammonium removal. Effluent ammonium concentration was affected by influent ammonium concentration and the maximum nitritation rate, and was insensitive to the maximum nitrite oxidation rate and anammox substrate factor. Ammonium removal via SNA was likely limited by biomass of aerobic ammonia oxidizing bacteria in the biofilters. The developed model is a promising tool for studying the dynamics of nitrogen removal processes including SNA in natural treatment systems.

scholarly journals Characteristics of Biological Nitrogen Removal in a Multiple Anoxic and Aerobic Biological Nutrient Removal Process

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Huoqing Wang ◽  
Yuntao Guan ◽  
Li Li ◽  
Guangxue Wu
Keyword(s):  
Organic Carbon ◽  
Nitrogen Removal ◽  
Operating Mode ◽  
Biological Nutrient Removal ◽  
Biological Nitrogen Removal ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Term Operation ◽  
Nitrite Oxidizing Bacteria ◽  
Biological Nitrogen

Two sequencing batch reactors, one with the conventional anoxic and aerobic (AO) process and the other with the multiple AO process, were operated to examine characteristics of biological nitrogen removal, especially of the multiple AO process. The long-term operation showed that the total nitrogen removal percentage of the multiple AO reactor was 38.7% higher than that of the AO reactor. In the multiple AO reactor, at the initial SBR cycle stage, due to the occurrence of simultaneous nitrification and denitrification, no nitrite and/or nitrate were accumulated. In the multiple AO reactor, activities of nitrite oxidizing bacteria were inhibited due to the multiple AO operating mode applied, resulting in the partial nitrification. Denitrifiers in the multiple AO reactor mainly utilized internal organic carbon for denitrification, and their activities were lower than those of denitrifiers in the AO reactor utilizing external organic carbon.

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