New nitrogen removal pathways in a full-scale hybrid constructed wetland proposed from high-throughput sequencing and isotopic tracing results

2016 ◽  
Vol 97 ◽  
pp. 434-443 ◽  
Author(s):  
Jun Zhai ◽  
Md. Hasibur Rahaman ◽  
Xing Chen ◽  
Haiwen Xiao ◽  
Kaisen Liao ◽  
...  
Keyword(s):  
High Throughput ◽  
Nitrogen Removal ◽  
Constructed Wetland ◽  
High Throughput Sequencing ◽  
Full Scale ◽  
Isotopic Tracing

scholarly journals Keeping a Completely Autotrophic Nitrogen Removal over Nitrite System Effective in Treating Low Ammonium Wastewater by Adopting an Alternative Low and High Ammonium Influent Regime

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Qinglong Chang ◽  
Weigang Wang ◽  
Jie Chen ◽  
Yayi Wang
Keyword(s):  
High Throughput ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Operating Mode ◽  
Ammonium Concentration ◽  
Nitrite Oxidation ◽  
Time Control ◽  
Nitrite Oxidizing Bacteria ◽  
Excessive Proliferation ◽  
Autotrophic Nitrogen Removal

An alternative low and high ammonium influent regime was proposed and adopted to keep a completely autotrophic nitrogen removal over nitrite (CANON) effective when treating low ammonium wastewater. Results show that, by cyclic operating at an alternative low and high ammonium concentration for 10 days and 28 days, the CANON system could effectively treat low ammonium wastewater. Excessive proliferation of nitrite oxidizing bacteria (NOB) under low ammonium environment was still the challenge for the stable CANON operation; but with 28 days of a high ammonium treatment combined with a sludge retention time control, the NOB overproliferated in the low ammonium operational period could be under control. Specifically, when the nitrite oxidation rate reached 8 g N/m3/h, the CANON system should enter the high ammonium influent operating mode. 16S rDNA high-throughput sequencing results show that the appropriate sludge discharging provided an environment favoring Candidatus Jettenia.

scholarly journals Impacting Microbial Communities and Absorbing Pollutants by Canna Indica and Cyperus Alternifolius in a Full-Scale Constructed Wetland System

2019 ◽  
Vol 16 (5) ◽  
pp. 802 ◽  
Author(s):  
Yinghai Wu ◽  
Tao He ◽  
Chen Chen ◽  
Xiaohang Fang ◽  
Dongyang Wei ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Plant Species ◽  
Nitrogen Removal ◽  
Constructed Wetland ◽  
Full Scale ◽  
Canna Indica ◽  
Subsurface Flow Constructed Wetland ◽  
Wetland System ◽  
Cyperus Alternifolius ◽  
Rhizosphere Sediment

Wetland plants that cover the wetlands play an important role in reducing pollutants. The aim of this study was to investigate the effect of two plant species on microbial communities and nitrogen-removal genes and to evaluate the contributions of absorbing pollutants by Canna indica (CI) and Cyperus alternifolius (CA) to the removal performance in both a vertical subsurface flow constructed wetland and a horizontal subsurface flow constructed wetland, which were part of a full-scale hybrid constructed wetland system. The microbial assemblages were determined using 16S rRNA high-throughput sequencing. Results showed that the presence of CI and CA positively affected microbial abundance and community in general and which was positive for the total bacteria and ammonia nitrogen removal in the CWs. The higher abundance of Nitrospirae appeared in the non-rhizosphere sediment (NRS) than that in the rhizosphere sediment (RS). More denitrification genes were found in NRS than in RS. The copy numbers of narG, nirS and nosZ genes for CA were higher than those for CI. Wetland plant species can significantly (P < 0.05) affect the distribution of microbial communities in RS. Plant selection is important to promote the development of microbial communities with a more active and diverse catabolic capability and the contribution of plant absorption to the overall removal rate of wetland system can be neglected.

scholarly journals Impact of salinity on the performance and microbial community of anaerobic ammonia oxidation (Anammox) using 16S rRNA High-throughput Sequencing technology

2017 ◽  
Vol 19 (3) ◽  
pp. 377-388 ◽  
Keyword(s):  
16S Rrna ◽  
High Throughput ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Community Dynamics ◽  
Removal Rate ◽  
System Response ◽  
Anammox Bacteria ◽  
Term Operation ◽  
Anammox Activity

Salinity is a key environmental factor for the successful application of anammox technology in wastewater treatment, because it impacts the activity and the community structure of anammox bacteria. In this study, the changes in activity and population shifts of an anammox system response to the elevated salt stress (0, 5, 10, 20, 30 and 40 g NaCl/L) were studied. The results show that the anammox reactor performed effectively even at 30 g NaCl/L salinity after an appropriate acclimatization. The nitrogen removal rate maintained at 0.28 g N L-1d-1 with the nitrogen removal efficiency of 76%, though the high environmental salinity might inhibit the anammox growth in the long-term operation. 16S rRNA high-throughput sequencing results revealed that Ca. Brocadia, Ca. Jettenia and Ca. Kuenenia were the dominant anammox bacteria at all salinities. Ca. Brocadia and Ca. Jettenia were quite sensitive to salinity, and 5 g NaCl/L dosing could cause a sharp decline in their abundance. Nevertheless, these three anammox genus finally survived in the system with a steady specific anammox activity of 0.13 g N g VSS-1d-1. Specially, a novel cluster, Brocadiaceae_unclassified, which possibly belongs to anammox bacteria, became the dominant genus at the salinity over 20 g NaCl/L and likely contributed partially to the nitrogen removals. Our findings elucidated the inherent link between community dynamics and anammox system performance and stability under salty environment, and proved that anammox technologies can be an effective technology for treatment of saline ammonia-rich wastewater.

Nitrogen removal from agricultural runoff by full-scale constructed wetland in China

Hydrobiologia ◽  
2008 ◽  
Vol 621 (1) ◽  
pp. 115-126 ◽  
Author(s):  
Shaoyong Lu ◽  
Pengyi Zhang ◽  
Xiangcan Jin ◽  
Changsheng Xiang ◽  
Meng Gui ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Constructed Wetland ◽  
Agricultural Runoff ◽  
Full Scale
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