scholarly journals Antimicrobial Activity ofPiper marginatumJacq andIlex guayusaLoes on Microorganisms Associated with Periodontal Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Fredy Gamboa ◽  
Camila-Cristina Muñoz ◽  
Gloria Numpaque ◽  
Luis Gonzalo Sequeda-Castañeda ◽  
Sandra Janeth Gutierrez ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Antimicrobial Activity ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Ethanol Extract ◽  
Fusobacterium Nucleatum ◽  
Diffusion Method ◽  
Prevotella Intermedia ◽  
Qualitative And Quantitative ◽  
Ethanol Extracts

Background. Chronic periodontitis is a multifactorial infectious disease, where multiple bacteria, such asPorphyromonas gingivalis,Prevotella intermedia, andFusobacterium nucleatumare implicated. The main purpose of researching natural products is to find substances or compounds with antimicrobial activity.Aim. The objective of this work was to determine antimicrobial activity from extracts and obtained fractions fromPiper marginatumJacq andIlex guayusaLoes onP. gingivalisATCC 33277,F. nucleatumATCC 25586, andP. intermediaATCC 25611.Methods. Total ethanol extracts were obtained from both plants. Fractions were obtained from total ethanol extracts with amberlite as a stationary phase employing hexane, acetone, and ethanol-water as solvents. Qualitative and quantitative phytochemical characterization was performed on total ethanol extracts from both plants. Antimicrobial activity from total ethanol extracts and fractions from both plants were evaluated onP. gingivalisATCC 33277,F. nucleatumATCC 25586, andP. intermediaATCC by the well diffusion method with Wilkins–Chalgren agar.Results.Piper marginatumJacq total ethanol extract presented antimicrobial activity against all three bacteria, whereasIlex guayusaLoes was only efficient againstP. gingivalisATCC 33277 andP. intermediaATCC 25611, with inhibition halos from 9.3 to 30 mm.Ilex guayusaLoes obtained fractions presented antimicrobial activity against all three microorganisms evaluated, with inhibition halos ranging from 9.7 to 18.7 mm. In regards toPiper marginatumJacq fractions, inhibition halos were between 8.3 and 19 mm, against all three microorganisms evaluated; only hexane fraction did not present antimicrobial activity againstF. nucleatumATCC 25586.Conclusion.Piper marginatumJacq andIlex guayusaLoes total ethanol extracts and fractions presented outstanding antimicrobial activity againstP. gingivalisATCC 33277,P. intermediaATCC 25611, andF. nucleatumATCC 25586.

scholarly journals Preliminary Phytochemical Analysis of Berberis goudotii Triana & Planch. ex Wedd. (Berberidaceae) with Anticariogenic and Antiperiodontal Activities

2019 ◽  
Vol 87 (1) ◽  
pp. 2 ◽  
Author(s):  
Luis Sequeda-Castañeda ◽  
Camila Muñoz-Realpe ◽  
Crispín Celis-Zambrano ◽  
Sandra Gutiérrez-Prieto ◽  
Pilar Luengas-Caicedo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Porphyromonas Gingivalis ◽  
Fusobacterium Nucleatum ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Prevotella Intermedia ◽  
Streptococcus Sobrinus ◽  
Total Extract ◽  
Liquid Fractionation

Berberis goudotii is an endemic Colombian plant found in the paramo ecosystem. It has been used in food preparation and as a medicinal plant for diverse treatments. Additionally, it is used as a mouthwash to strengthen the gums and combat throat irritations and periodontitis. The present research evaluated Berberis goudotii aerial parts extract and fractions antimicrobial activities. Ultrasonic-assisted extraction was used to attain total ethanol-water extract. Solid-liquid fractionation was used to obtain hexane fraction. The residue was dispersed in water and liquid-liquid fractionation was carried-out to acquire dichloromethane, butanol and water fractions. Preliminary phytochemical analysis was performed on total extract and phenol, polyphenol, flavonoid, and proanthocyanidin, while tannin content was quantified. Antimicrobial activity assessment was performed by agar diffusion method using disks and wells employing Ceftazidime as a positive control against Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei, Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum. Antimicrobial activity was determined as relative percentage inhibition (RPI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phenols (92.5 ± 7.7 mg GA/10 g), polyphenols (87.7 ± 8.1 mg PG/10 g) and tannins (44.1 ± 4.3 mg PG/10 g) were among the highest secondary metabolites observed. Total extract presented an MBC of 1.0 µg/µL against cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus) and 0.12 µg/µL against bacteria associated with periodontal disease (Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum). Butanol and hexane fractions showed antiperiodontal activity with MBC of 0.12 and 1.0 µg/µL, respectively. In conclusion, Berberis goudotii total extract demonstrated antimicrobial activity against cariogenic and periodontal microorganisms, on the other hand, hexane and butanol fractions displayed antiperiodontal activity.

scholarly journals Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles

2017 ◽  
Vol 15 (2) ◽  
pp. 96-99
Author(s):  
SALEM MOHAMED EDRAH
Keyword(s):  
Antimicrobial Activity ◽  
Short Communication ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Portulaca Oleracea ◽  
Diffusion Method ◽  
Ethanolic Extracts ◽  
Communication Evaluation ◽  
Ethanol Extracts ◽  
Phytochemical Profiles

Edrah SM. 2017. Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles. Biofarmasi J Nat Prod Biochem 15: 96-99. The ethanol extracts of leaves of Alchemilla vulgaris and Portulaca oleracea were studied for antimicrobial activity at 10 mg/mL concentrations by using disc diffusion method on two gram-positive bacteria: Staphylococcus aureus and Staphylococcus epidermidis; three gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and one fungi: Candida albicans were used in the study. After incubation for 24 hrs, the zone of inhibition was compared with standard antibiotics Gentamycin (10 μg/disc) which was used as positive control. From the dose-dependent study, it was concluded that the ethanol extract of A. vulgaris was more potential than leave extract of P. oleracea. Almost all of the chemical ingredients present in both ethanol extracts such as tannins, flavonoids, phenols which may be responsible for the antimicrobial activity.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals Perbandingan Aktivitas Antimikroba Ekstrak Etanol dari Tiga Spesies Ganoderma Asal Pulau Lombok

2022 ◽  
Vol 7 (2) ◽  
pp. 160-172
Author(s):  
Faturrahman Faturrahman ◽  
Sukiman Sukiman ◽  
Bambang Fajar Suryadi ◽  
Sarkono Sarkono ◽  
Ernin Hidayati
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Cryptococcus Neoformans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Sample Collection ◽  
Gram Positive ◽  
Gram Negative ◽  
Microbial Infections ◽  
Ethanol Extracts

The use of antibiotics is one of the most important ways to deal with the spread and treatment of pathogenic microbial infections. The search for new antibiotic sources continues to be carried out to anticipate the emergence of microbial resistance. One of the natural resources that has the potential as an antimicrobial source is a member of the macrofungi of the Genus Ganoderma. The purpose of this study was to evaluate the antimicrobial performance of the ethanol extracts of Ganoderma  lucidum, G. applanatum dan Ganorderma sp. against fungi (Candida albicans dan Cryptococcus neoformans),  gram positive bacteria (Bacillus cereus dan Staphylococcus aureus) and gram negative bacteria (Escherichia coli dan Shigella sp.). Macrofungi samples were taken from the forest area of Nature Tourism Park (TWA) Gunung Tunak, TWA Kerandangan, TWA Suranadi, TWA Nuraksa Sesaot, TWA Lemor and Pusuk forest. The stages of the method performed are sample collection, sample preparation, extraction, and testing of antimicrobial activity using the well difusion method. The ethanol extract concentrations for testing were 20%, 40%, 60% and 80%. The results showed that the three Ganoderma species had anti-fungal and antibacterial activity and that different levels of concentration had an effect on inhibition. The size of the inhibition zone is directly proportional to the higher the extract concentration. The antimicrobial activity of the ethanol extract of G. lucidum was higher when compared to G. applanatum and Ganoderma sp. both against fungi (Candida albicans and Cryptococcus neoformans) as well as against gram-positive and gram-negative test bacteria. In addition, G. applanatum showed very weak inhibition against both groups of tested bacteria.  Key words: candidiasis; cryptococcosis; comorbid infections; macerations; pathogens

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