scholarly journals Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles

2017 ◽  
Vol 15 (2) ◽  
pp. 96-99
Author(s):  
SALEM MOHAMED EDRAH
Keyword(s):  
Antimicrobial Activity ◽  
Short Communication ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Portulaca Oleracea ◽  
Diffusion Method ◽  
Ethanolic Extracts ◽  
Communication Evaluation ◽  
Ethanol Extracts ◽  
Phytochemical Profiles

Edrah SM. 2017. Short Communication: Evaluation of antimicrobial activities of Alchemilla vulgaris and Portulaca oleracea ethanolic extracts and correlation with their phytochemical profiles. Biofarmasi J Nat Prod Biochem 15: 96-99. The ethanol extracts of leaves of Alchemilla vulgaris and Portulaca oleracea were studied for antimicrobial activity at 10 mg/mL concentrations by using disc diffusion method on two gram-positive bacteria: Staphylococcus aureus and Staphylococcus epidermidis; three gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and one fungi: Candida albicans were used in the study. After incubation for 24 hrs, the zone of inhibition was compared with standard antibiotics Gentamycin (10 μg/disc) which was used as positive control. From the dose-dependent study, it was concluded that the ethanol extract of A. vulgaris was more potential than leave extract of P. oleracea. Almost all of the chemical ingredients present in both ethanol extracts such as tannins, flavonoids, phenols which may be responsible for the antimicrobial activity.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals Antimicrobial Activity ofPiper marginatumJacq andIlex guayusaLoes on Microorganisms Associated with Periodontal Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Fredy Gamboa ◽  
Camila-Cristina Muñoz ◽  
Gloria Numpaque ◽  
Luis Gonzalo Sequeda-Castañeda ◽  
Sandra Janeth Gutierrez ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Antimicrobial Activity ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Ethanol Extract ◽  
Fusobacterium Nucleatum ◽  
Diffusion Method ◽  
Prevotella Intermedia ◽  
Qualitative And Quantitative ◽  
Ethanol Extracts

Background. Chronic periodontitis is a multifactorial infectious disease, where multiple bacteria, such asPorphyromonas gingivalis,Prevotella intermedia, andFusobacterium nucleatumare implicated. The main purpose of researching natural products is to find substances or compounds with antimicrobial activity.Aim. The objective of this work was to determine antimicrobial activity from extracts and obtained fractions fromPiper marginatumJacq andIlex guayusaLoes onP. gingivalisATCC 33277,F. nucleatumATCC 25586, andP. intermediaATCC 25611.Methods. Total ethanol extracts were obtained from both plants. Fractions were obtained from total ethanol extracts with amberlite as a stationary phase employing hexane, acetone, and ethanol-water as solvents. Qualitative and quantitative phytochemical characterization was performed on total ethanol extracts from both plants. Antimicrobial activity from total ethanol extracts and fractions from both plants were evaluated onP. gingivalisATCC 33277,F. nucleatumATCC 25586, andP. intermediaATCC by the well diffusion method with Wilkins–Chalgren agar.Results.Piper marginatumJacq total ethanol extract presented antimicrobial activity against all three bacteria, whereasIlex guayusaLoes was only efficient againstP. gingivalisATCC 33277 andP. intermediaATCC 25611, with inhibition halos from 9.3 to 30 mm.Ilex guayusaLoes obtained fractions presented antimicrobial activity against all three microorganisms evaluated, with inhibition halos ranging from 9.7 to 18.7 mm. In regards toPiper marginatumJacq fractions, inhibition halos were between 8.3 and 19 mm, against all three microorganisms evaluated; only hexane fraction did not present antimicrobial activity againstF. nucleatumATCC 25586.Conclusion.Piper marginatumJacq andIlex guayusaLoes total ethanol extracts and fractions presented outstanding antimicrobial activity againstP. gingivalisATCC 33277,P. intermediaATCC 25611, andF. nucleatumATCC 25586.

scholarly journals Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde

2021 ◽  
Vol 9 (10) ◽  
pp. 1915-1920
Author(s):  
Şükrü Canpolat ◽  
Ilgaz Akata ◽  
Yasemin İşlek ◽  
Elif Canpolat ◽  
Cemil İşlek
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Morchella Esculenta ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.

scholarly journals Antimicrobial Activity of Some Medicinal Plants from East and Central Part of Nepal

2014 ◽  
Vol 2 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Bimala Subba ◽  
Prakash Basnet
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts ◽  
Inhibitory Activities

To evaluate the antimicrobial activities of extract of eleven plants were examined against four common bacterials. The ethanolic extracts of various plants such as  Cissus repens, Hedyotis scandens, Jatropha curcas, Morus alba, Inula cappa, Equisetum ramosissimum, Osyris wightiana, Alternantheria sessilis and Hibiscus lampas  investigated individually for antimicrobial activity by disc diffusion method. These were investigated against selected species of Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Klebsiella pneumoniae to find the inhibitory activities of the microbes. The ethanolic extract of C. repens showed considerably high activity against P. vulgaris, E. coli and S. aureus than other extracts.DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9697Int J Appl Sci Biotechnol, Vol. 2(1): 88-92

scholarly journals Screening and characterization of potential bioactive compounds from Selaginella wightii

2020 ◽  
Author(s):  
Abheepsa Mishra
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Diethyl Ether ◽  
Fungal Infections ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Ether Extract ◽  
Diethyl Ether Extract ◽  
Dpph Method

A chemical investigation of the whole plant of Selaginella wightii was undertaken and the extracts of Selaginella wightii were screened for antioxidant and antimicrobial activities . The antioxidant activity by DPPH method was higher in ethanol extract and diethyl ether extract. In β-carotene-Linoleic acid assay, ethanol extract and diethyl ether extract showed good inhibition zone exhibiting significant antioxidant activity. The various extracts were then examined for antimicrobial activity by using the standard well-diffusion method. The diethyl ether extracts, ethanol, and aqueous extracts showed higher levels of antimicrobial activity than the other extracts. All the extracts also showed good anticandidal activity. The extracts were found to be rich in flavonoids and three compounds were identified. Our study indicates the potential use of Selaginella wightii extracts for treating antimicrobial or fungal infections.

scholarly journals Antibacterial and antifungal activities of the ethanol extract of some medicinal plants

2020 ◽  
Vol 3 (4) ◽  
pp. 43-51
Author(s):  
Ngoc Nguyen Nhu ◽  
Minh Vu Thi Nguyet ◽  
Tuyen Phung Thi ◽  
Huong Lan Nguyen Thi ◽  
◽  
...  
Keyword(s):  
Curcuma Longa ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Antibacterial And Antifungal Activities ◽  
Agar Disc ◽  
Phytochemical Compounds ◽  
Ethanol Extracts ◽  
Antibacterial And Antifungal

The aim of this study was to identify and evaluate for antimicrobial activity and phytochemical properties of the extracts from some plants belonging to different species such as Piper betle L., Allium cepa L., Allium ascalonium L., Allium Sativum L., Curcuma longa L.; Paederia tomentosa L.; Wedelia chinensis M. and Chromolaena odorata L. These extracts have been prepared and the antimicrobial activities have been examined on some bacteria strains like Escherichia coli T1, Bacillus cereus M1; Salmonella sp. ST; Shigella sp. AT and one fungus strain Aspegillus flavus KN by agar disc diffusion method. Results showed that the extracts from total of 8 plant species have been inhibited the growth of all over the micro-organisms strains used in the test at a different ratio, but the ethanol extracts from Piper betle and Wedelia chinensis M. showed the highest effect. Also, the extracts then were tested for the presence of bioactive compounds. The results showed that there were tannins, flavones, carbohydrates, proteins, and oils in Wedelia chinensis M.extracts. In Piper betle extracts there were many phytochemical compounds including phenols, tannins, saponins, carbohydrates, proteins, resins, and oils.

scholarly journals A preliminary study on the antimicrobial activities and gas chromatography-mass spectrometry (GC-MS) analysis of the ethyl acetate extract of Dennettia tripetala G. Baker seeds

Bio-Research ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 1094-1102
Author(s):  
UF Babaiwa ◽  
SO Eraga ◽  
EO Ojugo ◽  
O Erharuyi ◽  
JO Akerele
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Traditional Use ◽  
Dennettia Tripetala

The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.  

scholarly journals Synthesis and Evaluation of Antimicrobial and Cytotoxic Activity of Oxathiine-Fused Quinone-Thioglucoside Conjugates of Substituted 1,4-Naphthoquinones

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3577
Author(s):  
Yuri E. Sabutski ◽  
Ekaterina S. Menchinskaya ◽  
Ludmila S. Shevchenko ◽  
Ekaterina A. Chingizova ◽  
Artur R. Chingizov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Hydroxyl Group ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
High Cytotoxic Activity ◽  
Derivatives Of

A series of new tetracyclic oxathiine-fused quinone-thioglycoside conjugates based on biologically active 1,4-naphthoquinones and 1-mercapto derivatives of per-O-acetyl d-glucose, d-galactose, d-xylose, and l-arabinose have been synthesized, characterized, and evaluated for their cytotoxic and antimicrobial activities. Six tetracyclic conjugates bearing a hydroxyl group in naphthoquinone core showed high cytotoxic activity with EC50 values in the range of 0.3 to 0.9 μM for various types of cancer and normal cells and no hemolytic activity up to 25 μM. The antimicrobial activity of conjugates was screened against Gram-positive bacteria (Staphylococcus aureus, Bacillus cereus), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), and fungus Candida albicans by the agar diffusion method. The most effective juglone conjugates with d-xylose or l-arabinose moiety and hydroxyl group at C-7 position of naphthoquinone core at concentration 10 µg/well showed antimicrobial activity comparable with antibiotics vancomicin and gentamicin against Gram-positive bacteria strains. In liquid media, juglone-arabinosidic tetracycles showed highest activity with MIC 6.25 µM. Thus, a positive effect of heterocyclization with mercaptosugars on cytotoxic and antimicrobial activity for group of 1,4-naphthoquinones was shown.

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

Trigona laeviceps Propolis from Thailand: Antimicrobial, Antiproliferative and Cytotoxic Activities

2009 ◽  
Vol 37 (05) ◽  
pp. 855-865 ◽  
Author(s):  
Supawadee Umthong ◽  
Songchan Puthong ◽  
Chanpen Chanchao
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
E Coli ◽  
Anticancer Properties ◽  
Crude Extracts ◽  
Sw620 Cells

Propolis is one of the natural bee products which has long been used as a crude preventative and prophylactic medicine, and has been reported to possess antibacterial, antiviral, anti-inflammatory, antioxidative and anticancer properties. Propolis of the stingless bee, Trigona laeviceps, was extracted by water or methanol at 35% (w/v) yielding a crude water or a methanolic extract at 60 and 80 mg/ml, respectively, which is 17.1 and 22.9% (w/w) of the total propolis, respectively. The antimicrobial activity of both crude extracts was assayed on four selected pathogenic microbes by using the agar well diffusion method. The results suggested that both water and methanolic crude extracts have some antimicrobial activities, water extract has greater antimicrobial activity than methanolic extract. The relative order of sensitivity of the four microbes were, however, the same between the two extracts from the most to least sensitive, S. aureus > E. coli ≫ C. albicans ⋙ A. niger, with indeed no observed growth inhibition of A. niger at all. Antiproliferative and cytotoxic affects were tested on the colon carcinoma cell line, SW620, using the three parameters: (1) MTT assay; (2) cell morphology; and (3) the fragmentation of genomic DNA. The water extract of propolis showed a higher antiproliferative activity than that of methanolic extract to SW620 cells, additionally both appeared to cause cell death by necrosis.

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