scholarly journals A Comparison of Tissue Engineering Scaffolds Incorporated with Manuka Honey of Varying UMF

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Katherine R. Hixon ◽  
Tracy Lu ◽  
Sarah H. McBride-Gagyi ◽  
Blythe E. Janowiak ◽  
Scott A. Sell
Keyword(s):  
Hydrogen Peroxide ◽  
Tissue Engineering ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Cell Number ◽  
Bacterial Clearance ◽  
Manuka Honey ◽  
Tissue Engineering Scaffolds ◽  
E Coli ◽  
Electrospun Scaffolds

Purpose. Manuka honey (MH) is an antibacterial agent specific to the islands of New Zealand containing both hydrogen peroxide and a Unique Manuka Factor (UMF). Although the antibacterial properties of MH have been studied, the effect of varying UMF of MH incorporated into tissue engineered scaffolds have not. Therefore, this study was designed to compare silk fibroin cryogels and electrospun scaffolds incorporated with a 5% MH concentration of various UMF.Methods. Characteristics such as porosity, bacterial clearance and adhesion, and cytotoxicity were compared.Results. Pore diameters for all cryogels were between 51 and 60 µm, while electrospun scaffolds were 10 µm. Cryogels of varying UMF displayed clearance of approximately 0.16 cm forE. coliandS. aureus. In comparison, the electrospun scaffolds clearance ranged between 0.5 and 1 cm. A glucose release of 0.5 mg/mL was observed for the first 24 hours by all scaffolds, regardless of UMF. With respect to cytotoxicity, neither scaffold caused the cell number to drop below 20,000.Conclusions. Overall, when comparing the effects of the various UMF within the two scaffolds, no significant differences were observed. This suggests that the fabricated scaffolds in this study displayed similar bacterial effects regardless of the UMF value.

scholarly journals Investigating Manuka Honey Antibacterial Properties When Incorporated into Cryogel, Hydrogel, and Electrospun Tissue Engineering Scaffolds

Gels ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 21 ◽  
Author(s):  
Hixon ◽  
Bogner ◽  
Ronning-Arnesen ◽  
Janowiak ◽  
Sell
Keyword(s):  
Bacterial Adhesion ◽  
Three Dimensional ◽  
Antibacterial Properties ◽  
Extended Period ◽  
Treatment Method ◽  
Cellular Adhesion ◽  
Bacterial Clearance ◽  
Manuka Honey ◽  
Tissue Engineering Scaffolds ◽  
Electrospun Scaffolds

Honey is well-known for its wound healing capability and Manuka honey (MH) contains a unique Manuka factor, providing an additional antibacterial agent. Previously, there has not been a practical way to apply MH to a wound site, which renders treatment for an extended period extremely difficult. Tissue-engineered scaffolds offer an alternative treatment method to standard dressings by providing varying geometries to best treat the specific tissue. MH was incorporated into cryogels, hydrogels, and electrospun scaffolds to assess the effect of scaffold geometry on bacterial clearance and adhesion, as well as cellular adhesion. Electrospun scaffolds exhibited a faster release due to the nanoporous fibrous geometry which led to a larger partial bacterial clearance as compared to the more three-dimensional cryogels (CG) and hydrogels (HG). Similarly, the fast release of MH from the electrospun scaffolds resulted in reduced bacterial adhesion. Overall, the fast MH release of the electrospun scaffolds versus the extended release of the HG and CG scaffolds provides differences in cellular/bacterial adhesion and advantages for both short and long-term applications, respectively. This manuscript provides a comparison of the scaffold pore structures as well as bacterial and cellular properties, providing information regarding the relationship between varying scaffold geometry and MH efficacy.

Durable Antibacterial Finish on Cotton Fabrics with PAMAM/Ag+

2011 ◽  
Vol 332-334 ◽  
pp. 77-80 ◽  
Author(s):  
Chuan Jie Zhang ◽  
Hong Yang ◽  
Yun Liu ◽  
Ping Zhu
Keyword(s):  
Cotton Fabric ◽  
Antibacterial Agent ◽  
Antibacterial Property ◽  
Breaking Strength ◽  
Antibacterial Properties ◽  
Pamam Dendrimers ◽  
Cotton Fabrics ◽  
E Coli ◽  
Strength Retention ◽  
Better Than

Cotton fabric with excellent antibacterial properties was obtained by treated with polyamide-amine (PAMAM) dendrimers as a carrier and silver nitrate as an antibacterial agent. The antibacterial cotton fabrics were prepared by the methods of one-bath process and two-bath process. Antibacterial activity of cotton fabrics treated by two different methods was good, but the antibacterial durability of cotton fabric treated with two-bath process was better than that treated with one-bath process. After 50 washing cycles, cotton fabric treated with two-bath process still had good antibacterial property and its inhibitory rate to Gram-positive S. aureus and Gram-negative E. coli was over 99 %. It was found that the breaking strength retention of finished cotton fabrics was 85.83 % and the decrease of cotton fabrics’ whiteness index was about 15 %.

Novel Benzotriazole acetamide derivatives as Benzo-Fused Five-Membered Nitrogen-Containing Heterocycle- Insilico Screening, Molecular Docking, and Synthesis

2021 ◽  
Vol 18 ◽  
Author(s):  
Patil Tejaswini D. ◽  
Amrutkar Sunil V.
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Docking ◽  
Active Site ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Dna Gyrase ◽  
Antibacterial Drug ◽  
E Coli ◽  
Fast Development ◽  
Subunit B

Background: DNA gyrase subunit B (1KZN) is an attractive target for antibacterial drug development because of its role in DNA replication. The fast development of antimicrobial medication resistance necessitates the quick discovery of new antimicrobial medicines. Objective: The goal of this research is to design, synthesize, and discover benzo-fused five-membered nitrogen-containing heterocycles that bind to DNA gyrase subunit B via molecular docking (1KZN). Methods: Based on literature research, 2-(1H-1,2,3-Benzotriazol-1-yl)-N-substituted acetamide was synthesized using an efficient method. All synthesized compounds were evaluated for antibacterial activity against three distinct organisms: E. coli, Pseudomonas aeruginosa, Staphylococcus aureus. In a docking investigation, the chemical interacts with the active site of DNA gyrase subunit B (1KZN), indicating that it might have antibacterial action. Conclusion: According to the findings of this research, the compounds 3d and 3f show antibacterial properties. For Staphylococcus aureus, 3c has the potential to be an antibacterial agent.

scholarly journals Synthesis, Characterization and Antibacterial Activity of Carbamate Derivatives of Isatin

2018 ◽  
Vol 34 (4) ◽  
pp. 2026-2030 ◽  
Author(s):  
Sarrah Sattar Jabbar
Keyword(s):  
Amino Acids ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
E Coli ◽  
Good Antibacterial Activity ◽  
Diffusion Technique ◽  
Derivatives Of ◽  
Isatin Derivatives

In search of novel antibacterial agent, a series of new isatin derivatives (3a-d) have been synthesized by condensation isatin (2,3-indolinendione) with piperidine (hexahydropyridine), hydrazine hydrate and Boc-amino acids respectively. Compounds synthesized have been characterized by IR spectroscopy and elemental analysis. In addition, the in vitro antibacterial properties have been tested against E. coli, P. aeruginosa, and Bacillus cereus, S. aureus by employing the well diffusion technique. A majority of the synthesized compounds were showing good antibacterial activity and from comparisons of the compounds, compound 3d has been determined to be the most active compound.

scholarly journals Antimicrobial Effect and Cytotoxic Evaluation of Mg-Doped Hydroxyapatite Functionalized with Au-Nano Rods

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1099
Author(s):  
Domenico Franco ◽  
Giovanna Calabrese ◽  
Salvatore Petralia ◽  
Giulia Neri ◽  
Carmelo Corsaro ◽  
...  
Keyword(s):  
Bone Matrix ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Cell Number ◽  
Bacterial Reduction ◽  
E Coli ◽  
Good Biocompatibility ◽  
Inorganic Mineral

Hydroxyapatite (HA) is the main inorganic mineral that constitutes bone matrix and represents the most used biomaterial for bone regeneration. Over the years, it has been demonstrated that HA exhibits good biocompatibility, osteoconductivity, and osteoinductivity both in vitro and in vivo, and can be prepared by synthetic and natural sources via easy fabrication strategies. However, its low antibacterial property and its fragile nature restricts its usage for bone graft applications. In this study we functionalized a MgHA scaffold with gold nanorods (AuNRs) and evaluated its antibacterial effect against S. aureus and E. coli in both suspension and adhesion and its cytotoxicity over time (1 to 24 days). Results show that the AuNRs nano-functionalization improves the antibacterial activity with 100% bacterial reduction after 24 h. The toxicity study, however, indicates a 4.38-fold cell number decrease at 24 days. Although further optimization on nano-functionalization process are needed for cytotoxicity, these data indicated that Au-NRs nano-functionalization is a very promising method for improving the antibacterial properties of HA.

scholarly journals Fabrication and Degradation of Electrospun Scaffolds from L-Tyrosine-Based Polyurethane Blends for Tissue Engineering Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Michael Spagnuolo ◽  
Lingyun Liu
Keyword(s):  
Tissue Engineering ◽  
Hydrolytic Degradation ◽  
Practical Method ◽  
Electrospinning Process ◽  
Diameter Distribution ◽  
Chemical Compositions ◽  
Tissue Engineering Scaffolds ◽  
Electrospun Scaffolds ◽  
Degradation Rates ◽  
Degradation Pattern

It is important to control the degradation rate of a tissue-engineered scaffold so that the scaffold will degrade in an appropriate matching rate as the tissue cells grow in. A set of potential tissue engineering scaffolds with controllable rates of degradation were fabricated from blends of two biocompatible, biodegradable L-tyrosine-based polyurethanes (PEG1000-HDI-DTH and PCL1250-HDI-DTH) using the electrospinning process. The scaffolds were characterized by mat morphology, fiber diameter, diameter distribution, pore size, and hydrolytic degradation behavior. The majority of the scaffolds, despite having radically different chemical compositions, possessed no statistical difference with pore sizes and fiber diameters. The degradation pattern observed indicated that scaffolds consisting of a greater mass percentage of PEG1000-HDI-DTH decayed to a greater extent than those containing higher concentrations of PCL1250-HDI-DTH. The degradation rates of the electrospun scaffolds were much higher than those of the thin cast films with same compositions. These patterns were consistent through all blends. The work demonstrates one practical method of controlling the degradation of biopolymer scaffolds without significantly affecting an intended morphology.

scholarly journals Enhanced Antibacterial Activity of Lactoperoxidase–Thiocyanate–Hydrogen Peroxide System in Reduced-Lactose Milk Whey

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Ahmad Ni’matullah Al-Baarri ◽  
Novia Tri Damayanti ◽  
Anang Mohamad Legowo ◽  
İsmail Hakkı Tekiner ◽  
Shigeru Hayakawa
Keyword(s):  
Hydrogen Peroxide ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Enzymatic Reaction ◽  
Bovine Milk ◽  
Milk Whey ◽  
E Coli ◽  
Lactoperoxidase System ◽  
Skimmed Milk ◽  
Derivative Products

The product of the lactoperoxidase system (LPOS) has been developed as a preservative agent to inhibit foodborne bacteria, but its action was, heretofore, limited to several original compounds in milk. This research was conducted to analyze the application of the lactoperoxidase system against Escherichia coli in fresh bovine milk and its derivative products to determine the strength of antibacterial activity. Lactoperoxidase was purified from bovine whey using the SP Sepharose Big Beads Column. The enzymatic reaction involving lactoperoxidase, thiocyanate, and hydrogen peroxide was used to generate the antibacterial agent from LPOS. This solution was then added to milk, skimmed milk, untreated whey, reduced-LPO whey, reduced-lactose whey, and high-lactose solution containing E. coli at an initial count of 6.0 log CFU/mL. LPOS showed the greatest reduction of bacteria (1.68 ± 0.1 log CFU/mL) in the reduced-lactose whey among the products tested. This result may lead to a method for enhancement of the antimicrobial activity of LPOS in milk and derived products.

scholarly journals Enhanced Antibacterial Activity of MGOTM Manuka Honey complexed with a- cyclodextrin (Manuka Honey with CycloPowerTM)

2014 ◽  
Vol 4 (5) ◽  
pp. 172 ◽  
Author(s):  
Simon Swift ◽  
Lynne M. Chepulis ◽  
Benedict Uy ◽  
Fiona J Radcliff
Keyword(s):  
Antibacterial Agent ◽  
Time Course ◽  
Antibacterial Properties ◽  
Sublethal Concentration ◽  
Bioactive Components ◽  
Manuka Honey ◽  
Naturally Occurring ◽  
Health Promoting ◽  
H Pylori ◽  
Improve Stability

Background: Manuka honey is recognized for it’s health-promoting properties and it’s use in medicine is well documented. However, the actions of Manuka honey are limited by rapid digestion and the inactivation of bioactive components such as methylglyoxal. Cyclodextrins are naturally occurring glucose rings that improve stability and bioactivity of products. This study investigates the tolerability and bioactivity of a-cyclodextrin-complexed Manuka honey called Manuka Honey with CycloPower TM.Methods: The antibacterial properties of Manuka honey complexed with a-cyclodextrin (Manuka honey with CyclopowerTM) were compared to uncomplexed Manuka honey against a range of common organisms using standard measurements of minimum inhibitory (MIC) and bactericidal (MBC) concentrations. Time course growth measurements were determined using a sublethal concentration of 2% w (honey solids)/v and measuring the area under the growth curve.Results: In tube MIC assays, Manuka honey completely inhibited Staphylococcus aureus (MSSA and MRSA), Streptococcus pyogenes, Helicobacter pylori and Moraxella catarrhalis at concentrations of 10% w/v or less, with MIC values decreasing as the methylglyoxal content of the honeys increased from 100 to 550 mg/kg. MIC values at a given methlyglyoxal level were also decreased for S. pyogenes, M. catarrhalis and H. pylori by complexing the Manuka honey with a-cyclodextrin. Pseudomonas aeruginosa was not inhibited by any of the Manuka honey or Cyclopower treatments at the concentrations tested (2-10% w/v). Manuka honey with CyclopowerTM had an increased bacteriostatic action against S. aureus, MRSA and P. aeruginosa compared with Manuka honey.Conclusions: This study concludes that Manuka honey is an effective antibacterial agent that can be enhanced by complexing with a-cyclodextrin.Keywords: Manuka honey, methylglyoxal, a-cyclodextrin, antimicrobial

scholarly journals Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 448 ◽  
Author(s):  
Azadeh Saberi ◽  
Farzaneh Jabbari ◽  
Payam Zarrintaj ◽  
Mohammad Reza Saeb ◽  
Masoud Mozafari
Keyword(s):  
Tissue Engineering ◽  
Intracellular Signaling ◽  
Cellular Response ◽  
Antibacterial Properties ◽  
Research Progress ◽  
Cellular Activity ◽  
Tissue Engineering Scaffolds ◽  
Electrically Conductive ◽  
Conductive Materials ◽  
Selection Of

Tissue engineering endeavors to regenerate tissues and organs through appropriate cellular and molecular interactions at biological interfaces. To this aim, bio-mimicking scaffolds have been designed and practiced to regenerate and repair dysfunctional tissues by modifying cellular activity. Cellular activity and intracellular signaling are performances given to a tissue as a result of the function of elaborated electrically conductive materials. In some cases, conductive materials have exhibited antibacterial properties; moreover, such materials can be utilized for on-demand drug release. Various types of materials ranging from polymers to ceramics and metals have been utilized as parts of conductive tissue engineering scaffolds, having conductivity assortments from a range of semi-conductive to conductive. The cellular and molecular activity can also be affected by the microstructure; therefore, the fabrication methods should be evaluated along with an appropriate selection of conductive materials. This review aims to address the research progress toward the use of electrically conductive materials for the modulation of cellular response at the material-tissue interface for tissue engineering applications.

scholarly journals Preliminary Investigation and Characterization of Electrospun Polycaprolactone and Manuka Honey Scaffolds for Dermal Repair

2015 ◽  
Vol 10 (4) ◽  
pp. 155892501501000 ◽  
Author(s):  
Benjamin A Minden-Birkenmaier ◽  
Rachel M Neuhalfen ◽  
Blythe E Janowiak ◽  
Scott A. Sell
Keyword(s):  
Wound Healing ◽  
Transmission Rate ◽  
Preliminary Investigation ◽  
Manuka Honey ◽  
Gram Negative ◽  
E Coli ◽  
Electrospun Scaffolds ◽  
Group B ◽  
Phosphate Buffered Saline

This study focused on the characterization of Manuka honey-containing poly(ε-caprolactone) (PCL) nanofiber scaffolds with regards to wound healing. Scaffolds were electrospun from 1, 5, 10, and 20% v/v Manuka honey solutions. Scaffolds were subjected to ethanol disinfection and soaked in phosphate-buffered saline (PBS) for various timepoints, and scaffold morphology and honey release was quantified. Scaffolds showed increased water vapor transmission rate (WVTR) with scaffold soak time, indicating an increase in evaporation due to enhanced osmotic potential of the scaffolds. Mechanical testing indicated lower elasticity and strength with honey incorporation, but showed no significant change in material degradation rate with the presence of honey over a 28 day PBS soak. Fibroblast studies showed honey incorporation increased cell infiltration into the scaffold, but scaffold conditioned media did not induce significant chemotaxis towards the scaffold. Honey incorporation also demonstrated an increase in fibroblast proliferation when in direct contact with the scaffolds. Bacterial clearance from pure honey was observed in both Gram positive Streptococcus agalactiae (Group B Streptocococcus) and Gram negative Escherichia coli ( E. coli), but honey scaffolds demonstrated significant clearance in only the Gram negative E. coli. While further investigation is needed, this preliminary study demonstrates the wound-healing potential of Manuka honey-loaded electrospun scaffolds.

Sign in / Sign up

Export Citation Format

Share Document