scholarly journals Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 448 ◽  
Author(s):  
Azadeh Saberi ◽  
Farzaneh Jabbari ◽  
Payam Zarrintaj ◽  
Mohammad Reza Saeb ◽  
Masoud Mozafari
Keyword(s):  
Tissue Engineering ◽  
Intracellular Signaling ◽  
Cellular Response ◽  
Antibacterial Properties ◽  
Research Progress ◽  
Cellular Activity ◽  
Tissue Engineering Scaffolds ◽  
Electrically Conductive ◽  
Conductive Materials ◽  
Selection Of

Tissue engineering endeavors to regenerate tissues and organs through appropriate cellular and molecular interactions at biological interfaces. To this aim, bio-mimicking scaffolds have been designed and practiced to regenerate and repair dysfunctional tissues by modifying cellular activity. Cellular activity and intracellular signaling are performances given to a tissue as a result of the function of elaborated electrically conductive materials. In some cases, conductive materials have exhibited antibacterial properties; moreover, such materials can be utilized for on-demand drug release. Various types of materials ranging from polymers to ceramics and metals have been utilized as parts of conductive tissue engineering scaffolds, having conductivity assortments from a range of semi-conductive to conductive. The cellular and molecular activity can also be affected by the microstructure; therefore, the fabrication methods should be evaluated along with an appropriate selection of conductive materials. This review aims to address the research progress toward the use of electrically conductive materials for the modulation of cellular response at the material-tissue interface for tissue engineering applications.

Research progress and prospects of tissue engineering scaffolds for spinal cord injury repair and protection

2019 ◽  
Vol 14 (9) ◽  
pp. 887-898
Author(s):  
Zhanjun Ma ◽  
Yubao Lu ◽  
Yang Yang ◽  
Jing Wang ◽  
Xuewen Kang
Keyword(s):  
Spinal Cord ◽  
Tissue Engineering ◽  
Spinal Cord Injury ◽  
Research Progress ◽  
Comprehensive Treatment ◽  
Biological Factors ◽  
Tissue Engineering Scaffolds ◽  
Biological Scaffolds ◽  
Injury Repair ◽  
Cord Injury

Spinal cord injury (SCI) is one of the leading causes of global disability. However, there are currently no effective clinical treatments for SCI. Repair of SCI is essential but poses great challenges. As a comprehensive treatment program combining biological scaffolds, seed cells and drugs or biological factors, tissue engineering has gradually replaced the single transplantation approach to become a focus of research that brings new opportunities for the clinical treatment of SCI.

scholarly journals A Comparison of Tissue Engineering Scaffolds Incorporated with Manuka Honey of Varying UMF

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Katherine R. Hixon ◽  
Tracy Lu ◽  
Sarah H. McBride-Gagyi ◽  
Blythe E. Janowiak ◽  
Scott A. Sell
Keyword(s):  
Hydrogen Peroxide ◽  
Tissue Engineering ◽  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Cell Number ◽  
Bacterial Clearance ◽  
Manuka Honey ◽  
Tissue Engineering Scaffolds ◽  
E Coli ◽  
Electrospun Scaffolds

Purpose. Manuka honey (MH) is an antibacterial agent specific to the islands of New Zealand containing both hydrogen peroxide and a Unique Manuka Factor (UMF). Although the antibacterial properties of MH have been studied, the effect of varying UMF of MH incorporated into tissue engineered scaffolds have not. Therefore, this study was designed to compare silk fibroin cryogels and electrospun scaffolds incorporated with a 5% MH concentration of various UMF.Methods. Characteristics such as porosity, bacterial clearance and adhesion, and cytotoxicity were compared.Results. Pore diameters for all cryogels were between 51 and 60 µm, while electrospun scaffolds were 10 µm. Cryogels of varying UMF displayed clearance of approximately 0.16 cm forE. coliandS. aureus. In comparison, the electrospun scaffolds clearance ranged between 0.5 and 1 cm. A glucose release of 0.5 mg/mL was observed for the first 24 hours by all scaffolds, regardless of UMF. With respect to cytotoxicity, neither scaffold caused the cell number to drop below 20,000.Conclusions. Overall, when comparing the effects of the various UMF within the two scaffolds, no significant differences were observed. This suggests that the fabricated scaffolds in this study displayed similar bacterial effects regardless of the UMF value.

Osteoblast Adhesion on Tissue Engineering Scaffolds Made by Bio-Manufacturing Techniques

2005 ◽  
Author(s):  
T. Dutta Roy ◽  
J. J. Stone ◽  
W. Sun ◽  
E. H. Cho ◽  
S. J. Lockett ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Focal Adhesion ◽  
Cellular Response ◽  
Focal Adhesions ◽  
Cellular Adhesion ◽  
Tissue Engineering Scaffolds ◽  
3D Scaffolds ◽  
Imaging Results ◽  
Proliferation And Differentiation ◽  
Manufacturing Techniques

Scientific exploration into understanding and developing relationships between three-dimensional (3D) scaffolds prepared by rapid prototyping (RP) and cellular response has focused primarily on end results targeting osteoblast proliferation and differentiation. Here at the National Institute of Standards and Technology (NIST), we take a systems approach to developing relationships between material properties and quantitative biological responses. This study in particular focuses on the screening of parameters controlled by RP techniques and their ability to trigger signalling events leading to cell adhesion. This pioneering research in our group also characterizes the in vitro cell-material interactions of 2D films and 3D scaffolds. From there, one can postulate on contributory factors leading to cell migration, proliferation, and differentiation. In summary, we believe that the quantitative information from this fundamental investigation will enhance our knowledge of the interactions between cells and 3D material interfaces with respect to formation of focal adhesions. This work consists of two sections — the application of imaging techniques for 3D characterization of properties and culturing of osteoblasts for size and shape determination. This includes quantifying the number of focal adhesion sites. We are using 3D RP polycaprolactone (PCL) scaffolds as this surrogate model in which to compare 2D to 3D material performance and cell interactions. Using RP bio-manufacturing techniques to fabricate tissue engineering scaffolds allows for control of pore size, strut size, and layer thickness, therefore providing adjustable parameters to study which can potentially influence, or even dynamically modulate, cellular adhesion. Imaging results after culturing for 24 h showed differences in cell morphology and spreading relative to the different structures. The focal adhesion response also varied, indicating an apparent loss of organization in 3D scaffolds compared to 2D surfaces. See Results and Discussion for details.

scholarly journals 3D Printing of Conductive Tissue Engineering Scaffolds Containing Polypyrrole Nanoparticles with Different Morphologies and Concentrations

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2491 ◽  
Author(s):  
Chunyang Ma ◽  
Le Jiang ◽  
Yingjin Wang ◽  
Fangli Gang ◽  
Nan Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Three Dimensional ◽  
Solvent Casting ◽  
Tissue Engineering Scaffolds ◽  
New Era ◽  
Conductive Materials ◽  
Conductive Film ◽  
Traditional Preparation ◽  
Natural Tissue

Inspired by electrically active tissues, conductive materials have been extensively developed for electrically active tissue engineering scaffolds. In addition to excellent conductivity, nanocomposite conductive materials can also provide nanoscale structure similar to the natural extracellular microenvironment. Recently, the combination of three-dimensional (3D) printing and nanotechnology has opened up a new era of conductive tissue engineering scaffolds exhibiting optimized properties and multifunctionality. Furthermore, in the case of two-dimensional (2D) conductive film scaffolds such as periosteum, nerve membrane, skin repair, etc., the traditional preparation process, such as solvent casting, produces 2D films with defects of unequal bubbles and thickness frequently. In this study, poly-l-lactide (PLLA) conductive scaffolds incorporated with polypyrrole (PPy) nanoparticles, which have multiscale structure similar to natural tissue, were prepared by combining extrusion-based low-temperature deposition 3D printing with freeze-drying. Furthermore, we creatively integrated the advantages of 3D printing and solvent casting and successfully developed a 2D conductive film scaffold with no bubbles, uniform thickness, and good structural stability. Subsequently, the effects of concentration and morphology of PPy nanoparticles on electrical properties and mechanical properties of 3D conductive scaffolds and 2D conductive films scaffolds have been studied, which provided a new idea for the design of both 2D and 3D electroactive tissue engineering scaffolds.

Selective HIFU Foaming to Fabricate Porous Polymer for Tissue Engineering Scaffolds

2006 ◽  
Author(s):  
Hai Wang ◽  
Wei Li
Keyword(s):  
Tissue Engineering ◽  
Cellular Response ◽  
Focused Ultrasound ◽  
Low Cost ◽  
Hierarchical Structures ◽  
Polymeric Materials ◽  
Scanning Speed ◽  
High Intensity Focused Ultrasound ◽  
Porous Polymer ◽  
Tissue Engineering Scaffolds

A novel technique is presented in this paper for the fabrication of tissue engineering scaffolds using the High Intensity Focused Ultrasound (HIFU). This acoustic method is a solvent-free, highly efficient and low cost process that has the potential in scaffold-based tissue engineering. HIFU fabrication technique is capable of creating hierarchically-structured porous polymeric materials, which have various topographical features at different length scales. This will in turn affect the cellular response and behavior of certain type of cells, such as the integration and growth of smooth muscle cells (SMCs). In this study, the effect of HIFU porous polymer fabrication was investigated. Scanning-mode HIFU insonation was performed in the HIFU polymer foaming experiments. The acoustic power and the scanning speed were chosen as the parameters and varied in different groups of experiments. The created microstructures were characterized using the scanning electron microscopy (SEM). The fabricated samples were used for cell culture studies with human aortic SMCs (Passage 4). It was found that the selective HIFU foaming process could be used to create hierarchical structures by choosing appropriate ultrasound parameters. The SMCs were viable on the HIFU-created porous PMMA specimens, and the topographical nature of a HIFU-created porous structure affected the cellular response of SMCs.

scholarly journals Investigations of Graphene and Nitrogen-Doped Graphene Enhanced Polycaprolactone 3D Scaffolds for Bone Tissue Engineering

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 929
Author(s):  
Weiguang Wang ◽  
Jun-Xiang Chen ◽  
Yanhao Hou ◽  
Paulo Bartolo ◽  
Wei-Hung Chiang
Keyword(s):  
Electrical Conductivity ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Pristine Graphene ◽  
Tissue Engineering Scaffolds ◽  
Electrically Conductive ◽  
Nitrogen Doped ◽  
Nitrogen Doped Graphene ◽  
Doped Graphene

Scaffolds play a key role in tissue engineering applications. In the case of bone tissue engineering, scaffolds are expected to provide both sufficient mechanical properties to withstand the physiological loads, and appropriate bioactivity to stimulate cell growth. In order to further enhance cell–cell signaling and cell–material interaction, electro-active scaffolds have been developed based on the use of electrically conductive biomaterials or blending electrically conductive fillers to non-conductive biomaterials. Graphene has been widely used as functioning filler for the fabrication of electro-active bone tissue engineering scaffolds, due to its high electrical conductivity and potential to enhance both mechanical and biological properties. Nitrogen-doped graphene, a unique form of graphene-derived nanomaterials, presents significantly higher electrical conductivity than pristine graphene, and better surface hydrophilicity while maintaining a similar mechanical property. This paper investigates the synthesis and use of high-performance nitrogen-doped graphene as a functional filler of poly(ɛ-caprolactone) (PCL) scaffolds enabling to develop the next generation of electro-active scaffolds. Compared to PCL scaffolds and PCL/graphene scaffolds, these novel scaffolds present improved in vitro biological performance.

Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

2019 ◽  
Author(s):  
AS Arampatzis ◽  
K Theodoridis ◽  
E Aggelidou ◽  
KN Kontogiannopoulos ◽  
I Tsivintzelis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Dressings ◽  
Active Ingredients ◽  
Tissue Engineering Scaffolds

Selection of tissue engineering matrix for cardiac repair medicine

2004 ◽  
Vol 52 (S 1) ◽  
Author(s):  
MN Giraud ◽  
H Tevaearai ◽  
C Receputo ◽  
U Nydegger ◽  
T Carrel
Keyword(s):  
Tissue Engineering ◽  
Cardiac Repair ◽  
Selection Of

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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