scholarly journals Mixed Infections ofHelicobacter pyloriIsolated from Patients with Gastrointestinal Diseases in Taiwan

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Chih-Ho Lai ◽  
Ju-Chun Huang ◽  
Chuan Chiang-Ni ◽  
Ju-Pi Li ◽  
Lii-Tzu Wu ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Gastrointestinal Diseases ◽  
Mixed Infections ◽  
Hostile Environment ◽  
High Genetic Diversity ◽  
Vacuolating Cytotoxin ◽  
Severity Of Disease ◽  
Cytotoxin Associated Gene A ◽  
H Pylori ◽  
Upper Gastrointestinal

Background. PersistentHelicobacter pyloriinfection may induce several upper gastrointestinal diseases. Two major virulence factors ofH. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution ofvacAandcag-pathogenicity island (cag-PAI) alleles varies inH. pyloriisolated from patients in different geographic regions.Aim. To assess the association between mixed infection ofH. pyloriclinical isolates from Taiwanese patients and the severity of gastrointestinal diseases.Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes.Results. The prevalence of mixed infections of allH. pylori-infected patients was 28.6% (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6%) was much higher than that with other gastrointestinal diseases (P<0.05).Conclusions.H. pylorimixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development.

scholarly journals Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Onelkis Feliciano ◽  
Oderay Gutierrez ◽  
Lidunka Valdés ◽  
Trini Fragoso ◽  
Ana Maria Calderin ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Allelic Variation ◽  
Gastrointestinal Diseases ◽  
Statistical Association ◽  
Similar Frequency ◽  
High Prevalence ◽  
H Pylori ◽  
Over 40 Years ◽  
Upper Gastrointestinal ◽  
Significant Statistical Association

Virulence factors ofHelicobacter pylorican predict the development of different gastroduodenal diseases. There are scarce reports in Cuba aboutH. pyloriisolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genesvacA,cagA, andiceAin sixty-eight patients diagnosed asH. pyloripositive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all theH. pyloristrains and evaluated by PCR-genotyping. ThevacAs1 allele,cagAgene, andiceA2allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multipleH. pylorigenotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed betweeniceA2allele and patients with non-peptic ulcer dyspepsia (NUD) (P=0.037) as well as virulence genotypes (s1,s1m2) and patients over 40 years old (P<0.05). In conclusion, the results demonstrated a high prevalence ofH. pylorivirulent genotypes in Cuban patients over 40 years old whileiceA2alleles demonstrated a good specificity in patients with NUD.

scholarly journals Campylobacter jejuni Cytolethal Distending Toxin C Exploits Lipid Rafts to Mitigate Helicobacter pylori-Induced Pathogenesis

2021 ◽  
Vol 8 ◽  
Author(s):  
Jia-Yin Yeh ◽  
Hwai-Jeng Lin ◽  
Chia-Jung Kuo ◽  
Chun-Lung Feng ◽  
Chia-Huei Chou ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Lipid Rafts ◽  
Campylobacter Jejuni ◽  
Nuclear Factor Kappa B ◽  
Gastrointestinal Diseases ◽  
Membrane Lipid ◽  
Cytolethal Distending Toxin ◽  
Vacuolating Cytotoxin ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Helicobacter pylori infection is associated with several gastrointestinal diseases, including gastritis, peptic ulcer, and gastrointestinal adenocarcinoma. Two major cytotoxins, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), interact closely with lipid rafts, contributing to H. pylori-associated disease progression. The Campylobacter jejuni cytolethal distending toxin consists of three subunits: CdtA, CdtB, and CdtC. Among them, CdtA and CdtC bind to membrane lipid rafts, which is crucial for CdtB entry into cells. In this study, we employed recombinant CdtC (rCdtC) to antagonize the functions of H. pylori cytotoxin in cells. Our results showed that rCdtC alleviates cell vacuolation induced by H. pylori VacA. Furthermore, rCdtC reduces H. pylori CagA translocation, which decreases nuclear factor kappa-B activation and interleukin-8 production, resulting in the mitigation of gastric epithelial cell inflammation. These results reveal that CdtC hijacks cholesterol to compete for H. pylori cytotoxin actions via lipid rafts, ameliorating H. pylori-induced pathogenesis.

Prevalence of Helicobacter pylori cagA and vacA Genes and Their Correlation with Gastrointestinal Diseases

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 151-156
Author(s):  
Amr M. El-Sabbagh ◽  
Ahmed H. Yassen ◽  
Maha M abdelsalam
Keyword(s):  
Helicobacter Pylori ◽  
Gastrointestinal Diseases ◽  
Vital Role ◽  
Gastroduodenal Disease ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
Gastrointestinal Illnesses ◽  
Clinical Illness ◽  
H Pylori ◽  
Variant Genes

Background: Helicobacter pylori (H. pylori) is a gram-negative bacterium, producing gastric ulcer, mild to severe gastritis, gastric carcinoma and lymphoma to the gastric mucosa-associated lymphoid tissue through many virulence influences. Among the virulence factors identified; vacuolating cytotoxin A (vacA) and cytotoxin-associated gene A (cagA) play an important role. Objective: In this study, we try to discover the relation between vacA variant and cagA genes with the clinical illness occurring in H. pylori patients Methodology: One hundred and forty patients were included in our study. Dual biopsy samples were taken from the stomach; one was examined by the urease test, and the other one was stored at −80°C for DNA extraction followed by PCR. The existence of H. pylori in the tissue was recognized by the existence of glmM gene and its detection by PCR. All the positive samples were additionally tested by PCR for the occurrence of cagA and vacA variant genes. Results: Our study demonstrated that cagA and vacA genes were found among 50% and 57% respectively of H. pylori patients complaining from gastrointestinal illnesses and that vacA s1/s2 was the main genotype found in H. pylori persons with gastroduodenal disease. Significant relation between vacA s1 gene and cagA gene was found. Conclusion: vacA s1 genotype has a vital role in upper gastrointestinal illnesses progress.

scholarly journals Colonization of Mexican Patients by MultipleHelicobacter pylori Strains with Different vacAand cagA Genotypes

1999 ◽  
Vol 37 (9) ◽  
pp. 3001-3004 ◽  
Author(s):  
Rosario Morales-Espinosa ◽  
Gonzalo Castillo-Rojas ◽  
Gerardo Gonzalez-Valencia ◽  
Sergio Ponce de León ◽  
Alejandro Cravioto ◽  
...  
Keyword(s):  
Signal Sequence ◽  
Virulence Determinants ◽  
Vacuolating Cytotoxin ◽  
Latin Americans ◽  
Coding Regions ◽  
Biopsy Specimens ◽  
Single Colony ◽  
Cytotoxin Associated Gene A ◽  
H Pylori ◽  
Cytotoxin Gene

Helicobacter pylori virulence determinants have not previously been studied in detail in Latin Americans with H. pylori infections. We characterized the vacA(vacuolating cytotoxin gene A) and cagA(cytotoxin-associated gene A) types of more than 400 single-colony isolates from 20 patients in Mexico City. For 17 patients H. pylori strains of two or more different vacAgenotypes were isolated from gastric biopsy specimens, indicating infection with two or more strains of H. pylori. The most frequent vacA genotype was s1b/m1. vacAdiversity was more marked than that described previously, in that isolates from seven patients had untypeable vacA midregions and isolates from nine patients had type s1 signal sequence coding regions which could not be further subtyped. Previously undescribedvacA type s2/m1 strains were found in five patients. All patients were infected with cagA-positive strains, but occasionally, these coexisted with small numbers ofcagA-negative strains. In conclusion, coinfection with multiple H. pylori strains is common in Mexico, andvacA in these strains is genetically more diverse than has been described in other populations.

scholarly journals Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ashwak M. F. Abu-Taleb ◽  
Randa S. Abdelattef ◽  
Amina A. Abdel-Hady ◽  
Farida H. Omran ◽  
Lobna A. El-korashi ◽  
...  
Keyword(s):  
Peptic Ulcer ◽  
Gastrointestinal Endoscopy ◽  
Gastrointestinal Diseases ◽  
Rapid Urease Test ◽  
University Hospitals ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
Gastric Biopsies ◽  
H Pylori ◽  
The Relationship

H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

scholarly journals Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Rania M. Kishk ◽  
Nashaat M. Soliman ◽  
Maha M. Anani ◽  
Nader Nemr ◽  
Ayman Salem ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Virulence Genes ◽  
Histopathological Examination ◽  
Virulence Gene ◽  
National Study ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

scholarly journals Prevalence of Tumor necrosis factor alpha inducing protein (tipα) gene of Helicobacter pylori and its association with Upper Gastrointestinal Diseases in India

2020 ◽  
Author(s):  
Shweta Mahant ◽  
Shubham Mehra ◽  
Ayushi Chhawchharia ◽  
Bipul Chandra Karmakar ◽  
Sangita Paul ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Indian Population ◽  
Gastrointestinal Diseases ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
H Pylori ◽  
Upper Gastrointestinal ◽  
Necrosis Factor

Abstract Background: Helicobacter pylori (H. pylori) is known to cause several gastro-duodenal diseases including chronic Gastritis, Peptic Ulcer disease and Gastric Cancer. Virulent genes of H. pylori like cagA, vacA are known to be responsible for the disease pathogenesis. But these virulence genes are not always found to be associated with disease outcome in all populations around the world. Relationship of H. pylori with Gastro Esophageal Reflux Diseases is controversial and uncertain. Tumor necrosis factor alpha inducing protein tipα is a new discovered virulent gene of H. pylori and is an inducer of certain cytokines and chemokines that are responsible for causing stomach cancer. Therefore, we conducted a study which aims to find the prevalence of tipα gene in the Indian population and its association with H. pylori related upper gastrointestinal diseases.Results: 267 clinical H. pylori isolates are included in our study for finding the prevalence of tipα gene and its association with cagA and vacA gene using PCR assay. The current study shows the prevalence rate of tipα gene in the Indian population to be 59.9%. Our study has found a significant association (p < 0.05) of tipα gene with Non Ulcer Dyspepsia (NUD) and also an association of cagA and vacA s1m1 with Gastritis and Duodenal Ulcer.Conclusion: Our study demonstrates for the first time the presence of tipα as virulent factor of H. pylori strain in Indian population isolated from patients suffering from upper gastrointestinal diseases. Further, tipα is significantly associated with NUD but not with other upper gastrointestinal diseases in India.

scholarly journals Prevalence of Helicobacter Pylori among Patients undergoing Gastrodudenoscopy in a Hospital in Western Nepal

2017 ◽  
Vol 5 (2) ◽  
pp. 69-73
Author(s):  
Raju Prasad Shakya ◽  
Sudeep Regmi ◽  
Suraj Adhikari
Keyword(s):  
Helicobacter Pylori ◽  
Gastrointestinal Symptoms ◽  
Gastrointestinal Diseases ◽  
Fisher Exact Test ◽  
Histopathological Diagnosis ◽  
Common Symptom ◽  
Major Health Problem ◽  
Gastrointestinal Bleed ◽  
H Pylori ◽  
Upper Gastrointestinal

Introduction: Helicobacter pylori (H. pylori) related chronic gastritis is a major health problem worldwide, specially in the developing countries. The prevalence of H. pylori infection has been reported to vary between and even within countries. There are limited data on this infection in Western Nepal. Our objective was to study the prevalence of H. pylori infection and its association with presenting complains and upper gastrointestinal diseases. Methods: Medical records of patients undergoing gastrodudenoscopy and biopsy for various upper gastrointestinal symptoms from 1st of January 2015 to 30th of June 2017 were reviewed for presence of H. pylori infection, demographics, indications for gastrodudenoscopy, and histopathology findings. T-test, Chi-square test, and Fisher exact test were applied. Results: Two hundred fifty six patients (135 male and 121 female) with a mean age of 47 (SD = 16.5) underwent gastroscopic biopsy and had an overall H. pylori prevalence of 24.6%. H. pylori infection was most commonly noted between 41 to 60 years of age. Gender did not seem to be significantly associated (p = 0.82) but gastrointestinal bleed was significantly associated with H. pylori infection (p = 0.006). The most common histopathological diagnosis was gastritis followed by gastrodudenitis; however, none of the diagnosis were found to be significantly associated with H. pylori infection. Conclusion: The overall prevalence of H. pylori infection was 24.6% and was most common between 40 to 60 years of age. Heart burn was the most common symptom and gastrointestinal bleed was the only significantly associated symptom with H. pylori infection.

Sign in / Sign up

Export Citation Format

Share Document