scholarly journals Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Onelkis Feliciano ◽  
Oderay Gutierrez ◽  
Lidunka Valdés ◽  
Trini Fragoso ◽  
Ana Maria Calderin ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Allelic Variation ◽  
Gastrointestinal Diseases ◽  
Statistical Association ◽  
Similar Frequency ◽  
High Prevalence ◽  
H Pylori ◽  
Over 40 Years ◽  
Upper Gastrointestinal ◽  
Significant Statistical Association

Virulence factors ofHelicobacter pylorican predict the development of different gastroduodenal diseases. There are scarce reports in Cuba aboutH. pyloriisolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genesvacA,cagA, andiceAin sixty-eight patients diagnosed asH. pyloripositive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all theH. pyloristrains and evaluated by PCR-genotyping. ThevacAs1 allele,cagAgene, andiceA2allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multipleH. pylorigenotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed betweeniceA2allele and patients with non-peptic ulcer dyspepsia (NUD) (P=0.037) as well as virulence genotypes (s1,s1m2) and patients over 40 years old (P<0.05). In conclusion, the results demonstrated a high prevalence ofH. pylorivirulent genotypes in Cuban patients over 40 years old whileiceA2alleles demonstrated a good specificity in patients with NUD.

scholarly journals Mixed Infections ofHelicobacter pyloriIsolated from Patients with Gastrointestinal Diseases in Taiwan

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Chih-Ho Lai ◽  
Ju-Chun Huang ◽  
Chuan Chiang-Ni ◽  
Ju-Pi Li ◽  
Lii-Tzu Wu ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Gastrointestinal Diseases ◽  
Mixed Infections ◽  
Hostile Environment ◽  
High Genetic Diversity ◽  
Vacuolating Cytotoxin ◽  
Severity Of Disease ◽  
Cytotoxin Associated Gene A ◽  
H Pylori ◽  
Upper Gastrointestinal

Background. PersistentHelicobacter pyloriinfection may induce several upper gastrointestinal diseases. Two major virulence factors ofH. pylori, vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA), are thought to be associated with the severity of disease progression. The distribution ofvacAandcag-pathogenicity island (cag-PAI) alleles varies inH. pyloriisolated from patients in different geographic regions.Aim. To assess the association between mixed infection ofH. pyloriclinical isolates from Taiwanese patients and the severity of gastrointestinal diseases.Methods. A total of 70 patients were enrolled in this study. Six distinct and well-separated colonies were isolated from each patient and 420 colonies were analyzed to determine the genotypes of virulence genes.Results. The prevalence of mixed infections of allH. pylori-infected patients was 28.6% (20/70). The rate of mixed infections in patients with duodenal ulcer (47.6%) was much higher than that with other gastrointestinal diseases (P<0.05).Conclusions.H. pylorimixed infections show high genetic diversity that may enhance bacterial adaptation to the hostile environment of the stomach and contribute to disease development.

scholarly journals Prevalence of Helicobacter pylori vacA, cagA, cagE1, cagE2, dupA and oipA Genotypes in Patients With Gastrointestinal Diseases

2020 ◽  
Author(s):  
Hossein Masoumi Asl ◽  
Ali Badamchi ◽  
Shima Javadinia ◽  
Siamak Khaleghi ◽  
Leila Tehraninia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Gastrointestinal Diseases ◽  
Cancer Group ◽  
Urease Test ◽  
High Prevalence ◽  
Vaca Gene ◽  
H Pylori ◽  
Positive Results ◽  
Gastroenterology Unit

Helicobacter pylori (H. pylori) is a bacterium that resides in the human stomach, which is associated with gastroduodenal diseases. We investigate the prevalence of cagA, vacA, oipA, cagE1, cagE2 and dupA genotypes in H. pylori isolated from patients with Gastric ulcer, duodenal ulcer, and Gastric Cancer. Collected 74 samples from the Gastroenterology Unit of the Rasool Akram Hospital were included in this study. Gastric disorders were identified by endoscopy .gastric cancer was further confirmed by histopathology. H. pylori were detected by the urease test. Subsequently, DNA was extracted from gastric tissue of the subjects with the CLO-test yielded positive results. In general, 74 patients with a mean age of 53.45 years (Range 22 to 86-year-old), including 45 men and 29 women, were studied. Among 74 H. pylori-positive patients, 70 (94.5%) patients were positive for the cagA gene. About 95.8% (23/24) of the patients with gastric carcinoma were dupA positive and VacA gene (91.8%). The oipA genotype was detected in 71 (96%) of H.pylori positive samples. This gene was more common in patients with gastritis rather than cancer group. Also, 97.2% of 74 H. pylori isolates were cagE2-positive. In 25 patients with PUD, the occurrence percent of cagA+/VacA+, cagA+/Vac- , cagA- /VacA+ and cagA- /VaxA- genotypes were found 80%, 12%, 4.2% and 4.2 respectively. The results of the present study suggest that a high prevalence of virulent factors could contribute to the risk of developing gastroduodenal diseases.

scholarly journals High prevalence of dna from non-H. pylori helicobacters in the gastric mucosa of venezuelan pet dogs and its histological alterations

2011 ◽  
Vol 53 (4) ◽  
pp. 207-212 ◽  
Author(s):  
Rito Polanco ◽  
Víctor Salazar ◽  
Nelson Reyes ◽  
María Alexandra García-Amado ◽  
Fabián Michelangeli
Keyword(s):  
Gastric Mucosa ◽  
16S Rrna ◽  
Gastrointestinal Disease ◽  
Rrna Gene ◽  
Fish Analysis ◽  
Normal Gastric Mucosa ◽  
16S Rrna Gene Sequences ◽  
High Prevalence ◽  
H Pylori ◽  
Upper Gastrointestinal

Non-H. pylori helicobacters (NHPH) have been demonstrated as gastric spiral-shaped bacteria in specimens obtained from dogs; however, their roles in the pathogenesis of upper gastrointestinal disease have not yet been clearly established. The purpose of this study was to evaluate the prevalence of NHPH DNA in the gastric mucosa of dogs and its association with histopathology. Helicobacter was detected through histopathological techniques, PCR, and FISH analysis from fundic biopsies of twenty dogs with or without signs of gastrointestinal disease. PCR and FISH were based on partial 16S rRNA gene sequences. Nineteen dogs showed mild to marked gastritis in the fundus, and only one dog had a healthy gastric mucosa. NHPH DNA was detected in 18 dogs with gastritis and one with normal gastric mucosa. However, there was no significant correlation between the presence of NHPH DNA and the degree of gastritis. These results show a high prevalence of NHPH DNA in the gastric mucosa of dogs from Venezuela. Further studies are necessary to determine a possible association between a specific NHPH species and the degree of gastritis.

scholarly journals Preoperative Endoscopy and Its Impact on Perioperative Management in Bariatric Surgery

2015 ◽  
Vol 32 (4) ◽  
pp. 238-242 ◽  
Author(s):  
Georg Wiltberger ◽  
Julian Nikolaus Bucher ◽  
Moritz Schmelzle ◽  
Albrecht Hoffmeister ◽  
Arne Dietrich
Keyword(s):  
Bariatric Surgery ◽  
Medical Treatment ◽  
Perioperative Management ◽  
Gastrointestinal Diseases ◽  
Upper Gastrointestinal Endoscopy ◽  
Upper Endoscopy ◽  
High Prevalence ◽  
Upper Gastrointestinal ◽  
Prospective Database

Background: The role of preoperative upper-gastrointestinal endoscopy for bariatric surgery is still understood only with controversy. The aim of this study was to evaluate the prevalence of endoscopic findings and its impact on perioperative management. Methods: Patients who underwent bariatric surgery at our center between 2010 and 2013 were systematically analyzed from a prospective database. Results: Two hundred and twelve patients with a median body mass index of 50 kg/m2 (range 29-87) underwent 216 bariatric procedures at our center between 2010 and 2013. All patients received preoperative upper endoscopy. In 159 cases (75%), the endoscopy was performed at our center. These cases were included in this study. In 37 cases (23%), no abnormal findings were detected. In 122 cases (76%), upper endoscopy revealed pathologies. No further treatment was necessary in 24 cases (15%). Medical treatment was changed in 81 cases (51%). The operation was delayed due to medical treatment and re-endoscopy in 13 cases (8%). The surgical approach was changed in 4 cases (3%). Conclusion: Routinely performed preoperative endoscopy before bariatric surgery revealed a high prevalence of gastrointestinal diseases with a significant impact on perioperative management in two thirds of the cases. Therefore, we recommend routine gastroscopy about 2-4 weeks prior to surgery.

scholarly journals Prevalence of Tumor necrosis factor alpha inducing protein (tipα) gene of Helicobacter pylori and its association with Upper Gastrointestinal Diseases in India

2020 ◽  
Author(s):  
Shweta Mahant ◽  
Shubham Mehra ◽  
Ayushi Chhawchharia ◽  
Bipul Chandra Karmakar ◽  
Sangita Paul ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Indian Population ◽  
Gastrointestinal Diseases ◽  
Necrosis Factor Alpha ◽  
Factor Alpha ◽  
H Pylori ◽  
Upper Gastrointestinal ◽  
Necrosis Factor

Abstract Background: Helicobacter pylori (H. pylori) is known to cause several gastro-duodenal diseases including chronic Gastritis, Peptic Ulcer disease and Gastric Cancer. Virulent genes of H. pylori like cagA, vacA are known to be responsible for the disease pathogenesis. But these virulence genes are not always found to be associated with disease outcome in all populations around the world. Relationship of H. pylori with Gastro Esophageal Reflux Diseases is controversial and uncertain. Tumor necrosis factor alpha inducing protein tipα is a new discovered virulent gene of H. pylori and is an inducer of certain cytokines and chemokines that are responsible for causing stomach cancer. Therefore, we conducted a study which aims to find the prevalence of tipα gene in the Indian population and its association with H. pylori related upper gastrointestinal diseases.Results: 267 clinical H. pylori isolates are included in our study for finding the prevalence of tipα gene and its association with cagA and vacA gene using PCR assay. The current study shows the prevalence rate of tipα gene in the Indian population to be 59.9%. Our study has found a significant association (p < 0.05) of tipα gene with Non Ulcer Dyspepsia (NUD) and also an association of cagA and vacA s1m1 with Gastritis and Duodenal Ulcer.Conclusion: Our study demonstrates for the first time the presence of tipα as virulent factor of H. pylori strain in Indian population isolated from patients suffering from upper gastrointestinal diseases. Further, tipα is significantly associated with NUD but not with other upper gastrointestinal diseases in India.

scholarly journals Prevalence of Helicobacter Pylori among Patients undergoing Gastrodudenoscopy in a Hospital in Western Nepal

2017 ◽  
Vol 5 (2) ◽  
pp. 69-73
Author(s):  
Raju Prasad Shakya ◽  
Sudeep Regmi ◽  
Suraj Adhikari
Keyword(s):  
Helicobacter Pylori ◽  
Gastrointestinal Symptoms ◽  
Gastrointestinal Diseases ◽  
Fisher Exact Test ◽  
Histopathological Diagnosis ◽  
Common Symptom ◽  
Major Health Problem ◽  
Gastrointestinal Bleed ◽  
H Pylori ◽  
Upper Gastrointestinal

Introduction: Helicobacter pylori (H. pylori) related chronic gastritis is a major health problem worldwide, specially in the developing countries. The prevalence of H. pylori infection has been reported to vary between and even within countries. There are limited data on this infection in Western Nepal. Our objective was to study the prevalence of H. pylori infection and its association with presenting complains and upper gastrointestinal diseases. Methods: Medical records of patients undergoing gastrodudenoscopy and biopsy for various upper gastrointestinal symptoms from 1st of January 2015 to 30th of June 2017 were reviewed for presence of H. pylori infection, demographics, indications for gastrodudenoscopy, and histopathology findings. T-test, Chi-square test, and Fisher exact test were applied. Results: Two hundred fifty six patients (135 male and 121 female) with a mean age of 47 (SD = 16.5) underwent gastroscopic biopsy and had an overall H. pylori prevalence of 24.6%. H. pylori infection was most commonly noted between 41 to 60 years of age. Gender did not seem to be significantly associated (p = 0.82) but gastrointestinal bleed was significantly associated with H. pylori infection (p = 0.006). The most common histopathological diagnosis was gastritis followed by gastrodudenitis; however, none of the diagnosis were found to be significantly associated with H. pylori infection. Conclusion: The overall prevalence of H. pylori infection was 24.6% and was most common between 40 to 60 years of age. Heart burn was the most common symptom and gastrointestinal bleed was the only significantly associated symptom with H. pylori infection.

scholarly journals Helicobacter pyloriin First Nations and Recent Immigrant Populations in Canada

2012 ◽  
Vol 26 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Nicola L Jones ◽  
Naoki Chiba ◽  
Carlo Fallone ◽  
Alan Thomson ◽  
Richard Hunt ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
First Nations ◽  
Cost Effective ◽  
Gastrointestinal Diseases ◽  
Population Based ◽  
Peptic Ulcers ◽  
Canadian Population ◽  
High Prevalence ◽  
H Pylori ◽  
Low Prevalence

The diminishing prevalence ofHelicobacter pyloriinfection among most segments of the Canadian population has led to changes in the etiologies and patterns of associated upper gastrointestinal diseases, including fewer peptic ulcers and their complications. Canadian Aboriginals and recent immigrants are among populations in which the prevalence ofH pyloriinfection remains high and, therefore, the health risks imposed byH pyloriremain a significant concern. Population-based strategies forH pylorieradication in groups with a low prevalence of infection are unlikely to be cost effective, but such measures are attractive in groups in which the prevalence rates of infection remain substantial. In addition to a lower prevalence of peptic ulcers and dyspepsia, the public health value of eradication may be particularly important if this leads to a reduction in the prevalence of gastric cancer in high prevalence groups. Therefore The Canadian Helicobacter Study Group held a conference that brought together experts in the field to address these issues, the results of which are reviewed in the present article. Canadians with the highest prevalence ofH pyloriinfection are an appropriate focus for considering the health advantages of eradicating persistent infection. In Canadian communities with a high prevalence of bothH pyloriand gastric cancer, there remains an opportunity to test the hypothesis thatH pyloriinfection is a treatable risk factor for malignancy.

scholarly journals Association betweenHelicobacter pyloriInfection and Risk of Osteoporosis in Elderly Taiwanese Women with Upper Gastrointestinal Diseases: A Retrospective Patient Record Review

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Shih-Chun Lin ◽  
Malcolm Koo ◽  
Kun-Wei Tsai
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Logistic Regression Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Gastrointestinal Diseases ◽  
Multivariate Logistic Regression ◽  
Female Patients ◽  
H Pylori ◽  
Record Review ◽  
Upper Gastrointestinal

Introduction. Helicobacter pylori (H. pylori)infection could lead to chronic local and systemic immune response. The resulting increase in proinflammatory cytokines could affect bone resorption and might increase the risk of osteoporosis. This study aimed to investigate the association betweenH. pyloriinfection and osteoporosis in elderly female patients with upper gastrointestinal diseases.Methods. A retrospective patient record review study was conducted in a regional teaching hospital in south Taiwan. Relevant information on female patients aged 65 and over who were diagnosed with diseases of esophagus, gastric ulcer, or duodenal ulcer during January 2008 to December 2010 were abstracted. Association betweenH. pyloriinfection and osteoporosis was evaluated using multivariate logistic regression analysis.Results. Of the 365 patients with a mean age of 77.3 years, 77 (21.1%) hadH. pyloriinfection and 101 (27.7%) had been diagnosed with osteoporosis. Multivariate logistic regression analysis revealed that osteoporosis was significantly associated withH. pyloriinfection (adjusted odds ratio = 2.03, 95% confidence interval = 1.14–3.62) after adjusting for age group, body mass index group, and use of proton pump inhibitor.Conclusion. Osteoporosis was found to be associated withH. pyloriinfection in Taiwanese female patients with upper gastrointestinal diseases. Further studies with information on potential confounders are needed to confirm the association.

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