scholarly journals In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Maxime Y. Tondreau ◽  
Véronique Laterreur ◽  
Karine Vallières ◽  
Robert Gauvin ◽  
Jean-Michel Bourget ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Self Assembly ◽  
Three Dimensional ◽  
Small Diameter ◽  
Storage Period ◽  
Extended Period ◽  
Dermal Fibroblasts ◽  
Sprague Dawley ◽  
Tissue Engineered Blood Vessels ◽  
Vascular Scaffolds

There is a clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are halted by the limited suitability of autologous or synthetic grafts. This study uses the self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold (FDVS) that can be available off-the-shelf. Briefly, extracellular matrix scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and decellularized by immersion in deionized water. The FDVSs were implanted as an aortic interpositional graft in six Sprague-Dawley rats for 6 months. Five out of the six implants were still patent 6 months after the surgery. Histological analysis showed the infiltration of cells on both abluminal and luminal sides, and immunofluorescence analysis suggested the formation of neomedia comprised of smooth muscle cells and lined underneath with an endothelium. Furthermore, to verify the feasibility of producing tissue-engineered blood vessels of clinically relevant length and diameter, scaffolds with a 4.6 mm inner diameter and 17 cm in length were fabricated with success and stored for an extended period of time, while maintaining suitable properties following the storage period. This novel demonstration of the potential of the FDVS could accelerate the clinical availability of tissue-engineered blood vessels and warrants further preclinical studies.

Biomechanical Properties of Self-Assembly Tissue Engineered Blood Vessels: Insights Into Assembly Techniques

2010 ◽  
Author(s):  
Michael T. Zaucha ◽  
Rudolph Gleason
Keyword(s):  
Coronary Artery Disease ◽  
Blood Vessels ◽  
Self Assembly ◽  
Small Diameter ◽  
Biomechanical Properties ◽  
Industrialized Nations ◽  
Tissue Engineered Blood Vessels ◽  
Artery Disease ◽  
Thrombogenic Potential ◽  
Synthetic Grafts

Coronary artery disease remains to be the leading cause of morbidity and mortality in industrialized nations. Current treatments for small diameter grafts are limited by the availability of suitable autologous vessels and high thrombogenic potential of synthetic grafts. There is a clinical need to development of tissue engineered blood vessels (TEBV) suitable for vascular by pass grafting.

Theory and Experiments for Mechanically-Induced Remodeling of Tissue Engineered Blood Vessels

2008 ◽  
Vol 57 ◽  
pp. 226-234 ◽  
Author(s):  
Rudolph L. Gleason ◽  
William Wan
Keyword(s):  
Mechanical Properties ◽  
Blood Vessels ◽  
Laser Scanning ◽  
Biomechanical Testing ◽  
Small Diameter ◽  
Mechanical Stimuli ◽  
Two Photon ◽  
Constrained Mixture ◽  
Tissue Engineered Blood Vessels ◽  
Luminal Flow

There is a great unmet clinical need to develop small diameter tissue engineered blood vessels (TEBV) with low thrombogenicity and immune response and suitable mechanical properties. In this paper we describe experimental and computational frameworks to characterize the use of mechanical stimuli to improve the mechanical properties of TEBVs. We model the TEBV as a constrained mixture and track the production, degradation, mechanical state, and organization of each structural constituent. Specifically, we assume that individual load bearing constituents can co-exist within each neighborhood and, although they are constrained to deform together, each constituent within this neighborhood may have different natural (i.e., stress-free) configurations. Motivated by this theoretical framework, we have designed a bioreactor and biomechanical testing device for TEBVs. This device is designed to provide precise and independent control of mean and cyclic luminal flow rate, transmural pressure, and axial load over weeks and months in culture and perform intermittent biaxial biomechanical tests. This device also fits under a two-photon laser scanning microscope for 3-dimenstional imaging of the content and organization of cells and matrix constituents. These data directly support our theoretical model.

Mesenchymal stem cell-based tissue engineering of small-diameter blood vessels

Vascular ◽  
2011 ◽  
Vol 19 (4) ◽  
pp. 206-213 ◽  
Author(s):  
Jian-De Dong ◽  
Jin-Hong Huang ◽  
Feng Gao ◽  
Zhao-Hui Zhu ◽  
Jian Zhang
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Pulsatile Flow ◽  
Ex Vivo ◽  
Flow Direction ◽  
Small Diameter ◽  
Luminal Surface ◽  
Novel Approach ◽  
Tissue Engineered Blood Vessels ◽  
Von Willebrand

The aim of the study was to construct small-diameter vascular grafts using canine mesenchymal stem cells (cMSCs) and a pulsatile flow bioreactor. cMSCs were isolated from canine bone marrow and expanded ex vivo. cMSCs were then seeded onto the luminal surface of decellularized arterial matrices, which were further cultured in a pulsatile flow bioreactor for four days. Immunohistochemical staining and scanning electron microscopy was performed to characterize the tissue-engineered blood vessels. cMSCs were successfully seeded onto the luminal surface of porcine decellularized matrices. After four-day culture in the pulsatile flow bioreactor, the cells were highly elongated and oriented to the flow direction. Immunohistochemistry demonstrated that the cells cultured under pulsatile flow expressed Von Willebrand factor, an endothelial cell marker. In conclusion, cMSCs seeded onto decellularized arterial matrices could differentiate into endothelial lineage after culturing in a pulsatile flow bioreactor, which provides a novel approach for tissue engineering of small-diameter blood vessels.

scholarly journals Biaxial biomechanical properties of self-assembly tissue-engineered blood vessels

2010 ◽  
Vol 8 (55) ◽  
pp. 244-256 ◽  
Author(s):  
Michael T. Zaucha ◽  
Robert Gauvin ◽  
Francois A. Auger ◽  
Lucie Germain ◽  
Rudolph L. Gleason
Keyword(s):  
Experimental Data ◽  
Self Assembly ◽  
Mechanical Response ◽  
Small Diameter ◽  
Biomechanical Properties ◽  
Coefficient Of Determination ◽  
Material Parameters ◽  
Physiological Loading ◽  
Tissue Engineered Blood Vessels ◽  
Graft Success

Along with insights into the potential for graft success, knowledge of biomechanical properties of small diameter tissue-engineered blood vessel (TEBV) will enable designers to tailor the vessels' mechanical response to closer resemble that of native tissue. Composed of two layers that closely mimic the native media and adventitia, a tissue-engineered vascular adventitia (TEVA) is wrapped around a tissue-engineered vascular media (TEVM) to produce a self-assembled tissue-engineered media/adventia (TEVMA). The current study was undertaken to characterize the biaxial biomechanical properties of TEVM, TEVA and TEVMA under physiological pressures as well as characterize the stress-free reference configuration. It was shown that the TEVA had the greatest compliance over the physiological loading range while the TEVM had the lowest compliance. As expected, compliance of the SA-TEBV fell in between with an average compliance of 2.73 MPa −1 . Data were used to identify material parameters for a microstructurally motivated constitutive model. Identified material parameters for the TEVA and TEVM provided a good fit to experimental data with an average coefficient of determination of 0.918 and 0.868, respectively. These material parameters were used to develop a two-layer predictive model for the response of a TEVMA which fit well with experimental data.

scholarly journals 3D PCL/Gelatin/Genipin Nanofiber Sponge as Scaffold for Regenerative Medicine

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2006
Author(s):  
Markus Merk ◽  
Orlando Chirikian ◽  
Christian Adlhart
Keyword(s):  
Tissue Engineering ◽  
Self Assembly ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Engineering Applications ◽  
Sem Images ◽  
Biologically Relevant ◽  
Naturally Occurring

Recent advancements in tissue engineering and material science have radically improved in vitro culturing platforms to more accurately replicate human tissue. However, the transition to clinical relevance has been slow in part due to the lack of biologically compatible/relevant materials. In the present study, we marry the commonly used two-dimensional (2D) technique of electrospinning and a self-assembly process to construct easily reproducible, highly porous, three-dimensional (3D) nanofiber scaffolds for various tissue engineering applications. Specimens from biologically relevant polymers polycaprolactone (PCL) and gelatin were chemically cross-linked using the naturally occurring cross-linker genipin. Potential cytotoxic effects of the scaffolds were analyzed by culturing human dermal fibroblasts (HDF) up to 23 days. The 3D PCL/gelatin/genipin scaffolds produced here resemble the complex nanofibrous architecture found in naturally occurring extracellular matrix (ECM) and exhibit physiologically relevant mechanical properties as well as excellent cell cytocompatibility. Samples cross-linked with 0.5% genipin demonstrated the highest metabolic activity and proliferation rates for HDF. Scanning electron microscopy (SEM) images indicated excellent cell adhesion and the characteristic morphological features of fibroblasts in all tested samples. The three-dimensional (3D) PCL/gelatin/genipin scaffolds produced here show great potential for various 3D tissue-engineering applications such as ex vivo cell culturing platforms, wound healing, or tissue replacement.

scholarly journals A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1581 ◽  
Author(s):  
Yuanyuan Xu ◽  
Yingying Hu ◽  
Changyong Liu ◽  
Hongyi Yao ◽  
Boxun Liu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Structural Integrity ◽  
Vascular System ◽  
Small Diameter ◽  
Dermal Fibroblasts ◽  
Pluronic F127 ◽  
3D Bioprinting ◽  
Novel Strategy ◽  
Blood Vessel Substitutes

In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts–neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology.

Bilayered vascular scaffolds for engineering cellularized small diameter blood vessels

2010 ◽  
Vol 211 (3) ◽  
pp. S144-S145
Author(s):  
Young Min Ju ◽  
Jin San Choi ◽  
Tamer Aboushwareb ◽  
Anthony Atala ◽  
James Yoo ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter ◽  
Vascular Scaffolds

Acellular vascular scaffolds for tissue engineered blood vessels

2006 ◽  
Vol 39 ◽  
pp. S578
Author(s):  
J.L. Berry ◽  
S.K. Yazdani ◽  
G. Amiel ◽  
J.J. Yoo ◽  
A.A. Atala ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Tissue Engineered Blood Vessels ◽  
Vascular Scaffolds
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