scholarly journals A Novel Strategy for Creating Tissue-Engineered Biomimetic Blood Vessels Using 3D Bioprinting Technology

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1581 ◽  
Author(s):  
Yuanyuan Xu ◽  
Yingying Hu ◽  
Changyong Liu ◽  
Hongyi Yao ◽  
Boxun Liu ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Structural Integrity ◽  
Vascular System ◽  
Small Diameter ◽  
Dermal Fibroblasts ◽  
Pluronic F127 ◽  
3D Bioprinting ◽  
Novel Strategy ◽  
Blood Vessel Substitutes

In this work, a novel strategy was developed to fabricate prevascularized cell-layer blood vessels in thick tissues and small-diameter blood vessel substitutes using three-dimensional (3D) bioprinting technology. These thick vascularized tissues were comprised of cells, a decellularized extracellular matrix (dECM), and a vasculature of multilevel sizes and multibranch architectures. Pluronic F127 (PF 127) was used as a sacrificial material for the formation of the vasculature through a multi-nozzle 3D bioprinting system. After printing, Pluronic F127 was removed to obtain multilevel hollow channels for the attachment of human umbilical vein endothelial cells (HUVECs). To reconstruct functional small-diameter blood vessel substitutes, a supporting scaffold (SE1700) with a double-layer circular structure was first bioprinted. Human aortic vascular smooth muscle cells (HA-VSMCs), HUVECs, and human dermal fibroblasts–neonatal (HDF-n) were separately used to form the media, intima, and adventitia through perfusion into the corresponding location of the supporting scaffold. In particular, the dECM was used as the matrix of the small-diameter blood vessel substitutes. After culture in vitro for 48 h, fluorescent images revealed that cells maintained their viability and that the samples maintained structural integrity. In addition, we analyzed the mechanical properties of the printed scaffold and found that its elastic modulus approximated that of the natural aorta. These findings demonstrate the feasibility of fabricating different kinds of vessels to imitate the structure and function of the human vascular system using 3D bioprinting technology.

scholarly journals In VivoRemodeling of Fibroblast-Derived Vascular Scaffolds Implanted for 6 Months in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Maxime Y. Tondreau ◽  
Véronique Laterreur ◽  
Karine Vallières ◽  
Robert Gauvin ◽  
Jean-Michel Bourget ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Self Assembly ◽  
Three Dimensional ◽  
Small Diameter ◽  
Storage Period ◽  
Extended Period ◽  
Dermal Fibroblasts ◽  
Sprague Dawley ◽  
Tissue Engineered Blood Vessels ◽  
Vascular Scaffolds

There is a clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are halted by the limited suitability of autologous or synthetic grafts. This study uses the self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold (FDVS) that can be available off-the-shelf. Briefly, extracellular matrix scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and decellularized by immersion in deionized water. The FDVSs were implanted as an aortic interpositional graft in six Sprague-Dawley rats for 6 months. Five out of the six implants were still patent 6 months after the surgery. Histological analysis showed the infiltration of cells on both abluminal and luminal sides, and immunofluorescence analysis suggested the formation of neomedia comprised of smooth muscle cells and lined underneath with an endothelium. Furthermore, to verify the feasibility of producing tissue-engineered blood vessels of clinically relevant length and diameter, scaffolds with a 4.6 mm inner diameter and 17 cm in length were fabricated with success and stored for an extended period of time, while maintaining suitable properties following the storage period. This novel demonstration of the potential of the FDVS could accelerate the clinical availability of tissue-engineered blood vessels and warrants further preclinical studies.

scholarly journals Three-dimensional printing of cell-laden bioink for blood vessel tissue engineering: Influence of process parameters and components on cell viability

2021 ◽  
Author(s):  
CONGCONG ZHAN ◽  
Yasong Hu ◽  
ANDUO ZHOU ◽  
SHANFENG ZHANG ◽  
Xia Huang
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Cell Viability ◽  
Process Parameters ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Small Diameter ◽  
3D Bioprinting ◽  
Influence Of Process Parameters ◽  
Composite Gels

Three-dimensional (3D) bioprinting is a potential therapeutic method for tissue engineering owing to its ability to prepare cell-laden tissue constructs. The properties of bioink are crucial to accurately control the printing structure. Meanwhile, the effect of process parameters on the precise structure is not nonsignificant. We investigated the correlation between process parameters of 3D bioprinting and the structural response of κ-carrageenan-based hydrogels to explore the controllable structure, printing resolution, and cell survival rate. Small-diameter (<6 mm) gel filaments with different structures were printed by varying the shear stress of the extrusion bioprinter to simulate the natural blood vessel structure. The cell viability of the scaffold was evaluated. The in vitro culture of human umbilical vein endothelium cells (HUVECs) on the κ-carrageenan (kc) and composite gels (carrageenan/carbon nanotube and carrageenan/sodium alginate) demonstrated that the cell attachment and proliferation on composite gels were better than those on pure kc. Our results revealed that the carrageenan-based composite bioinks offer better printability, sufficient mechanical stiffness, interconnectivity, and biocompatibility. This process can facilitate precise adjustment of the pore size, porosity, and pore distribution of the hydrogel structure by optimising the printing parameters as well as realise the precise preparation of the internal structure of the 3D hydrogel-based tissue engineering scaffold. Moreover, we obtained perfused tubular filament by 3D printing at optimal process parameters.

scholarly journals Photooxidation crosslinking to recover residual stress in decellularized blood vessel

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Jintao Wang ◽  
Lingwen Kong ◽  
Alidha Gafur ◽  
Xiaobo Peng ◽  
Natalia Kristi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Residual Stress ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Opening Angle ◽  
Elastic Fibers ◽  
Transmission Electron ◽  
Small Diameter Vascular Grafts

Abstract Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts. However, this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress. Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts. To improve the situation, acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively, and the changes of opening angle, circumferential residual strain of native blood vessels, decellularized arteries and crosslinked blood vessels were measured by means of histological examination, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) in this study. The opening angle of decellularized arteries significantly restored after photooxidation crosslinking (P = 0.0216), while that of glutaraldehyde crosslinking blood vessels reduced. The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking. The results of finite element simulation showed that the residual stress increased with the increase of opening angle. In this study, we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels, which provides biomechanical support for the development of new biomaterials of vascular grafts.

3D Bioprinting-Tunable Small-Diameter Blood Vessels with Biomimetic Biphasic Cell Layers

2020 ◽  
Vol 12 (41) ◽  
pp. 45904-45915
Author(s):  
Xuan Zhou ◽  
Margaret Nowicki ◽  
Hao Sun ◽  
Sung Yun Hann ◽  
Haitao Cui ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter ◽  
3D Bioprinting ◽  
Cell Layers

Fabrication of PCL/keratin composite scaffolds for vascular tissue engineering with catalytic generation of nitric oxide potential

2020 ◽  
Vol 8 (28) ◽  
pp. 6092-6099
Author(s):  
Pengfei Li ◽  
Yanfang Wang ◽  
Xingxing Jin ◽  
Jie Dou ◽  
Xiao Han ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tissue Engineering ◽  
Blood Vessel ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Composite Scaffolds ◽  
Promising Solution ◽  
Blood Vessel Substitutes

Tissue-engineered vascular grafts (TEVGs) have been proposed as a promising solution to fulfill the need for small-diameter blood vessel substitutes.

COMPARISON OF ANTIHYPERTENSIVE EFFECT OF MOXONIDINE VERSUS CLONIDINE IN RENAL FAILURE PATIENTS.

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
DR.MATHEW GEORGE ◽  
DR.LINCY JOSEPH ◽  
MRS.DEEPTHI MATHEW ◽  
ALISHA MARIA SHAJI ◽  
BIJI JOSEPH ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Renal Failure ◽  
Blood Flow ◽  
Blood Vessel ◽  
Blood Vessels ◽  
High Blood Pressure ◽  
Antihypertensive Effect ◽  
The Body ◽  
Ability To Work ◽  
Blood Flows

Blood pressure is the force of blood pushing against blood vessel walls as the heart pumps out blood, and high blood pressure, also called hypertension, is an increase in the amount of force that blood places on blood vessels as it moves through the body. Factors that can increase this force include higher blood volume due to extra fluid in the blood and blood vessels that are narrow, stiff, or clogged(1). High blood pressure can damage blood vessels in the kidneys, reducing their ability to work properly. When the force of blood flow is high, blood vessels stretch so blood flows more easily. Eventually, this stretching scars and weakens blood vessels throughout the body, including those in the kidneys.

Microfluidics of blood in blood vessels stenosis

2016 ◽  
Vol 11 (2) ◽  
pp. 210-217 ◽  
Author(s):  
A.T. Akhmetov ◽  
A.A. Valiev ◽  
A.A. Rakhimov ◽  
S.P. Sametov ◽  
R.R. Habibullina
Keyword(s):  
Blood Flow ◽  
Blood Vessels ◽  
Hydraulic Resistance ◽  
Microfluidic Device ◽  
Human Body ◽  
Vascular System ◽  
Hydrodynamic Conditions ◽  
Microstructure Change ◽  
Healthy Part

It is mentioned in the paper that hydrodynamic conditions of a flow in blood vessels with the stenosis are abnormal in relation to the total hemodynamic conditions of blood flow in a vascular system of a human body. A microfluidic device developed with a stepped narrowing for studying of the blood flow at abnormal conditions allowed to reveal blood structure in microchannels simulating the stenosis. Microstructure change is observed during the flow of both native and diluted blood through the narrowing. The study of hemorheological properties allowed us to determine an increasing contribution of the hydraulic resistance of the healthy part of the vessel during the stenosis formation.

scholarly journals Retinal Vessel Segmentation by Deep Residual Learning with Wide Activation

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Yuliang Ma ◽  
Xue Li ◽  
Xiaopeng Duan ◽  
Yun Peng ◽  
Yingchun Zhang
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Area Under The Curve ◽  
Retinal Vessel ◽  
Vessel Segmentation ◽  
Superior Performance ◽  
Retinal Blood Vessel ◽  
Low Contrast ◽  
Blood Vessel Segmentation ◽  
Small Vessels

Purpose. Retinal blood vessel image segmentation is an important step in ophthalmological analysis. However, it is difficult to segment small vessels accurately because of low contrast and complex feature information of blood vessels. The objective of this study is to develop an improved retinal blood vessel segmentation structure (WA-Net) to overcome these challenges. Methods. This paper mainly focuses on the width of deep learning. The channels of the ResNet block were broadened to propagate more low-level features, and the identity mapping pathway was slimmed to maintain parameter complexity. A residual atrous spatial pyramid module was used to capture the retinal vessels at various scales. We applied weight normalization to eliminate the impacts of the mini-batch and improve segmentation accuracy. The experiments were performed on the DRIVE and STARE datasets. To show the generalizability of WA-Net, we performed cross-training between datasets. Results. The global accuracy and specificity within datasets were 95.66% and 96.45% and 98.13% and 98.71%, respectively. The accuracy and area under the curve of the interdataset diverged only by 1%∼2% compared with the performance of the corresponding intradataset. Conclusion. All the results show that WA-Net extracts more detailed blood vessels and shows superior performance on retinal blood vessel segmentation tasks.

scholarly journals A Near‐Infrared Organic Fluorescent Probe for Broad Applications for Blood Vessels Imaging by High‐Throughput Screening via 3D‐Blood Vessel Models (Small Methods 8/2021)

Small Methods ◽  
2021 ◽  
Vol 5 (8) ◽  
pp. 2170036
Author(s):  
Muhammad Asri Abdul Sisak ◽  
Fiona Louis ◽  
Ichio Aoki ◽  
Sun Hyeok Lee ◽  
Young‐Tae Chang ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Fluorescent Probe ◽  
High Throughput ◽  
High Throughput Screening ◽  
Near Infrared
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