scholarly journals Preparation and Characterization of a Collagen-Liposome-Chondroitin Sulfate Matrix with Potential Application for Inflammatory Disorders Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Oana Craciunescu ◽  
Alexandra Gaspar ◽  
Mihaela Trif ◽  
Magdalena Moisei ◽  
Anca Oancea ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Cell Metabolism ◽  
Local Treatment ◽  
Fibroblast Cell ◽  
Specific Staining ◽  
Inflammatory Disorders ◽  
Cell Matrix ◽  
L929 Fibroblast ◽  
The Matrix

Smart drug delivery systems with controllable properties play an important role in targeted therapy and tissue regeneration. The aim of our study was the preparation andin vitroevaluation of a collagen (Col) matrix embedding a liposomal formulation of chondroitin sulfate (L-CS) for the treatment of inflammatory disorders. Structural studies using Oil Red O specific staining for lipids and scanning electron microscopy showed an alveolar network of nanosized Col fibrils decorated with deposits of L-CS at both periphery and inner of the matrix. The porosity and density of Col-L-CS matrix were similar to those of Col matrix, while its mean pore size and biodegradability had significantly higher and lower values (P<0.05), respectively.In vitrocytotoxicity assays showed that the matrix system induced high cell viability and stimulated cell metabolism in L929 fibroblast cell culture. Light and electron micrographs of the cell-matrix construct showed that cells clustered into the porous structure at 72 h of cultivation.In vitrodiffusion test indicated that the quantity of released CS was significantly lower (P<0.05) after embedment of L-CS within Col matrix. All these results indicated that the biocompatible and biodegradable Col-L-CS matrix might be a promising delivery system for local treatment of inflamed site.

scholarly journals INCREASED FIXATION OF SULFUR35 BY CARTILAGE IN VITRO FOLLOWING DEPLETION OF THE MATRIX BY INTRAVENOUS PAPAIN

1960 ◽  
Vol 112 (5) ◽  
pp. 743-750 ◽  
Author(s):  
T. F. McElligott ◽  
J. L. Potter
Keyword(s):  
Articular Cartilage ◽  
Chondroitin Sulfate ◽  
Intravenous Injection ◽  
Experimental Situation ◽  
Costal Cartilage ◽  
Primary Lesion ◽  
Cartilage Matrix ◽  
Osteoarthritic Cartilage ◽  
The Matrix

The uptake in vitro of sulfur-35 by costal cartilage obtained from nine rabbits 11 days after an intravenous injection of crude papain solution was compared with that in costal cartilage from eight normal untreated rabbits. An increased fixation of the isotope was found in treated animals compared with controls. The depletion of cartilage matrix by papain provided an experimental situation to test the hypothesis that the depletion of matrix which occurs in osteoarthritic cartilage can stimulate increased synthesis of chondroitin sulfate. The results give further support to the view that the primary lesion in osteoarthritis occurs in the matrix rather than in the chondrocyte of articular cartilage.

Characterization and In Vitro Toxicity of French Process Zinc Oxide Nanoparticles with High Surficial Zinc

2019 ◽  
Vol 290 ◽  
pp. 274-279
Author(s):  
Sufiniza Nordin ◽  
Shahrom Mahmud ◽  
Azman Seeni ◽  
Nur Mariam Kamaruddin ◽  
Nur Syuhada Ahmad
Keyword(s):  
L929 Cell ◽  
Average Crystallite Size ◽  
Fibroblast Cell ◽  
In Vitro Toxicity ◽  
Visible Absorption ◽  
Zno Nanopowder ◽  
L929 Fibroblast ◽  
Calculated Average ◽  
L929 Cell Line

In this study, we investigated in vitro toxicity of ZnO nanopowder on L929 fibroblast cell lines. The ZnO nanoparticles were observed to possess relatively more surficial zinc compared to oxygen. Field-emission scanning electron microscope (FESEM) data revealed that the particle morphologies consisted of nanorods, platelets and nodules between 40-100 nm size range. EDS confirmed that there were more zinc elements on the surfaces of the particles. XRD results showed that the calculated average crystallite size of ZnO nanopowder was 44.28 nm. The optical band gap calculated was 3.298 eV based on UV-visible absorption spectra. In vitro toxicity results showed that ZnO concentration at 0.3125mM, 0.625mM and 1.25 mM were considered non-toxic to L929 cell line since the cell viability was higher than 70 % after 72 hours treatment whereas the ZnO nanopowder concentration above 2.5mM was considered toxic. High surficial zinc atoms on ZnO particles could have been a significant factor in cell toxicity.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (09) ◽  
pp. 540-555
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B ◽  
Dinesh, MD ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

The matrix secreted by 804G cells contains laminin-related components that participate in hemidesmosome assembly in vitro

1993 ◽  
Vol 105 (3) ◽  
pp. 753-764 ◽  
Author(s):  
M. Langhofer ◽  
S.B. Hopkinson ◽  
J.C. Jones
Keyword(s):  
Lectin Binding ◽  
Rabbit Antiserum ◽  
Epithelial Cell Line ◽  
Antigenic Relationship ◽  
Matrix Elements ◽  
Cell Matrix ◽  
The Matrix ◽  
Bona Fide ◽  
Functional Analyses

Hemidesmosomes are important adhesion devices found in epithelial cells. They connect the intermediate filament cytoskeleton network with components of the basement membrane zone. 804G cells are an unusual epithelial cell line, since they form bona fide hemidesmosomes when plated on glass or plastic. In this study we tested an hypothesis: that this ability is a consequence of an extracellular component produced by the 804G cells. As probes for our study we generated a rabbit antiserum (J18) and monoclonal antibodies against components of urea-solubilized 804G matrix. Antibodies in the J18 serum recognize major lectin-binding polypeptides of 150, 140 and 135 kDa in the 804G matrix. A monoclonal antibody (5C5) that shows reactivity with the 150 and 135 kDa polypeptides in western immunoblots immunoprecipitates all three molecular mass species, indicating that these polypeptides are part of a matrix complex. Moreover, one, at least, of these matrix elements is immunologically related to laminin, since J18 antibodies selected on fusion protein fragments of a newly characterized laminin variant, laminin B2t (Kallunki et al., J. Cell Biol., 119, 679–694, 1992), react with the 140 kDa polypeptide component of the 804G cell matrix. To undertake functional analyses of 804G matrix, cells of the human epidermal carcinoma line SCC12, which do not assemble bona fide hemidesmosomes in vitro, were cultured on 804G matrix for 24 h and then analysed by confocal immunofluorescence and electron microscopy. In SCC12 cells maintained on 804G cell matrix, hemidesmosomal antigens localize in a distinctive leopard spot pattern that mirrors the distribution of 804G matrix elements. Furthermore, ultrastructural analysis reveals that the 804G cell matrix supports the formation of ‘mature’ hemidesmosomes by SCC12 cells. Thus 804G cell matrix is a remarkable tool for hemidesmosome studies and it will now be of great importance to determine the exact composition of the 804G matrix, especially its structural and antigenic relationship to laminins.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (10) ◽  
pp. 206-221
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B, ◽  
Uma, C ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

Morphological and autoradiographic studies of cleft palate induced in rat embryos by maternal hypervitaminosis A

Development ◽  
1965 ◽  
Vol 14 (3) ◽  
pp. 223-238
Author(s):  
Devendra M. Kochhar ◽  
E. Marshall Johnson
Keyword(s):  
Cleft Palate ◽  
Chondroitin Sulfate ◽  
Congenital Malformations ◽  
Hypervitaminosis A ◽  
Rat Embryos ◽  
Controlled Treatment ◽  
High Incidence ◽  
The Matrix

In the laboratory many teratogenic procedures have been found to produce cleft palate in conjunction with other congenital malformations (Kalter & Warkany, 1959), but only in embryos of cortisone treated mice has this malformation been reported to occur singly (Baxter & Fraser, 1950). However, a carefully controlled treatment with hypervitaminosis A can produce a high incidence of cleft palate which is only rarely accompanied by other malformations (Giroud & Martinet, 1956). These two teratogenic treatments have other similarities in that both similarly affect the ultimate chemical composition of tissues. That is, both in vivo and in vitro, cortisone depresses the ability of tissues to synthesize chondroitin sulfate (Layton, 1951a, 1951b; Schiller & Dorfman, 1957) and, in animals receiving excessive doses of vitamin A, chondroitin sulfate was removed from the matrix of epiphyseal and articular cartilages (Thomas et al., 1960).

IN VITRO CYTOCOMPATIBILITY OF SYNTHETIC CALCIUM PHOSPHATE POWDER ON L929 FIBROBLAST CELL

2015 ◽  
Vol 23 (1) ◽  
pp. 15-24
Author(s):  
Shirin Ibrahim ◽  
◽  
MUHAMAD ANAS MARZUKE ◽  
ZUL HAZMI HUSSIN ◽  
NOR SHAHIDA KADER BASHAH ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Fibroblast Cell ◽  
Phosphate Powder ◽  
L929 Fibroblast ◽  
Synthetic Calcium Phosphate

Variants of integrin β4 subunit in human endometrial adenocarcinoma cells: mediators of ECM-induced differentiation?

1996 ◽  
Vol 74 (6) ◽  
pp. 867-873 ◽  
Author(s):  
Elisabeth Strunck ◽  
Gunter Vollmer
Keyword(s):  
Extracellular Matrix ◽  
Endometrial Adenocarcinoma ◽  
Tumor Development ◽  
Functional Differentiation ◽  
Cell Matrix ◽  
Integrin Β4 ◽  
The Matrix ◽  
And Function ◽  
Cell Matrix Interactions

The influence of extracellular matrix (ECM) on expression and function of integrins in carcinogenesis and differentiation is not well understood, but the importance of altered adhesion features for tumor development and progression is obvious. Integrins as versatile molecules are mainly responsible for mediating cell–matrix interactions and transmembrane signal transduction. They are capable of transducing outside-in signals from ECM components or conversely to organize the matrix by inside-out signaling. In the study presented here, we report that the reconstituted basement membrane, Matrigel™, which induces morphological and functional differentiation of the endometrial adenocarcinoma cell line HEC 1B(L), also regulates the expression of various forms of the integrin β4 subunit. Furthermore, we were able to identify full-length isoforms with and without an altered cytoplasmic domain as well as truncated forms. Our findings suggest a regulatory role of integrin β4 isoforms and fragments in the process of in vitro differentiation of HEC 1B(L).Key words: endometrium, tumor cells, differentiation, extracellular matrix, β4-integrin expression.

scholarly journals Evaluation of Cytotoxicity of Diclofenac Sodium on L929 Fibroblasts - An in vitro Study

2020 ◽  
pp. 171-177
Author(s):  
Mulumoodi Rama Sowmya ◽  
P. Ajitha ◽  
S. Pradeep
Keyword(s):  
Calcium Hydroxide ◽  
Diclofenac Sodium ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Antimicrobial Efficacy ◽  
Control Group ◽  
Significant Difference ◽  
L929 Fibroblast ◽  
Diphenyl Tetrazolium Bromide

The aim of the study is to evaluate comparatively the cytotoxicity of diclofenac sodium and calcium hydroxide on L929 fibroblasts. L929 fibroblast cells were cultured and grown on Dulbecco modified Eagle’s medium. Intracanal medicaments tested were Diclofenac sodium, 5.0, 7.5, 10.0 mM/ml) and calcium hydroxide. The human fibroblast cell lines cultured in Dulbecco Modified Eagle’s medium were used as control group. Cytotoxicity was evaluated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results showed that there was a significant difference in cell viability as compared with the control group (P<0.05). There was no significant difference in the group treated with diclofenac sodium and calcium hydroxide (1.0 mM/ml). However, diclofenac sodium at concentration more than 5 mM/ml was found to be cytotoxic. The study concludes that diclofenac sodium is cytotoxic at 5 mM/ml and above. Therefore, further studies are recommended to establish the antimicrobial efficacy of the medicament. Within the limitations of the study, Diclofenac sodium at concentration more than 5mM/ml was found to be cytotoxic for the cells. The inhibitory concentration (IC50) of Diclofenac sodium at which the cells were viable was found to be 5.2 mM/ml. Further studies should be done to establish the antimicrobial efficacy of the medicament at these concentrations.

scholarly journals Macromolecular properties that promote mesangial binding and mesangiopathic nephritis.

1992 ◽  
Vol 2 (10) ◽  
pp. S149 ◽  
Author(s):  
S N Emancipator ◽  
C S Rao ◽  
A Amore ◽  
R Coppo ◽  
J G Nedrud
Keyword(s):  
Cell Surface ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Sendai Virus ◽  
Cell Metabolism ◽  
Immunoglobulin A ◽  
Cross Linking ◽  
The Matrix

The hydrodynamic size, electrostatic charge, and specificity are established determinants of the site of glomerular localization of macromolecules. Larger macromolecules or aggregates and anionic charge are associated with mesangial deposits, despite the fact that the mesangial matrix bears a negative charge similar to that of the capillary wall. Antigens such as Sendai virus, a model infectious pathogen, gliadin, a model dietary/environmental agent and fibronectin, a model endogenous macromolecule, bind to mesangial cells in vitro on the basis of cell surface glycoconjugates. Nonantibody immunoglobulin A, which does not bind to cells directly, binds to these elements via different carbohydrate specificities (simple sugar inhibition). Such binding promotes or augments macromolecular deposition in the mesangium. More significantly, mesangial deposits per se are not pathogenic, because normal renal function can be observed with florid deposits. Pathogenic deposits must have properties that alter mesangial cell metabolism or interaction with the matrix. Although complement activation is well recognized, complement-independent mechanisms related to cell surface modulation are being recognized. In vitro, antigen/immunoglobulin A aggregates alter mesangial cell eicosanoid synthesis. In vivo, large-lattice cross-linking by particulate antigen promotes hematuria. We conclude that the binding of macromolecules to cells and the cross-linking of cell surface molecules cause alterations in the mesangial cells and therefore in glomerular function. The mesangial cell, rather than a passive respondent, is an active participant in the genesis of glomerulonephritis.

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