IN VITRO CYTOCOMPATIBILITY OF SYNTHETIC CALCIUM PHOSPHATE POWDER ON L929 FIBROBLAST CELL

2015 ◽  
Vol 23 (1) ◽  
pp. 15-24
Author(s):  
Shirin Ibrahim ◽  
◽  
MUHAMAD ANAS MARZUKE ◽  
ZUL HAZMI HUSSIN ◽  
NOR SHAHIDA KADER BASHAH ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Fibroblast Cell ◽  
Phosphate Powder ◽  
L929 Fibroblast ◽  
Synthetic Calcium Phosphate

Characterization and In Vitro Toxicity of French Process Zinc Oxide Nanoparticles with High Surficial Zinc

2019 ◽  
Vol 290 ◽  
pp. 274-279
Author(s):  
Sufiniza Nordin ◽  
Shahrom Mahmud ◽  
Azman Seeni ◽  
Nur Mariam Kamaruddin ◽  
Nur Syuhada Ahmad
Keyword(s):  
L929 Cell ◽  
Average Crystallite Size ◽  
Fibroblast Cell ◽  
In Vitro Toxicity ◽  
Visible Absorption ◽  
Zno Nanopowder ◽  
L929 Fibroblast ◽  
Calculated Average ◽  
L929 Cell Line

In this study, we investigated in vitro toxicity of ZnO nanopowder on L929 fibroblast cell lines. The ZnO nanoparticles were observed to possess relatively more surficial zinc compared to oxygen. Field-emission scanning electron microscope (FESEM) data revealed that the particle morphologies consisted of nanorods, platelets and nodules between 40-100 nm size range. EDS confirmed that there were more zinc elements on the surfaces of the particles. XRD results showed that the calculated average crystallite size of ZnO nanopowder was 44.28 nm. The optical band gap calculated was 3.298 eV based on UV-visible absorption spectra. In vitro toxicity results showed that ZnO concentration at 0.3125mM, 0.625mM and 1.25 mM were considered non-toxic to L929 cell line since the cell viability was higher than 70 % after 72 hours treatment whereas the ZnO nanopowder concentration above 2.5mM was considered toxic. High surficial zinc atoms on ZnO particles could have been a significant factor in cell toxicity.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (09) ◽  
pp. 540-555
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B ◽  
Dinesh, MD ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

scholarly journals PREPARATION AND IN VITRO TEST OF APATITE FILMS ONTO TITANIUM BY SPUTTERING FROM CALCIUM PHOSPHATE POWDER TARGETS

1999 ◽  
Vol 10 (0) ◽  
pp. 370-374
Author(s):  
JUN-ICHI HAMAGAMI ◽  
DAISUKE KOKUBU ◽  
KIYOSHI KANAMURA ◽  
TAKAO UMEGAKI ◽  
KIMIHIRO YAMASHITA
Keyword(s):  
Calcium Phosphate ◽  
In Vitro Test ◽  
Phosphate Powder ◽  
Vitro Test

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (10) ◽  
pp. 206-221
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B, ◽  
Uma, C ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

Assay of in vitro osteoclast activity on dentine, and synthetic calcium phosphate bone substitutes

2011 ◽  
Vol 23 (3) ◽  
pp. 797-803 ◽  
Author(s):  
Zahi Badran ◽  
Paul Pilet ◽  
Elise Verron ◽  
Jean-Michel Bouler ◽  
Pierre Weiss ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Substitutes ◽  
Osteoclast Activity ◽  
Synthetic Calcium Phosphate

scholarly journals Evaluation of Cytotoxicity of Diclofenac Sodium on L929 Fibroblasts - An in vitro Study

2020 ◽  
pp. 171-177
Author(s):  
Mulumoodi Rama Sowmya ◽  
P. Ajitha ◽  
S. Pradeep
Keyword(s):  
Calcium Hydroxide ◽  
Diclofenac Sodium ◽  
In Vitro Study ◽  
Fibroblast Cell ◽  
Antimicrobial Efficacy ◽  
Control Group ◽  
Significant Difference ◽  
L929 Fibroblast ◽  
Diphenyl Tetrazolium Bromide

The aim of the study is to evaluate comparatively the cytotoxicity of diclofenac sodium and calcium hydroxide on L929 fibroblasts. L929 fibroblast cells were cultured and grown on Dulbecco modified Eagle’s medium. Intracanal medicaments tested were Diclofenac sodium, 5.0, 7.5, 10.0 mM/ml) and calcium hydroxide. The human fibroblast cell lines cultured in Dulbecco Modified Eagle’s medium were used as control group. Cytotoxicity was evaluated by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The results showed that there was a significant difference in cell viability as compared with the control group (P<0.05). There was no significant difference in the group treated with diclofenac sodium and calcium hydroxide (1.0 mM/ml). However, diclofenac sodium at concentration more than 5 mM/ml was found to be cytotoxic. The study concludes that diclofenac sodium is cytotoxic at 5 mM/ml and above. Therefore, further studies are recommended to establish the antimicrobial efficacy of the medicament. Within the limitations of the study, Diclofenac sodium at concentration more than 5mM/ml was found to be cytotoxic for the cells. The inhibitory concentration (IC50) of Diclofenac sodium at which the cells were viable was found to be 5.2 mM/ml. Further studies should be done to establish the antimicrobial efficacy of the medicament at these concentrations.

scholarly journals Preparation and Characterization of a Collagen-Liposome-Chondroitin Sulfate Matrix with Potential Application for Inflammatory Disorders Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Oana Craciunescu ◽  
Alexandra Gaspar ◽  
Mihaela Trif ◽  
Magdalena Moisei ◽  
Anca Oancea ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Cell Metabolism ◽  
Local Treatment ◽  
Fibroblast Cell ◽  
Specific Staining ◽  
Inflammatory Disorders ◽  
Cell Matrix ◽  
L929 Fibroblast ◽  
The Matrix

Smart drug delivery systems with controllable properties play an important role in targeted therapy and tissue regeneration. The aim of our study was the preparation andin vitroevaluation of a collagen (Col) matrix embedding a liposomal formulation of chondroitin sulfate (L-CS) for the treatment of inflammatory disorders. Structural studies using Oil Red O specific staining for lipids and scanning electron microscopy showed an alveolar network of nanosized Col fibrils decorated with deposits of L-CS at both periphery and inner of the matrix. The porosity and density of Col-L-CS matrix were similar to those of Col matrix, while its mean pore size and biodegradability had significantly higher and lower values (P<0.05), respectively.In vitrocytotoxicity assays showed that the matrix system induced high cell viability and stimulated cell metabolism in L929 fibroblast cell culture. Light and electron micrographs of the cell-matrix construct showed that cells clustered into the porous structure at 72 h of cultivation.In vitrodiffusion test indicated that the quantity of released CS was significantly lower (P<0.05) after embedment of L-CS within Col matrix. All these results indicated that the biocompatible and biodegradable Col-L-CS matrix might be a promising delivery system for local treatment of inflamed site.

Design, Synthesis, In vitro and In silico Evaluation of New Hydrazonebased Antitumor Agents as Potent Akt Inhibitors

2020 ◽  
Vol 17 (11) ◽  
pp. 1380-1392
Author(s):  
Emine Merve Güngör ◽  
Mehlika Dilek Altıntop ◽  
Belgin Sever ◽  
Gülşen Akalın Çiftçi
Keyword(s):  
Cell Line ◽  
Anticancer Agents ◽  
In Silico ◽  
Antitumor Agents ◽  
Colorimetric Assay ◽  
Fibroblast Cell ◽  
Lipinski’S Rule ◽  
In Silico Docking ◽  
Embryonic Fibroblast

Background: Akt is overexpressed or activated in a variety of human cancers, including gliomas, lung, breast, ovarian, gastric and pancreatic carcinomas. Akt inhibition leads to the induction of apoptosis and inhibition of tumor growth and therefore extensive efforts have been devoted to the discovery of potent antitumor drugs targeting Akt. Objectives: The objective of this work was to identify potent anticancer agents targeting Akt. Methods: New hydrazone derivatives were synthesized and investigated for their cytotoxic effects on 5RP7 H-ras oncogene transformed rat embryonic fibroblast and L929 mouse embryonic fibroblast cell lines. Besides, the apoptotic effects of the most active compounds on 5RP7 cell line were evaluated using flow cytometry. Their Akt inhibitory effects were also investigated using a colorimetric assay. In silico docking and Absorption, Distribution, Metabolism and Excretion (ADME) studies were also performed using Schrödinger’s Maestro molecular modeling package. Results and Discussion: Compounds 3a, 3d, 3g and 3j were found to be effective on 5RP7 cells (with IC50 values of <0.97, <0.97, 1.13±0.06 and <0.97 μg/mL, respectively) when compared with cisplatin (IC50= 1.87±0.15 μg/mL). It was determined that these four compounds significantly induced apoptosis in 5RP7 cell line. Among them, N'-benzylidene-2-[(4-(4-methoxyphenyl)pyrimidin- 2-yl)thio]acetohydrazide (3g) significantly inhibited Akt (IC50= 0.5±0.08 μg/mL) when compared with GSK690693 (IC50= 0.6±0.05 μg/mL). Docking studies suggested that compound 3g showed good affinity to the active site of Akt (PDB code: 2JDO). According to in silico ADME studies, the compound also complies with Lipinski's rule of five and Jorgensen's rule of three. Conclusion: Compound 3g stands out as a potential orally bioavailable cytotoxic agent and apoptosis inducer targeting Akt.

New Ferrocene Compounds as Selective Cyclooxygenase (COX-2) Inhibitors: Design, Synthesis, Cytotoxicity and Enzyme-inhibitory Activity

2018 ◽  
Vol 18 (2) ◽  
pp. 295-301 ◽  
Author(s):  
Shabnam Farzaneh ◽  
Elnaz Zeinalzadeh ◽  
Bahram Daraei ◽  
Soraya Shahhosseini ◽  
Afshin Zarghi
Keyword(s):  
Cell Lines ◽  
Inhibitory Activity ◽  
Fibroblast Cell ◽  
Breast Cancer Cell Lines ◽  
Ferrocene Derivatives ◽  
Cox 2 ◽  
Cytotoxicity Effects ◽  
Cox 2 Inhibitors ◽  
Mcf 7

Background: Due to the astonishing properties of ferrocene and its derivatives, it has a broad application in diverse areas. Numerous ferrocene derivatives demonstrated anti-proliferative activity. Also COX-2, as a key isoenzyme for production of prostaglandins, is frequently overexpressed in various cancers. It is now recognized that COX-2 over expression promotes tumorigenic functions which can be suppressed by COX-2 inhibitors, a phenomenon useful for the preventing of tumor progression. The combination of COX-2 inhibitors with other anti-cancer or cancer prevention drugs may reduce their side effects in future cancer prevention and treatment. Objective: Owing to high anticancer potential of ferrocene derivatives and considerable COX-2 inhibitory and cytotoxicity effects of our previously synthesized chalcones, we decided to incorporate the ferrocenyl moiety into appropriate COX-2 inhibitor chalcone based scaffold, to evaluate COX-2 inhibitory activity as well as anticancer activities. Methods: Chalcones were synthesized via clasien-schmidt condensation of methylsulfonyl aldehyde and acetyl ferrocene. Further different amines with solvent free and ultra sound condition were reacted with chalcones to have different 1-ferrocenyl-3-amino carbonyl compounds. Docking study was carried out with Auto Dock vina software. All the newly-synthesized compounds were evaluated for their cyclooxygenase-2 (COX-2) inhibitory activity using chemiluminescent enzyme assays as well as cytotoxicity activity against MCF-7 and T47D and fibroblast cell lines by MTT assay. Results: In vitro COX-1/COX-2 inhibition studies demonstrated that all compounds were selective inhibitors of the COX-2 isozyme with IC50 values in the highly potent 0.05-0.12 µM range, and COX-2 selectivity indexes (SI) in the 148.3-313.7 range. These results indicated that either potency or selectivity of COX-2 inhibitory activity was affected by the nature and size of the substituents on C-3 of propane-1-one. Also anti-proliferative and toxicity activities of synthesized compounds against breast cancer cell lines MCF-7 and T47D and fibroblast cell lines showed that the synthesized compounds had mild to moderate cytotoxicity against MCT7 and T47D breast cancer cell lines at 10 µM concentration. In vitro COX-1/COX-2 inhibition studies and anticancer activity against MCF-7, identified 1-ferrocenyl-3-(4-methylsulfonylphenyl) propen-1-one as a potent compound (IC50 COX-2 = 0.05 µM, MCF-7: % inhibition (at concentration of 10 µM) = 32.7%), and also 1-ferrocenyl-3- (propan-1-amine)-3-(4-methylsulfonylphenyl) propan-1-one showed the most selectivity on COX-2 inhibition (selectivity index= 313.7). Conclusion: A novel group of ferrocene compounds, possessing a methyl sulfonyl COX-2 pharmacophore were synthesized to investigate the effect of different substituents on selectivity and potency of COX-2 inhibitory activity and their cytotoxicity effects. This study indicates that 1-ferrocenyl-3-amino carbonyl compounds having ferrocene motif and methyl sulfonyl COX-2 pharmacophore is a suitable scaffold to design COX-2 inhibitors and anti-cancer agents.

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