scholarly journals Studies on Optimization of Growth Parameters for L-Asparaginase Production byStreptomyces ginsengisoli

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Neelima Deshpande ◽  
Prachi Choubey ◽  
Manasi Agashe
Keyword(s):  
Anticancer Activity ◽  
Submerged Fermentation ◽  
Shake Flask ◽  
Growth Parameters ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Multiple Functions ◽  
Carbon And Nitrogen ◽  
Maximal Production

A species ofStreptomyces,Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.

scholarly journals Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 461 ◽  
Author(s):  
Soo Kweon Lee ◽  
Ju Hun Lee ◽  
Hyeong Ryeol Kim ◽  
Youngsang Chun ◽  
Ja Hyun Lee ◽  
...  
Keyword(s):  
Functional Foods ◽  
Casein Hydrolysate ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Cordyceps Militaris ◽  
Wild Type ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Bioactive Product ◽  
Submerged Conditions

Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, low productivity of cordycepin is a barrier to commercialization. In this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. The highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. In addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: pH 6, temperature of 25 °C, shaking speed of 150 rpm, and culture time of 6 days. Effects of medium component on cordycepin production were also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) were most effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement) with maximum cordycepin production of about 445 mg/L. In particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.

scholarly journals Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

2021 ◽  
Vol 18 (1) ◽  
pp. 185-195
Author(s):  
Gousiya Begum ◽  
Srinivas Munjam
Keyword(s):  
Wheat Bran ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Maximum Production ◽  
Fungal Strains ◽  
Enhanced Production

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.

scholarly journals Isolation and Optimization of Monascus ruber OMNRC45 for Red Pigment Production and Evaluation of the Pigment as a Food Colorant

2020 ◽  
Vol 10 (24) ◽  
pp. 8867
Author(s):  
Osama M. Darwesh ◽  
Ibrahim A. Matter ◽  
Hesham S. Almoallim ◽  
Sulaiman A. Alharbi ◽  
You-Kwan Oh
Keyword(s):  
Submerged Fermentation ◽  
Critical Factor ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Pigment Production ◽  
Red Pigment ◽  
Monascus Ruber ◽  
Carbon And Nitrogen ◽  
General Acceptance ◽  
Food Colorant

The color of food is a critical factor influencing its general acceptance. Owing to the effects of chemical colorants on health, current research is directly aimed at producing natural and healthy food colorants from microbial sources. A pigment-producing fungal isolate, obtained from soil samples and selected based on its rapidity and efficiency in producing red pigments, was identified as Monascus ruber OMNRC45. The culture conditions were optimized to enhance pigment production under submerged fermentation. The optimal temperature and pH for the highest red pigment yield were 30 °C and 6.5, respectively. The optimum carbon and nitrogen sources were rice and peptone, respectively. The usefulness of the pigment produced as a food colorant was evaluated by testing for contamination by the harmful mycotoxin citrinin and assessing its biosafety in mice. In addition, sensory evaluation tests were performed to evaluate the overall acceptance of the pigment as a food colorant. The results showed that M. ruber OMNRC45 was able to rapidly and effectively produce dense natural red pigment under the conditions of submerged fermentation without citrinin production. The findings of the sensory and biosafety assessments indicated the biosafety and applicability of the red Monascus pigment as a food colorant.

scholarly journals Lab-Scale Optimization of Polyhydroxyalkanoate Production by Bacterial Strain cmg1415 on Local Cheap Substrates Using One Variable at a Time Approach

2021 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
Muhammadi Muhammadi ◽  
Shabina Shafiq
Keyword(s):  
Sugar Beet ◽  
Minimal Medium ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Sole Source ◽  
Labor Cost ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Scale Optimization

Production of polyhydroxyalkanoate (PHA) under optimum culture conditions using local cheap feedstocks is indispensable to overcome the current cost of PHA-based plastics. For this purpose, optimum culture conditions and cheap feedstocks were investigated to produce maximum yield of PHA in CMG1415. Maximum yield was obtained with sucrose or sugar beet as sole source of precursors for PHA in 8 days of incubation at 35 °C in a minimal medium adjusted at pH 7. Further, for maximum yield no mechanical shaking was needed. Local cheap feedstock such as sugar beet and molasses were found to play as significant carbon and nitrogen sources for maximum PHA yield.  Bacterial plastic produced under these low-labor-cost culture conditions may to reduce the present cost of degradable bioplastic and be much effective alternate of nondegradable varieties of synthetic plastic.

scholarly journals Investigating the expression of F10 and G11 xylanases in Aspergillus niger A09 with qPCR

2016 ◽  
Vol 62 (9) ◽  
pp. 744-752
Author(s):  
Shixiu Cui ◽  
Tianwen Wang ◽  
Hong Hu ◽  
Liangwei Liu ◽  
Andong Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Niger ◽  
Culture Media ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Transcript Abundance ◽  
Culture Conditions ◽  
Clear Understanding ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

There exist significant differences between the 2 main types of xylanases, family F10 and G11. A clear understanding of the expression pattern of microbial F10 and G11 under different culture conditions would facilitate better production and industrial application of xylanase. In this study, the fungal xylanase producer Aspergillus niger A09 was systematically investigated in terms of induced expression of xylanase F10 and G11. Results showed that carbon and nitrogen sources could influence xylanase F10 and G11 transcript abundance, with G11 more susceptible to changes in culture media composition. The most favorable carbon and nitrogen sources for high G11 and low F10 production by A. niger A09 were xylan (2%) and (NH4)2C2O4 (0.3%), respectively. Following cultivation at 33 °C for 60 h, the highest xylanase activity (1132 IU per gram of wet mycelia) was observed. On the basis of differential gene expression of F10 and G11, as well as their different properties, we deduced that the F10 protein initially targeted xylan and hydrolyzed it into fragments including xylose, after which xylose acted as the inducer of F10 and G11 gene expression. These speculations also accounted for our failure to identify conditions favoring the high production of F10 but a low production of G11.

scholarly journals Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Roheena Abdullah ◽  
Sobia Nazir Chudhary ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Wide Spectrum ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Fungal Strain ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Different Sources ◽  
Fermentation Media

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

scholarly journals Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ana Maria Mazotto ◽  
Rosalie Reed Rodrigues Coelho ◽  
Sabrina Martins Lage Cedrola ◽  
Marcos Fábio de Lima ◽  
Sonia Couri ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Soluble Protein ◽  
Submerged Fermentation ◽  
Nitrogen Sources ◽  
Poultry Industry ◽  
Feather Meal ◽  
Carbon And Nitrogen Sources ◽  
Gelatinase Activity ◽  
Carbon And Nitrogen ◽  
Bacillus Spp

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

Production of a Bioflocculant by Penicillium Sp. HHE-P7 Using Sugarcane Molasses

2012 ◽  
Vol 518-523 ◽  
pp. 453-459
Author(s):  
Li Fan Liu
Keyword(s):  
Rotation Speed ◽  
Fermentation Broth ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Medium Components ◽  
Flocculating Activity ◽  
Initial Ph

Bioflocculant MBF7 was produced by a novel bioflocculant-producing microorganism HHE-P7. In order to reduce the bioflocculant producing cost, culture experiments were conducted. The effects of medium components including carbon and nitrogen sources as well as culture conditions such as pH of molasses diluents, cultivating temperature, inoculum size were investigated. The results showed when the molasses waste was diluted at COD concentration of 2000 mg/L, the optimal culture conditions for MBF7 production by HHE-P7 were inoculum size 1% (v/v), initial pH 5, cultivating temperature 25°C at the rotation speed 150 r/min. Under such conditions, MBF7 had a flocculating activity of 83% for 5 g/L kaolin clay suspension. About 3.19 g crude bioflocculant could be recovered from 1.0 L of molasses fermentation broth.

scholarly journals Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Arotupin Daniel Juwon ◽  
Ogunmolu Funso Emmanuel
Keyword(s):  
Submerged Fermentation ◽  
Culture Media ◽  
Trichoderma Viride ◽  
Nitrogen Sources ◽  
Experimental Investigations ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Highly Active ◽  
Commercial Carbon ◽  
Polygalacturonase Production

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

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