scholarly journals Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Arotupin Daniel Juwon ◽  
Ogunmolu Funso Emmanuel
Keyword(s):  
Submerged Fermentation ◽  
Culture Media ◽  
Trichoderma Viride ◽  
Nitrogen Sources ◽  
Experimental Investigations ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Highly Active ◽  
Commercial Carbon ◽  
Polygalacturonase Production

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

Effect of nutritional factors on DDT-degradation by Mucor alternans

1971 ◽  
Vol 17 (10) ◽  
pp. 1291-1298 ◽  
Author(s):  
J. P. E. Anderson ◽  
E. P. Lichtenstein
Keyword(s):  
Ammonium Nitrate ◽  
Culture Media ◽  
Fungal Spores ◽  
Nitrogen Sources ◽  
Water Soluble ◽  
Carbon And Nitrogen Sources ◽  
Nutritional Factors ◽  
Carbon And Nitrogen ◽  
Insecticide Concentration ◽  
Mycelial Mass

The effects of nutritional factors on the capacity of Mucor alternans to degrade 14C-DDT in shake cultures were investigated. Fungal spores did not grow in media that contained DDT as the sole carbon source, but they remained viable over a 2-month period and developed into a mycelium, once glucose had been added. The degradation of DDT by the fungus was not related to the mycelial mass. The quantity of water-soluble metabolites produced from DDT was, to some extent, dependent on the insecticide concentration, but largely on the carbon and nitrogen sources in the culture media. Largest quantities of the metabolites were formed with glucose and ammonium nitrate. The concentration of glucose affected this metabolism quantitatively. With the exception of ribose, growth on other sugars resulted in a decrease in the production of DDT-metabolites, which was most noticeable when maltose was used. No qualitative differences in the metabolites were observed. When ammonium nitrate was replaced with other nitrogen sources, the production of water-soluble metabolites was substantially reduced (35 to 75%) and qualitative differences in the appearance of metabolites were also observed.

scholarly journals Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

2021 ◽  
Vol 18 (1) ◽  
pp. 185-195
Author(s):  
Gousiya Begum ◽  
Srinivas Munjam
Keyword(s):  
Wheat Bran ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Maximum Production ◽  
Fungal Strains ◽  
Enhanced Production

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.

Improvement of biomass and cyclic lipopeptides production in Bacillus amyloliquefaciens MEP218 by modifying carbon and nitrogen sources and ratios of the culture media

2017 ◽  
Vol 115 ◽  
pp. 119-128 ◽  
Author(s):  
Daniela B. Medeot ◽  
Magdalena Bertorello-Cuenca ◽  
Juan Pablo Liaudat ◽  
Florencia Alvarez ◽  
María Laura Flores-Cáceres ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Culture Media ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Cyclic Lipopeptides

Effect of carbon, nitrogen and physico-chemical factors on patulin production in Penicillium expansum

2018 ◽  
Vol 7 (2) ◽  
pp. 57-63
Author(s):  
Aouatef Mansouri Mansouri ◽  
Miloud Elkarbane ◽  
Mohamed Ben Aziz ◽  
Hasna Nait M’Barek ◽  
Majida Hafidi ◽  
...  
Keyword(s):  
Culture Media ◽  
Effective Means ◽  
Great Influence ◽  
Nitrogen Sources ◽  
Medium Effect ◽  
Penicillium Expansum ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio

Environmental and nutritional factors play important roles in regulating production of mycotoxins. Few studies have been reported on the biosynthesis of patulin mycotoxin and the mechanisms that involve its biosynthesis in Penicillium expansum. Here, we investigated the effects of two culture media, pH, temperature, carbon and nitrogen sources and effect of carbon/nitrogen ratio on mycotoxin biosynthesis by P. expansum isolated from Moroccan cereals. It was found that pH and temperature had great influence on patulin production. Results also showed that carbon and nitrogen sources influ-enced patulin biosynthesis significantly in this strain. L-glutamate was optimized as important nitrogen source in synthetic culture medium. Effect of carbon/nitrogen ratio was evaluated which indicated the dependence of patulin production on this ratio. These results will provide useful information to better understand the regulatory mechanisms of patulin biosynthesis, and be helpful in developing effective means for controlling a mycotoxin contam-ination of foods and feeds.

scholarly journals Investigating the expression of F10 and G11 xylanases in Aspergillus niger A09 with qPCR

2016 ◽  
Vol 62 (9) ◽  
pp. 744-752
Author(s):  
Shixiu Cui ◽  
Tianwen Wang ◽  
Hong Hu ◽  
Liangwei Liu ◽  
Andong Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Niger ◽  
Culture Media ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Transcript Abundance ◽  
Culture Conditions ◽  
Clear Understanding ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

There exist significant differences between the 2 main types of xylanases, family F10 and G11. A clear understanding of the expression pattern of microbial F10 and G11 under different culture conditions would facilitate better production and industrial application of xylanase. In this study, the fungal xylanase producer Aspergillus niger A09 was systematically investigated in terms of induced expression of xylanase F10 and G11. Results showed that carbon and nitrogen sources could influence xylanase F10 and G11 transcript abundance, with G11 more susceptible to changes in culture media composition. The most favorable carbon and nitrogen sources for high G11 and low F10 production by A. niger A09 were xylan (2%) and (NH4)2C2O4 (0.3%), respectively. Following cultivation at 33 °C for 60 h, the highest xylanase activity (1132 IU per gram of wet mycelia) was observed. On the basis of differential gene expression of F10 and G11, as well as their different properties, we deduced that the F10 protein initially targeted xylan and hydrolyzed it into fragments including xylose, after which xylose acted as the inducer of F10 and G11 gene expression. These speculations also accounted for our failure to identify conditions favoring the high production of F10 but a low production of G11.

scholarly journals Studies on Optimization of Growth Parameters for L-Asparaginase Production byStreptomyces ginsengisoli

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Neelima Deshpande ◽  
Prachi Choubey ◽  
Manasi Agashe
Keyword(s):  
Anticancer Activity ◽  
Submerged Fermentation ◽  
Shake Flask ◽  
Growth Parameters ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Multiple Functions ◽  
Carbon And Nitrogen ◽  
Maximal Production

A species ofStreptomyces,Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.

scholarly journals Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Roheena Abdullah ◽  
Sobia Nazir Chudhary ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Wide Spectrum ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Fungal Strain ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Different Sources ◽  
Fermentation Media

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

scholarly journals Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ana Maria Mazotto ◽  
Rosalie Reed Rodrigues Coelho ◽  
Sabrina Martins Lage Cedrola ◽  
Marcos Fábio de Lima ◽  
Sonia Couri ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Soluble Protein ◽  
Submerged Fermentation ◽  
Nitrogen Sources ◽  
Poultry Industry ◽  
Feather Meal ◽  
Carbon And Nitrogen Sources ◽  
Gelatinase Activity ◽  
Carbon And Nitrogen ◽  
Bacillus Spp

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

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