scholarly journals Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

2021 ◽  
Vol 18 (1) ◽  
pp. 185-195
Author(s):  
Gousiya Begum ◽  
Srinivas Munjam
Keyword(s):  
Wheat Bran ◽  
Submerged Fermentation ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Maximum Production ◽  
Fungal Strains ◽  
Enhanced Production

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.

scholarly journals Caseinase Production and Media Optimization from Bacillus subtilis

2020 ◽  
Vol 71 (11) ◽  
pp. 1-9
Author(s):  
Noor Nihad Abdul Hussein ◽  
Aseel I. Ibrahim ◽  
Firas Hashim Kamar ◽  
Arelia Cristina Nechifor
Keyword(s):  
Bacillus Subtilis ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
The Body ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Sequencing Result

Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat. Casein can be an instant energy source to the body and involves in muscle building. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. This is followed by the production of enzyme and its purification. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.

scholarly journals Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

2019 ◽  
Vol 35 (5) ◽  
Author(s):  
Roheena Abdullah ◽  
Sobia Nazir Chudhary ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Wide Spectrum ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Fungal Strain ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Different Sources ◽  
Fermentation Media

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

scholarly journals Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
S. P. Gautam ◽  
P. S. Bundela ◽  
A. K. Pandey ◽  
Jamaluddin Khan ◽  
M. K. Awasthi ◽  
...  
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Enzyme Production ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Production Medium ◽  
Optimum Temperature ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Alternative Carbon Source

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

Production of chemotherapeutic enzyme L-asparaginase from fungal source

2021 ◽  
Vol 22 ◽  
Author(s):  
Girish Bhikan Pendharkar ◽  
Harshal Gotu Sonawane ◽  
Kalpesh Ashok Khare ◽  
Dhananjay Yadav
Keyword(s):  
Aspergillus Flavus ◽  
Enzyme Production ◽  
Lymphoblastic Leukemia ◽  
Antineoplastic Agent ◽  
Nitrogen Sources ◽  
Shake Flask Culture ◽  
Substrate Affinity ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Fungal Enzyme

Background: L-Asparaginase is an antineoplastic agent used in the treatment of acute myeloid and acute lymphoblastic leukemia. The present study deals with the production of this chemotherapeutic enzyme drug from Aspergillus flavus NCIM 526. The production of enzymes was carried out using oil-extracted cakes in shake flask culture. Process parameters like carbon and nitrogen sources were taken into account. Methods: A total of six isolates were used to screen out efficient microorganisms for enzyme production. Aspergillus flavus NCIM 526 exhibited 138 IU/ml of enzyme activity in oil extracted mix cake after 96 hours of the incubation period. Molasses and l-asparagine were proved the best carbon and nitrogen sources for enzyme production. The enzyme was purified by column chromatography and the finest enzyme exhibited specific activity of 28 IU/mg. Results and Discussion: The fungal enzyme exhibited low Km values as compared with standard E. coli L-asparaginase, proving more substrate affinity of fungal enzyme than bacterial enzymes. Conclusion: The study explored the Aspergillus flavus NCIM 526 as a potential fungal source for high yield production of antileukemic enzyme drugs.

scholarly journals Comparative Studies on Effect of Carbon and Nitrogen Sources on L -Asparaginase Production

2017 ◽  
Vol 4 (4) ◽  
pp. 452-457 ◽  
Author(s):  
M. Mohamed Mahroop Raja ◽  
A. Raja ◽  
S. Mohamed Salique ◽  
P. Gajalakshmi
Keyword(s):  
Enzyme Assay ◽  
Nitrogen Sources ◽  
Gulf Of Mannar ◽  
Marine Actinomycetes ◽  
Phenotypic Characteristics ◽  
Purification And Characterization ◽  
Carbon And Nitrogen Sources ◽  
Streptomyces Sp ◽  
Carbon And Nitrogen ◽  
Maximum Production

Marine actinomycetes sediment samples were collected from Gulf of Mannar costal region, Kayalpatinam, located at Tuticorin district, Tamilnadu, India. Marine actinomycetes were isolated and evaluated for activity of L-asparaginase production. A total of 10 marine actinomycetes strains were isolated. Among 10 isolates, six were belongs to Streptomyces sp, three were belongs to Micromonospora sp and one was to Micropolyspora sp. Based on phenotypic characteristics, actinomycetes strains were screened for L-asparaginase production. Streptomyces sp KPMS5 and Micromonospora sp KPMS10 were showed large pink coloration on L-asparaginase production medium. The strains were further studied for maximum production and characterizations of culture condition of L-asparaginase enzyme were evaluated. Effect of substrate on L-asparaginase production was evaluated by enzyme assy. Maximum enzyme assay (1.4 mM) was observed on glucose followed by starch (1.12Mm) by Micromonospora sp KPMS10. In Streptomyces sp KPMS5 showed maximum of 1.25mM of enzyme assay on glucose substrate followed by lactose 1.17mM. Yeast extract was effectively used as substrate for maximum production of L-asparaginase by submerged fermentation. Further studies on purification and characterization are required to support the application of enzyme. The finding concludes isolates belongs to non-Streptomyces sp like Micromonospora sp is a potential novel source for L-asparaginase production.Int J Appl Sci Biotechnol, Vol 4(4): 452-457

scholarly journals Studies on Optimization of Growth Parameters for L-Asparaginase Production byStreptomyces ginsengisoli

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Neelima Deshpande ◽  
Prachi Choubey ◽  
Manasi Agashe
Keyword(s):  
Anticancer Activity ◽  
Submerged Fermentation ◽  
Shake Flask ◽  
Growth Parameters ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Multiple Functions ◽  
Carbon And Nitrogen ◽  
Maximal Production

A species ofStreptomyces,Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by “shake flask” method. The quantity of L-asparaginase produced was estimated as 3.23 μmol/mL/min. The effect of various culture conditions on L-asparaginase production was studied by adopting a method of variation in one factor at a time. Of the various conditions tested, glucose (followed by starch) and peptone served as good carbon and nitrogen sources, respectively, for maximal production of enzyme at pH 8. The temperature of 30°C and an incubation period of 5 days with 0.05 g% asparagine concentration were found to be optimum for L-asparaginase production.

scholarly journals Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ana Maria Mazotto ◽  
Rosalie Reed Rodrigues Coelho ◽  
Sabrina Martins Lage Cedrola ◽  
Marcos Fábio de Lima ◽  
Sonia Couri ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Soluble Protein ◽  
Submerged Fermentation ◽  
Nitrogen Sources ◽  
Poultry Industry ◽  
Feather Meal ◽  
Carbon And Nitrogen Sources ◽  
Gelatinase Activity ◽  
Carbon And Nitrogen ◽  
Bacillus Spp

Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

scholarly journals Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

2021 ◽  
Author(s):  
Mahdi Hamed ◽  
Ahmed A Osman ◽  
Mustafa Ateş
Keyword(s):  
Enzyme Activity ◽  
Enzyme Production ◽  
Enzyme Level ◽  
Nitrogen Sources ◽  
Quantitative Detection ◽  
Phenol Red ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Fungal Isolates ◽  
The Impact

AbstractObjectiveTo semi-quantitively screen filamentous fungi isolated from different habitats for L-asparaginase production by three indicators; phenol red, cresol red and bromothymol blue and to examine the impact of different carbon and nitrogen sources on the enzyme production using different fungal isolates.Materials and methodsFifty-five fungal isolates were tested for L-asparaginase production by plate assay using Modified Czapek-Dox (MCD) medium. The enzyme activity was estimated using the Nessler method which measures the concentration of ammonia formed owing to the enzyme action on the substrate. The impact of nitrogen and carbon sources on the enzyme production was done by using the best three L-asparaginase producers from the semi-quantitative screening.Results and conclusionsA total of 53/55 (96.36%) fungal isolates were L-asparaginase producing strains, of them, Cladosporium tenuissimum, Penicillium camembertii and Aspergillus carneus showed high enzyme production. Production of L-asparaginase was higher with the glucose and urea as carbon and nitrogen sources, respectively. The highest enzyme level (5,558 U/ml) was produced by C. tenuissimum in a glucose-containing medium. This study shows that P. camemberti, A. carneus, and C. tenuissimum are good L-asparaginase producers and thus could be used for L-asparaginase production

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