scholarly journals Effect of Strontium Chloride on Experimental Bladder Inflammation in Rat

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Esat Korgali ◽  
Gokce Dundar ◽  
Kubra Acikalin Coskun ◽  
Melih Akyol ◽  
Yusuf Tutar ◽  
...  
Keyword(s):  
Interstitial Cystitis ◽  
Control Group ◽  
Strontium Chloride ◽  
Sensory Irritation ◽  
E Coli ◽  
The Third ◽  
Chemically Induced ◽  
Definitive Therapy ◽  
Potential Use

Introduction. Strontium salts are anti-irritants for chemically induced sensory irritation. Interstitial cystitis is a painful disease without definitive therapy. The aim of the study was to determine the effect of strontium in bladder with experimental interstitial cystitis model. Material and Methods. Rats’ bladders in control group were instilled with NaCl. Second group was instilled with E. coli LPS. Third group was instilled with strontium. Fourth group was initially instilled with strontium and then LPS. Fifth group was instilled with LPS initially and then strontium. Urine of rats was collected at the beginning and end of the study. Results. Histamine and TNF-α changes were statistically significant in the second group but were not significant in the third group. When we compared the histamine levels of second via fourth and fifth groups the changes were statistically not significant. When we compared the TNF-α levels of second via fourth and fifth groups the changes were statistically significant. Conclusions. In our model, strontium did not make any significant changes in histopathology or histamine levels; however, it significantly reduced the levels of TNF-α. Given the role of TNF-α in the physiopathology of interstitial cystitis, these results suggested that further studies are required to evaluate the potential use of strontium in the management of interstitial cystitis.

scholarly journals EnteropathogenicEscherichia coliAssociated with Diarrhea in Children in Cairo, Egypt

2011 ◽  
Vol 11 ◽  
pp. 2613-2619 ◽  
Author(s):  
Iman K. Behiry ◽  
Emad A. Abada ◽  
Entsar A. Ahmed ◽  
Rania S. Labeeb
Keyword(s):  
Chronic Diarrhea ◽  
Control Group ◽  
Healthy Children ◽  
Disc Diffusion ◽  
Pediatric Hospital ◽  
E Coli ◽  
The Third ◽  
Different Seasons ◽  
One Year ◽  
Third Generation Cephalosporins

In this study we isolate and identify the EnteropathogenicEscherichia coli(EPEC) causing diarrhea in children less than five years in Cairo, Egypt, during different seasons. Children younger than five years with diarrhea, attending the Pediatric Gastroenterology Intensive Care Unit of the Cairo University Pediatric Hospital in one year period were our group of study. Our control group was age and sex matched concurrent healthy children. The identifiedE. coliisolates were subjected to antimicrobial disc diffusion susceptibility test and further identified for EPEC serotype by slide agglutination test, using antiserumE. colisomatic trivalent I (O111, O55, O26) according to the instructions of the manufacturer. Out of 134 patients 5.2% of them revealed EPEC in the fecal sample, while the 20 children control group showed no EPEC isolates in their samples. Our EPEC frequency showed variations from the compared results of other studies. Higher rate of EPEC (18.7%) was found in patients between 2 to 3 years, while EPEC rate was (7.5%) in patients less than 6 months old, with . EPEC was identified from fecal specimens as a unique pathogen or associated with other pathogens in acute and chronic diarrhea in children. EPEC were detected in all seasons except in winter, and was predominant in summer season. Four (57%) EPEC isolates were resistant to ampicillin, ticarcillin, and cotrimoxazole, and (14.3%) to the third generation cephalosporins.

scholarly journals The role of magnesium in the treatment of alcohol withdrawal with delirium

2021 ◽  
Vol 17 (8) ◽  
pp. 47-50
Author(s):  
O.O. Havrylov ◽  
M.L. Gomon
Keyword(s):  
Mean Arterial Pressure ◽  
Arterial Pressure ◽  
Magnesium Sulfate ◽  
Research Group ◽  
Alcohol Withdrawal ◽  
Serum Cortisol ◽  
Control Group ◽  
The Third ◽  
Significant Difference

Background. The role of magnesium in the treatment of alcohol withdrawal with delirium is indefinite, although it is well known about its participation in the pathogenesis of this severe condition. The study was aimed to reveal the benefits and disadvantages of the sedation with added magnesium sulfate in comparison with traditional sedative therapy. Materials and methods. In our study, we tested 40 treated patients, which were randomized in two groups. We controlled the level of sedation by the Richmond agitation-sedation scale. The target level of sedation was between 0 and –2 points. We maintained this level in all the patients. In all groups, we evaluated the following values: duration of delirium, mean arterial pressure, pulse, the level of serum magnesium, cortisol and serotonin, laboratory indicators of kidneys, and liver condition. In the control group, we carried out the sedation with 10–20 mg of diazepam every 4–6 hours with infusion of barbiturates as needed. The treatment in the research group was identical with addition of magnesium sulfate 50 mg/kg every 8 hours. Results. The results of the study demonstrated that the duration of delirium is significantly lower in the research group in comparison with the control (p < 0.05). We found hypomagnesemia in almost half of the patients. The indicators of haemodynamics such as mean arterial pressure and pulse were significantly lower in both groups on the third day (p < 0.05). We found 4 cases (20 %) of hypotension in the research group and 2 cases (10 %) in the control group. The study of the dynamics of serum cortisol and serotonin showed the significant difference on the third day in both groups (p < 0.05). We found no difference in these laboratory parameters on the third day between groups (p ≥ 0.05). There was no significant difference between the laboratory indicators of kidneys and liver condition in both groups (p ≥ 0.05). Conclusions. The use of magnesium sulfate allowed decreasing the duration of delirium but had an excessive effect on haemodynamics. Such biomarkers as serum cortisol and serotonin didn’t verify the effect of magnesium sulfate.

scholarly journals Peran Musik Dalam Rangka Meningkatkan Keyboarding Skills

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Sri Raharso ◽  
Sri Surjani Tjahjawati ◽  
Maya Setiawardani
Keyword(s):  
Significant Role ◽  
Rock Music ◽  
Control Group ◽  
The Third

The purpose of this research was to determine the role of music and genre of music that produces the fastest typing speeds in words per minute. Respondent of this research divided into two groups. The first group is a control group that learned keyboarding skill without music, the rest group learned keyboarding skill with music. The music that used in that experiments coming from Mavis Beacon Teaches Typing 26. The number of the word per minute for each respondent was recorded, three times, at the beginning, in the middle, and the end of the course; labeled by Kstart, Kmiddle, and Kend. In spite of identifying role of genre music to typing performance, the second group typed predetermined line while listening to different predetermined music. The genre categories uses were jazz, blues, rock, reggae, degung (Sundanese music), and classic. The number of words per minute for each respondent was recorded. Every respondent has three trials were run. The recorded information was compared. The result of this experiment showed that music played a significant role to shape typing performance. Second, this experiment showed that rock music produced the fastest typing speeds. Blues produced the second fastest typing speed. Jazz produces the third fastest typing speed.

scholarly journals Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Subhankari Prasad Chakraborty ◽  
Santanu KarMahapatra ◽  
Sumanta Kumar Sahu ◽  
Panchanan Pramanik ◽  
Somenath Roy
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Treated Group ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Viable Bacteria ◽  
Antioxidant Effects ◽  
Potential Use

Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 106 CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P<0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P<0.05); which were increased or decreased significantly (P<0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

scholarly journals Study of some Impact Of Enterococcus faecium As Probiotic On Chick 1- Body Weight And Pathological Changes In Air Sacs And Intestine

2007 ◽  
Vol 31 (1) ◽  
pp. 70-77
Author(s):  
Emad J. Khamas
Keyword(s):  
Body Weight ◽  
High Temperature ◽  
Enterococcus Faecium ◽  
Control Group ◽  
Pathological Changes ◽  
Fourth Group ◽  
E Coli ◽  
The Third ◽  
Air Sacs ◽  
The Impact

To investigate the impact of Enteococcus faecium as probiotic in layer chicks,this bacteria was given in feed at a dose rate of 1.5 gm per kg feed, each kg offeed contained 5×1011 cfu along the period of the study (57 days). 150 one dayold males were divided into five groups, 30 males each. The 1st group was giventhe probiotic only in the feed, the 2nd group was given the probiotic and exposedto high temperature and bad ventilation as a stress, and the third group wasgiven the probiotic with chlortetracycline in feed while the fourth group wasgiven the probiotic and then infected with E. coli on 21 day of age and the fifthgroup was given feed with out probiotic as the control group. The resultsshowed that there was increase in body weight especially in the last weeks of theexperiment. Turbidity of the air sacs was mild in the four group compared withcontrol. Intestinal villi became elongated due to using of probiotic and there wasless sloughing compared with control, and those groups showed resistancetoward E. coli infection.

scholarly journals The Role and Impact of Salivary Zn Levels on Dental Caries

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Milaim Sejdini ◽  
Agim Begzati ◽  
Sami Salihu ◽  
Sokol Krasniqi ◽  
Nora Berisha ◽  
...  
Keyword(s):  
Dental Caries ◽  
Maximum Concentration ◽  
Test Tube ◽  
Permanent Dentition ◽  
Control Group ◽  
The Third ◽  
Maximum Value ◽  
Zn Concentration ◽  
Dmft Index

Introduction. Minimal attention has been given to the role of salivary microelements, the importance they have in reducing the intensity of caries, and the effect of caries prophylaxes.Aim. This research aimed to determine the concentration and quantity of Zn and its impact on the prevention and the reduction of the intensity of caries in schoolchildren aged 12-13 years with permanent dentition.Methods. For this research, we analyzed the stimulated and nonstimulated full saliva of 106 schoolchildren divided into three groups by mean decayed, missing, and filled teeth (DMFT) index. The control group consisted of 25 caries-free children, the second group had 47 children with mean DMFT index of 1 to 6, and the third group had 34 children with DMFT index of ≥ 6. Complete saliva was collected from all children in a sterile test tube.Results. The concentration of Zn in saliva before stimulation in caries-free children has variations of the order of 0.001+ to 0.01 mmol/l. The maximum concentration after stimulation is 6.72 mmol/l, while the maximum value is 64.38 mmol/l.Conclusion. The Zn concentration in the stimulated saliva showed a significant increase in the group of caries-free children and could be described as a positive value for the reduction of caries.

scholarly journals The possibility of using Trichinella spiralis as an experimental model in the field of high dilutions

2021 ◽  
Vol 14 (2) ◽  
pp. 60-61
Author(s):  
Olga Borisovna Zhdanova ◽  
Amina Aslanovna Haidarova ◽  
Ludmila Alexandrovna Napisanova ◽  
Dimitri Rossohin ◽  
Olga Lozhenicina
Keyword(s):  
Conventional Treatment ◽  
Trichinella Spiralis ◽  
Stomach Contents ◽  
Control Group ◽  
Parasitic Disease ◽  
Wide Spread ◽  
Fourth Group ◽  
The Third ◽  
High Dilutions

Background Trichinosis is a parasitic disease caused by ingestion larvae of Trichinella spiralis (TS). In Russia trichinosis of pigs is widely spreaded. Invasion occurs when human or animal eat contaminated meat. TS is adapted to many hosts, and due to its wide spread, there has always been a demand of safe alternatives for this infection. A lot of information has been gathered about sharp decreasing of quantity of trichinae in muscles at repeated invasion of predators. We took a chance of applying antigen to TS as a vaccine, however, rate of immunogenetic effect wasn't high. Therefore, in an experimental work, we decided to inject vaccine and immunomodulators together at the same time. Material and Methods We subjected rats infected with TS to conventional treatment (vaccine) along with Cina 6cH. There were five groups as follows: The first group was injected antigen alone and no immunomodulators; the second group was injected antigen and Ronkoleikin; the third group was injected antigen and Sodium nucleinate, the fourth group was injected antigen and Cina C6cH; and the fifth group was a control group, which was injected neither antigen nor immunomodulators. After 21 days of vaccination (or giving homeopathic medicine) rats were contaminated TS in a dose of 200germs/animal. Results The rats were euthanized after two months. They we then analysed for stomach contents and quantity of worms in muscles. The highest number of worms were found in fifth group (no antigen and no immunomodulators) (more than 2600 worms/animal. The quantity of worms in the second group (injection of antigen and Ronkoleikin) was in four times less than in the control group. The most efficient substance was Ronkoleikin (900+160 worms/animal); Sodium nucleinate (1100+100 worms/per animal); Cina C6cH (1400+50 worms/animal). Conclusion Cina C6cH along with and others immunomodulators was able to exhibit protective effect against experimental trichinosis. Further research with rigorous designs is needed to explore the role of Cina in Trichinella spiralis.

scholarly journals Redox status of pregnant women with thrombophilia

2021 ◽  
pp. 1-1
Author(s):  
Aleksandra Dimitrijevic ◽  
Jovana Bradic ◽  
Vladimir Zivkovic ◽  
Aleksandra Dimitrijevic ◽  
Mirjana Milojevic-Corbic ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Biochemical Analysis ◽  
Reduced Glutathione ◽  
First Trimester ◽  
Normal Pregnancy ◽  
Redox Status ◽  
Control Group ◽  
Third Trimester ◽  
The Third

Background/Aim. Considering the fact that role of oxidative stress in pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of our study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women who were divided into two groups: thrombophilia and normal pregnancy group. The thrombophilia group consisted of 60 pregnant women with thrombophilia, while the normal pregnancy group included 60 physiologically healthy pregnant women. Blood samples for biochemical analysis were collected at the end of first, second and third trimester of pregnancy. Concentrations of hydrogen peroxide (H2O2), nitrites (NO2-) and index of lipid peroxidation measured as TBARS were measured in plasma. Level of reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT) were measured in erythrocytes. Results. In women with thrombophilia, NO2- values were increased in the first and third trimester when compared with healthy pregnant women (p<0.05). The higher levels of TBARS and H2O2 were noticed in women with thrombophilia in the first trimester when compared to healthy pregnant women (p<0.05). The values of SOD and CAT were lower in women with thrombophilia in the third and GSH in the first trimester compared to control group (p<0.05). Conclusion. Our results suggest increased generation of pro-oxidants in thrombophillia at the beginning of gestation, which declines as gestation progresses and reaches the similar values as in normal pregnancy at the end of pregnancy. Generally viewed, thrombophilia was associated with impaired antioxidant capacity - SOD and CAT were lower in the third and GSH in the first trimester compared to healthy women.

scholarly journals The Novel Role of Healing from Bacterial Infections of Lower Limb Open Fractures by X-Ray Exposure

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ali A. Mahdi ◽  
Tuqa S. Al-Salmani ◽  
Mustafa M. Al-Qaisi
Keyword(s):  
Lower Limb ◽  
Open Fractures ◽  
X Rays ◽  
Bacterial Isolates ◽  
Isolation Rate ◽  
X Ray ◽  
E Coli ◽  
The Third ◽  
Grade Ii

Introduction. An open fracture refers to a break in the skin, which is exposed to microbial contamination and eventually leads to most complicated infections. X-rays can kill bacteria by causing irreversible DNA damage. Objective. To confirm the role of X-ray exposure in treating infected wound fractures at the lower limb and determine X-ray exposure times. Methods. Fifty-one wound swabs were collected from patients with infected open fractures at the lower limb with grade II, IIIA, B, and C according to the Gustilo and Anderson classification system and then cultured. The bacterial isolates were identified by biochemical tests and the VITEK-2 System and tested against several antibiotics. The X-ray exposure was done for open fractures by radiography (at kV133 and 5 milliambers). Results. The higher isolation rate was recorded for Staphylococcus aureus with 21 (41.2%) isolates, and most of them (20, 95.2%) were isolated from grade II fractures. The isolation rate of Gram-negative bacteria was 25.5% for Escherichia coli with 13 isolates, 19.6% for Pseudomonas aeruginosa with 10 isolates, and 13.7% for Klebsiella pneumoniae with 7 isolates, most of which were isolated from grade III fractures. The isolation rate of P. aeruginosa was 60% (6 isolates) from grade IIIA and 71.4% (5 isolates) from grade IIIB for K. pneumoniae, while for E. coli it was 69.2% (9 isolates) from grade IIIC. All the bacterial isolates recorded high levels of antibiotic resistance against most tested antibiotics. Wound cultures of grade II fractures appeared sterile after the first X-ray exposure, and these wounds were infected with S. aureus or P. aeruginosa. However, cultures of grade IIIA and IIIB fractures appeared sterile after the second X-ray exposure for all isolated bacteria, except for S. aureus (grade IIIA fractures) (after the third X-ray exposure). Grade IIIC fractures showed sterile culture after the third X-ray exposure for wounds infected with P. aeruginosa and E. coli. Conclusions. The study concluded that X-ray exposure showed high effectiveness in treating infected open fractures.

scholarly journals The Terminalia laxiflora modulates the neurotoxicity induced by fipronil in male albino rats

2019 ◽  
Vol 39 (3) ◽  
Author(s):  
A.A. Khalaf ◽  
Mona K. Galal ◽  
Marwa A. Ibrahim ◽  
A.A. Abd Allah ◽  
Mostafa M. Afify ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Control Group ◽  
Albino Rats ◽  
Mrna Levels ◽  
The Third ◽  
Neurotoxic Effects ◽  
Mda Content

AbstractThe extensive use of fipronil (FPN) may trigger hazards to more than insects. The present investigation was carried out to evaluate the abrogating role of Terminalia laxiflora (TL) methanol extract (TLE) against the neurotoxic effects provoked by FPN. Fourty male albino rats were assigned into four equal groups. The first group served as control, the second one was orally administered FPN (10.5 mg/kg BW), the third group was given combination of FPN and TLE) (100 mg/kg BW), and the fourth one was orally given TLE. Our findings highlighted the efficacy of TLE as a neuroprotectant through a significant reduction in malondialdehyde (MDA) content by 25.8%, elevations of the reduced glutathione (GSH) level, catalase (CAT,) and superoxide dismutase (SOD) activities by 30.9, 41.2, and 48.2% respectively. Consequently, the relative mRNA levels of both Bax and caspase-3 were down-regulated by 40.54% and caspase-3 by 30.35% compared with the control group. Moreover, restoration of the pathological tissue injuries were detected. In conclusion, TLE proved to be a potent neuroprotective agent against the FPN-induced toxicity.

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