scholarly journals Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Yun Chen ◽  
Ya-Hui Tsai ◽  
Yuan-An Liu ◽  
Shih-Hua Lee ◽  
Sheng-Hong Tseng ◽  
...  
Keyword(s):  
3D Imaging ◽  
Imaging System ◽  
3D Structure ◽  
Three Dimensional ◽  
Spatial Relationship ◽  
Intestinal Transplantation ◽  
Muscle Layer ◽  
3D Images ◽  
Nerve Network ◽  
Transplant Patients

Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry. The crypt organoids and intestinal tissues showed well-defined 3D structures. The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features. In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor’s intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient’s intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor. The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection. In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients.

Validation of a Vision-Based, Three-Dimensional Facial Imaging System

2003 ◽  
Vol 40 (5) ◽  
pp. 523-529 ◽  
Author(s):  
A. Ayoub ◽  
A. Garrahy ◽  
C. Hood ◽  
J. White ◽  
M. Bock ◽  
...  
Keyword(s):  
3D Imaging ◽  
Cleft Lip ◽  
Imaging System ◽  
Three Dimensional ◽  
Procrustes Analysis ◽  
Facial Morphology ◽  
3D Images ◽  
Registration Errors ◽  
Measuring Machine ◽  
Full Face

Objective The aim of this study was to assess the accuracy of a newly developed three-dimensional (3D) imaging system in recording facial morphology. Methods Twenty-one infants with cleft lip each had a full-face alginate impression taken at the time of primary lip repair, and a stone cast was constructed from each impression. Five anthropometric points were marked on each cast. Each cast was digitized, and the 3D co-ordinates of the five points were obtained using a co-ordinate measuring machine (CMM, Ferranti) of documented accuracy (9.53 μm). Each cast was scanned in four positions using a computerized stereophotogrammetry (C3D) system. The five points were located on the 3D images, and their 3D co-ordinates were extracted by three operators. The co-ordinate systems produced by C3D were aligned, via translation and rotation, to match the CMM co-ordinate system using partial ordinary procrustes analysis. The displacements of the adjusted C3D co-ordinates from the reference co-ordinates were then measured. Three different types of errors were identified: operator, system, and registration errors. Results Operator error was within 0.2 mm of the true co-ordinates of the landmarks. C3D was accurate within 0.4 mm. The average displacement of points over the 21 casts at four positions for the three operators was 0.79 mm (median 0.68). Conclusions The presented 3D imaging system is reliable in recording facial deformity and could be utilized in recording cleft deformities and measuring the changes following surgery

scholarly journals The New Era of Three-Dimensional Histoarchitecture of the Human Endometrium

2021 ◽  
Vol 11 (8) ◽  
pp. 713
Author(s):  
Manako Yamaguchi ◽  
Kosuke Yoshihara ◽  
Nozomi Yachida ◽  
Kazuaki Suda ◽  
Ryo Tamura ◽  
...  
Keyword(s):  
3D Imaging ◽  
Relevant Literature ◽  
3D Structure ◽  
Three Dimensional ◽  
Human Endometrium ◽  
Tissue Structure ◽  
New Era ◽  
Whole Mount ◽  
Uterine Glands

The histology of the endometrium has traditionally been established by observation of two-dimensional (2D) pathological sections. However, because human endometrial glands exhibit coiling and branching morphology, it is extremely difficult to obtain an entire image of the glands by 2D observation. In recent years, the development of three-dimensional (3D) reconstruction of serial pathological sections by computer and whole-mount imaging technology using tissue clearing methods with high-resolution fluorescence microscopy has enabled us to observe the 3D histoarchitecture of tissues. As a result, 3D imaging has revealed that human endometrial glands form a plexus network in the basalis, similar to the rhizome of grass, whereas mouse uterine glands are single branched tubular glands. This review summarizes the relevant literature on the 3D structure of mouse and human endometrium and discusses the significance of the rhizome structure in the human endometrium and the expected role of understanding the 3D tissue structure in future applications to systems biology.

scholarly journals Three-dimensional imaging of hold baggage for airport security

2014 ◽  
Vol XL-5 ◽  
pp. 331-336
Author(s):  
S. Kolokytha ◽  
R. Speller ◽  
S. Robson
Keyword(s):  
3D Imaging ◽  
Imaging System ◽  
Tomographic Reconstruction ◽  
Three Dimensional ◽  
Handling Time ◽  
Cost Effective ◽  
Airport Security ◽  
X Ray ◽  
Common System ◽  
Close Range

This study describes a cost-effective check-in baggage screening system, based on "on-belt tomosynthesis" (ObT) and close-range photogrammetry, that is designed to address the limitations of the most common system used, conventional projection radiography. The latter's limitations can lead to loss of information and an increase in baggage handling time, as baggage is manually searched or screened with more advanced systems. This project proposes a system that overcomes such limitations creating a cost-effective automated pseudo-3D imaging system, by combining x-ray and optical imaging to form digital tomograms. Tomographic reconstruction requires a knowledge of the change in geometry between multiple x-ray views of a common object. This is uniquely achieved using a close range photogrammetric system based on a small network of web-cameras. This paper presents the recent developments of the ObT system and describes recent findings of the photogrammetric system implementation. Based on these positive results, future work on the advancement of the ObT system as a cost-effective pseudo-3D imaging of hold baggage for airport security is proposed.

scholarly journals Depth-varying Density and Organization of Chondrocytes in Immature and Mature Bovine Articular Cartilage Assessed by 3D Imaging and Analysis

2005 ◽  
Vol 53 (9) ◽  
pp. 1109-1119 ◽  
Author(s):  
Kyle D. Jadin ◽  
Benjamin L. Wong ◽  
Won C. Bae ◽  
Kelvin W. Li ◽  
Amanda K. Williamson ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
3D Imaging ◽  
Articular Surface ◽  
Three Dimensional ◽  
Neighboring Cell ◽  
3D Images ◽  
Bovine Articular Cartilage ◽  
Stages Of Growth ◽  
Cell Organization ◽  
Heterogeneous Tissue

Articular cartilage is a heterogeneous tissue, with cell density and organization varying with depth from the surface. The objectives of the present study were to establish a method for localizing individual cells in three-dimensional (3D) images of cartilage and quantifying depth-associated variation in cellularity and cell organization at different stages of growth. Accuracy of nucleus localization was high, with 99% sensitivity relative to manual localization. Cellularity (million cells per cm3) decreased from 290, 310, and 150 near the articular surface in fetal, calf, and adult samples, respectively, to 120, 110, and 50 at a depth of 1.0 mm. The distance/angle to the nearest neighboring cell was 7.9 μm/31°, 7.1 μm/31°, and 9.1 μm/31° for cells at the articular surface of fetal, calf, and adult samples, respectively, and increased/decreased to 11.6 μm/31°, 12.0 μm/30°, and 19.2 μm/25° at a depth of 0.7 mm. The methodologies described here may be useful for analyzing the 3D cellular organization of cartilage during growth, maturation, aging, degeneration, and regeneration.

scholarly journals A Review on Dual-Lens Fluorescence Microscopy for Three-Dimensional Imaging

2020 ◽  
Vol 8 ◽  
Author(s):  
Xiaoyan Li ◽  
Yubing Han ◽  
Wenjie Liu ◽  
Cuifang Kuang ◽  
Xu Liu ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
3D Imaging ◽  
Signal To Noise Ratio ◽  
Three Dimensional ◽  
Super Resolution ◽  
Light Sheet ◽  
Animal Cells ◽  
3D Images ◽  
Single Lens ◽  
Model Animal

Three-dimensional (3D) imaging using dual-lens fluorescence microscopies is popular in observing fluorescently labeled biological samples, such as mammalian/model animal cells, tissues, and embryos. Specifically, dual-lens super-resolution fluorescence microscopy methods using two opposing objective lenses allow significantly higher axial resolution and better signal to noise ratio than traditional single-lens counterparts, and thus distinguish more details in 3D images of fine intracellular structures. For 3D imaging of thick tissues and entire embryos, dual-lens light-sheet fluorescence microscopy methods using two objective lenses, either orthogonal or non-orthogonal, to achieve selective plane illumination, can meet the requirements, and thus can be used to observe embryo development and structures of interest in thick tissues. This review summarizes both dual-lens fluorescence microscopy methods, including their principles, configurations, and 3D imaging applications, providing a guideline for biological laboratories with different 3D imaging needs.

scholarly journals A Microwave Three-Dimensional Imaging Method Based on Optimal Wave Spectrum Reconstruction

Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7306
Author(s):  
Yan Zhang ◽  
Baoping Wang ◽  
Yang Fang ◽  
Zuxun Song
Keyword(s):  
3D Imaging ◽  
Imaging System ◽  
Wave Spectrum ◽  
Sampling Rate ◽  
Three Dimensional ◽  
Imaging Method ◽  
Data Sampling ◽  
Spectrum Reconstruction ◽  
Memory Space ◽  
Target Wave

Limited by the Shannon–Nyquist sampling law, the number of antenna elements and echo signal data of the traditional microwave three-dimensional (3D) imaging system are extremely high. Compressed sensing imaging methods based on sparse representation of target scene can reduce the data sampling rate, but the dictionary matrix of these methods takes a lot of memory, and the imaging has poor quality for continuously distributed targets. For the above problems, a microwave 3D imaging method based on optimal wave spectrum reconstruction and optimization with target reflectance gradient is proposed in this paper. Based on the analysis of the spatial distribution characteristics of the target echo in the frequency domain, this method constructs an orthogonal projection reconstruction model for the wavefront to realize the optimal reconstruction of the target wave spectrum. Then, the inverse Fourier transform of the optimal target wave spectrum is optimized according to the law of the target reflectance gradient distribution. The proposed method has the advantages of less memory space and less computation time. What is more, the method has a better imaging quality for the continuously distributed target. The computer simulation experiment and microwave anechoic chamber measurement experiment verify the effectiveness of the proposed method.

scholarly journals Adaptive 3D Imaging for Moving Targets Based on a SIMO InISAR Imaging System in 0.2 THz Band

2021 ◽  
Vol 13 (4) ◽  
pp. 782
Author(s):  
Hongwei Li ◽  
Chao Li ◽  
Shiyou Wu ◽  
Shen Zheng ◽  
Guangyou Fang
Keyword(s):  
Synthetic Aperture Radar ◽  
3D Imaging ◽  
Imaging System ◽  
Synthetic Aperture ◽  
Moving Targets ◽  
Thz Imaging ◽  
Inverse Synthetic Aperture Radar ◽  
3D Images ◽  
Imaging Results ◽  
Aperture Radar

Terahertz (THz) imaging technology has received increased attention in recent years and has been widely applied, whereas the three-dimensional (3D) imaging for moving targets remains to be solved. In this paper, an adaptive 3D imaging scheme is proposed based on a single input and multi-output (SIMO) interferometric inverse synthetic aperture radar (InISAR) imaging system to achieve 3D images of moving targets in THz band. With a specially designed SIMO antenna array, the angular information of the targets can be determined using the phase response difference in different receiving channels, which then enables accurate tracking by adaptively adjusting the antenna beam direction. On the basis of stable tracking, the high-resolution imaging can be achieved. A combined motion compensation method is proposed to produce well-focused and coherent inverse synthetic aperture radar (ISAR) images from different channels, based on which the interferometric imaging is performed, thus forming the 3D imaging results. Lastly, proof-of-principle experiments were performed with a 0.2 THz SIMO imaging system, verifying the effectiveness of the proposed scheme. Non-cooperative moving targets were accurately tracked and the 3D images obtained clearly identify the targets. Moreover, the dynamic imaging results of the moving targets were achieved. The promising results demonstrate the superiority of the proposed scheme over the existing THz imaging systems in realizing 3D imaging for moving targets. The proposed scheme shows great potential in detecting and monitoring moving targets with non-cooperative movement, including unmanned military vehicles and space debris.

scholarly journals Wavelength-Selective Phase-Shifting Digital Holography: Color Three-Dimensional Imaging Ability in Relation to Bit Depth of Wavelength-Multiplexed Holograms

2018 ◽  
Vol 8 (12) ◽  
pp. 2410 ◽  
Author(s):  
Tatsuki Tahara ◽  
Reo Otani ◽  
Yasuhiro Takaki
Keyword(s):  
Digital Holography ◽  
3D Imaging ◽  
Dynamic Range ◽  
Three Dimensional ◽  
Image Sensor ◽  
Phase Shifting ◽  
3D Images ◽  
Wavelength Resolution ◽  
The Relationship

The quality of reconstructed images in relation to the bit depth of holograms formed by wavelength-selective phase-shifting digital holography was investigated. Wavelength-selective phase-shifting digital holography is a technique to obtain multiwavelength three-dimensional (3D) images with a full space-bandwidth product of an image sensor from wavelength-multiplexed phase-shifted holograms and has been proposed since 2013. The bit resolution required to obtain a multiwavelength holographic image was quantitatively and experimentally evaluated, and the relationship between wavelength resolution and dynamic range of an image sensor was numerically simulated. The results indicate that two-bit resolution per wavelength is required to conduct color 3D imaging.

In SituX-Ray Tomography Measurements of Deformation in Cellular Solids

MRS Bulletin ◽  
2003 ◽  
Vol 28 (4) ◽  
pp. 284-289 ◽  
Author(s):  
E. Maire ◽  
A. Elmoutaouakkil ◽  
A. Fazekas ◽  
L. Salvo
Keyword(s):  
3D Imaging ◽  
Recent Literature ◽  
Three Dimensional ◽  
Cellular Solids ◽  
Aluminum Foams ◽  
3D Images ◽  
Closed Cell ◽  
Pertinent Information ◽  
Deformation Modes

AbstractThe use of microtomography to study the structure and especially the deformation modes of cellular solids is reviewed in this article. First, the technique is described in detail. Examples illustrating the power of the coupling ofin situdeformation with three-dimensional (3D) imaging, drawn from the recent literature and the authors' own work, are then given. The most detailed example is the study of the deformation modes of several samples made of different aluminum foams. Four kinds of closed-cell foams were investigated, corresponding to different routes available today for their manufacture. The initial macrostructure was quantified using the 3D images combined with 3D granulometry, allowing retrieval of pertinent information about the cell size and the wall and strut thicknesses. The global behavior exhibited by the foams during thein situcompression experiments was shown to vary from one brand of material to another. Some of these variations can be explained by differences in the known microstructure and the measured macrostructure of the samples.

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