scholarly journals Wavelength-Selective Phase-Shifting Digital Holography: Color Three-Dimensional Imaging Ability in Relation to Bit Depth of Wavelength-Multiplexed Holograms

2018 ◽  
Vol 8 (12) ◽  
pp. 2410 ◽  
Author(s):  
Tatsuki Tahara ◽  
Reo Otani ◽  
Yasuhiro Takaki

The quality of reconstructed images in relation to the bit depth of holograms formed by wavelength-selective phase-shifting digital holography was investigated. Wavelength-selective phase-shifting digital holography is a technique to obtain multiwavelength three-dimensional (3D) images with a full space-bandwidth product of an image sensor from wavelength-multiplexed phase-shifted holograms and has been proposed since 2013. The bit resolution required to obtain a multiwavelength holographic image was quantitatively and experimentally evaluated, and the relationship between wavelength resolution and dynamic range of an image sensor was numerically simulated. The results indicate that two-bit resolution per wavelength is required to conduct color 3D imaging.

2010 ◽  
Vol 6 (10) ◽  
pp. 472-478 ◽  
Author(s):  
Takashi Kakue ◽  
Kenichi Ito ◽  
Tatsuki Tahara ◽  
Yasuhiro Awatsuji ◽  
Kenzo Nishio ◽  
...  

Author(s):  
Roghayeh Yazdani ◽  
Hamidreza Fallah

In digital holography, errors of the reference field degrade the quality of the reconstructed object field. In this paper, we propose an effective method in phase-shifting digital holography in which the reference field does not need to be known and perfect. The unknown complex amplitudes of both reference and object fields are derived simultaneously. The method employs only five digital holograms and a single execution of a phase retrieval algorithm. So, the required measurements and algorithm executions in this method are fewer than those in other methods; it suggests a simpler and faster method. The effectiveness of the suggested method is indicated by simulation, under noise-free and noisy conditions. Moreover, the capability of the method to extract full information about the phase singularities in both fields is demonstrated.


2018 ◽  
Vol 212 ◽  
pp. 04005
Author(s):  
Andrey Bolshakov

Space is the main material with which the architect works. The space organized by the means of architecture is an art environment for the life of society. With many aspects of the organization of space, which are studied in the literature, the problem of their assembly and integration remains unsolved. The paper proposes a method of assembling spatial representations in architecture-the correlation of the spatial lattice and the factors of its form-formation, considered in the system, i.e. together. The approach is that in a broad overview of the world architecture, both in its theory and in practice, from historical to modern, examples of modification of spatial grids under the influence of one or a group of dominant factors are revealed. As a result, provisions on the relationship of the geometry of spatial grids with the following factors have been revealed: publicity and privacy; architectonics, the influence of the lattice on the differentiation of the streams of human movement; the architecture’s ability to carry a message; navigation properties, evaluation of connectivity and centrality of places in the spatial lattice; evaluation of the quality of the composition through the identification of ways of order and randomness in the elements of the spatial lattice. The results of the work can be used both for theoretical understanding of the architectural space in the study and design of architecture, and in architectural education.


2018 ◽  
Vol 57 (06) ◽  
pp. 1 ◽  
Author(s):  
Takahito Fukuda ◽  
Yasuhiro Awatsuji ◽  
Peng Xia ◽  
Takashi Kakue ◽  
Kenzo Nishio ◽  
...  

2005 ◽  
Vol 53 (9) ◽  
pp. 1109-1119 ◽  
Author(s):  
Kyle D. Jadin ◽  
Benjamin L. Wong ◽  
Won C. Bae ◽  
Kelvin W. Li ◽  
Amanda K. Williamson ◽  
...  

Articular cartilage is a heterogeneous tissue, with cell density and organization varying with depth from the surface. The objectives of the present study were to establish a method for localizing individual cells in three-dimensional (3D) images of cartilage and quantifying depth-associated variation in cellularity and cell organization at different stages of growth. Accuracy of nucleus localization was high, with 99% sensitivity relative to manual localization. Cellularity (million cells per cm3) decreased from 290, 310, and 150 near the articular surface in fetal, calf, and adult samples, respectively, to 120, 110, and 50 at a depth of 1.0 mm. The distance/angle to the nearest neighboring cell was 7.9 μm/31°, 7.1 μm/31°, and 9.1 μm/31° for cells at the articular surface of fetal, calf, and adult samples, respectively, and increased/decreased to 11.6 μm/31°, 12.0 μm/30°, and 19.2 μm/25° at a depth of 0.7 mm. The methodologies described here may be useful for analyzing the 3D cellular organization of cartilage during growth, maturation, aging, degeneration, and regeneration.


2020 ◽  
Vol 8 ◽  
Author(s):  
Xiaoyan Li ◽  
Yubing Han ◽  
Wenjie Liu ◽  
Cuifang Kuang ◽  
Xu Liu ◽  
...  

Three-dimensional (3D) imaging using dual-lens fluorescence microscopies is popular in observing fluorescently labeled biological samples, such as mammalian/model animal cells, tissues, and embryos. Specifically, dual-lens super-resolution fluorescence microscopy methods using two opposing objective lenses allow significantly higher axial resolution and better signal to noise ratio than traditional single-lens counterparts, and thus distinguish more details in 3D images of fine intracellular structures. For 3D imaging of thick tissues and entire embryos, dual-lens light-sheet fluorescence microscopy methods using two objective lenses, either orthogonal or non-orthogonal, to achieve selective plane illumination, can meet the requirements, and thus can be used to observe embryo development and structures of interest in thick tissues. This review summarizes both dual-lens fluorescence microscopy methods, including their principles, configurations, and 3D imaging applications, providing a guideline for biological laboratories with different 3D imaging needs.


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