Effect of nutritional factors on DDT-degradation by Mucor alternans

1971 ◽  
Vol 17 (10) ◽  
pp. 1291-1298 ◽  
Author(s):  
J. P. E. Anderson ◽  
E. P. Lichtenstein
Keyword(s):  
Ammonium Nitrate ◽  
Culture Media ◽  
Fungal Spores ◽  
Nitrogen Sources ◽  
Water Soluble ◽  
Carbon And Nitrogen Sources ◽  
Nutritional Factors ◽  
Carbon And Nitrogen ◽  
Insecticide Concentration ◽  
Mycelial Mass

The effects of nutritional factors on the capacity of Mucor alternans to degrade 14C-DDT in shake cultures were investigated. Fungal spores did not grow in media that contained DDT as the sole carbon source, but they remained viable over a 2-month period and developed into a mycelium, once glucose had been added. The degradation of DDT by the fungus was not related to the mycelial mass. The quantity of water-soluble metabolites produced from DDT was, to some extent, dependent on the insecticide concentration, but largely on the carbon and nitrogen sources in the culture media. Largest quantities of the metabolites were formed with glucose and ammonium nitrate. The concentration of glucose affected this metabolism quantitatively. With the exception of ribose, growth on other sugars resulted in a decrease in the production of DDT-metabolites, which was most noticeable when maltose was used. No qualitative differences in the metabolites were observed. When ammonium nitrate was replaced with other nitrogen sources, the production of water-soluble metabolites was substantially reduced (35 to 75%) and qualitative differences in the appearance of metabolites were also observed.

Improvement of biomass and cyclic lipopeptides production in Bacillus amyloliquefaciens MEP218 by modifying carbon and nitrogen sources and ratios of the culture media

2017 ◽  
Vol 115 ◽  
pp. 119-128 ◽  
Author(s):  
Daniela B. Medeot ◽  
Magdalena Bertorello-Cuenca ◽  
Juan Pablo Liaudat ◽  
Florencia Alvarez ◽  
María Laura Flores-Cáceres ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Culture Media ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Cyclic Lipopeptides

Effect of carbon, nitrogen and physico-chemical factors on patulin production in Penicillium expansum

2018 ◽  
Vol 7 (2) ◽  
pp. 57-63
Author(s):  
Aouatef Mansouri Mansouri ◽  
Miloud Elkarbane ◽  
Mohamed Ben Aziz ◽  
Hasna Nait M’Barek ◽  
Majida Hafidi ◽  
...  
Keyword(s):  
Culture Media ◽  
Effective Means ◽  
Great Influence ◽  
Nitrogen Sources ◽  
Medium Effect ◽  
Penicillium Expansum ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Carbon Nitrogen Ratio ◽  
Nitrogen Ratio

Environmental and nutritional factors play important roles in regulating production of mycotoxins. Few studies have been reported on the biosynthesis of patulin mycotoxin and the mechanisms that involve its biosynthesis in Penicillium expansum. Here, we investigated the effects of two culture media, pH, temperature, carbon and nitrogen sources and effect of carbon/nitrogen ratio on mycotoxin biosynthesis by P. expansum isolated from Moroccan cereals. It was found that pH and temperature had great influence on patulin production. Results also showed that carbon and nitrogen sources influ-enced patulin biosynthesis significantly in this strain. L-glutamate was optimized as important nitrogen source in synthetic culture medium. Effect of carbon/nitrogen ratio was evaluated which indicated the dependence of patulin production on this ratio. These results will provide useful information to better understand the regulatory mechanisms of patulin biosynthesis, and be helpful in developing effective means for controlling a mycotoxin contam-ination of foods and feeds.

scholarly journals Investigating the expression of F10 and G11 xylanases in Aspergillus niger A09 with qPCR

2016 ◽  
Vol 62 (9) ◽  
pp. 744-752
Author(s):  
Shixiu Cui ◽  
Tianwen Wang ◽  
Hong Hu ◽  
Liangwei Liu ◽  
Andong Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Aspergillus Niger ◽  
Culture Media ◽  
Xylanase Activity ◽  
Nitrogen Sources ◽  
Transcript Abundance ◽  
Culture Conditions ◽  
Clear Understanding ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

There exist significant differences between the 2 main types of xylanases, family F10 and G11. A clear understanding of the expression pattern of microbial F10 and G11 under different culture conditions would facilitate better production and industrial application of xylanase. In this study, the fungal xylanase producer Aspergillus niger A09 was systematically investigated in terms of induced expression of xylanase F10 and G11. Results showed that carbon and nitrogen sources could influence xylanase F10 and G11 transcript abundance, with G11 more susceptible to changes in culture media composition. The most favorable carbon and nitrogen sources for high G11 and low F10 production by A. niger A09 were xylan (2%) and (NH4)2C2O4 (0.3%), respectively. Following cultivation at 33 °C for 60 h, the highest xylanase activity (1132 IU per gram of wet mycelia) was observed. On the basis of differential gene expression of F10 and G11, as well as their different properties, we deduced that the F10 protein initially targeted xylan and hydrolyzed it into fragments including xylose, after which xylose acted as the inducer of F10 and G11 gene expression. These speculations also accounted for our failure to identify conditions favoring the high production of F10 but a low production of G11.

scholarly journals Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Arotupin Daniel Juwon ◽  
Ogunmolu Funso Emmanuel
Keyword(s):  
Submerged Fermentation ◽  
Culture Media ◽  
Trichoderma Viride ◽  
Nitrogen Sources ◽  
Experimental Investigations ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Highly Active ◽  
Commercial Carbon ◽  
Polygalacturonase Production

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

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