scholarly journals Antioxidant Enriched Fractions fromZingiber OfficinaleRoscoe

2011 ◽  
Vol 8 (2) ◽  
pp. 721-726 ◽  
Author(s):  
Ismail Rahath Kubra ◽  
Kulathooran Ramalakshmi ◽  
Lingamallu Jagan Mohan Rao
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Zingiber Officinale ◽  
Pharmaceutical Formulations ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Methanol Fraction ◽  
Freeze Dried ◽  
Ginger Rhizome

Ginger rhizome (Zingiber officinaleRoscoe) has many diverse properties and medicinal values such as antioxidant potential combined with the properties of a spice. Dried ginger (DG) were extracted with aqueous ethanol and freeze-dried. The extract was evaluated for antioxidant potential, using 1,1'-diphenyl-2-picryl-hydrazyl radical scavenging, antioxidant capacity and reducing power assays. DG extract was further fractionated into methanol (Mfr) and water-soluble (Wfr) fractions. The Mfr exhibited higher antioxidant capacity when compared to DG extract. Higher antioxidant potential of the methanol fraction may be due to the presence higher polyphenols and [6]-gingerol content. This suggests that alcoholic soluble fraction possess enormous scope to enhance the antioxidant potential when used as a supplement in various food as well as pharmaceutical formulations / products.

Influence of extraction technique on the mineral content and antioxidant capacity of edible oil extracted from ginger rhizome

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Solvent Extraction ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Extraction Technique ◽  
Dpph Radical ◽  
Ginger Rhizome ◽  
Dpph Radical Scavenging ◽  
Solvent Solvent

Influence of various extraction techniques (solvent-solvent, cold maceration and soxhlet extraction) on the mineral content and antioxidant capacity of ginger oil was investigated. The minerals determined were magnesium, manganese, zinc, iron, calcium, potassium, sodium and phosphorus. Flavonoid and phenolic contents were determined alongside the antioxidant capacity of the oils which was assayed using DPPH radical scavenging activity and total reducing power. Extracted oil showed strong characteristic pungent gingerly odor and was liquids at room temperature. Highest oil yield was obtained by soxhlet extraction technique (8.04± 0.04%); followed by cold maceration technique (5.30±0.03%) and the lowest by solvent-solvent extraction technique (4.83±0.08%). The results of the mineral analysis showed that oil obtained by soxhlet extraction technique had the highest concentration of all minerals determined, except manganese (3.80±0.01 mg/100g) which was highest in the oil obtained by cold maceration. The oil obtained by solvent-solvent extraction showed lowest concentration of all minerals determined, except calcium (1.08±0.10 mg/100g) and zinc (0.63±0.11 mg/100g) which were lowest in oil obtained by cold maceration. However, the concentration of phosphorus was highest in all the oils when compared with other minerals. Oil obtained by soxhlet extraction showed the highest concentration of flavonoids (118.00±1.00 mgQE/g) and phenolics (217.33±1.53 mgGAE/g) as well as the highest average DPPH radical scavenging capacity (55.56±0.04%) and average total reducing power (0.88±0.002) while the least concentration of flavonoids (44.45±2.97 mgQE/g), phenolics (112.43±1.42 mgGAE/g), average DPPH radical scavenging capability (19.73±0.01%) and average total reducing power (0.55±0.030) were obtained for the oil obtained by solvent-solvent extraction technique. The results showed that heat and reflux condition involved in the soxhlet extraction technique enhanced the extraction of minerals and phytochemicals from ginger rhizome and also enhanced the antioxidant capacity of ginger rhizome oil.

scholarly journals DRYING EFFECTS ON ULTRASONIC ASSISTED PHENOLIC YIELDS AND RETENTIVENESS OF ANTIRADICAL PROPERTIES OF COMMON CULINARY SPICES GINGER (ZINGIBER OFFICINALE) AND TURMERIC (CURCUMA LONGO): HPTLC AND GC - MS PROFILE FOR THEIR ACTIVE INGREDIENTS ASSESSMENT

2017 ◽  
Vol 5 (9(SE)) ◽  
pp. 7-23
Author(s):  
Nagarajan Leebanon Poonkuil ◽  
J. Dhaveethu Raja
Keyword(s):  
Superoxide Radical ◽  
Antiradical Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Zingiber Officinale ◽  
Total Phenolic ◽  
Scavenging Capacity ◽  
Ginger Rhizome ◽  
Fresh State ◽  
Ultrasonic Assisted

The effect of drying on antiradical activity of Zingiber officinale (ginger) and Curcuma longo (turmeric) were studied by total phenolic content (TPC), total reducing power (TRP), 2,2'-azino-bis(3-ethyl benz thiazoline-6-sulphonicacid) (ABTS), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical scavenging assays. Comparing fresh and dried rhizome, optimum antiradical activity was observed in dry ginger and in fresh turmeric. The drying phenomenon diminished the scavenging capacity especially in turmeric and also ginger rhizome was exhibited highest superoxide radical scavenging solely at fresh state. The extraction parameters were standardized for maximum recovery of phenolics. The Zingiberene of ginger and curcumin of turmeric rummaged the free radicals energetically.

Differential Antioxidant Composition and Potential of some commonly used Indian Spices

2017 ◽  
Vol 4 (03) ◽  
Author(s):  
SHARDA CHOUDHARY ◽  
ANDY PEREIRA ◽  
SUPRATIM BASU ◽  
A. K. VERMA
Keyword(s):  
Antioxidant Capacity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Food Preservatives ◽  
Nitric Oxide Radical ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Spices and herbs have been added to Indian foods not only as flavoring agents but also as natural supplements of medicines and food preservatives. The purpose of this study was to study the composition and antioxidant potential of some commonly used seed spices and derive a correlation between their phytochemical components and antioxidant capacity. Crushed seeds of cumin (Cuminumcyminum), coriander (Coriandrumsativum), fennel (Foeniculumvulgare), fenugreek (Trigonellafoenum-graecum), ajwain (Trachyspermumammi) and turmeric (Curcuma longa)were usedto prepare methanolic extract and iron(III) reduction, DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, hydrogen peroxide , superoxide and nitric oxide radical scavenging, reducing power were assayed as an index of the antioxidant capacity along with phenolics and flavonoids were also estimated. In addition to turmeric, ajwain, fennel and coriander showed higher phenolic, flavonoid content, and antioxidant potential in comparison to fenugreek and cumin. The extracts of these spices are promising sources of alternative medicine with high free radical scavenging ability OF synthetic antioxidants and can also be used for therapeutic purposes as well.

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

Design and Synthesis of Novel Anti-inflammatory/Anti-ulcer Hybrid Molecules with Antioxidant Activity

2020 ◽  
Vol 16 ◽  
Author(s):  
Bhim Bahadur Chaudhari ◽  
Alka Bali ◽  
Ajitesh Balaini
Keyword(s):  
Antioxidant Activity ◽  
Oral Absorption ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Inflammatory Activity ◽  
In Vivo Models ◽  
Standard Drug ◽  
Hybrid Molecules ◽  
Anti Inflammatory

Background: NSAIDs are the most widely prescribed medications worldwide for their anti-inflammatory, antipyretic, and analgesic effects However, their chronic use can lead to several adverse drug events including GI toxicity. The selective COX-2 inhibitors developed as gastro-sparing NSAIDs also suffer from serious adverse effects which limit their efficacy. Objective: Local generation of reactive oxygen species is implicated in NSAID-mediated gastric ulceration and their combination with H2 antagonists like famotidine reduces the risk of ulcers. The objective of this work was to design and synthesize novel methanesulphonamido isoxazole derivatives by hybridizing the structural features of NSAIDs with those of antiulcer drugs (ranitidine, famotidine, etc.) to utilize a dual combination of anti-inflammatory activity and reducing (antioxidant) potential. Method: The designing process utilized three dimensional similarity studies and utilized an isoxazole core having a potential for anti-inflammatory as well as radical scavenging antioxidant activity. The compounds were assayed for their antiinflammatory activity in established in vivo models. The in vitro antioxidant activity was assessed in potassium ferricyanide reducing power (PFRAP) assay employing ascorbic acid as the standard drug. Results: Compounds (5, 6, 9 and 10) showed anti-inflammatory activity comparable to the standard drugs and were also found to be non-ulcerogenic at the test doses. Compounds 6-10 exhibited good antioxidant effect in the concentration range of 1.0-50.0 µmol/ml. The test compounds were also found to comply with the Lipinski rule suggesting good oral absorption. Conclusion: A new series of isoxazole based compounds is being reported with good anti-inflammatory activity coupled with antioxidant potential as gastro-sparing anti-inflammatory agents.

scholarly journals Evaluation of Phytochemical Content and Antioxidant Potential of Ocimum gratissimum and Telfairia occidentalis Leaves

2019 ◽  
pp. 1-11
Author(s):  
A. I. Airaodion ◽  
A. H. Ibrahim ◽  
U. Ogbuagu ◽  
E. O. Ogbuagu ◽  
O. O. Awosanya ◽  
...  
Keyword(s):  
Pharmacological Study ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Running Water ◽  
Phytochemical Content ◽  
Ocimum Gratissimum ◽  
Telfairia Occidentalis ◽  
Significant Difference ◽  
Ibadan Nigeria

Aim: The aim of this study is to compare the phytochemical content and antioxidant potential of Ocimum gratissimum and Telfairia occidentalis leaves. Study Design: This study was made to fit a one-way Analysis of Variance. Place and Duration of Study: This research was carried out in the Department of Premedical Science, Educational Advancement Centre, Ibadan and Pharmaceutical Laboratory of the University of Ibadan, Nigeria between January and June, 2018. Methods: Both plants were purchased from Bodija market in Ibadan, Nigeria. The leaves were removed from the stem and washed with running water to remove contaminants. It was oven dried at 37˚C and milled into powder and extracted with ethanol. The qualitative and quantitative analyses of the phytochemical content as well as antioxidant potential were investigated. Results: The result showed that O. gratissimum is significantly higher in flavonoids content but lower in alkaloids when compared with those of T. occidentalis at P<0.05. No significant difference was observed in the concentrations of saponin, tannin, total phenolics and phytic acid in O. gratissimum when compared with those of T. occidentalis respectively at P<0.05. Antioxidant investigation showed that O. gratissimum is higher in ferric-ion reducing power but lower in ascorbic acid when compared with T. occidentalis respectively at P<0.05. The percentage inhibition of 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging potential was observed to decrease with decreasing concentration for both plants but that of O. gratissimum was lower when compared with that of T. occidentalis respectively. Conclusion: This pharmacological study is a useful tool for further drug development from the natural plant products.

Zawartość związków fenolowych w ekstrakcie z propolisu oraz ocena jego aktywności przeciwutleniającej i cytoochronnej względem erytrocytów ludzkich w warunkach stresu oksydacyjnego in vitro

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Marta Babicka ◽  
Elżbieta Hołderna-Kędzia ◽  
...  
Keyword(s):  
Free Radicals ◽  
Phenolic Acids ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Natural Material ◽  
Propolis Extract

Introduction. Propolis (bee glue) is a natural product collected by honeybees from buds of various trees, shrubs and other plant species. Extracts of propolis possess numerous biological activities, including antioxidant, antibacterial, antifungal and anticancer. For this reason, propolis is currently used in many applications, such as preparations for cold syndrome, dermatological preparations or as a constituent of nutritional supplements and health food. The chemical composition of this natural material is very complex and depending on many factors, including method of extraction and selection of the solvent for the extraction process. Aim. The aim of the study was to determine concentration of selected phenolic compounds (flavonoids and phenolic acids) in extract of Polish propolis and estimate its antioxidant activity and effect on human red blood cells. Material and methods. In the propolis extract was determined concentration of 14 flavonoids and 9 phenolic acids using ultra-performance liquid chromatography equipped with a photodiode detector and a triple quadrupole mass spectrometer. The antioxidant potential of propolis extract was evaluated applying DPPH˙ free radical scavenging activity assay and Fe3+ reducing power assay. Moreover, the cytotoxicity and cytoprotective potential of propolis extract was estimated using human erythrocytes in vitro. Results. The propolis extract contained high concentration of pinocembrin, galangin, chrysin, apigenin, kaempferol, coumaric acid and cinnamic acid. It exhibited also high antioxidant potential. The antiradical activity of examined propolis extract was equal to 75% approx. activity of both standard antioxidants used in the study, namely Trolox and BHT. The reducing power of extract was equal to 65% approx. of Trolox and 80% of BHT, respectively. The propolis extract had no hemolytic activity, moreover, effectively protected human erythrocytes against free radicals-induced damage in vitro. Conclusions. The results of this study indicate that the propolis extract of national origin is a rich source of flavonoids and phenolic acids. Therefore, the propolis extract possesses a high antioxidant potential and can protect erythrocytes against free radicals-induced oxidative hemolysis.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

scholarly journals Antioxidant Potential, Phytochemicals Composition, and Metal Contents of Datura alba

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Waliullah Khan ◽  
Sidra Subhan ◽  
Dilawar Farhan Shams ◽  
Sahib Gul Afridi ◽  
Riaz Ullah ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Radical Scavenging ◽  
Pharmaceutical Formulations ◽  
Antioxidant Defense System ◽  
Antioxidant Potential ◽  
Defense System ◽  
Metal Contents ◽  
Stem Extract ◽  
Free Radical Scavenging Assay

This study investigated the phytochemical characteristics and antioxidant activity in leaves, roots, stem, flower, and seed parts of Datura alba (D. alba). The study also assessed the heavy metal (Cr, Mn, Zn, and Cu) accumulation in each part of the plant. Among the phytochemicals, alkaloids were found only in leaves while tannins, flavonoids, and phenols were present in all parts of the plant. For antioxidant activity, free radical scavenging assay for 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed using ascorbic acid as the standard. Higher activity was shown by stem extract in methanol and leaf extract in n-hexane, ethyl acetate, and chloroform. Furthermore, all the target heavy metals were detected in all plant sections with the highest concentration of Zn in leaves and Cu in stem, root, flower, and seed. Due to stronger antioxidant potential and phytochemical composition, D. alba could prove as valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals.

scholarly journals Effect of the Solvent on Propolis Phenolic Profile and its Antifungal, Antioxidant, and In Vitro Cytoprotective Activity in Human Erythrocytes Under Oxidative Stress

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4266
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Patrycja Kwaśniewska-Sip ◽  
Agnieszka Waśkiewicz ◽  
Piotr Nowak ◽  
...  
Keyword(s):  
Free Radical ◽  
Antifungal Activity ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Phenolic Profile ◽  
Cytoprotective Activity

Propolis is a natural bee product with various beneficial biological effects. The health-promoting properties of propolis depend on its chemical composition, particularly the presence of phenolic compounds. The aim of this study was to evaluate the relationship between extraction solvent (acetone 100%, ethanol 70% and 96%) and the antifungal, antioxidant, and cytoprotective activity of the extracts obtained from propolis. Concentrations of flavonoids and phenolic acids in the propolis extracts were determined using ultrahigh-performance liquid chromatography. The antioxidant potential of different extracts was assessed on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free-radical-scavenging activity, Fe3+-reducing power, and ferrous ion (Fe2+)-chelating activity assays. The ability of the extracts to protect human red blood cell membranes against free-radical-induced damage and their antifungal activity was also determined. The results showed that the concentration of flavonoids in the propolis extracts was dependent on the solvent used in the extraction process and pinocembrin, chrysin, galangin, and coumaric acid were the most abundant phenols. All extracts exhibited high antioxidant potential and significantly protected human erythrocytes against oxidative damage. On the other hand, the antifungal activity of the propolis extracts depended on the solvent used in extraction and the fungal strains tested. It needs to be stressed that, to the best of our knowledge, there is no study relating the effect of solvent used for extraction of Polish propolis to its phenolic profile, and its antifungal, antioxidant, and cytoprotective activity.

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