scholarly journals Subpicosecond dynamics in DNA from leaves ofin vitro-grown apple plants: A SERS study

2011 ◽  
Vol 26 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu ◽  
Nicolae Leopold ◽  
Monica A. P. Purcaru
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Metallic Surface ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Subgroup ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Leaf Tissues ◽  
Genomic Dnas

In this work the SERS total half bandwidths of five genomic DNAs fromin vitro-grown apple leaf tissues (Malus domesticaBorkh., FamRosaceae, cvs. Florina, Rebra, Goldrush, Romus 3 and Romus 4) have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure, on the type of genomic apple plant DNA and on time, are reported. An example of the time-dependence of SERS band parameters of DNA, in the proximity of silver nanoparticles, has been given. It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs fromin vitro-grown apple leaf tissues can be monitored with surface-enhanced Raman spectroscopy. Particularly, the SERS band parameters for the vibrations near 757 cm–1(dT), 773 cm–1(dC), 929 cm–1(deoxyribose), 1087 cm–1(PO2–symmetric stretch, backbone), 1127 cm–1(dA), 1181 cm–1(ring vibrations of dG, dT, dC), 1311 cm–1(dA), 1362 cm–1(dT, dA), 1510 cm–1(dA), 1573 cm–1(dG, dA) and 1650 cm–1[dT(C═O), δ(H2O)] of genomic DNAs from apple leaves are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs fromin vitro-grown apple leaf tissues are typically in the wavenumber range from 14 to 52 cm–1. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.76 ps and larger than 0.20 ps. A comparison between different ranges of FT-Raman and SERS band parameters of DNA extracted from leaf tissues, respectively, is given. We have found that the bands of DNA from Romus 3 and Rebra cultivars are suitable for studying the dynamical behaviour of molecular subgroups, in genomic nucleic acids extracted fromin vitro-grown apple plants.

scholarly journals Molecular relaxation processes in genomic DNA from leaf tissues: A surface-enhanced Raman spectroscopic study

2011 ◽  
Vol 26 (4-5) ◽  
pp. 245-254 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu ◽  
Nicolae Leopold
Keyword(s):  
Genomic Dna ◽  
Metallic Surface ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Relaxation ◽  
Data Set ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Leaf Tissues ◽  
Genomic Dnas

In this work the SERS total half bandwidths of six genomic DNAs from ex vitro-grown apple leaf tissues (Malus domesticaBorkh., Fam. Rosaceae, cvs. Rebra, Goldrush, Florina, Romus 4, and the rootstocks M26, M9) and of five genomic DNAs fromin vitrogrown plants ofArnica montana(L.), Fam. Compositae,Astragalus peterfii(Jáv.), Fam. Fabaceae, strawberry (Duch.), Fam. Rosaceae, carnation (Dianthus caryophyllusL.), Fam. Caryophyllaceae, andKalanchoe x hybrida, Fam. Crassulaceae, respectively, have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure and on the type of genomic DNA, are reported.It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs from leaf tissues can be monitored with surface-enhanced Raman spectroscopy. In our study, the full widths at half-maximum (FWHMs) for the SERS bands of genomic DNAs from different leaf tissues, are typically in the wavenumber range from 15 to 30 cm–1for data set 1 and from 13 to 42 cm–1for data set 2. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.71 ps and larger than 0.35 ps (data set 1) and also varying between 0.25–0.82 ps for data set 2. A comparison between different ranges of FT-Raman and SERS band parameters, respectively, of DNA extracted from leaf tissues is given. We have found that the bands of DNA from Florina cultivar and Kalanchoe leaves, respectively, are suitable for the study of dynamical behaviour of molecular subgroups in nucleic acids extracted from different leaf tissues.

scholarly journals Surface-Enhanced Raman Spectroscopy for Cancer Immunotherapy Applications: Opportunities, Challenges, and Current Progress in Nanomaterial Strategies

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1145 ◽  
Author(s):  
Shuvashis Dey ◽  
Matt Trau ◽  
Kevin M. Koo
Keyword(s):  
Raman Spectroscopy ◽  
Cancer Immunotherapy ◽  
Immune Checkpoint ◽  
Checkpoint Inhibitors ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Checkpoint Receptors

Cancer immunotherapy encompasses a variety of approaches which target or use a patient’s immune system components to eliminate cancer. Notably, the current use of immune checkpoint inhibitors to target immune checkpoint receptors such as CTLA-4 or PD-1 has led to remarkable treatment responses in a variety of cancers. To predict cancer patients’ immunotherapy responses effectively and efficiently, multiplexed immunoassays have been shown to be advantageous in sensing multiple immunomarkers of the tumor microenvironment simultaneously for patient stratification. Surface-enhanced Raman spectroscopy (SERS) is well-regarded for its capabilities in multiplexed bioassays and has been increasingly demonstrated in cancer immunotherapy applications in recent years. This review focuses on SERS-active nanomaterials in the modern literature which have shown promise for enabling cancer patient-tailored immunotherapies, including multiplexed in vitro and in vivo immunomarker sensing and imaging, as well as immunotherapy drug screening and delivery.

scholarly journals Application of Doehlert Matrix for an Optimized Preparation of a Surface-Enhanced Raman Spectroscopy (SERS) Substrate Based on Silicon Nanowires for Ultrasensitive Detection of Rhodamine 6G

2019 ◽  
Vol 74 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Awatef Ouhibi ◽  
Maroua Saadaoui ◽  
Nathalie Lorrain ◽  
Mohammed Guendouz ◽  
Noureddine Raouafi ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nitrate ◽  
Silicon Nanowires ◽  
Rhodamine 6G ◽  
Surface Enhanced Raman Spectroscopy ◽  
Influential Factor ◽  
Metallic Surface ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

In this work, we combined a hierarchical nano-array effect of silicon nanowires (SiNWs) with a metallic surface of silver nanoparticles (AgNPs) to design a surface-enhanced Raman spectroscopy (SERS) scattering substrate for sensitive detection of Rhodamine 6G (R6G) which is a typical dye for fluorescence probes. The SiNWs were prepared by Metal-Assisted Chemical Etching (MACE) of n-Si (100) wafers. The Doehlert design methodology was used for planning the experiment and analyzing the experimental results. Thanks to this methodology, the R6G SERS response has been optimized by studying the effects of the silver nitrate concentration, silver nitrate and R6G immersion times and their interactions. The immersion time in R6G solution stands out as the most of influential factor on the SERS response.

Monitoring in vitro neural stem cell differentiation based on surface-enhanced Raman spectroscopy using a gold nanostar array

2015 ◽  
Vol 3 (16) ◽  
pp. 3848-3859 ◽  
Author(s):  
Waleed Ahmed El-Said ◽  
Seung U. Kim ◽  
Jeong-Woo Choi
Keyword(s):  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Differentiation ◽  
Surface Enhanced Raman Spectroscopy ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Cell Chip ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Neuro-cell chip was developed for non-invasive monitoring of neural stem cell stimulation using SERS technique that enabled the real-time monitoring, which is important for tissue development protocols.

scholarly journals FT-Raman study of the (sub)picosecond dynamics in genomic DNA from plant tissues

2009 ◽  
Vol 23 (5-6) ◽  
pp. 281-289 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu
Keyword(s):  
Relaxation Time ◽  
Genomic Dna ◽  
Vibrational Energy ◽  
Raman Band ◽  
Dynamical Behavior ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Relaxation ◽  
Leaf Tissues ◽  
Genomic Dnas

In this paper the Raman total half bandwidths of eight genomic DNAs from leaf tissues [potato (Solanum tuberosumL.), sword fern (Nephrolepis exaltataL.), scopolia (Scopolia carniolicaJacq.), redwood (Sequoia sempervirensD. Don. Endl.), orchids (Cymbidium × hybrida), chrysanthemum (Dendranthema grandifloraRamat.) and common sundew (Drosera rotundifoliaL.)] have been measured. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure and on the type of genomic plant DNA, are reported. It is shown that changes in the (sub)picosecond dynamics of molecular subgroups in genomic DNAs from leaf tissues can be monitored with Raman spectroscopy.Particularly, the Raman band parameters for the vibrations at 879 cm−1(deoxyribose, dA), 1047 cm−1(CO stretching C‒O‒P‒O‒C, dG), 1089 cm−1(P—O symmetric stretching of PO2‒), 1124 cm−1(dA), 1272 cm−1(dC, dG, dT), 1276 cm−1(dC), 1455 cm−1(deoxyribose, dA, dC, dT) and 1482 cm−1(dG, dA) of genomic leaf tissues DNAs are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs from leaf tissues are typically in the wavenumber range from 7.8 to 23.1 cm−1. It can be observed that the molecular relaxation processes studied in this work, have a global relaxation time smaller than 1.36 ps and larger than 0.46 ps.The fastest and the slowest relaxation processes of different DNA structural subgroups, for several types of genomic DNA extracted from leaf tissue, have been analyzed. Particularly, the slowest dynamics corresponding to the vibration near 1272 cm−1takes place in the case of DNA extracted from common sundew (global relaxation time 1.36 ps).A comparison between different time scales of the vibrational energy transfer processes, characterizing several DNA complexes, has been given.We have found that the bands at 879 cm−1(deoxyribose, phosphodiester, dA) and 1455 cm−1(deoxyribose, dA, dC, dT) are suitable for the study of dynamical behavior of molecular subgroups in genomic DNA extracted from leaf tissues.Specific molecular relaxation processes, depending on the type of genomic DNA extracted from leaf tissues has been observed.

scholarly journals Functionalized Gold Nanoparticles as Biosensors for Monitoring Cellular Uptake and Localization in Normal and Tumor Prostatic Cells

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 87 ◽  
Author(s):  
Marianna Pannico ◽  
Anna Calarco ◽  
Gianfranco Peluso ◽  
Pellegrino Musto
Keyword(s):  
Cellular Uptake ◽  
Colloidal Solution ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanospheres ◽  
Present Contribution ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Imaging

In the present contribution the fabrication and characterization of functionalized gold nanospheres of uniform shape and controlled size is reported. These nano-objects are intended to be used as Surface Enhanced Raman Spectroscopy (SERS) sensors for in-vitro cellular uptake and localization. Thiophenol was used as molecular reporter and was bound to the Au surface by a chemisorption process in aqueous solution. The obtained colloidal solution was highly stable and no aggregation of the single nanospheres into larger clusters was observed. The nanoparticles were incubated in human prostatic cells with the aim of developing a robust, SERS-based method to differentiate normal and tumor cell lines. SERS imaging experiments showed that tumor cells uptake considerably larger amounts of nanoparticles in comparison to normal cells (up to 950% more); significant differences were also observed in the uptake kinetics. This largely different behaviour might be exploited in diagnostic and therapeutic applications.

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