scholarly journals Molecular relaxation processes in genomic DNA from leaf tissues: A surface-enhanced Raman spectroscopic study

2011 ◽  
Vol 26 (4-5) ◽  
pp. 245-254 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu ◽  
Nicolae Leopold
Keyword(s):  
Genomic Dna ◽  
Metallic Surface ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Relaxation ◽  
Data Set ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Leaf Tissues ◽  
Genomic Dnas

In this work the SERS total half bandwidths of six genomic DNAs from ex vitro-grown apple leaf tissues (Malus domesticaBorkh., Fam. Rosaceae, cvs. Rebra, Goldrush, Florina, Romus 4, and the rootstocks M26, M9) and of five genomic DNAs fromin vitrogrown plants ofArnica montana(L.), Fam. Compositae,Astragalus peterfii(Jáv.), Fam. Fabaceae, strawberry (Duch.), Fam. Rosaceae, carnation (Dianthus caryophyllusL.), Fam. Caryophyllaceae, andKalanchoe x hybrida, Fam. Crassulaceae, respectively, have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure and on the type of genomic DNA, are reported.It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs from leaf tissues can be monitored with surface-enhanced Raman spectroscopy. In our study, the full widths at half-maximum (FWHMs) for the SERS bands of genomic DNAs from different leaf tissues, are typically in the wavenumber range from 15 to 30 cm–1for data set 1 and from 13 to 42 cm–1for data set 2. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.71 ps and larger than 0.35 ps (data set 1) and also varying between 0.25–0.82 ps for data set 2. A comparison between different ranges of FT-Raman and SERS band parameters, respectively, of DNA extracted from leaf tissues is given. We have found that the bands of DNA from Florina cultivar and Kalanchoe leaves, respectively, are suitable for the study of dynamical behaviour of molecular subgroups in nucleic acids extracted from different leaf tissues.

scholarly journals Subpicosecond dynamics in DNA from leaves ofin vitro-grown apple plants: A SERS study

2011 ◽  
Vol 26 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu ◽  
Nicolae Leopold ◽  
Monica A. P. Purcaru
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Metallic Surface ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Subgroup ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Leaf Tissues ◽  
Genomic Dnas

In this work the SERS total half bandwidths of five genomic DNAs fromin vitro-grown apple leaf tissues (Malus domesticaBorkh., FamRosaceae, cvs. Florina, Rebra, Goldrush, Romus 3 and Romus 4) have been measured. We have shown that surface-enhanced Raman scattering can be used to study the fast subpicosecond dynamics of DNA in the proximity of a metallic surface. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure, on the type of genomic apple plant DNA and on time, are reported. An example of the time-dependence of SERS band parameters of DNA, in the proximity of silver nanoparticles, has been given. It is shown that changes in the subpicosecond surface dynamics of molecular subgroups in genomic DNAs fromin vitro-grown apple leaf tissues can be monitored with surface-enhanced Raman spectroscopy. Particularly, the SERS band parameters for the vibrations near 757 cm–1(dT), 773 cm–1(dC), 929 cm–1(deoxyribose), 1087 cm–1(PO2–symmetric stretch, backbone), 1127 cm–1(dA), 1181 cm–1(ring vibrations of dG, dT, dC), 1311 cm–1(dA), 1362 cm–1(dT, dA), 1510 cm–1(dA), 1573 cm–1(dG, dA) and 1650 cm–1[dT(C═O), δ(H2O)] of genomic DNAs from apple leaves are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs fromin vitro-grown apple leaf tissues are typically in the wavenumber range from 14 to 52 cm–1. Besides, it can be observed that molecular relaxation processes studied in this work, have a global relaxation time smaller than 0.76 ps and larger than 0.20 ps. A comparison between different ranges of FT-Raman and SERS band parameters of DNA extracted from leaf tissues, respectively, is given. We have found that the bands of DNA from Romus 3 and Rebra cultivars are suitable for studying the dynamical behaviour of molecular subgroups, in genomic nucleic acids extracted fromin vitro-grown apple plants.

scholarly journals FT-Raman study of the (sub)picosecond dynamics in genomic DNA from plant tissues

2009 ◽  
Vol 23 (5-6) ◽  
pp. 281-289 ◽  
Author(s):  
Cristina M. Muntean ◽  
Ioan Bratu
Keyword(s):  
Relaxation Time ◽  
Genomic Dna ◽  
Vibrational Energy ◽  
Raman Band ◽  
Dynamical Behavior ◽  
Relaxation Processes ◽  
Molecular Subgroups ◽  
Molecular Relaxation ◽  
Leaf Tissues ◽  
Genomic Dnas

In this paper the Raman total half bandwidths of eight genomic DNAs from leaf tissues [potato (Solanum tuberosumL.), sword fern (Nephrolepis exaltataL.), scopolia (Scopolia carniolicaJacq.), redwood (Sequoia sempervirensD. Don. Endl.), orchids (Cymbidium × hybrida), chrysanthemum (Dendranthema grandifloraRamat.) and common sundew (Drosera rotundifoliaL.)] have been measured. The dependencies of the total half bandwidths and of the global relaxation times, on DNA molecular subgroup structure and on the type of genomic plant DNA, are reported. It is shown that changes in the (sub)picosecond dynamics of molecular subgroups in genomic DNAs from leaf tissues can be monitored with Raman spectroscopy.Particularly, the Raman band parameters for the vibrations at 879 cm−1(deoxyribose, dA), 1047 cm−1(CO stretching C‒O‒P‒O‒C, dG), 1089 cm−1(P—O symmetric stretching of PO2‒), 1124 cm−1(dA), 1272 cm−1(dC, dG, dT), 1276 cm−1(dC), 1455 cm−1(deoxyribose, dA, dC, dT) and 1482 cm−1(dG, dA) of genomic leaf tissues DNAs are presented. In our study, the full widths at half-maximum (FWHM) of the bands in genomic DNAs from leaf tissues are typically in the wavenumber range from 7.8 to 23.1 cm−1. It can be observed that the molecular relaxation processes studied in this work, have a global relaxation time smaller than 1.36 ps and larger than 0.46 ps.The fastest and the slowest relaxation processes of different DNA structural subgroups, for several types of genomic DNA extracted from leaf tissue, have been analyzed. Particularly, the slowest dynamics corresponding to the vibration near 1272 cm−1takes place in the case of DNA extracted from common sundew (global relaxation time 1.36 ps).A comparison between different time scales of the vibrational energy transfer processes, characterizing several DNA complexes, has been given.We have found that the bands at 879 cm−1(deoxyribose, phosphodiester, dA) and 1455 cm−1(deoxyribose, dA, dC, dT) are suitable for the study of dynamical behavior of molecular subgroups in genomic DNA extracted from leaf tissues.Specific molecular relaxation processes, depending on the type of genomic DNA extracted from leaf tissues has been observed.

Analysis of Genomic DNAs from Nine Rosaceae Species Using Surface-Enhanced Raman Scattering

2015 ◽  
Vol 69 (12) ◽  
pp. 1487-1495 ◽  
Author(s):  
Qiu Lu ◽  
Tao Lang ◽  
Shuguo Fan ◽  
Wen Chen ◽  
Deqing Zang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Rosaceae Species ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Genomic Dnas

scholarly journals Application of Doehlert Matrix for an Optimized Preparation of a Surface-Enhanced Raman Spectroscopy (SERS) Substrate Based on Silicon Nanowires for Ultrasensitive Detection of Rhodamine 6G

2019 ◽  
Vol 74 (2) ◽  
pp. 168-177 ◽  
Author(s):  
Awatef Ouhibi ◽  
Maroua Saadaoui ◽  
Nathalie Lorrain ◽  
Mohammed Guendouz ◽  
Noureddine Raouafi ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nitrate ◽  
Silicon Nanowires ◽  
Rhodamine 6G ◽  
Surface Enhanced Raman Spectroscopy ◽  
Influential Factor ◽  
Metallic Surface ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

In this work, we combined a hierarchical nano-array effect of silicon nanowires (SiNWs) with a metallic surface of silver nanoparticles (AgNPs) to design a surface-enhanced Raman spectroscopy (SERS) scattering substrate for sensitive detection of Rhodamine 6G (R6G) which is a typical dye for fluorescence probes. The SiNWs were prepared by Metal-Assisted Chemical Etching (MACE) of n-Si (100) wafers. The Doehlert design methodology was used for planning the experiment and analyzing the experimental results. Thanks to this methodology, the R6G SERS response has been optimized by studying the effects of the silver nitrate concentration, silver nitrate and R6G immersion times and their interactions. The immersion time in R6G solution stands out as the most of influential factor on the SERS response.

scholarly journals Surface-enhanced Raman spectroscopy for the characterization of Vaccinium myrtillus L. bilberries of the Baltic–Nordic regions

2021 ◽  
Author(s):  
Lina Traksele ◽  
Valentinas Snitka
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Principal Component ◽  
Surface Enhanced Raman Spectroscopy ◽  
Vaccinium Myrtillus ◽  
Spectral Variation ◽  
Data Set ◽  
Sers Substrates ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractIn this study, an investigation of the wild bilberries (Vaccinium myrtillus L.) of the different Baltic–Nordic regions using surface-enhanced Raman spectroscopy (SERS) combined with principal component analysis (PCA) is presented. The bilberries were collected in Lithuania, Latvia, Finland and Norway. The set of the SERS spectra of the berry extracts (pH ~ 4) were recorded on the silver nanoparticles based SERS substrates. The SERS spectra of the extracts were acquired using 532 nm laser as an excitation source. The morphology of the SERS substrates was evaluated by scanning electron microscopy (SEM) and the presence of the silver nanoparticles was confirmed by the energy-dispersive X-ray spectroscopy (EDX). The enhancement factor (EF) of the silver SERS substrates was found to be 105. It has been shown that a strong fluorescence background, associated with the phenolic compounds found in bilberries, can be subtracted due to the fluorescence-quenching properties of the silver nanoparticles. Therefore, an application of the SERS technique allowed to observe the characteristic peaks of the bilberries and the PCA tool enabled to evaluate the spectral variation across the entire SERS data set. The results presented in this paper show that the SERS technique coupled with PCA chemometric analysis might serve as a complementary method that allows to identify the country of origin of the bilberries based on the spectral differences.

scholarly journals Ultrasensitive detection of genomic DNA from apple leaf tissues, using surface-enhanced Raman scattering

2011 ◽  
Vol 25 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Cristina M. Muntean ◽  
Nicolae Leopold ◽  
Adela Halmagyi ◽  
Sergiu Valimareanu
Keyword(s):  
Raman Scattering ◽  
Nucleic Acids ◽  
Structural Changes ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ultrasensitive Detection ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Genomic Dnas

Ultrasensitive detection of nucleic acids is demonstrated through exploiting the effect of surface-enhanced Raman scattering (SERS). In this work the SERS spectra of eight genomic DNAs from leaves of apple trees grown in the field (Malus domesticaBorkh., Fam.Rosaceae, cultivars. Florina, Idared, Rebra, Goldrush, Romus 3, Romus 4 and the rootstocks M9 and M26) have been analyzed in the wavenumber range 200–1800 cm–1. SERS signatures, spectroscopic band assignments and structural interpretations of these plant genomic DNAs are reported. SERS spectra of nucleic acids are compared here with caution, because these signals are time-dependent and are strongly influenced by DNA amount in the measured sample volume. Similarities of the SERS spectra of genomic DNAs extracted from apple leaves of Goldrush, Rebra and Florina cultivars, respectively, have been detected. Besides, the SERS spectra, corresponding to DNA from M9, Romus 4 and M26, leaves, respectively, show similar features and well resolved bands. Based on this work, specific plant DNA-ligand interactions or DNA structural changes induced by plant stress conditions associated with their natural environment, might be further investigated using surface-enhanced Raman spectroscopy.

scholarly journals Raman Spectroscopy in the Analysis of Textile Structures

2021 ◽  
Author(s):  
Dorota Puchowicz ◽  
Malgorzata Cieslak
Keyword(s):  
Raman Spectroscopy ◽  
Natural Fibers ◽  
Surface Enhanced Raman Spectroscopy ◽  
Metallic Surface ◽  
Textile Materials ◽  
Synthetic Fibers ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Non Destructive

Raman spectroscopy as a non-destructive technique is very often used to analyze a historic or forensic material. It is also a very valuable method of testing textile materials, especially modified and functionalized. In the case of textiles, the advantages of this technique is the compatibility inter alia with FTIR, which is helpful in natural fibers identification or to distinguish between isomers and conformers of synthetic fibers. The work shows the possibility of special application of the Raman spectroscopy to the characterization of textile materials after modification and functionalization with nanoparticles. A functionalized textile structure with a metallic surface can provide a good basis for analytical studies using surface enhanced Raman spectroscopy as it was presented on the example of wool, cotton and aramid fibers.

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