scholarly journals Spectrophometric Determination of Nelfinavir Mesylate

2006 ◽  
Vol 3 (2) ◽  
pp. 78-82 ◽  
Author(s):  
K. Vanitha Prakash ◽  
Jangala Venkateswara Rao
Keyword(s):  
Ferric Chloride ◽  
Dosage Form ◽  
Potassium Ferricyanide ◽  
Reagent Blank ◽  
Spectrophotometric Methods ◽  
Bluish Green ◽  
Nelfinavir Mesylate ◽  
Bulk Drug ◽  
Tablet Dosage

Two new simple, sensitive, rapid and economical Spectrophotometric Methods (A and B) have been developed for the determination of Nelfinavir Mesylate in pharmaceutical bulk and tablet dosage form. The method A is based on the reaction of Nelfinavir with ferric chloride, potassium ferricyanide and hydrochloric acid to form a bluish green colored chromogen. The Method B is based on the formation of blood red colored chromogen with Ferric chloride and 1,10-phenanthroline. The absorbances of the chromogen were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

scholarly journals Spectrophotometric Determination of Raloxifene Hydrochloride in Pharmaceutical Formulations

2007 ◽  
Vol 4 (1) ◽  
pp. 79-82 ◽  
Author(s):  
M. Mathrusri Annapurna ◽  
M. E. Bhanoji Rao ◽  
B. V. V. Ravi Kumar
Keyword(s):  
Ferric Chloride ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Reagent Blank ◽  
Spectrophotometric Methods ◽  
Maximum Absorbance ◽  
Bulk Drug ◽  
Raloxifene Hydrochloride ◽  
Tablet Dosage

Three new simple, sensitive, rapid and economical spectrophotometric Methods (A, B and C) have been developed for the determination of Raloxifene Hydrochloride in pharmaceutical bulk and tablet dosage form. Method A is based on the formation of yellow colored chromogen with 0.1N Sodium hydroxide exhibiting maximum absorbance against the corresponding reagent blank. The method B is based on the reaction of Raloxifene with Ferric chloride and 1, 10-phenanthroline to form blood red colored chromogen. The method C is based on the formation of blood red colored chromogen with Ferric chloride and 2, 2' bipyridyl. The absorbencies of the chromogens were measured at their respective wavelength of maximum absorbance against the corresponding reagent blank. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate.

scholarly journals Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

2012 ◽  
Vol 9 (1) ◽  
pp. 89-92
Author(s):  
J. Sudhakar Reddy ◽  
MD. S. Maqsood Ahmed ◽  
I. E. Chakravarthy ◽  
K. Prabhavathi
Keyword(s):  
Statistical Analysis ◽  
Dosage Form ◽  
Dosage Forms ◽  
Ion Pair ◽  
Reagent Blank ◽  
Pharmaceutical Dosage Forms ◽  
Extractable Complex ◽  
Bulk Drug ◽  
Tablet Dosage

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

scholarly journals Permanganometric determination of etamsylate in bulk drug and in tablets

2009 ◽  
Vol 15 (3) ◽  
pp. 149-157 ◽  
Author(s):  
K.B. Vinay ◽  
H.D. Revanasiddappa ◽  
Zenita Okram ◽  
Kanakapura Basavaiah
Keyword(s):  
Molar Absorptivity ◽  
Molar Ratio ◽  
Spectrophotometric Methods ◽  
Fixed Concentration ◽  
Bluish Green ◽  
Bulk Drug ◽  
Direct Spectrophotometry ◽  
Assay Conditions ◽  
Drug Solution

One titrimetric and two spectrophotometric methods which are simple, selective, sensitive, accurate, precise and economical for the determination of etamsylate (ETM) in bulk drug and in tablets employing permanganate as the oxidimetric reagent are described. In titrimetry, ETM is titrated directly with permanganate in sulphuric acid medium. A direct spectrophotometry (method A) involves treating the aqueous solution of the drug with permanganate in alkaline medium and measuring the bluish green product at 610 nm. In indirect spectrophotometry (method B), the drug solution was treated with a fixed concentration of permanganate in H2SO4 medium, and after a specified time, the unreacted permanganate was measured at 545 nm. The molar combining ratio in titrimetry and the optimum assay conditions were studied. Titrimetry is applicable over 1-10 mg range and the calculations are based on a 1:4 (ETM:KMnO4) molar ratio. In spectrophotometry, Beer's law is obeyed over 0.5- 5.0 and 1.5-15 ?g ml-1 for method A and B, respectively. The molar absorptivity values are calculated to be 2.79?104 and 4.17?104 l mol-1 cm-1 for method A and B, respectively and the corresponding sandell sensitivity values are 0.0094 and 0.0063 ?g cm-2. The limits of detection (LOD) and quantification (LOQ) are also reported for spectrophotometric methods. The applicability of the developed methods was demonstrated by the determination of etamsylate in pure drug as well as in commercial dosage forms.

scholarly journals Two new spectrophotometric methods for the determination of isoniazid in bulk form and tablet dosage form

2019 ◽  
Vol 57 (3) ◽  
pp. 117
Author(s):  
Olajire Adegoke ◽  
Olusegun Thomas ◽  
Deborah Babatunde ◽  
Oyindamola Oyelami ◽  
Adeyinka Adediran ◽  
...  
Keyword(s):  
Dosage Form ◽  
Spectrophotometric Methods ◽  
Tablet Dosage

Determination of nelfinavir mesylate as bulk drug and in pharmaceutical dosage form by stability indicating HPLC

2006 ◽  
Vol 41 (3) ◽  
pp. 1065-1069 ◽  
Author(s):  
Qiufang Jing ◽  
Yongjia Shen ◽  
Yanhui Tang ◽  
Fuzheng Ren ◽  
Xinhong Yu ◽  
...  
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Nelfinavir Mesylate ◽  
Bulk Drug

scholarly journals Sensitive spectrophotometric assay of simvastatin in pharmaceuticals using permanganate

2010 ◽  
Vol 46 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Kalsang Tharpa ◽  
Kanakapura Basavaiah ◽  
Nagaraju Rajedraprasad ◽  
Kanakapura Basavaiah Vinay ◽  
Salmara Ganeshbhat Hiriyanna
Keyword(s):  
Optimum Conditions ◽  
Acetic Acid Medium ◽  
Recovery Method ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Ich Guidelines ◽  
Bulk Drug ◽  
Tablet Dosage ◽  
Better Than

Two simple, sensitive, selective and inexpensive spectrophotometric methods are described for the determination of simvastatin (SMT) in bulk drug and in tablets using permanganate as the oxidimetric reagent. In method A, SMT is treated with a measured excess of permanganate in acetic acid medium and the unreacted oxidant is measured at 550 nm, whereas in method B the reaction is carried out in alkaline medium and the resulting manganate is measured at 610 nm. In method A, the amount of permanganate reacted corresponds to the SMT content and the absorbance is found to decrease linearly with the concentration; and in method B, the absorbance increases with concentration. The working conditions of assays were optimized, and the methods were validated according to the current ICH guidelines. Under optimum conditions, SMT could be assayed in the concentration ranges, 1.47 - 17.67x10-5 and 2.27 - 27.18 x10-6 mol/L by method A and method B, respectively. The calculated molar absorptivities are 3.2 x 10³ and 2.5 x 10(4) L/mol/cm for method A and method B, respectively with corresponding Sandell sensitivity values of 0.0387 and 0.0178 μg/cm². The limits of detection (LOD) and quantification (LOQ) have also been reported. Accuracy and precision for the assay were determined by calculating the intra-day and inter-day at three concentrations; the intra-day RSD was < 2% and the accuracy was better than 2.15 % (RE). The methods were applied successfully for the determination of SMT in tablet dosage form with a high percentage of recovery, good accuracy and precision, and without measurable interference by the excipients. The accuracy was further ascertained from placebo and synthetic mixture analysis and also from the spike-recovery method.

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