Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

Spectrophotometric Estimation of Abacavir Sulphate in Pharmaceutical Formulations

Two simple, accurate, rapid and sensitive methods (A and B) have been developed for the estimation of abacavir sulphate in its pharmaceutical dosage form. The method A and B are based on the formation of chloroform extractable complex of abacavir sulphate with bromophenol blue (method A) and bromocresol green (method B), which shows absorbance maxima at 460 nm and 469 nm respectively. The absorbance-concentration plot is linear over the range of 1-10 mcg/mL for method A and B respectively. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods are economical and sensitive for the estimation of abacavir sulphate in bulk drug and in its tablet dosage form.

Rapid Spectrophotometric Method for Determination of 2-Aminobutane in Postharvest Dips

A rapid method is described for measuring 2-aminobutane in postharvest dip fluids. Samples of the dip fluid are diluted to bring the 2-aminobutane content in the range 500—5000 μg and reacted with carbon disulfide and cupric ion to form a chloroform-extractable complex measured spectrophotometrically at 430 nm. The accuracy and reproducibility of the method are comparable with the more lengthy modified product and residue methods.