Stability indicating high-performance thin-layer chromatographic determination of nelfinavir mesylate as bulk drug and in pharmaceutical dosage form

2004 ◽  
Vol 502 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Neeraj Kaul ◽  
Himani Agrawal ◽  
A.R Paradkar ◽  
K.R Mahadik
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Nelfinavir Mesylate ◽  
Bulk Drug

High-performance thin-layer chromatographic determination of etoricoxib in the bulk drug and in pharmaceutical dosage form

2007 ◽  
Vol 20 (5) ◽  
pp. 335-339 ◽  
Author(s):  
Gourab Maheshwari ◽  
Ganesa Subramanian ◽  
Arumugam Karthik ◽  
Averineni Ranjithkumar ◽  
Prashant Musmade ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination ◽  
Pharmaceutical Dosage Form ◽  
Bulk Drug

scholarly journals Stability-Indicating High-Performance Thin-Layer Chromatographic Method for Quantitative Estimation of Emtricitabine in Bulk Drug and Pharmaceutical Dosage Form

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Atul S. Rathore ◽  
Lohidasan Sathiyanarayanan ◽  
Kakasaheb R. Mahadik
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Linear Regression Analysis ◽  
Solvent System ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Limit Of Quantitation ◽  
Bulk Drug

A simple, sensitive, precise, specific and stability indicating high-performance thin-layer chromatographic (HPTLC) method for the determination of emtricitabine both in bulk drug and pharmaceutical dosage form was developed and validated. The method employed aluminium plates precoated with silica gel G60 F254 as the stationary phase. The solvent system consisted of toluene : ethyl acetate : methanol (2 : 8 : 1, v/v/v). This solvent system was found to give compact spots for emtricitabine with value . Densitometric analysis of emtricitabine was carried out in the absorbance mode at 284 nm. Linear regression analysis showed good linearity with respect to peak area in the concentration range of 30–110 ng spot−1. The method was validated for precision, limit of detection (LOD), limit of quantitation (LOQ), robustness, accuracy and specificity. Emtricitabine was subjected to acid and alkali hydrolysis, oxidation, neutral hydrolysis, photodegradation and dry heat treatment. Also the degraded products peaks were well resolved from the pure drug with significantly different values. Statistical analysis proved that the method is repeatable and specific for the estimation of the said drug. As the method could effectively separate the drugs from their degradation products, it can be employed as a stability indicating method.

Stability indicating high-performance thin-layer chromatographic determination of gatifloxacin as bulk drug and from polymeric nanoparticles

2006 ◽  
Vol 576 (2) ◽  
pp. 253-260 ◽  
Author(s):  
Sanjay K. Motwani ◽  
Roop K. Khar ◽  
Farhan J. Ahmad ◽  
Shruti Chopra ◽  
K. Kohli ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Polymeric Nanoparticles ◽  
Chromatographic Determination ◽  
Stability Indicating ◽  
Bulk Drug

Determination of nelfinavir mesylate as bulk drug and in pharmaceutical dosage form by stability indicating HPLC

2006 ◽  
Vol 41 (3) ◽  
pp. 1065-1069 ◽  
Author(s):  
Qiufang Jing ◽  
Yongjia Shen ◽  
Yanhui Tang ◽  
Fuzheng Ren ◽  
Xinhong Yu ◽  
...  
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Nelfinavir Mesylate ◽  
Bulk Drug

scholarly journals STABILITY-INDICATING HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF FORMOTEROL FUMARATE DIHYDRATE AND FLUTICASONE PROPIONATE IN BULK DRUG AND PHARMACEUTICAL DOSAGE FORM

2019 ◽  
pp. 149-155
Author(s):  
RAJASHI B PHARATE ◽  
SUNEELA S DHANESHWAR
Keyword(s):  
Fluticasone Propionate ◽  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Bulk Drug ◽  
Formoterol Fumarate

Objective: The objective of the present work was to develop validated stability-indicating high-performance thin-layer chromatographic method for simultaneous estimation of formoterol fumarate dihydrate (FFD) and fluticasone propionate (FP) in bulk drug and pharmaceutical dosage form. Methods: Pre-coated silica gel aluminum plates 60 F-254 were used as stationary phase. The mixture of toluene:ethyl acetate:formic acid (98%) (6:4:0.1; v/v/v) was used as a mobile phase. The densitometric quantification was carried out at 233 nm. The method was validated according to the ICH guidelines. The specificity and stability indicating the capability of the method were proven though degradation studies. Both drugs were subjected to acid (0.1N HCl) and base (0.1N NaOH) hydrolysis, oxidation (3% v/v H2O2), photolytic, and neutral degradation conditions. Results: The selected mobile phase resolved peaks of FFD and FP with Rf values 0.27±0.10 and 0.64±0.10, respectively. Determination coefficients of calibration curves were found to be 0.998 and 0.999 in the range of 1–3.5 μg/spot and 10–60 μg/spot for FFD and FP with an accuracy of 99.09% for FFD and 99.20% for FP. The degradation products of FFD and FP were resolved from the pure drug with significant differences in their retention factor values. Conclusion: The developed method is simple, accurate and can be successfully applied for quantification of FFD and FP in bulk drug and pharmaceutical dosage form, contributing to improve the quality control and assure the therapeutic efficacy.

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