scholarly journals Comparison of Endoscopy-Based and Serum-Based Methods for the Diagnosis ofHelicobacter pylori

2003 ◽  
Vol 17 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Elvira Garza-González ◽  
Francisco J Bosques-Padilla ◽  
Rolando Tijerina-Menchaca ◽  
Juan P Flores-Gutiérrez ◽  
Héctor J Maldonado-Garza ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Diagnostic Value ◽  
The Body ◽  
Rapid Urease Test ◽  
Igg Antibodies ◽  
Ulcer Disease ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori ◽  
Age Range

Available commercial tests for the diagnosis ofHelicobacter pyloriinfection are based on different types of antigen preparations and hence the diagnostic utility differs substantially.OBJECTIVE: To assess the diagnostic value of the determination of Immunoglobulin (Ig) A and IgG antibodies toH pyloriwhole cell (WC) and IgG antibodies to cytotoxin associated gene A (CagA) using an in-house ELISA in relation to the results obtained with different invasive methods.METHODS: The study population consisted of 251 Mexican adults, mean age 53 years, age range 15 to 92 years and female to male ratio of 1.5. Peptic ulcer disease was present in 10.8% of these patients, 5.2% had gastric cancer, 11.2% had esophagitis and 72.9% had nonulcer dyspepsia. Biopsy specimens from the body and the antrum of the stomach were obtained for culture, histology and rapid urease test. ELISAs to detect IgA and IgG WC andCagAantibodies were performed using serum.RESULTS:H pyloristatus was established by the results of the invasive tests. Eighty (31.9%) patients positive to the three tests and 38 (15.1%) negative to all the tests were identified. Based on this result, the sensitivity and specificity of the serology assays were 97.5% and 78.9% for the IgG WC and 70% and 73.7% for the IgA WC, respectively. However, ifH pyloristatus was defined by the positive result of at least one or two invasive diagnostic tests, the sensitivity for the IgG WC decreased to 87.3% and 66.7% respectively, but the specificity was essentially the same. Similar results were obtained for the sensitivity and specificity of IgA using the same criteria. A lowCagAprevalence was observed (39%).CONCLUSIONS: Testing for serological IgG antibodies toH pyloriWC was the best to assess whether infection byH pyloriwas present. Neither the IgA WC nor the IgGCagAELISAs add significant value in the diagnosis ofH pylori.

scholarly journals Prospective study to evaluate the number and the location of biopsies in rapid urease test for diagnosis of Helicobacter Pylori

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Antoine Abou Rached ◽  
Jowana Saba ◽  
Cesar Yaghi ◽  
Joyce Sanyour ◽  
Ahmad El Hajjar ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Rapid Test ◽  
Rapid Urease Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Antral Biopsy

Helicobacter pylori (H. pylori) can cause a wide variety of illnesses such as peptic ulcer disease, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. The diagnosis and eradication of H. pylori are crucial. The diagnosis of H. pylori is usually based on the rapid urease test (RUT) and gastric antral biopsy for histology. The aim of this study is to evaluate the numbers of needed biopsies and their location (antrum/fundus) to obtain optimal result for the diagnosis of H. pylori. Three hundred fifty consecutive patients were recruited, 210 fulfill the inclusion criteria and had nine gastric biopsies for the detection of H. pylori infection: two antral for the first RUT (RUT1), one antral and one fundic for the second (RUT2), one antral for the third (RUT3) and two antral with two fundic for histology (HES, Giemsa, PAS). The reading of the 3 types of RUT was performed at 1 hour, 3 hours and 24 hours and biopsies were read by two experienced pathologists not informed about the result of RUT. Results of RUT were considered positive if H. pylori was found on histology of at least one biopsy. The RUT1 at 1h, 3h and 24h has a sensitivity of 72%, 82% and 89% and a specificity of 100%, 99% and 87% respectively. The positive predictive value (PPV) was 100%, 99% and 85% respectively and the negative predictive value (NPV) of 81%, 87% and 90%. The RUT2 at 1h, 3h and 24h, respectively, had a sensitivity of 86%, 87% and 91% and a specificity of 99%, 97% and 90%. The PPV was 99%, 96% and 88% and NPV of 89%, 90%, 94%. The RUT3 at 1h, 3h and 24h, respectively, had a sensitivity of 70%, 74% and 84% and a specificity of 99%, 99% and 94%. The PPV was 99%, 99% and 92% and NPV of 79%, 81% and 87%. The best sensitivity and specificity were obtained for RUT1 read at 3h, for RUT2 read 1h and 3h, and the RUT3 read at 24h.This study demonstrates that the best sensitivity and specificity of rapid test for urease is obtained when fundic plus antral biopsy specimens are used with a reading time at 3 hours.

scholarly journals A PROSPECTIVE, OBSERVATIONAL STUDY ON THE EFFICACY OF DRUG REGIMEN USED FOR HELICOBACTER PYLORI ERADICATION IN PEPTIC ULCER DISEASE

2021 ◽  
Vol 12 (6) ◽  
pp. 40-47
Author(s):  
Abdullah . ◽  
Mounika Pamukuntla
Keyword(s):  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Drug Regimen ◽  
The Body ◽  
Rapid Urease Test ◽  
Medication History ◽  
Patient Counselling ◽  
Ulcer Disease ◽  
Urease Test ◽  
H Pylori

Aim of the study: We aimed to study the efficacy of triple drug therapy which is considered as a standard regimen. Objective of the study: To observe the role of pharmacist in preventing the recurrence of H. pylori infection. To assess whether the drug regimen prescribed eradicates the H. pylori infection. Results: In a period of 6 months a total of 358 cases were observed. Among them 142 cases are rapid urease test +ve and 216 are rapid urease test – ve cases. Of 142 patients included in the analysis, Peptic ulcer disease was more common in men when compared to women and prevalent in age group of 51-60 years. The body mass index analysis showed that 35.2% of patients were obese. From the personal history of the patients, it was concluded that 32.3% were smokers whereas 31.6% were alcoholic. The medication history analysis showed non-steroidal anti-inflammatory drugs were commonly used by the patients. The diagnostic endoscopic reports show that patients suffer from erosive pan gastritis followed by erosive gastritis. Conclusion: A 14-day triple therapy was found to be completely eradicate H. pylori infection. Out of the various demographic details that were taken into consideration age, gender, BMI, smokers, alcoholics, spicy food intake and drug abuse increased symptoms whereas co-morbid illness, sleeping pattern and food interval didn’t affect much. Structured patient counselling and follow up had a significant effect which was seen in the form of zero recurrence, 100% medication adherence and improved quality of life.

scholarly journals Clinical Validation of an Office-Based14C-UBT (Heliprobe) forH. pyloriDiagnosis in Iranian Dyspeptic Patients

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Omid Sanaei ◽  
Farahnaz Joukar
Keyword(s):  
Predictive Value ◽  
Gold Standard ◽  
Urea Breath Test ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
Clinical Validation ◽  
Igg Antibodies ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background. We encountered repeatedly, in our clinical practice, discordant results between UBT and histopathology aboutH. pyloriinfection.Goal. To study the diagnostic accuracy of Heliprobe14C-urea breath test (14C-UBT) for detection ofH. pyloriinfection in an Iranian population.Study. We enrolled 125 dyspeptic patients in our study. All of them underwent gastroscopy, and four gastric biopsies (three from the antrum and one from the corpus) were obtained. One of the antral biopsies was utilized for a rapid urease test (RUT), and three others were evaluated under microscopic examination. Sera from all patients were investigated for the presence ofH. pyloriIgG antibodies. The14C-UBT was performed on all subjects using Heliprobe kit, and results were analyzed against the following gold standard (GS):H. pyloriinfection considered positive when any two of three diagnostic methods (histopathology, RUT, serology) are positive.Results. According to data analysis, the Heliprobe14C-UBT had 94% sensitivity, 100% specificity, 93% negative predictive value (NPV), 100% positive predictive value (PPV), and 97% accuracy, compared with GS.Conclusion. The Heliprobe14C-UBT is an easy-to-perform, rapid-response, and accurate test forH. pyloridiagnosis, suitable for office use.

scholarly journals Low Prevalence ofHelicobacter PyloriInfection in Canadian Children: A Cross-Sectional Analysis

2008 ◽  
Vol 22 (5) ◽  
pp. 485-489 ◽  
Author(s):  
Idit Segal ◽  
Anthony Otley ◽  
Robert Issenman ◽  
David Armstrong ◽  
Victor Espinosa ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Breath Test ◽  
Tertiary Care ◽  
Urea Breath Test ◽  
Rapid Urease Test ◽  
Infection Rates ◽  
Upper Gi ◽  
Gi Symptoms ◽  
Urease Test ◽  
H Pylori

BACKGROUND: The incidence and prevalence rates of childhoodHelicobacter pyloriinfection vary greatly by nation, with infection rates of 8.9% to 72.8% reported in developed and developing countries, respectively. To date, few studies have assessed the prevalence ofH pyloriin Canadian children, with studies limited to Aboriginal communities and single tertiary care centres from Ontario and Quebec.OBJECTIVES: To determine the prevalence ofH pyloriin consecutive children referred to three Canadian tertiary care academic centres for upper gastrointestinal (GI) endoscopy due to upper GI symptoms, and to determine the sensitivity and specificity of the carbon-13-labelled urea breath test, the rapid urease test and theH pyloristool monoclonal antigen test.RESULTS: Two hundred four patients were recruited. The prevalence ofH pyloriwas 7.1%. Of theH pylori-positive patients, 41.7% were male, with a mean age of 10.3 years. Ethnic minorities accounted for 42% of theH pylori-positive patients. Consistent with previous observations, the sensitivity and specificity of the carbon-13-labelled urea breath test were 1.0 and 0.98, respectively. The sensitivity and specificity of the rapid urease test were 1.0 and 0.99, respectively. Stool samples were collected from 34 patients from one centre, with a sensitivity and specificity of 1.0 and 0.68, respectively. No defining symptoms ofH pyloriinfection were evident and no peptic ulcer disease was demonstrated.CONCLUSION:H pyloriinfection rates in Canadian children with upper GI symptoms are low, and are lower than those reported for other developed countries. Further studies are required in Canada to determine the prevalence in the general population and specifically in the populations at risk.

scholarly journals Comparing Multiplex PCR and Rapid Urease Test in the Detection ofH. pyloriin Patients on Proton Pump Inhibitors

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Thomas Chen ◽  
Xiangwen Meng ◽  
H. Zhang ◽  
Rebecca W. Tsang ◽  
Tat-Kin Tsang
Keyword(s):  
Multiplex Pcr ◽  
Diagnostic Value ◽  
Control Group ◽  
Rapid Urease Test ◽  
Negative Results ◽  
Multiplex Pcr Assay ◽  
Time Period ◽  
Urease Test ◽  
H Pylori ◽  
The One

Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detectH. pyloriinfection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed.Results. By M-PCR testing, H. pyloriwas detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence ofH. pyloriin 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001).Conclusion. Our PCR is sensitive enough to detect the presence ofH. pyloridespite being on PPI treatment.

scholarly journals Omeprazole, Furazolidone, and Tetracycline: an eradication treatment for resistant H. pylori in Brazilian patients with peptic ulcer disease

2002 ◽  
Vol 57 (5) ◽  
pp. 205-208 ◽  
Author(s):  
Fernando Marcuz Silva ◽  
Jaime Natan Eisig ◽  
Ethel Zimberg Chehter ◽  
Júlio Jovino da Silva ◽  
Antonio Atílio Laudanna
Keyword(s):  
Peptic Ulcer ◽  
Adverse Effects ◽  
Peptic Ulcer Disease ◽  
Breath Test ◽  
Low Cost ◽  
Rapid Urease Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Previously Treated ◽  
H Pylori

OBJECTIVES: To determine the efficacy of a simple, short-term and low-cost eradication treatment for Helicobacter pylori (H. pylori) using omeprazole, tetracycline, and furazolidone in a Brazilian peptic ulcer population, divided into 2 subgroups: untreated and previously treated for the infection. PATIENTS AND METHODS: Patients with peptic ulcer disease diagnosed by endoscopic examination and infected by H. pylori diagnosed by the rapid urease test (RUT) and histological examination, untreated and previously unsuccessfully treated by macrolides and nitroimidazole, were medicated with omeprazole 20 mg daily dose and tetracycline 500 mg and furazolidone 200 mg given 3 times a day for 7 days. Another endoscopy or a breath test was performed 12 weeks after the end of treatment. Patients were considered cured of the infection if a RUT and histologic examination proved negative or a breath test was negative for the bacterium. RESULTS: Sixty-four patients were included in the study. The women were the predominant sex (58%); the mean age was 46 years. Thirty-three percent of the patients were tobacco users, and duodenal ulcer was identified in 80% of patients. For the 59 patients that underwent follow-up examinations, eradication was verified in 44 (75%). The eradication rate for the intention-to-treat group was 69%. The incidence of severe adverse effects was 15%. CONCLUSION: The treatment provides good efficacy for H. pylori eradication in patients who were previously treated without success, but it causes severe adverse effects that prevented adequate use of the medications in 15% of the patients.

scholarly journals Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ashwak M. F. Abu-Taleb ◽  
Randa S. Abdelattef ◽  
Amina A. Abdel-Hady ◽  
Farida H. Omran ◽  
Lobna A. El-korashi ◽  
...  
Keyword(s):  
Peptic Ulcer ◽  
Gastrointestinal Endoscopy ◽  
Gastrointestinal Diseases ◽  
Rapid Urease Test ◽  
University Hospitals ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
Gastric Biopsies ◽  
H Pylori ◽  
The Relationship

H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

scholarly journals Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Rania M. Kishk ◽  
Nashaat M. Soliman ◽  
Maha M. Anani ◽  
Nader Nemr ◽  
Ayman Salem ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Virulence Genes ◽  
Histopathological Examination ◽  
Virulence Gene ◽  
National Study ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

scholarly journals Prevalence of Helicobacter pylori in patients with peptic ulcer disease

2018 ◽  
Vol 5 (4) ◽  
pp. 1315
Author(s):  
Mrutyunjay I. Uppin ◽  
Kapildev K. Hannurkar
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Histopathological Examination ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Urease Test ◽  
H Pylori ◽  
Upper Gastrointestinal

Background: Prevalence of Helicobacter pylori (H. pylori) emerges throughout the world and instigates peptic ulcer disease (PUD). The study was conducted with the aim to determine the prevalence of H. pylori in patients with PUD undergoing upper gastrointestinal endoscopy.Methods: This prospective study was conducted on 150 cases of PUD from August 2009 to February 2011. Endoscopy was done in all cases. Biopsy was done and sent for histopathological examination and rapid urease test for confirmation of presence of H. pylori.Results: Out of 150 patients with mean age of 45.76 years, 109 patients were diagnosed to have been infected with Helicobacter pylori (72.66%). Out of 89 patients with gastric ulcer, 61 patients were infected with Helicobacter pylori (68.53%). Forty two out of 51 patients (82.35%) with duodenal ulcers and 06 of 10 patients (60%) with carcinoma of stomach were positive for H. pylori. The remaining patients were found to be negative for the H. pylori infection.Conclusions: The findings of the study conclude that H. pylori was consistently associated with PUD.

scholarly journals Helicobacter pylori CagA seropositivity in adult Bangladeshi patients with peptic ulcer and erosion

2020 ◽  
Vol 14 (1) ◽  
pp. 36-40
Author(s):  
Fahmida Rahman ◽  
Khandaker Shadia ◽  
Salma Khatun ◽  
Mafruha Mahmud ◽  
Indrajit Kumar Dutta ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Ulcer Disease ◽  
Urease Test ◽  
Urease Production ◽  
Stool Antigen ◽  
H Pylori

Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. Methods: Any case that had peptic ulcer/erosion, plus positive for rapid urease test (RUT) or H. pylori stool antigen (HpSAg) or serum anti-H. pylori IgG/IgA were included in the study and named as H. pylori positive case. H. pylori positive cases were tested for CagA IgG antibody. Anti-H. pylori IgG, IgA and CagA IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and stool antigen by rapid immunochromatographic test (ICT). Urease production in biopsy sample was detected by RUT. Results: Total 86 H. pylori positive patients were included in the study. Out of 86 patients, CagA IgG was positive in 34 (39.5%; 95% CI: 0.30,0.50) cases. CagA seropositivity rate in ulcer and erosion cases were 58.8% (95% CI: 0.36,0.78) and 34.8% (95% CI: 0.25,0.47) respectively. H. pylori stool antigen and IgA antibodies were positive in all (100%) CagA antibody positive ulcer cases while the rates were significantly less among the CagA antibody negative cases (42.8% and 28.6%; p<0.05). However, in CagA antibody positive erosion cases, the rates were not significantly different from CagA antibody negative cases. Conclusion: The study has demonstrated that the CagA positive strain is less prevalent in erosion than ulcer cases. Ibrahim Med. Coll. J. 2020; 14(1): 36-40

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