scholarly journals Comparing Multiplex PCR and Rapid Urease Test in the Detection ofH. pyloriin Patients on Proton Pump Inhibitors

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Thomas Chen ◽  
Xiangwen Meng ◽  
H. Zhang ◽  
Rebecca W. Tsang ◽  
Tat-Kin Tsang
Keyword(s):  
Multiplex Pcr ◽  
Diagnostic Value ◽  
Control Group ◽  
Rapid Urease Test ◽  
Negative Results ◽  
Multiplex Pcr Assay ◽  
Time Period ◽  
Urease Test ◽  
H Pylori ◽  
The One

Background. This study was conducted to assess the diagnostic value of a multiplex PCR assay to detectH. pyloriinfection and to further evaluate the negative results from the CLOtest on patients with and without PPI treatment. Methods. This study is a retrospective cohort that included 457 patients with symptoms of dyspepsia, who underwent upper endoscopy at Evanston and Glenbrook Northshore Hospital from June 2003 to October 2007. A total of 556 samples were reported with some patients having more than one test over the time period. The CLOtest was performed first on the gastric specimen and from that specimen, the DNA was isolated and the one-step multiplex PCR was performed.Results. By M-PCR testing, H. pyloriwas detected in 143 (52.2%) of 274 cases in the control group and 130 (46.1%) of 282 cases in patients on PPI treatment (P=0.1746). The CLOtest detected the presence ofH. pyloriin 4 (1.4%) of 282 cases from the same group receiving PPI treatment and 29 (10.6%) of 274 cases from the group not taking a PPI (P≤0.0001).Conclusion. Our PCR is sensitive enough to detect the presence ofH. pyloridespite being on PPI treatment.

A new understanding of the H. pylori eradication mechanism

2019 ◽  
Author(s):  
Shanshan Su ◽  
Guo-qi Zheng ◽  
Ying-ying Liu ◽  
Yu-fei Liang ◽  
Hui Song ◽  
...  
Keyword(s):  
Gastric Juice ◽  
Intervention Group ◽  
Control Group ◽  
Rapid Urease Test ◽  
Mucus Layer ◽  
Culture Methods ◽  
Detection Rates ◽  
Urease Test ◽  
Gastric Lumen ◽  
H Pylori

Abstract Background: Helicobacter pylori (H. pylori) cannot usually be detected in the gastric juice and it is thought that H. pylori may be implanted under the mucus layer for long term. The mechanisms of action of proton pump inhibitor (PPI), antibiotics, and bismuth for H. pylori eradication are not entirely clear. Our study aimed to determine the role of PPI on the movement of H. pylori across the mucus layer to the gastric lumen and the mechanism of PPI, antibiotics, and bismuth on H. pylori eradication. Methods: Patients with H. pylori infection were intravenous injected with PPI (intervention group, n=31) or without PPI (control group, n=37). The presence of H. pylori in the gastric juice was evaluated by the rapid urease test (RUT), polymerase chain reaction (PCR), and culture methods. Results: The H. pylori positive detection rates were all significantly higher among patients in the intervention group than among patients in the control group by the RUT (P < 0.0001), PCR (P < 0.0001), and culturing (P = 0.0386). Conclusion: H. pylori can penetrate across the mucus layer to the gastric lumen following PPI intervention. The direct antimicrobial activity of PPI might because of diminished numbers of H. pylori due to probiotics in the gastric lumen. Antibiotics and bismuth might play a local sterilization role in the gastric lumen when H. pylori penetrate across the mucus layer.

scholarly journals Relevance of examination and treatment of the most common gastrointestinal disorders in children in Lithuania during the last decade

Medicina ◽  
2007 ◽  
Vol 44 (1) ◽  
pp. 72 ◽  
Author(s):  
Liutauras Labanauskas ◽  
Rūta Kučinskienė ◽  
Vaidotas Urbonas ◽  
Rūta Rokaitė ◽  
Neringa Libikaitė
Keyword(s):  
Duodenal Ulcer ◽  
Atopic Dermatitis ◽  
Abdominal Pain ◽  
Skin Rash ◽  
Endoscopic Examination ◽  
Gastrointestinal Disorders ◽  
Control Group ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

In the last decade, scientific studies in the field of children’s gastroenterology performed in Lithuania explored different problems: pathology of Helicobacter pylori infection and food allergy. Our studies Helirevealed that children with atopic dermatitis had gastrointestinal complaints (abdominal pain, diarrhea, distension and unstable stool, which appeared with the exacerbation of skin rash) more often as compared to nonallergic children of the control group. Abdominal pain in children with atopic dermatitis with local rash was more frequent and lasted longer than in control group children, whereas children with extended rash had stools more frequently. Gastrointestinal disorders in children with atopic dermatitis statistically significantly did not depend on the extent of skin rash and severity of atopic dermatitis. In our scientific research on the importance of H. pylori infection on children’s gastrointestinal system, children with chronic dyspepsia were examined. Endoscopy, rapid urease test, biopsies from antrum and corpus of stomach and their histological examination as well as serologic tests were done. According to the results obtained, we recommend to examine children with chronic dyspepsia in a complex way: not only endoscopic examination, but H. pylori diagnostic tests should be performed as well. Serologic test is not suitable for screening H. pylori infection in children. Considering this, we recommend to use no fewer than two different methods to diagnose this infection. The highest frequency of H. pylori infection was found in children with duodenal ulcer; histological changes in their gastric pylorus and corpus mucosa were greatest. More than half of children with nonulcer dyspepsia were infected with H. pylori. After eradication of H. pylori infection, the prevalence of dyspepsia in children with duodenal ulcer decreased.

scholarly journals Association of TP53 gene codon 72 polymorphism with Helicobacter pylori-positive non-cardia gastric cancer in Vietnam

2019 ◽  
Vol 13 (11) ◽  
pp. 984-991
Author(s):  
Thi Minh Thi Ha ◽  
Thanh Nha Uyen Le ◽  
Viet Nhan Nguyen ◽  
Van Huy Tran
Keyword(s):  
Gastric Cancer ◽  
Control Group ◽  
Rapid Urease Test ◽  
Genotype Distribution ◽  
Codon 72 ◽  
Codon 72 Polymorphism ◽  
Urease Test ◽  
Significant Difference ◽  
Tp53 Codon 72 Polymorphism ◽  
H Pylori

Introduction: This research aimed to determine the association of the combination of H. pylori infection and TP53 codon 72 polymorphism with non-cardia gastric cancer (GC) in Vietnam. Methodology: A total of 164 patients with non-cardia GC and 164 patients with peptic ulcer disease or functional dyspepsia in controls matched by sex and age were enrolled. H. pylori infection was diagnosed by rapid urease test and polymerase chain reaction (PCR). The cagA gene-positivity and vacA sm subtypes were determined by multiplex PCR. Genotypes of TP53 codon 72 polymorphism were determined by PCR-restriction fragment length polymorphism. Results: The prevalence of H. pylori infection in GC and control group were 61.6% and 55.4%, respectively. The rates of cagA-positive strains in the two H. pylori-positive groups were 80.2% and 71.4%, respectively. There was no statistically significant difference in TP53 codon 72 genotype distribution between GC group (frequencies of Arg/Arg, Arg/Pro and Pro/Pro genotypes were 31.1%, 43.3% and 25.6%, respectively) and controls (29.3%, 52.4% and 18.3%, respectively), p = 0.172. The significant difference in genotype distribution was observed in recessive model (Pro/Pro vs Arg/Arg + Arg/Pro) when stratifying by H. pylori infection (OR = 2.02, 95% CI 1.03–3.96, p = 0.041) and by cagA-positivity (OR = 2.33, 95% CI 1.07–5.07, p = 0.032). Conclusions: This study suggests a synergistic interaction between H. pylori infection, especially cagA-positive H. pylori, and Pro/Pro genotype of TP53 codon 72 polymorphism might play a significant role in the pathogenesis of GC in the Vietnamese population.

scholarly journals Evidence of mother-child transmission of Helicobacter pylori infection

2004 ◽  
Vol 41 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Mario Luis Escobar ◽  
Elisabete Kawakami
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Family Members ◽  
Control Group ◽  
Common Source ◽  
Rapid Urease Test ◽  
Source Of Infection ◽  
Group I ◽  
Urease Test ◽  
H Pylori

BACKGROUND: Low socioeconomical status is a major risk factor for natural acquisition of Helicobacter pylori (H. pylori) infection in developing countries. Its transmission route is unknown but studies suggest person-to-person transmission. AIM: To evaluate seropositivity of anti-H. pylori antibodies in family members of infected symptomatic index patients as compared to family members of symptomatic uninfected index patients. PATIENTS AND METHODS: One hundred and twelve family members of 38 patients who underwent endoscopy to exclude peptic disease were studied. Patients were deemed H. pylori infected or not infected when rapid urease test and histology were both positive or both negative. The family members underwent ELISA serology using the Cobas Core II Kit (Roche) and were classified into three groups: I - 29 family members of 10 H. pylori (+) duodenal ulcer index patients; II - 57 family members of 17 H. pylori (+) index patients without duodenal ulcer; III - 26 family members of 11 H. pylori (-) index patients. RESULTS: Seropositivity of group I and II (infected patients) was higher than the control group, 83% vs 38%, specially in mothers, 81% vs 18%, and in siblings 76% vs 20%. Differences between fathers' seropositivity was not statistically significant in the three groups: 100% vs 86% vs 70%. Seropositivity of all family members (mother, father and siblings) between infected group (I vs II) was similar. CONCLUSION: Prevalence of H. pylori infection was higher in family members of infected patients, but was similar among family members of infected patients with and without duodenal ulcer. H. pylori infection is more frequent in mothers and siblings of infected index children. A common source of infection cannot be excluded, but facts suggest that person-to-person transmission occurs, specially from mother to child.

scholarly journals Identification of Helicobacter pylori DNA in Iranian patients with gallstones

2004 ◽  
Vol 132 (6) ◽  
pp. 1185-1189 ◽  
Author(s):  
SH. FARSHAD ◽  
A. ALBORZI ◽  
S. A. MALEK HOSSEINI ◽  
B. OBOODI ◽  
M. RASOULI ◽  
...  
Keyword(s):  
Gallstone Formation ◽  
Control Group ◽  
Rapid Urease Test ◽  
Biliary Disease ◽  
Clinical Role ◽  
Test Culture ◽  
Urease Test ◽  
H Pylori ◽  
Bile Samples ◽  
Iranian Patients

In order to identify Helicobacter in gallstones of Iranian patients with biliary disease, gallstone and bile samples from 33 patients were subjected to rapid urease test, culture and Multiplex PCR using primers based on 16s rRNA and isocitrate dehydrogenase genes for the identification of Helicobacter genus and H. pylori respectively. This PCR was also done on bile samples from 40 autopsied gallbladders with normal pathology (control group). In 18·1% of stone and 12·1% of bile samples, H. pylori DNA was detected using PCR. Rapid urease and culture tests were negative for all samples. The PCR was negative in the control group. In conclusion, H. pylori DNA was detected in stone samples of Iranian patients with gallstones but we are not sure of their viability. To clarify the clinical role of Helicobacter in gallbladder diseases, studies using accurate tests on larger patient and control groups are needed to ascertain whether this microorganism is an innocent bystander or active participant in gallstone formation.

scholarly journals Comparison of Endoscopy-Based and Serum-Based Methods for the Diagnosis ofHelicobacter pylori

2003 ◽  
Vol 17 (2) ◽  
pp. 101-106 ◽  
Author(s):  
Elvira Garza-González ◽  
Francisco J Bosques-Padilla ◽  
Rolando Tijerina-Menchaca ◽  
Juan P Flores-Gutiérrez ◽  
Héctor J Maldonado-Garza ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Diagnostic Value ◽  
The Body ◽  
Rapid Urease Test ◽  
Igg Antibodies ◽  
Ulcer Disease ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori ◽  
Age Range

Available commercial tests for the diagnosis ofHelicobacter pyloriinfection are based on different types of antigen preparations and hence the diagnostic utility differs substantially.OBJECTIVE: To assess the diagnostic value of the determination of Immunoglobulin (Ig) A and IgG antibodies toH pyloriwhole cell (WC) and IgG antibodies to cytotoxin associated gene A (CagA) using an in-house ELISA in relation to the results obtained with different invasive methods.METHODS: The study population consisted of 251 Mexican adults, mean age 53 years, age range 15 to 92 years and female to male ratio of 1.5. Peptic ulcer disease was present in 10.8% of these patients, 5.2% had gastric cancer, 11.2% had esophagitis and 72.9% had nonulcer dyspepsia. Biopsy specimens from the body and the antrum of the stomach were obtained for culture, histology and rapid urease test. ELISAs to detect IgA and IgG WC andCagAantibodies were performed using serum.RESULTS:H pyloristatus was established by the results of the invasive tests. Eighty (31.9%) patients positive to the three tests and 38 (15.1%) negative to all the tests were identified. Based on this result, the sensitivity and specificity of the serology assays were 97.5% and 78.9% for the IgG WC and 70% and 73.7% for the IgA WC, respectively. However, ifH pyloristatus was defined by the positive result of at least one or two invasive diagnostic tests, the sensitivity for the IgG WC decreased to 87.3% and 66.7% respectively, but the specificity was essentially the same. Similar results were obtained for the sensitivity and specificity of IgA using the same criteria. A lowCagAprevalence was observed (39%).CONCLUSIONS: Testing for serological IgG antibodies toH pyloriWC was the best to assess whether infection byH pyloriwas present. Neither the IgA WC nor the IgGCagAELISAs add significant value in the diagnosis ofH pylori.

Rapid urease test for H. pylori diagnosis: pros and cons

2010 ◽  
Vol 5 (3) ◽  
pp. 257-258 ◽  
Author(s):  
Angelo Zullo ◽  
Cesare Hassan ◽  
Silvia Trapani ◽  
Gianfranco Tammaro
Keyword(s):  
Rapid Urease Test ◽  
Urease Test ◽  
Pros And Cons ◽  
H Pylori

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

scholarly journals Incidence of Gastric Metaplasia and Helicobacter pylori Infection in Duodenal Bulb – With Specific Reference to Patients With Duodenal Ulcers

1999 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Minoru Kawaguchi ◽  
Toshihiko Saito
Keyword(s):  
Helicobacter Pylori ◽  
Duodenal Bulb ◽  
Mucosal Injury ◽  
Transitional Zone ◽  
Specific Reference ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Gastric Metaplasia ◽  
Urease Test ◽  
H Pylori

We determined the incidence of gastric metaplasia in the duodenal bulb of duodenal ulcer patients and the Helicobacter pylori (H. pylori) infection rate at sites with gastric metaplasia. Biopsy of the duodenal bulb showed the presence of gastric metaplasia in 61 of 86 patients (71%) overall and in 18 of 47 patients (38.3%) who had gastrectomy at an early gastric cancer. The histological diagnosis of H. pylori infection showed good agreement (83.3%) with the result of the rapid urease test, indicating that H. pylori occurs in regions with gastric metaplasia. This finding suggests that H. pylori infects gastric metaplasia in the duodenal bulb, causing mucosal injury, which is then transformed into duodenal ulcers. The exact mechanism by which gastric metaplasia is caused is unknown, but it is believed to occur in the transitional zone in the duodenal mucosa.

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