scholarly journals Clinical Validation of an Office-Based14C-UBT (Heliprobe) forH. pyloriDiagnosis in Iranian Dyspeptic Patients

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Omid Sanaei ◽  
Farahnaz Joukar
Keyword(s):  
Predictive Value ◽  
Gold Standard ◽  
Urea Breath Test ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
Clinical Validation ◽  
Igg Antibodies ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background. We encountered repeatedly, in our clinical practice, discordant results between UBT and histopathology aboutH. pyloriinfection.Goal. To study the diagnostic accuracy of Heliprobe14C-urea breath test (14C-UBT) for detection ofH. pyloriinfection in an Iranian population.Study. We enrolled 125 dyspeptic patients in our study. All of them underwent gastroscopy, and four gastric biopsies (three from the antrum and one from the corpus) were obtained. One of the antral biopsies was utilized for a rapid urease test (RUT), and three others were evaluated under microscopic examination. Sera from all patients were investigated for the presence ofH. pyloriIgG antibodies. The14C-UBT was performed on all subjects using Heliprobe kit, and results were analyzed against the following gold standard (GS):H. pyloriinfection considered positive when any two of three diagnostic methods (histopathology, RUT, serology) are positive.Results. According to data analysis, the Heliprobe14C-UBT had 94% sensitivity, 100% specificity, 93% negative predictive value (NPV), 100% positive predictive value (PPV), and 97% accuracy, compared with GS.Conclusion. The Heliprobe14C-UBT is an easy-to-perform, rapid-response, and accurate test forH. pyloridiagnosis, suitable for office use.

scholarly journals Helicobacter pylori culture as a key tool for diagnosis in Colombia

2019 ◽  
Vol 13 (08) ◽  
pp. 720-726
Author(s):  
Diana F Rojas-Rengifo ◽  
Belen Mendoza ◽  
Carlos Jaramillo ◽  
Paula A Rodríguez-Urrego ◽  
José F Vera-Chamorro ◽  
...  
Keyword(s):  
Predictive Value ◽  
Optimal Growth ◽  
Rapid Urease Test ◽  
Culture Methods ◽  
Essential Information ◽  
Urease Enzyme ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Sensitivity Specificity

Introduction: The presence of H. pylori in the stomach is associated with gastric pathologies. However, its diagnosis through culture methods is challenging because of its complex nutritional requirements and microaerophilic conditions for optimal growth. The preferred method for rapid diagnosis of H. pylori is the Rapid Urease Test (RUT) from human biopsies, which relies on the high activity of the urease enzyme present in H. pylori. However, RUT cannot say much more information about H. pylori. This makes evident the need for bacterial culture to know essential information such as the strain type, the kind of infection present and the bacteria’s antibiotic susceptibility. Methodology: Gastric biopsies from 347 patients were used for H. pylori isolation. We correlated the culture results with the RUT and histological grading used at Hospital Universitario Fundación SantaFe de Bogotá (HU-FSFB), Colombia. The concordance between techniques was determined by the Cohen’s Kappa coefficient (K). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were also calculated. Results: The culture standardization was successful, and it could be applied for diagnosis in the clinical practice. H. pylori was positive by culture in 88 (26.34%) patients. The concordance of RUT and culture was strong (K= 0.805), and between histology and culture was moderate (K= 0.763) as well as for the gold standard defined and culture (K= 0.80). Conclusions: We present evidence that RUT and histological methods will be better interpreted for diagnosis of H. pylori if combined with bacterial isolation in cholesterol enriched culture.

scholarly journals Reliability of Diagnostic Tests forHelicobacter pyloriInfection

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
S. Redéen ◽  
F. Petersson ◽  
E. Törnkrantz ◽  
H. Levander ◽  
E. Mårdh ◽  
...  
Keyword(s):  
Histological Examination ◽  
Gold Standard ◽  
Diagnostic Tests ◽  
Breath Test ◽  
Eradication Therapy ◽  
Urea Breath Test ◽  
Rapid Urease Test ◽  
First Choice ◽  
Urease Test ◽  
H Pylori

Introduction.Helicobacter pylori (H. pylori)infection is very common worldwide. A reliable diagnosis is crucial for patients withH. pylori-related diseases. At followup, it is important to confirm that eradication therapy has been successful. There is no established gold standard for the diagnosis ofH. pyloriinfection.Material and Methods. A sample of 304 volunteers from the general population was screened forH. pyloriinfection with serology,13C-urea breath test (UBT), rapid urease test (RUT) on fresh biopsy, culture from biopsy, and histological examination. Culture was used as gold standard.Results. The sensitivity was 0.99 for serology, 0.90 for UBT, 0.90 for RUT, and 0.90 for histological examination. Corresponding specificities were 0.82, 0.99, 0.98, and 0.97, respectively. The accuracy was 0.86 for serology, 0.96 for UBT, 0.95 for RUT, 0.93 for culture, and 0.95 for histology. There was a strong correlation between the results of UBT and the histological scores ofH. pyloricolonisation as well as between the results of UBT and the scores of RUT.Conclusion. There were only minor differences in accuracy between the three invasive tests forH. pyloriinfection in this population. RUT may be recommended as the first choice since a result is obtained within hours. The accuracy of UBT was comparable to the invasive tests, and it is recommended for situations when endoscopy is not needed.

scholarly journals Prospective study to evaluate the number and the location of biopsies in rapid urease test for diagnosis of Helicobacter Pylori

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Antoine Abou Rached ◽  
Jowana Saba ◽  
Cesar Yaghi ◽  
Joyce Sanyour ◽  
Ahmad El Hajjar ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Rapid Test ◽  
Rapid Urease Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Antral Biopsy

Helicobacter pylori (H. pylori) can cause a wide variety of illnesses such as peptic ulcer disease, gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. The diagnosis and eradication of H. pylori are crucial. The diagnosis of H. pylori is usually based on the rapid urease test (RUT) and gastric antral biopsy for histology. The aim of this study is to evaluate the numbers of needed biopsies and their location (antrum/fundus) to obtain optimal result for the diagnosis of H. pylori. Three hundred fifty consecutive patients were recruited, 210 fulfill the inclusion criteria and had nine gastric biopsies for the detection of H. pylori infection: two antral for the first RUT (RUT1), one antral and one fundic for the second (RUT2), one antral for the third (RUT3) and two antral with two fundic for histology (HES, Giemsa, PAS). The reading of the 3 types of RUT was performed at 1 hour, 3 hours and 24 hours and biopsies were read by two experienced pathologists not informed about the result of RUT. Results of RUT were considered positive if H. pylori was found on histology of at least one biopsy. The RUT1 at 1h, 3h and 24h has a sensitivity of 72%, 82% and 89% and a specificity of 100%, 99% and 87% respectively. The positive predictive value (PPV) was 100%, 99% and 85% respectively and the negative predictive value (NPV) of 81%, 87% and 90%. The RUT2 at 1h, 3h and 24h, respectively, had a sensitivity of 86%, 87% and 91% and a specificity of 99%, 97% and 90%. The PPV was 99%, 96% and 88% and NPV of 89%, 90%, 94%. The RUT3 at 1h, 3h and 24h, respectively, had a sensitivity of 70%, 74% and 84% and a specificity of 99%, 99% and 94%. The PPV was 99%, 99% and 92% and NPV of 79%, 81% and 87%. The best sensitivity and specificity were obtained for RUT1 read at 3h, for RUT2 read 1h and 3h, and the RUT3 read at 24h.This study demonstrates that the best sensitivity and specificity of rapid test for urease is obtained when fundic plus antral biopsy specimens are used with a reading time at 3 hours.

scholarly journals Comparison of home made and commercial rapid urease tests for detection of helicobacter pylori in patients with gastroduodenitis and peptic ulcer

2015 ◽  
Vol 1 (2) ◽  
pp. 11-14
Author(s):  
Yuba Raj Sharma ◽  
Amrita Wagley ◽  
Sunil Singh
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Predictive Value ◽  
Gold Standard ◽  
Rapid Urease Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
The Common ◽  
H Pylori ◽  
Sensitivity Specificity

Introductions: Helicobacter pylori is one of the common and medically prominent infections worldwide and an established etiological factor for peptic ulcer disease. This study was conducted to compare the results of two types of Rapid Urease Tests (RUT) for H. pylori infection.Methods: This study was conducted in patients with gastro duodenal diseases visiting Kantipur Hospital from June to August 2010. Antral biopsies were collected from sixty patients visiting endoscopy unit. The diagnosis was of H. pylori infection carried out using two types of rapid urease tests (commercial and homemade) as well as Histopathology.Results: H. pylori infection was detected in 34 (56.67%) of 60 by histological test, 24 (40%) by homemade kit method and 28 (46.67%) by commercial RUT method. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for RUT (commercial kit) considering histology as gold standard were 76.74%, 92.31%, 92.85% and 75% respectively. The sensitivity, specificity, PPV and NPV for RUT (homemade kit) were 58.82%, 84.62%, 83.3% and 61.11% respectively.Conclusions: Homemade rapid urease test was sensitive and specific for detection of H. pylori infection than commercial test.

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

scholarly journals Antibiotic Resistance and Genotypes of Helicobacter pylori Strains in Patients with Gastroduodenal Disease in Southeast Poland

2019 ◽  
Vol 8 (7) ◽  
pp. 1071 ◽  
Author(s):  
Izabela Korona-Glowniak ◽  
Halina Cichoz-Lach ◽  
Radoslaw Siwiec ◽  
Sylwia Andrzejczuk ◽  
Andrzej Glowniak ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Helicobacter Pylori ◽  
Test Method ◽  
Rapid Urease Test ◽  
Microbial Culture ◽  
Virulence Markers ◽  
Gastroduodenal Disease ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

The aim of this study was to investigate genetic diversity of Helicobacter pylori virulence markers to predict clinical outcome as well as to determine an antibiotic susceptibility of H. pylori strains in Poland. Gastric biopsies from 132 patients with gastrointestinal disorders were tested for presence of H. pylori with the use of rapid urease test, microbial culture, and polymerase chain reaction (PCR) detection. The genetic diversity of 62 H. pylori positive samples was evaluated by detection of cagA and PCR-typing of vacA and iceA virulence-associated genes. Most common H. pylori genotypes were cagA(+)vacAs1m2 (27.4%) and cagA(−)vacAs2m2 (24.2%). In logistic regression analysis, we recognized the subsequent significant associations: gastritis with ureC, i.e., H. pylori infection (p = 0.006), BMI index (p = 0.032); and negatively with iceA1 (p = 0.049) and peptic ulcer with cagA (p = 0.018). Thirty-five H. pylori strains were cultured and tested by E-test method showing that 49% of strains were resistant to at least one of the tested antibiotics. This is the first study that reports the high incidence and diversity of allelic combination of virulence genes in gastroduodenitis patients in Poland. Genotyping of H. pylori strains confirmed the involvement of cagA gene and vacAs1m1 genotype in development and severity of gastric disorder.

Good diagnostic accuracy of a chemiluminescent immunoassay in stool samples for diagnosis of Helicobacter pylori infection in patients with dyspepsia

2016 ◽  
Vol 64 (2) ◽  
pp. 388-391 ◽  
Author(s):  
María José Ramírez-Lázaro ◽  
Josep Lite ◽  
Sergio Lario ◽  
Pepa Pérez-Jové ◽  
Antònia Montserrat ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gold Standard ◽  
Rapid Urease Test ◽  
Diagnostic Reliability ◽  
Predictive Values ◽  
Urease Test ◽  
Stool Samples ◽  
H Pylori ◽  
Good Diagnostic Accuracy ◽  
Sensitivity Specificity

Laboratory-based chemiluminescence immunoassays (CLIA) are widely used in clinical laboratories. Some years ago, a CLIA test was developed for the detection of Helicobacter pylori in stool samples, known as LIAISON H. pylori SA, but little information on its use has been reported. To evaluate the accuracy of the LIAISON H. pylori SA assay for diagnosing H. pylori infection prior to eradication treatment. Diagnostic reliability was evaluated in 252 untreated consecutive patients with dyspepsia. The gold standard for diagnosing H. pylori infection was defined as the concordance of the rapid urease test (RUT), histopathology and urea breath test (UBT). The CLIA assay was performed according to the manufacturer's instructions. Sensitivity, specificity, positive and negative predictive values, and 95% CIs were calculated. According to the gold standard selected, 121 patients were positive for H. pylori infection and 131 negative. LIAISON H. pylori SA had a sensitivity of 90.1% and a specificity of 92.4%, with positive and negative predictive values of 91.6% and 90.1%, respectively. The accuracy of the LIAISON H. pylori SA chemiluminescent diagnostic assay seems comparable to that of ELISA or the best-performing LFIAs. Its sensitivity and specificity, however, seem slightly lower than those of histology, RUT or UBT. The advantages of the assay are that it is cheap, automated, and minimally labor-intensive.

scholarly journals Low Prevalence ofHelicobacter PyloriInfection in Canadian Children: A Cross-Sectional Analysis

2008 ◽  
Vol 22 (5) ◽  
pp. 485-489 ◽  
Author(s):  
Idit Segal ◽  
Anthony Otley ◽  
Robert Issenman ◽  
David Armstrong ◽  
Victor Espinosa ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Breath Test ◽  
Tertiary Care ◽  
Urea Breath Test ◽  
Rapid Urease Test ◽  
Infection Rates ◽  
Upper Gi ◽  
Gi Symptoms ◽  
Urease Test ◽  
H Pylori

BACKGROUND: The incidence and prevalence rates of childhoodHelicobacter pyloriinfection vary greatly by nation, with infection rates of 8.9% to 72.8% reported in developed and developing countries, respectively. To date, few studies have assessed the prevalence ofH pyloriin Canadian children, with studies limited to Aboriginal communities and single tertiary care centres from Ontario and Quebec.OBJECTIVES: To determine the prevalence ofH pyloriin consecutive children referred to three Canadian tertiary care academic centres for upper gastrointestinal (GI) endoscopy due to upper GI symptoms, and to determine the sensitivity and specificity of the carbon-13-labelled urea breath test, the rapid urease test and theH pyloristool monoclonal antigen test.RESULTS: Two hundred four patients were recruited. The prevalence ofH pyloriwas 7.1%. Of theH pylori-positive patients, 41.7% were male, with a mean age of 10.3 years. Ethnic minorities accounted for 42% of theH pylori-positive patients. Consistent with previous observations, the sensitivity and specificity of the carbon-13-labelled urea breath test were 1.0 and 0.98, respectively. The sensitivity and specificity of the rapid urease test were 1.0 and 0.99, respectively. Stool samples were collected from 34 patients from one centre, with a sensitivity and specificity of 1.0 and 0.68, respectively. No defining symptoms ofH pyloriinfection were evident and no peptic ulcer disease was demonstrated.CONCLUSION:H pyloriinfection rates in Canadian children with upper GI symptoms are low, and are lower than those reported for other developed countries. Further studies are required in Canada to determine the prevalence in the general population and specifically in the populations at risk.

scholarly journals Upper socioeconomic status is associated with lower Helicobacter pylori infection rate among patients undergoing gastroscopy

2020 ◽  
Vol 14 (03) ◽  
pp. 298-303 ◽  
Author(s):  
Tan Attila ◽  
Mujdat Zeybel ◽  
Yesim Esen Yigit ◽  
Bulent Baran ◽  
Emel Ahishali ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Predictive Value ◽  
Young Patients ◽  
University Hospital ◽  
Urban Region ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Urease Test ◽  
Gastric Biopsies ◽  
Period Data

Introduction: Socioeconomic factors play an important role in the prevalence of Helicobacter pylori (HP) infection. The aim of this study is to investigate HP prevalence among symptomatic patients in the upper socioeconomic segment of the population undergoing gastroscopy in an endemic urban region. Methodology: Over a 12-month period, data were collected from the first consecutive 1000 patients (500 from university hospital, 500 from community hospital) who had gastroscopy and HP evaluation. Results: Overall, 211/1000 patients (21.1 %) were found to have HP in gastric biopsies. The specificity, sensitivity, positive predictive value, negative predictive value and diagnostic accuracy of rapid urease test were 87.5%, 99.7%, 99%, 96.5%, and 96.9% respectively. Atrophic gastritis, gastric and duodenal ulcers were significantly more common in HP positive patients. Age based distribution of HP prevalence: > 6 decades (15.5%), 3rd-5th decades (26.1%), < 3rd decades (10.4%). Conclusion: In an HP endemic country, the prevalence of HP infection among symptomatic patients belonging to the upper socioeconomic segment of the population appears to be markedly lower. The lowest prevalence in young patients is expected to result in future decrease in HP prevalence.

scholarly journals Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ashwak M. F. Abu-Taleb ◽  
Randa S. Abdelattef ◽  
Amina A. Abdel-Hady ◽  
Farida H. Omran ◽  
Lobna A. El-korashi ◽  
...  
Keyword(s):  
Peptic Ulcer ◽  
Gastrointestinal Endoscopy ◽  
Gastrointestinal Diseases ◽  
Rapid Urease Test ◽  
University Hospitals ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
Gastric Biopsies ◽  
H Pylori ◽  
The Relationship

H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

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