scholarly journals Alkaline Phosphatase and Disaccharide Activity in Barrett's Columnar Lined Esophagus

1988 ◽  
Vol 2 (1) ◽  
pp. 15-17
Author(s):  
S.N. Sullivan ◽  
R.J. Darwish ◽  
M. Troster
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Biochemical Markers ◽  
Squamous Epithelium ◽  
Disaccharidase Activity ◽  
Columnar Lined Esophagus ◽  
The Mean ◽  
Small Intestinal ◽  
Standard Deviations

Esophageal mucosal biopsies of 15 patients with normal squamous epithelium, 11 with inflamed squamous epithelium and 23 with Barrete's columnar lined esophagus were assayed for alkaline phosphatase and disaccharidase activity. Seven of the patients with Barrett's esophagus had alkaline phosphatase activity greater than three standard deviations above the mean of the 15 patients with normal squamous epithelium. Five of these seven also had disaccharidase activity three standard deviations or greater above normal. One patient with esophagitis, but without columnar epithelium, had a small increase in alkaline phosphatase and disaccharidase activity. The production of biochemical markers of small intestinal structure may he another indicator of the pluripotential nature of Barrett's epithelium.

Small-Intestinal Changes Induced by an Elemental Diet (Vivonex) in Normal Rats

1978 ◽  
Vol 55 (5) ◽  
pp. 509-511 ◽  
Author(s):  
L. M. Nelson ◽  
H. A. Carmichael ◽  
R. I. Russell ◽  
F. D. Lee
Keyword(s):  
Alkaline Phosphatase ◽  
Protein Content ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Elemental Diet ◽  
Normal Diet ◽  
Villus Height ◽  
Disaccharidase Activity ◽  
Control Value ◽  
Small Intestinal

1. Rats were fed with the elemental diet Vivonex for 1 or 3 months and their jejunal histology was compared with that of an equal number of rats fed on a normal diet. 2. After 1 month of Vivonex feeding a significant reduction in the ratio of crypt height: villus height (CH: VH) was found in the Vivonex-fed rats (n = 4) compared with the control rats (n = 4) (P < 0.05). 3. After 3 months the CH:VH ratio was also reduced in the Vivonex-fed rats (n = 18) compared with control rats (n = 18) (P < 0.002). Villus height was significantly increased (P < 0.002) and crypt height decreased (P < 0.05). 4. Jejunal protein content, alkaline phosphatase and disaccharidase activity were also determined in 12 control and 12 Vivonex-fed rats from the 3 months study. 5. Alkaline phosphatase activity was increased from a control value of 201 ± 8 to 243 ± 15 munits/cm in the Vivonex-fed rats (n = 12) (P < 0.05) but no significant changes in lactase, sucrase or maltase activities were found. 6. The observed decrease in the CH:VH ratio suggested an improved survival of the mature enterocyte population during elemental diet feeding.

Decreased Abundance of Alkaline Phosphatase and Calbindin-D9K mRNAs in the Intestine of the Spontaneously Hypertensive Rat

1992 ◽  
Vol 38 (12) ◽  
pp. 2526-2531 ◽  
Author(s):  
D T Leetun ◽  
M E Bruns ◽  
D E Bruns
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Specific Activity ◽  
Spontaneously Hypertensive Rat ◽  
Similar Extent ◽  
Spontaneously Hypertensive ◽  
Wky Rats ◽  
Calbindin D9k ◽  
The Mean

Abstract Alkaline phosphatase activity and calbindin-D9K immunoreactivity are decreased in the intestines of spontaneously hypertensive rats (SHRs). To investigate the potential role of altered gene expression in these decreases, we measured, by Northern blot analyses, the abundances of alkaline phosphatase and calbindin-D9K mRNAs in the proximal regions of the small intestines of 14-week-old SHR and control Wistar-Kyoto (WKY) rats. Alternate 4-cm segments of intestine were used for measurements of the proteins (0-4 cm, 8-12 cm, and 16-20 cm from pylorus, segments A1, B1, and C1, respectively) and mRNAs (4-8 cm, 12-16 cm, and 20-24 cm, segments A2, B2, and C2). Calbindin-D9K (immunoassay) was decreased in SHR vs WKY rats by 27%, 64%, and 67% in segments A1, B1, and C1, respectively (P &lt; 0.01); its mRNA was decreased to a similar extent (69%, 82%, and 80%, respectively; P &lt; 0.002 by analysis of variance). Alkaline phosphatase activity was decreased in SHRs by 58%, 54%, and 51% in segments A1, B1, and C1, respectively (P &lt; 0.01); the abundance of its 3.0-kb mRNA was decreased to a similar extent: 57%, 80%, and 69% in segments A2, B2, and C2, respectively (P &lt; 0.02). The mean decreases of the 2.7-kb mRNA of alkaline phosphatase were statistically significant (P &lt; 0.02) but smaller (38%, 40%, and 35%). The mean abundance of vitamin D receptor mRNA in the same animals was decreased slightly in SHR vs WKY rats (3%, 36%, and 20% in segments A2, B2, and C2, respectively), but the difference in the values was not statistically significant. Decreases in alkaline phosphatase activity and calbindin-D9K immunoreactivity may reflect decreased mRNA abundance and not decreased enzyme-specific activity or increased protein degradation.

Plasma B-6 Vitamer Changes Following a 50-km Ultramarathon

2000 ◽  
Vol 10 (3) ◽  
pp. 302-314 ◽  
Author(s):  
Scott W. Leonard ◽  
James E. Leklem
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Plasma Glucose ◽  
Plasma Concentrations ◽  
Blood Samples ◽  
Liquid Intake ◽  
Physical Conditions ◽  
The Mean

The purpose of this study was to measure plasma B-6 vitamers, and other factors which may affect the plasma concentrations of these vitamers under extreme physical conditions. Blood samples were drawn from 8 men and 3 women (43.7 ± 8.6 years) 30 min prior to the start of a 50-km ultramarathon race (pre), and at 5 (PST) and 60 (PST60) min post race. HPLC was used to measure plasma pyridoxal 5’-phosphate (PLP), pyridoxal (PL), pyridoxine (PN), and 4-pyridoxic acid (4-PA). Plasma glucose, albumin, lactate, and alkaline phosphatase activity, as well as hematocrit, and hemoglobin levels were measured. Food and liquid intake was assessed during the run. There was a significant (p < .001) decrease in the plasma PLP concentration between pre and PST, with a mean decrease of 12.9 ± 8.8 nmol/L (31% decrease). At PST60, there was a further decrease in plasma PLP concentration bringing the total decrease to 17.9 nmol/L (44%). The plasma TB6 concentration also decreased after the run, but the mean decrease was only 13.5 nmol/L (pre to PST60). PL increased 25% after the run, and did not change further at PST60. The mean plasma 4-PA concentration increased 21% post run and decreased to just below the pre-run value 1 hr post race. The plasma PLP decrease measured in the current study is not consistent with what has previously been reported during shorter length endurance studies.

scholarly journals Correlations between Serum Interleukins-2,-4 Levels and Some Biochemical Parameters in Iraqi Patients with Osteoporosis

2017 ◽  
Vol 59 (3) ◽  
pp. 275-279
Author(s):  
Hind Sh. Ahmed
Keyword(s):  
Alkaline Phosphatase ◽  
Lipid Profile ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Biochemical Markers ◽  
Fasting Glucose ◽  
Serum Alkaline Phosphatase ◽  
Interleukin 2 ◽  
Interleukin 4 ◽  
Serum Alkaline Phosphatase Activity

Background: Osteoporosis is a frequent disease that is manifested by reduced in mineral density and raised in fracture risk. Recent studies have indicated that osteoporosis is caused by composite connections among local and systemic regulators of bone cell function.Objective: The purpose of this study was to investigate the relationship between interleukin-2, interleukin-4, and some biochemical markers in Iraqi patients with osteoporosis.Patients and Methods: Forty five osteoporotic patients were incorporated in this study (30 women and 15 men). Serum fasting glucose, lipid profile, alkaline phosphatase activity, calcium, magnesium, interleukin-2, and interleukin-4 were measured in osteoporotic patients and compared them with the control group.Results: There was a significant increase in serum fasting glucose, lipid profile except high density lipoprotein cholesterol, and serum alkaline phosphatase activity in osteoporotic patients as compared to the control, (P=0.001). Also, there was a significant increase in interleukin-2 and interleukin-4 in osteoporotic patients as compared to the control, (P= 0.01). While there was a decrease in serum calcium and magnesium levels in osteoporotic patients as compared to the control. Also, there was a significant positive correlation between serum alkaline phosphatase activity with interleukin-2 and interleukin-4 levels in osteoporotic patients, (P= 0.01).Conclusion: Elevated levels of interleukin-2 and interleukin-4 along with some biochemical markers like; serum alkaline phosphatase in osteoporotic patients might include significant functions in the pathogenesis of osteoporosis.

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