Lipopolysaccharide Exhibits Synergistic Enhancement of Butyrate-Induced and Retinoic Acid-Mediated Alkaline Phosphatase Activity on Small Intestinal Epithelial Cell Line, IEC-6

Digestion ◽  
1998 ◽  
Vol 59 (6) ◽  
pp. 683-688 ◽  
Author(s):  
Kouhei Fukushima ◽  
Iwao Sasaki ◽  
Ken-ichi Takahashi ◽  
Hiroo Naito ◽  
Hitoshi Ogawa ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Retinoic Acid ◽  
Epithelial Cell ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Intestinal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Synergistic Enhancement ◽  
Small Intestinal

scholarly journals Hydrocortisone induces changes in gene expression and differentiation in immature human enterocytes

2011 ◽  
Vol 300 (3) ◽  
pp. G425-G432 ◽  
Author(s):  
Lei Lu ◽  
Tiantian Li ◽  
Graham Williams ◽  
Elizabeth Petit ◽  
Mark Borowsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Molecular Mechanisms ◽  
Cell Maturation ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Tight Junction Formation ◽  
Expression Of Genes ◽  
Small Intestinal

It is known that functional maturation of the small intestine occurring during the weaning period is facilitated by glucocorticoids (such as hydrocortisone, HC), including an increased expression of digestive hydrolases. However, the molecular mechanisms are not well understood, particularly in the human gut. Here we report a microarray analysis of HC-induced changes in gene expression in H4 cells (a well-characterized human fetal small intestinal epithelial cell line). This study identified a large number of HC-regulated genes, some involved in metabolism, cell cycle regulation, cell-cell or cell-extracellular matrix communication. HC also regulates the expression of genes important for cell maturation such as development of cell polarity, tight junction formation, and interactions with extracellular matrices. Using human small intestinal xenografts, we also show that HC can regulate the expression of genes important for intestinal epithelial cell maturation. Our dataset may serve as a useful resource for understanding and dissecting the molecular mechanisms of intestinal epithelial cell maturation.

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