Relationship between plasma corticosterone and adrenal epinephrine after diazepam treatment in rats

1982 ◽  
Vol 60 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Guy Chabot ◽  
Yves Brissette ◽  
André L. Gascon
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Plasma Corticosterone ◽  
The Other ◽  
Dose Effect ◽  
Epinephrine Administration ◽  
Linear Dose ◽  
Increased Activity

Following recent observations that diazepam treatment increases adrenal epinephrine in rats, we were interested in studying the possible mechanisms of this action of diazepam on rat adrenal glands. All diazepam treatments studied (1–25 mg∙kg−1∙day−1 for 10 days) led to an increase in adrenal epinephrine following a linear dose–effect relationship. Since epinephrine synthesis is under neuronal and humoral controls, we investigated their respective importance in the effect of diazepam on the adrenal gland. The denervation of the adrenal gland did not prevent the increase in adrenal epinephrine by diazepam treatment. On the other hand, diazepam treatment was shown to cause an increase in plasma corticosterone in parallel with an increase in adrenal epinephrine. Administration of dexamethasone (a synthetic corticoid) and hypophysectomy prevented the increase in adrenal epinephrine and plasma corticosterone resulting from diazepam treatment. We thus conclude that the increase in adrenal epinephrine seen after diazepam treatment is parallel to the increase in plasma corticosterone. Moreover, since the action of diazepam on adrenal epinephrine is prevented by dexamethasone or hypophysectomy, we hypothesize that diazepam is acting on the adrenal cortex via the release of adrenocorticotropic hormone (ACTH). ACTH and corticosterone would be responsible for the increased activity of epinephrine-synthesizing enzymes in adrenal medulla.

METABOLISM IN VITRO OF TRITIATED ADRENOCORTICOTROPIC HORMONE

1965 ◽  
Vol 43 (9) ◽  
pp. 1489-1498 ◽  
Author(s):  
Edward E. Nishizawa ◽  
R. B. Billiar ◽  
J. Karr ◽  
Kristen B. Eik-Nes
Keyword(s):  
Biological Activity ◽  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Enzymatic Degradation ◽  
Adrenocorticotropic Hormone ◽  
Specific Radioactivity ◽  
Adrenocorticotrophic Hormone ◽  
Serine Residue

Adrenocorticotrophic hormone (ACTH) labeled with tritium has been prepared with a specific radioactivity of approximately 4000 d.p.m./μg. The tritiated trophin showed biological activity and appeared to be bound to the cells of the adrenal gland. Data indicating that the adrenal cortex can inactivate ACTH were obtained; whether such inactivation is due to binding or enzymatic degradation has not been illustrated. Attempts to demonstrate that the adrenal gland could acetylate the N-terminal serine residue of ACTH failed.

The stimulatory effect of endothelin-1 on regenerating adrenal cortex is reversed by nifedipine

1997 ◽  
Vol 136 (1) ◽  
pp. 121-122
Author(s):  
Wojciech Zieleniewski
Keyword(s):  
Calcium Channels ◽  
Mitotic Index ◽  
Adrenal Cortex ◽  
Plasma Corticosterone ◽  
Zona Glomerulosa ◽  
The Other ◽  
Regulatory Factor ◽  
Adult Rats ◽  
Endothelin 1 ◽  
Potent Vasoconstrictor

Abstract Endothelin-1 (ET-1), a potent vasoconstrictor, was found to act in non-vascular tissues, for example it enhanced aldosterone output from adrenal zona glomerulosa. As the adrenal cortex is capable of regeneration after enucleation, it seemed of interest to study the effects of ET-1 on adrenocortical regeneration. The study was performed on adult rats subjected to left adrenal enucleation combined with contralateral adrenalectomy. Mitotic index was employed to assess the proliferation of regenerating adrenal cortex cells. Plasma corticosterone was measured by a standard RIA kit. ET-1 significantly raised the mitotic index of regenerating rat adrenal cortex by six days after surgery. On the other hand, nifedipine reduced the proliferation ratio and abolished the stimulatory influence of ET-1. Similarly, ET-1 enhanced corticosterone output from the regenerating adrenal cortex, and this could be prevented by the addition of nifedipine. This study has shown that ET-1 might act as a regulatory factor on the regenerating adrenal cortex via calcium channels. European Journal of Endocrinology 136 121–122

scholarly journals Chronic Exposure to Cadmium Disrupts the Adrenal Gland Activity of the NewtTriturus carnifex(Amphibia, Urodela)

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Flaminia Gay ◽  
Vincenza Laforgia ◽  
Ivana Caputo ◽  
Carla Esposito ◽  
Marilena Lepretti ◽  
...  
Keyword(s):  
Drinking Water ◽  
Adrenal Gland ◽  
Human Health ◽  
European Community ◽  
Chronic Exposure ◽  
Adrenocorticotropic Hormone ◽  
Serum Levels ◽  
The Other ◽  
Serum Concentrations ◽  
Ministry Of Health

We intended to verify the safety of the freshwater values established for cadmium by the European Community and the Italian Ministry of Health in drinking water (5 μg/L) and sewage waters (20 μg/L). Therefore, we chronically exposed the newtTriturus carnifexto 5 μg/L and 20 μg/L doses of cadmium, respectively, during 3 and 9 months and verified the effects on the adrenal gland. We evaluated the serum concentrations of adrenocorticotropic hormone (ACTH), corticosterone, aldosterone, norepinephrine, and epinephrine. During the 3-month exposure, both doses of cadmium decreased ACTH and corticosterone serum levels and increased aldosterone and epinephrine serum levels. During the 9-month exposure, the 5 μg/L dose decreased ACTH and increased aldosterone and epinephrine serum levels; the 20 μg/L dose decreased norepinephrine and epinephrine serum levels, without affecting the other hormones. It was concluded that (1) chronic exposure to the safety values established for cadmium disrupted the adrenal gland activity and (2) the effects of cadmium were related both to the length of exposure and the dose administered. Moreover, our results suggest probable risks to human health, due to the use of water contaminated by cadmium.

scholarly journals Эндогенный гиперкортизолизм: достижения и перспективы в диагностике и лечении

2021 ◽  
Vol 23 (3) ◽  
pp. 9-16
Author(s):  
Nicolay A. Maistrenko ◽  
Pavel N. Romashchenko ◽  
Vsevolod Yu. Cherebillo ◽  
Vitaly S. Dovganyuk
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Adrenal Gland ◽  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Resonance Imaging ◽  
Unilateral Adrenalectomy ◽  
Surgical Interventions ◽  
Control Magnetic Resonance Imaging ◽  
3D Volume

The results of examination and treatment of 647 patients with endogenous hypercortisolism were studied: pituitary corticotropinoma was detected in 494 (76.4%) patients, corticosteroma and pre corticosteroma of the adrenal gland in 142 (21.9%), bilateral macro-nodular hyperplasia of the adrenal glands of primary adrenal origin-in 11 (1.7%). Differential diagnosis of clinical forms of endogenous hypercortisolism was based on the assessment of the level of adrenocorticotropic hormone and cortisol, both in peripheral blood, and with selective bilateral blood sampling from the adrenal veins and lower stony sinuses, and the study of the nature of samples with 8 mg of dexamethasone. Topical diagnostics consisted in assessing the state of the adrenal glands and pituitary gland during computed tomography and magnetic resonance imaging with the use of contrast agents, and the use of special software 3D-Volume Rendering Technique allowed optimizing tactical and technical approaches to performing surgical interventions. Of the operated patients with adrenocorticotropic hormone dependent endogenous hypercortisolism, total removal according to the control magnetic resonance imaging was achieved in 92.3% of cases, subtotal in 7.7%. However, hormonal remission was achieved only in 82.4% of cases. All patients with corticosteroma and pre-corticosteroma of the adrenal gland underwent adrenalectomy: in 6 patients by open method, in 136 patients by endovideosurgical method (in 11 patients by laparoscopic method, in 124 patients by retroperitoneoscopic method, and in 1 patient by thoracoscopic transdiaphragmatic adrenalectomy). In all patients, the operation led to recovery. Patients with benign macronodular hyperplasia of the adrenal glands needed conservative treatment with steroidogenesis blockers. Indications for surgical treatment in the volume of unilateral adrenalectomy occurred only in 2 patients.

scholarly journals Clinical Endocrinology: A Review of Adrenal Gland Hormonal and Endocrine Metabolic Disorders.

2018 ◽  
Vol 2 (1) ◽  
pp. 01-03
Author(s):  
Navya K
Keyword(s):  
Adrenal Gland ◽  
Hormonal Regulation ◽  
Small Area ◽  
Metabolic Disorders ◽  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Clinical Endocrinology ◽  
Early Morning ◽  
The Body ◽  
The Brain

Adrenal Gland The adrenal glands are controlled in part by the brain. The hypothalamus, a small area of the brain involved in hormonal regulation, produces corticotropin-releasing hormone (CRH) and vasopressin (also known as antidiuretic hormone). Vasopressin and CRH trigger the pituitary gland to secrete corticotropin (also known as adrenocorticotropic hormone or ACTH), which stimulates the adrenal glands to produce corticosteroids. The renin-angiotensin-aldosterone system, regulated mostly by the kidneys, causes the adrenal glands to produce more or less aldosterone. The body controls the levels of corticosteroids according to need. The levels tend to be much higher in the early morning than later in the day. When the body is stressed, due to illness or otherwise, the levels of corticosteroids increase dramatically.

Histomorphometric Study of the Proportion of Cortex and Medulla of the Adrenal Gland-A Postmortem Study

Mediscope ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Dilruba Siddiqua ◽  
Abu Sadat Mohammad Nurunnabi ◽  
Fatema Johora ◽  
Hasna Hena ◽  
Sunjida Shahriah ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Adrenal Glands ◽  
Age Groups ◽  
Cross Sectional ◽  
Medical College ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Group D

Background: The human adrenal gland shows a distinct proportion of two histological zones, named the cortex and the medulla, from outer inwards, in its histological appearance with differences in its cellular components and functions. Objective: The present study aims to see the variation in the proportion of the cortex and the medulla of the adrenal glands with age in a Bangladeshi population. Methods: A cross-sectional, descriptive study was done in the Department of Anatomy, Dhaka Medical College, Dhaka, from July 2008 to June 2009, based on collection of 140 postmortem human adrenal glands from 70 unclaimed dead bodies, in the Department of Forensic Medicine, Dhaka Medical College, Dhaka. The samples were divided into four age-groups including A (11-20 years), B (21-30 years), C (31-40 years) & D (41-60 years). Histological slides were prepared by using routine haematoxylin and eosin stain (H & E). Five best prepared slides from each group were examined under light compound microscope with low magnification. The thickness of adrenal cortex and medulla were measured by using ocular and stage micrometer and then converted into percentage volume. Results: The mean volume of the right adrenal cortex were found 83.64±3.71% in group A, 83.90±1.75% in group B, 83.74±5.78% in group C, 84.80±3.82% in group D, while the mean volume of the corresponding medulla were found 18.16±3.23% in group A, 15.70±2.71% in group B, 16.26±3.97% in group C, 16.20±4.04% in group D. The mean volume of the left adrenal cortex were found 84.64±3.49%, 84.90±1.75%, 84.20±3.40%, 85.44±2.66% in group A, B, C and D respectively, while the mean volume of the corresponding medulla were found 17.26±3.84%, 17.00±2.37%, 16.00±3.20% and 14.36±2.33% in group A, B, C and D respectively. The differences among the groups were not statistically significant. Conclusion: No difference was found in the proportion of the cortex and the medulla of adrenal gland in different age-groups. Mediscope Vol. 8, No. 1: January 2021, Page 1-6

scholarly journals Intermittent hypoxia improves behavioral and adrenal gland dysfunction induced by posttraumatic stress disorder in rats

2018 ◽  
Vol 125 (3) ◽  
pp. 931-937 ◽  
Author(s):  
Eugenia B. Manukhina ◽  
Vadim E. Tseilikman ◽  
Olga B. Tseilikman ◽  
Maria V. Komelkova ◽  
Marina V. Kondashevskaya ◽  
...  
Keyword(s):  
Posttraumatic Stress Disorder ◽  
Adrenal Gland ◽  
Posttraumatic Stress ◽  
Adrenal Cortex ◽  
Intermittent Hypoxia ◽  
Adrenal Glands ◽  
Stress Disorder ◽  
Morphological Evidence ◽  
Antistress Effect ◽  
Anxiety Index

Nonpharmacological treatments of stress-induced disorders are promising, since they enhance endogenous stress defense systems, are free of side effects, and have few contraindications. The present study tested the hypothesis that intermittent hypoxia conditioning (IHC) ameliorates behavioral, biochemical, and morphological signs of experimental posttraumatic stress disorder (PTSD) induced in rats with a model of predator stress (10-day exposure to cat urine scent, 15 min daily followed by 14 days of stress-free rest). After the last day of stress exposure, rats were conditioned in an altitude chamber for 14 days at a 1,000-m simulated altitude for 30 min on day 1 with altitude and duration progressively increasing to 4,000 m for 4 h on day 5. PTSD was associated with decreased time spent in open arms and increased time spent in closed arms of the elevated X-maze, increased anxiety index, and increased rate of freezing responses. Functional and structural signs of adrenal cortex degeneration were also observed, including decreased plasma concentration of corticosterone, decreased weight of adrenal glands, reduced thickness of the fasciculate zone, and hydropic degeneration of adrenal gland cells. The thickness of the adrenal fasciculate zone negatively correlated with the anxiety index. IHC alleviated both behavioral signs of PTSD and morphological evidence of adrenal cortex dystrophy. Also, IHC alone exerted an antistress effect, which was evident from the increased time spent in open arms of the elevated X-maze and a lower number of rats displaying freezing responses. Therefore, IHC of rats with experimental PTSD reduced behavioral signs of the condition and damage to the adrenal glands. NEW & NOTEWORTHY Intermittent hypoxia conditioning (IHC) has been shown to be cardio-, vaso-, and neuroprotective. For the first time, in a model of posttraumatic stress disorder (PTSD), this study showed that IHC alleviated both PTSD-induced behavioral disorders and functional and morphological damage to the adrenal glands. Also, IHC alone exerted an antistress effect. These results suggest that IHC may be a promising complementary treatment for PTSD-associated disorders.

THE HISTOLOGY OF THE ADRENAL GLAND OF THE PROTOTHERIA

1957 ◽  
Vol 15 (1) ◽  
pp. 100-107 ◽  
Author(s):  
A. WRIGHT ◽  
I. CHESTER JONES ◽  
J. G. PHILLIPS
Keyword(s):  
Adrenal Gland ◽  
Adrenal Cortex ◽  
Adrenal Glands ◽  
Cell Types ◽  
Caudal Part ◽  
Group Iii ◽  
Group I ◽  
Histological Appearance ◽  
Peripheral Cells ◽  
Chromaffin Tissue

SUMMARY The histology of freshly fixed adrenal glands of Ornithorhynchus and of Tachyglossus has been investigated. In the adrenals of both species the bulk of the chromaffin tissue was found to occur at one pole, the lower or caudal part of the gland. The adrenal cortex of Ornithorhynchus has a complicated histological appearance and comprises three main types of tissues, designated groups I-III. The major portion of the cortex was made up of groups II and III tissues which were in contiguous patches associated with blood vessels. Group I tissue was confined, for the most part, to a layer of cells lying against the chromaffin tissue. Group III tissue consisted of big cells with large nuclei which frequently contained prominent globules or vacuoles. It is possible that group III represents the secreting part of the cortex and group II the formative layer. In Tachyglossus, the cortex had a more homogeneous appearance and was more akin to that of reptiles. There was, however, some gradation of cell types. The peripheral cells had plentiful cytoplasm with faintly basophilic nuclei, while centripetally the cells were closely packed and the nuclei densely stained.

scholarly journals The influence of oligopeptides - the homologues of ACTH15-18 on the liver and adrenal glands in the rats on the model of acute immobilization stress

2017 ◽  
Vol 15 (4) ◽  
pp. 30-37
Author(s):  
Olesya V Kudina ◽  
Sergey Yu Shtrygol’ ◽  
Aleksandr A Kolobov ◽  
Yulia B Lar’yanovska
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Adrenal Glands ◽  
Immobilization Stress ◽  
Protective Action ◽  
Antioxidant Effect ◽  
Histological Structure ◽  
Reference Drug ◽  
Depth Study ◽  
Increased Activity ◽  
Positive Effect

An in-depth study the possible links of the stress-protective action of oligopeptides - homologues of the of adrenocorticotropic hormone fragment (15-18) on the model of acute immobilization stress has been carried out. A marked antioxidant effect, not inferior to the reference medicine “Semax”, has been detected in the blood serum and liver of the rats. The results of the morphological study of liver tissue also indicate the normalization of the stress-induced damage in the organ under the influence of oligopeptides (KK-1, KK-5). Both peptides have been exceeded the reference drug, however, the peptide KK-1 had the marked positive effect on the reduction of histological structure of the liver. The stress-protective action of the investigated oligopeptides KK-1 and KK-5 is confirmed by their ability to reduce the increased activity of steroidogenesis and reduce the release of catecholamines, as well as normalize the damaged structure of the adrenal cortex. According to the indices of the adrenal structure recovering, the peptide KK-1 exceeded the reference drug and the peptide KK-1. (For citation: Kudina OV, Shtrygol’ SYu, Kolobov AA, Larjanovskaja YuB, et al. The influence of oligopeptides - the homologues of ACTH15-18 on the liver and adrenal glands in the rats on the model of acute immobilization stress. Reviews on Clinical Pharmacology and Drug Therapy. 2017;15(4):30-37. doi: 10.17816/RCF15430-37).

Adrenal Glands

2020 ◽  
pp. 1-14
Author(s):  
Jad M. Abdelsattar ◽  
Moustafa M. El Khatib ◽  
T. K. Pandian ◽  
Samuel J. Allen ◽  
David R. Farley
Keyword(s):  
Adrenal Gland ◽  
Neural Crest ◽  
Blood Vessels ◽  
Adrenal Cortex ◽  
Sex Hormones ◽  
Laparoscopic Adrenalectomy ◽  
Adrenal Glands ◽  
Adrenal Incidentalomas

The adrenal cortex is derived from mesoderm and the medulla, from neural crest ectoderm. An adrenal gland weighs approximately 5 g. The cortex secretes corticosteroids (cortisol), aldosterone, and sex hormones. Masses are most commonly asymptomatic. Adrenal incidentalomas are masses greater than 1 cm in diameter that are found in 2% to 4% of all CTs. Adrenalectomy is indicated for masses that are enlarging, larger than 4 to 6 cm, or functional (secreting hormone); the operation is fixated on safe transection of adrenal blood vessels. Complications are relatively uncommon with laparoscopic adrenalectomy.

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