The Influence of Testosterone on the Endogenous Levels of Prostaglandin F in the Accessory Reproductive Glands of the Adult Male Rat

1974 ◽  
Vol 52 (2) ◽  
pp. 364-367 ◽  
Author(s):  
D. J. B. Sutherland ◽  
A. H. Telli ◽  
R. L. Singhal
Keyword(s):  
Free Testosterone ◽  
Hormone Treatment ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
Male Rat ◽  
Adult Rats ◽  
Intramuscular Dose ◽  
Accessory Reproductive Glands ◽  
Adult Male Rat ◽  
Prostaglandin F

Orchidectomy increased the endogenous concentration of prostaglandin F (PGF) in the prostatic and vesicular glands of adult rats. A single intramuscular dose of free testosterone (5.0 mg/100 g) was able to reverse the effects of castration on PGF concentration of the accessory reproductive organs. In the case of seminal vesicles, administration of testosterone to castrate rats markedly reduced the PGF levels within 144 h of hormone treatment.

Acid phosphatases: androgen dependent markers of rat prostate

1976 ◽  
Vol 54 (4) ◽  
pp. 350-357 ◽  
Author(s):  
M. Tenniswood ◽  
C. E. Bird ◽  
A. F. Clark
Keyword(s):  
Blood Cells ◽  
Gel Electrophoresis ◽  
Significant Inhibition ◽  
Seminal Vesicles ◽  
The Other ◽  
Male Rat ◽  
Rat Tissues ◽  
Testosterone Enanthate ◽  
Adult Male Rat ◽  
Rat Prostate

Our investigations on acid phosphatase (AP) were aimed at finding a biochemical assay marker for androgen actions in the rat prostate. We quantitatively examined the effects of l-tartrate or formaldehyde on AP activity in tissue filtrates from nine adult male rat tissues, plasma and hemolysed red blood cells (HRBC). There was significant inhibition of AP activity in all instances with the exception of HRBC with tartrate. The prostate inhibition results were not different from those for seminal vesicles and adrenals but were different from the other tissues studied. Ten days following castration the inhibition by tartrate was less in all tissues studied except plasma and HRBC; the formaldehyde inhibition percentages were not altered. Intraperitoneal testosterone enanthate administration begun 2 days after castration maintained the tartrate inhibitions in the ranges found for noncastrated rats. Gel electrophoresis of the tissue filtrates and staining of the gels for AP indicated two bands of AP activity for the prostate from normal rats and one band of activity for all other tissues. This second band of prostate AP activity was completely eliminated by the addition of formaldehyde and was not found for prostate tissue filtrates from castrated animals. However, it was found for the animals which had received testosterone replacement for 14 days. It would appear that AP can be used as a marker of androgen responsiveness for rat prostate.

Presence of α-glucosidases in the male reproductive system of the rat and hormonal influences

1983 ◽  
Vol 61 (7) ◽  
pp. 764-769 ◽  
Author(s):  
Anne-Marie Grandmont ◽  
Pierre Chapdelaine ◽  
Roland R. Tremblay
Keyword(s):  
Enzyme Activity ◽  
Reproductive System ◽  
Reproductive Organs ◽  
Gonadal Hormones ◽  
Antagonistic Effect ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
Male Rat ◽  
Glucosidase Activity ◽  
Estradiol 17Β

The use of a synthetic substrate (p-nitrophenyl-α-D-glucoside) to measure α-glucosidase activity has allowed us to demonstrate the presence of acid and neutral α-glucosidases in the reproductive organs of the male rat. Both enzymes increased in the epididymis, particularly in the caput segment, along with initiation of spermatogenesis at puberty; it then started decreasing after 12 weeks of life. Similar variations were not recorded in testis, prostate, and seminal vesicles. Castration led to a significant decrease of acid and neutral α-glucosidases in all accessory reproductive organs, but administration of testosterone proprionate (50 μg/day for 10 days) restored the enzyme activity to its original level. When estradiol-17β (5 mg) was administered simultaneously with testosterone (500 μg), the antagonistic effect of estradiol on testosterone was particularly evident on the levels of neutral α-glucosidases which reached the castration range, while the acid α-glucosidase remained unchanged in epididymis, prostate, and seminal vesicles. These results show that both acid and neutral α-glucosidases may be influenced by gonadal hormones in the male rat.

scholarly journals Detection of α2u-globulin in rat pup preputial gland by MALDI-TOF mass spectrometry

2009 ◽  
Vol 55 (4) ◽  
pp. 296-300 ◽  
Author(s):  
Ponnirul Ponmanickam ◽  
Gnanasekaran Jebamercy ◽  
Govindaraju Archunan ◽  
Soundrapandian Kannan
Keyword(s):  
Mass Spectrometry ◽  
Male Rat ◽  
Preputial Gland ◽  
Adult Rats ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Pheromonal Communication ◽  
Adult Male Rat ◽  
Prepubertal Rats ◽  
Preputial Glands

Abstract The α2u-globulin, a soluble protein identified in the urine and preputial gland of adult male rat is reported to be pheromone carrier. The pup preputial gland plays a significant role in chemical communication for mother-young interaction; however, the presence of a pheromone-carrying protein in the pup preputial gland has not been confirmed. Therefore, the present study was carried out to identify the α2u-globulin in the pup preputial gland by Matrix Assisted Laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF). The preputial glands of prepubertal rats were subjected to one-dimensional SDS-PAGE. In-gel trypsin digestion of a 18 kDa band was carried out and analyzed by MALDI-TOF. The results of a MASCOT search showed the presence of α2u-globulin in the 18 kDa band. In contrast to the report of the synthesis of this protein only in adult rats, the identification of this protein in pup preputial gland is significant. The results suggest that synthesis of α2u-globulin in the rat preputial gland starts in the prepubertal stage itself. In prepubertal rats, the preputial gland is a source of pheromone for performing anogenital licking behaviour by the mother rat. Since α2u-globulin belongs to the lipocalin (ligand carrier) family, it might carry the volatile for processing pheromonal communication in mother-pup bonding in rat.

scholarly journals Anatomy and Physiology of Animal Model Rats in Biomedical Research

2021 ◽  
Vol 7 (2) ◽  
pp. 265-269
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Seminal Vesicles ◽  
Male Rat ◽  
Female Rat ◽  
Anogenital Distance ◽  
Anatomy And Physiology ◽  
Sperm Counts ◽  
Yellow Orange ◽  
Adult Male Rat ◽  
Preputial Glands ◽  
Orange Color

A distinguishing feature of rodents, including rats, is the absence of canines and thepresence of prominent incisors. Rats are monophydontic, meaning they grow one setof teeth in their lifetime. The enamel of the rodent incisor contains iron, which givesit its yellow-orange color. Rats are mammals and as such, possess many similaritieswith other mammals. Only the peculiarities of the rat’s anatomy are addressed. Malerats reach puberty at 40 - 60 days of age. Descent of the testes usually occursbetween days 30 - 60. Sperm counts vary by strain. The male rat has an os penis.Male rats have the following accessory sexual organs: ampulla, seminal vesicles,prostate, bulbourethral glands, coagulating glands, and preputial glands. Thecoagulating gland and prostatic and vesicular secretions are responsible for thecopulation plug, a firm plug deposited in the vagina of the female after copulation.(This plug, when found outside the female rat, is capsuleshaped and approximately5 mm long.) The male rat has no nipples. The adult male rat has a prominentscrotum and a longer anogenital distance than the female rat.

PITUITARY REGULATION OF LEYDIG CELL FUNCTION IN THE ADULT MALE RAT

1977 ◽  
Vol 74 (1) ◽  
pp. 57-66 ◽  
Author(s):  
R. L. HAUGER ◽  
Y.-D. I. CHEN ◽  
R. P. KELCH ◽  
A. H. PAYNE
Keyword(s):  
Cell Function ◽  
Binding Capacity ◽  
Specific Binding ◽  
Intraperitoneal Administration ◽  
Serum Testosterone ◽  
Pituitary Hormones ◽  
Male Rat ◽  
Scatchard Analysis ◽  
Adult Rats ◽  
Adult Male Rat

The effects of hypophysectomy on serum testosterone, 125I-labelled hCG binding to testicular membranes and on testicular responsiveness were studied in adult rats. Serum testosterone decreased rapidly over the first 6 h after hypophysectomy. LH receptors were determined (pmol/testis) by measuring the specific binding of 125I-labelled hCG in membrane preparations of testes of rats hypophysectomized 1, 2, 3, 6, 9, or 15 days earlier. Hypophysectomy did not result in a decrease in 125I-labelled hCG binding on day 1 but this had decreased to 40% of that in intact controls by day 2. A gradual decline was found between days 2 and 6 at which time hCG binding had decreased to 15%. No further decrease occurred between days 6 and 15. Scatchard analysis indicated that the decline in hCG binding was due to a decrease in binding capacity and not to a decrease in binding affinity. FSH, testosterone, dihydrotestosterone, and oestradiol were unable to prevent the decline in hCG binding. Although serum testosterone, testicular testosterone content, and 125I-labelled hCG binding decreased rapidly after hypophysectomy, testicular responsiveness to LH was biphasic. The intraperitoneal administration of 25 μg LH 2 h before decapitation increased testosterone in the circulation to a greater extent in animals hypophysectomized for 1 day than in intact controls while hCG binding affinities and capacities had not changed. Two or three days after hypophysectomy testicular responsiveness to LH was similar to that of intact controls even though hCG binding in hypophysectomized animals had decreased to 40 and 28% of intact controls respectively. It is concluded that (1) the testis is dependent on anterior pituitary hormones for maintenance of testicular LH receptors and testosterone secretion, (2) FSH, testosterone, dihydrotestosterone, or oestradiol cannot prevent the decline in testicular LH receptors resulting from hypophysectomy, and (3) steroidogenic capacity of the testis persists significantly longer than the hCG binding capacity of the testis.

EFFECTS OF TESTOSTERONE PROPIONATE, 5α-DIHYDROTESTOSTERONE PROPIONATE AND OESTRADIOL BENZOATE ON SERUM LEVELS OF LH AND FSH IN THE CASTRATED ADULT MALE RAT

1974 ◽  
Vol 77 (4) ◽  
pp. 643-654 ◽  
Author(s):  
H. L. Verjans ◽  
K. B. Eik-Nes ◽  
J. H. Aafjes ◽  
F. J. M. Vels ◽  
H. J. van der Molen
Keyword(s):  
Adult Male ◽  
Testosterone Propionate ◽  
Serum Levels ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Male Rat ◽  
Low Doses ◽  
Serum Lh ◽  
Oestradiol Benzoate ◽  
Adult Male Rat

ABSTRACT The influence of treatment with various doses of testosterone propionate, 5α-dihydrotestosterone propionate or oestradiol benzoate on serum levels of LH and FSH (measured by radioimmunoassay) and on weights of ventral prostates and seminal vesicles was investigated in castrated, adult, male rats. For depression of the high, castrate levels of serum gonadotrophins with either of these steroid esters, the inhibition curves were different for LH and for FSH. Serum LH was kept at levels encountered in intact, adult, male rats by lower doses of steroid ester than was serum FSH. Oestradiol benzoate was the most potent suppressor of the serum gonadotrophins among the steroid esters tested, testosterone propionate the least. Treatment with low doses of oestradiol benzoate, however, resulted in serum FSH levels significantly above those of castrates treated with vehicle only. Finally, administration of a synthetic LH-releasing factor to testosterone propionate, 5α-dihydrotestosterone propionate or oestradiol benzoate treated, castrated, adult, male rats resulted in a further release of both LH and FSH. The latter effect was more pronounced in oestradiol benzoate treated castrates than in testosterone propionate or 5α-dihydrotestosterone propionate treated castrates.

scholarly journals Effects of testosterone on the metabolism of folate coenzymes in the rat

1972 ◽  
Vol 126 (2) ◽  
pp. 291-294 ◽  
Author(s):  
C. Rovinetti ◽  
C. Bovina ◽  
B. Tolomelli ◽  
M. Marchetti
Keyword(s):  
Control Mechanism ◽  
Normal Values ◽  
Hormone Treatment ◽  
Seminal Vesicles ◽  
Serine Hydroxymethyltransferase ◽  
Adult Rats ◽  
Male Adult ◽  
Testosterone Treatment ◽  
Accessory Sex Organs ◽  
Methylenetetrahydrofolate Dehydrogenase

1. The effects of castration and testosterone treatment on enzymic activities involved in folate coenzyme metabolism in the liver and in accessory sex organs of male adult rats were studied. 2. In the liver of castrated rats the concentration of 10-formyltetrahydrofolate (10-HCO-H4folate) synthetase and tetrahydrofolate (H4folate) dehydrogenase were significantly decreased whereas that of 5,10-methylenetetrahydrofolate dehydrogenase increased; the treatment with five doses of testosterone caused a return to normal values of these activities. 3. In the prostate of castrated rats a pronounced decrease in H4folate dehydrogenase, serine hydroxymethyltransferase and 10-HCO-H4folate synthetase activities was observed. The administration of testosterone restored the enzymic activities to normal values. 4. In the seminal vesicles of castrated rats only 10-HCO-H4folate synthetase was markedly depressed; testosterone treatment not only restored activity to normal values but raised it to higher than normal values. The slight changes observed in other enzymic activities also returned to normal values with the hormone treatment. 5. These results are discussed in relation to a possible control mechanism of folate metabolism by testosterone.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

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