Acid phosphatases: androgen dependent markers of rat prostate

1976 ◽  
Vol 54 (4) ◽  
pp. 350-357 ◽  
Author(s):  
M. Tenniswood ◽  
C. E. Bird ◽  
A. F. Clark
Keyword(s):  
Blood Cells ◽  
Gel Electrophoresis ◽  
Significant Inhibition ◽  
Seminal Vesicles ◽  
The Other ◽  
Male Rat ◽  
Rat Tissues ◽  
Testosterone Enanthate ◽  
Adult Male Rat ◽  
Rat Prostate

Our investigations on acid phosphatase (AP) were aimed at finding a biochemical assay marker for androgen actions in the rat prostate. We quantitatively examined the effects of l-tartrate or formaldehyde on AP activity in tissue filtrates from nine adult male rat tissues, plasma and hemolysed red blood cells (HRBC). There was significant inhibition of AP activity in all instances with the exception of HRBC with tartrate. The prostate inhibition results were not different from those for seminal vesicles and adrenals but were different from the other tissues studied. Ten days following castration the inhibition by tartrate was less in all tissues studied except plasma and HRBC; the formaldehyde inhibition percentages were not altered. Intraperitoneal testosterone enanthate administration begun 2 days after castration maintained the tartrate inhibitions in the ranges found for noncastrated rats. Gel electrophoresis of the tissue filtrates and staining of the gels for AP indicated two bands of AP activity for the prostate from normal rats and one band of activity for all other tissues. This second band of prostate AP activity was completely eliminated by the addition of formaldehyde and was not found for prostate tissue filtrates from castrated animals. However, it was found for the animals which had received testosterone replacement for 14 days. It would appear that AP can be used as a marker of androgen responsiveness for rat prostate.

scholarly journals Anatomy and Physiology of Animal Model Rats in Biomedical Research

2021 ◽  
Vol 7 (2) ◽  
pp. 265-269
Author(s):  
Rachmat Hidayat ◽  
Patricia Wulandari
Keyword(s):  
Seminal Vesicles ◽  
Male Rat ◽  
Female Rat ◽  
Anogenital Distance ◽  
Anatomy And Physiology ◽  
Sperm Counts ◽  
Yellow Orange ◽  
Adult Male Rat ◽  
Preputial Glands ◽  
Orange Color

A distinguishing feature of rodents, including rats, is the absence of canines and thepresence of prominent incisors. Rats are monophydontic, meaning they grow one setof teeth in their lifetime. The enamel of the rodent incisor contains iron, which givesit its yellow-orange color. Rats are mammals and as such, possess many similaritieswith other mammals. Only the peculiarities of the rat’s anatomy are addressed. Malerats reach puberty at 40 - 60 days of age. Descent of the testes usually occursbetween days 30 - 60. Sperm counts vary by strain. The male rat has an os penis.Male rats have the following accessory sexual organs: ampulla, seminal vesicles,prostate, bulbourethral glands, coagulating glands, and preputial glands. Thecoagulating gland and prostatic and vesicular secretions are responsible for thecopulation plug, a firm plug deposited in the vagina of the female after copulation.(This plug, when found outside the female rat, is capsuleshaped and approximately5 mm long.) The male rat has no nipples. The adult male rat has a prominentscrotum and a longer anogenital distance than the female rat.

EFFECTS OF TESTOSTERONE PROPIONATE, 5α-DIHYDROTESTOSTERONE PROPIONATE AND OESTRADIOL BENZOATE ON SERUM LEVELS OF LH AND FSH IN THE CASTRATED ADULT MALE RAT

1974 ◽  
Vol 77 (4) ◽  
pp. 643-654 ◽  
Author(s):  
H. L. Verjans ◽  
K. B. Eik-Nes ◽  
J. H. Aafjes ◽  
F. J. M. Vels ◽  
H. J. van der Molen
Keyword(s):  
Adult Male ◽  
Testosterone Propionate ◽  
Serum Levels ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Male Rat ◽  
Low Doses ◽  
Serum Lh ◽  
Oestradiol Benzoate ◽  
Adult Male Rat

ABSTRACT The influence of treatment with various doses of testosterone propionate, 5α-dihydrotestosterone propionate or oestradiol benzoate on serum levels of LH and FSH (measured by radioimmunoassay) and on weights of ventral prostates and seminal vesicles was investigated in castrated, adult, male rats. For depression of the high, castrate levels of serum gonadotrophins with either of these steroid esters, the inhibition curves were different for LH and for FSH. Serum LH was kept at levels encountered in intact, adult, male rats by lower doses of steroid ester than was serum FSH. Oestradiol benzoate was the most potent suppressor of the serum gonadotrophins among the steroid esters tested, testosterone propionate the least. Treatment with low doses of oestradiol benzoate, however, resulted in serum FSH levels significantly above those of castrates treated with vehicle only. Finally, administration of a synthetic LH-releasing factor to testosterone propionate, 5α-dihydrotestosterone propionate or oestradiol benzoate treated, castrated, adult, male rats resulted in a further release of both LH and FSH. The latter effect was more pronounced in oestradiol benzoate treated castrates than in testosterone propionate or 5α-dihydrotestosterone propionate treated castrates.

The Influence of Testosterone on the Endogenous Levels of Prostaglandin F in the Accessory Reproductive Glands of the Adult Male Rat

1974 ◽  
Vol 52 (2) ◽  
pp. 364-367 ◽  
Author(s):  
D. J. B. Sutherland ◽  
A. H. Telli ◽  
R. L. Singhal
Keyword(s):  
Free Testosterone ◽  
Hormone Treatment ◽  
Reproductive Organs ◽  
Seminal Vesicles ◽  
Male Rat ◽  
Adult Rats ◽  
Intramuscular Dose ◽  
Accessory Reproductive Glands ◽  
Adult Male Rat ◽  
Prostaglandin F

Orchidectomy increased the endogenous concentration of prostaglandin F (PGF) in the prostatic and vesicular glands of adult rats. A single intramuscular dose of free testosterone (5.0 mg/100 g) was able to reverse the effects of castration on PGF concentration of the accessory reproductive organs. In the case of seminal vesicles, administration of testosterone to castrate rats markedly reduced the PGF levels within 144 h of hormone treatment.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

scholarly journals Maternal tobacco smoke increased visceral adiposity and serum corticosterone levels in adult male rat offspring

2014 ◽  
Vol 76 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Erin K. Zinkhan ◽  
Brook Y. Lang ◽  
Baifeng Yu ◽  
Yan Wang ◽  
Chengshe Jiang ◽  
...  
Keyword(s):  
Adult Male ◽  
Tobacco Smoke ◽  
Visceral Adiposity ◽  
Male Rat ◽  
Serum Corticosterone ◽  
Rat Offspring ◽  
Adult Male Rat

Role of erythrocytes and platelets in the hypercoagulable status in polycythemia vera through phosphatidylserine exposure and microparticle generation

2013 ◽  
Vol 109 (06) ◽  
pp. 1025-1032 ◽  
Author(s):  
Chunyan Gao ◽  
Xue Yang ◽  
Jianan Li ◽  
Wei Wang ◽  
Jinxiao Hou ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
Significant Inhibition ◽  
Polycythaemia Vera ◽  
Factor V ◽  
Healthy Controls ◽  
Clotting Time ◽  
Thrombotic Risk ◽  
Few Data

SummaryThe development of thrombosis in polycythaemia vera (PV) involves multifactorial processes including pathological activation of blood cells. Release of microparticles (MPs) by activated cells in diseases is associated with thrombotic risk, but relatively few data are available in PV. The aim of the present study was to investigate the increase in MP release and exposure of phosphatidylserine (PS) on the outer membrane of MP-origin cells in patients with PV, and to analyse their procoagulant activity (PCA). PS-positive MPs and cells were detected by flow cytometry, while PCA was assessed with clotting time and purified coagulation complex assays. We found that PV patients had elevated circulating lactadherin+ MPs, which mostly originating from erythrocytes, platelets, granulocytes, and endothelial cells, as well as increased PS exposing erythrocytes/platelets as compared to secondary polycythaemia patients or healthy controls. These PS-bearing MPs and cells were highly procoagulant. Moreover, lactadherin competed factor V and VIII to PS and inhibited about 90% of the detected PCA in a dose-response manner while anti-TF antibody did no significant inhibition. Treatment with hydroxyurea is associated with a decrease in PS exposure and lactadherin+ MP release of erythrocytes/platelets. Our data demonstrate that PV patients are characterised by increased circulating procoagulant MPs and PS exposing erythrocytes/platelets, which could contribute to the hypercoagulable state in these patients.

15-Day intact adult male rat assay with alpha-cypermethrin

2016 ◽  
Vol 258 ◽  
pp. S308-S309 ◽  
Author(s):  
M. Kemény ◽  
H.A. Marxfeld ◽  
R. Buesen ◽  
A. Chukwudebe ◽  
B. Van Ravenzwaay ◽  
...  
Keyword(s):  
Adult Male ◽  
Male Rat ◽  
Adult Male Rat
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