FORMATION OF STEROID HORMONES IN THE ADRENAL ADENOMA AND ADJACENT ADRENAL TISSUE OF A PATIENT WITH PRIMARY ALDOSTERONISM

1963 ◽  
Vol 41 (1) ◽  
pp. 1955-1959
Author(s):  
Elizabeth MacArthur ◽  
V. J. O'Donnell
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Primary Aldosteronism ◽  
Incubation Medium ◽  
Adrenal Adenoma ◽  
Adrenal Vein ◽  
Adrenal Tissue

The in vitro steroidogenic capacity of an adrenal adenoma and adjacent adrenal tissue of a patient with primary aldosteronism was studied. The adenoma slices elaborated into the incubation medium 7.07 μg/g tissue of cortisol (78.2%), corticosterone (7.6%), and aldosterone (14.2%), while the slices of adjacent adrenal gland released 19.88 μg/g tissue of cortisol (79.1%), corticosterone (14.9%), 11β-hydroxyandrostenedione (3.9%), androstenedione (2.1%), and a trace of aldosterone. The steroid content of the adenoma and the adrenal slices after incubation was 4.22 and 3.19 μg/g tissue respectively. From the former, cortisol (51.1%), corticosterone (36.6%), and progesterone (12.4%) were isolated and from the latter cortisol (13.2%), corticosterone (56.1%), progesterone (17.2%), and androstenedione (13.5%). A sample of adrenal vein blood obtained prior to adrenalectomy exhibited a cortisol/corticosterone ratio of 2.45.

FORMATION OF STEROID HORMONES IN THE ADRENAL ADENOMA AND ADJACENT ADRENAL TISSUE OF A PATIENT WITH PRIMARY ALDOSTERONISM

1963 ◽  
Vol 41 (9) ◽  
pp. 1955-1959 ◽  
Author(s):  
Elizabeth MacArthur ◽  
V. J. O'Donnell
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Primary Aldosteronism ◽  
Incubation Medium ◽  
Adrenal Adenoma ◽  
Adrenal Vein ◽  
Adrenal Tissue

The in vitro steroidogenic capacity of an adrenal adenoma and adjacent adrenal tissue of a patient with primary aldosteronism was studied. The adenoma slices elaborated into the incubation medium 7.07 μg/g tissue of cortisol (78.2%), corticosterone (7.6%), and aldosterone (14.2%), while the slices of adjacent adrenal gland released 19.88 μg/g tissue of cortisol (79.1%), corticosterone (14.9%), 11β-hydroxyandrostenedione (3.9%), androstenedione (2.1%), and a trace of aldosterone. The steroid content of the adenoma and the adrenal slices after incubation was 4.22 and 3.19 μg/g tissue respectively. From the former, cortisol (51.1%), corticosterone (36.6%), and progesterone (12.4%) were isolated and from the latter cortisol (13.2%), corticosterone (56.1%), progesterone (17.2%), and androstenedione (13.5%). A sample of adrenal vein blood obtained prior to adrenalectomy exhibited a cortisol/corticosterone ratio of 2.45.

UTILIZATION OF [26-14C]CHOLESTEROL BY HUMAN FOETAL ADRENAL GLANDS IN VITRO

1978 ◽  
Vol 79 (3) ◽  
pp. 393-394 ◽  
Author(s):  
R. GUNASEGARAM ◽  
K. L. PEH ◽  
P. C. T. CHEW ◽  
S. M. M. KARIM ◽  
S. S. RATNAM
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Adrenal Glands ◽  
De Novo ◽  
Obstetrics And Gynaecology ◽  
Perfusion Studies

Department of Obstetrics and Gynaecology, University of Singapore, Kandang Kerbau Hospitalfor Women, Singapore 8, Republic of Singapore (Received 3 May 1978) From the previous studies of Bloch & Benirschke (1959, 1962) and Plotz, Kabara, Davis, LeRoy & Gould (1968) it appears that at mid-term, human foetal adrenal glands are capable of synthesizing C21- and C19-steroids de novo from acetate and cholesterol. Villee, Engel, Loring & Villee (1961), however, incubated slices and homogenates of foetal adrenal gland with [2-14C]acetate or [4-14C]cholesterol and could not demonstrate the incorporation of radioactivity into these steroids. Moreover, perfusion studies by three groups of investigators indicated only minute conversions of the same radioactive substrates into neutral steroids in the foetal adrenal glands (Solomon, Bird, Ling, Iwamiya & Young, 1967; Telegdy, Weeks, Archer, Wiqvist & Diczfalusy, 1970a; Telegdy, Weeks, Lerner, Stakemann & Diczfalusy, 1970b). It is widely believed that steroid hormones are normally synthesized from acetate via

EFFECTS OF HYDROXYSTEROID DEHYDROGENASE INHIBITORS ON IN-VITRO AND IN-VIVO STEROIDOGENESIS IN THE OVINE ADRENAL GLAND

1982 ◽  
Vol 92 (2) ◽  
pp. 205-212 ◽  
Author(s):  
P. SINGH-ASA ◽  
G. JENKIN ◽  
G. D. THORBURN
Keyword(s):  
Adrenal Gland ◽  
Incubation Medium ◽  
Bovine Serum ◽  
Hydroxysteroid Dehydrogenase ◽  
Inhibitory Effect ◽  
Bovine Serum Albu ◽  
Stimulatory Action ◽  
Adrenal Steroidogenesis

The effectiveness of trilostane and azastene as inhibitors of adrenal steroidogenesis was compared by in-vitro and in-vivo methods. A radioimmunoassay was developed for the measurement of cortisol in ovine plasma, incubation medium and tissue extract using a specific antiserum raised against cortisol 21-acetate,3-carboxymethyloxime : bovine serum albu Trilostane (20 μmol/l) decreased cortisol synthesis and release both in unstimulated and in ACTH-stimulated adrenal tissues in vitro. The same concentration of azastene had a lesser effect on unstimulated adrenals and was completely ineffective in blocking the stimulatory action of ACTH. In vivo, trilostane suppressed adrenal steroidogenesis in pregnant and cyclic ewes but the suppression in pregnant ewes was over a longer period, and after lower doses. It is concluded that trilostane had an inhibitory effect on ovine adrenal steroidogenesis both in vitro and in vivo.

Concurrent adrenocortical carcinoma and Conn's adenoma in a man with primary hyperaldosteronism. In vivo and in vitro studies

1992 ◽  
Vol 127 (2) ◽  
pp. 189-192 ◽  
Author(s):  
Y Touitou ◽  
A Boissonnas ◽  
A Bogdan ◽  
A Auzéby
Keyword(s):  
Adrenal Gland ◽  
Adrenocortical Carcinoma ◽  
Primary Aldosteronism ◽  
Unusual Case ◽  
In Vitro Studies ◽  
In Vitro Biosynthesis ◽  
The Right ◽  
Tomography Scan

This is a report of a rare and unusual case of adrenal pathology. A patient presented with clinical and biological signs of primary aldosteronism and computed body tomography scan led to our suspecting the presence of a left adrenocortical carcinoma. The in vitro studies performed on the resected tumour showed very low synthesis of mineralocorticoids and glucocorticoids. The patient could not be re-examined until 15 months later, when he still suffered hypertension; another tomography scan revealed a mass on the right adrenal gland. The studies performed on this second tumour confirmed the diagnosis of Conn's adenoma: active in vitro biosynthesis of 18-hydroxy-corticosterone and aldosterone from exogenous tritiated precursors.

THE ISOLATION OF 6β-HYDROXYCORTICOSTERONE AND ITS 11-DEHYDRO DERIVATIVE FROM THE ADRENAL GLAND, ADRENAL ADENOMA, AND URINE OF A PATIENT WITH PRIMARY HYPERALDOSTERONISM

1962 ◽  
Vol 40 (12) ◽  
pp. 1779-1796 ◽  
Author(s):  
Wojciech Nowaczynski ◽  
Erich Koiw ◽  
Jacques Genest
Keyword(s):  
Adrenal Gland ◽  
Adrenal Adenoma ◽  
Primary Hyperaldosteronism ◽  
Adrenal Tissue ◽  
First Time

Isolation of large amounts of 6β-hydroxycorticosterone from incubates of human hyperplastic and adenomatous adrenal tissue from a patient with primary hyperaldosteronism is described for the first time.(1) Isolation of 11β-hydroxy-Δ4-androstene-3,17-dione from the incubates of the same adenoma is reported.(3) From urines of the above patient and urines of a second case of primary hyperaldosteronism, 6β-hydroxycorticosterone and 6β-hydroxy-11-dehydrocorticosterone were isolated.

A COMPARISON OF THE CONVERSION IN VITRO OF PREGNENOLONE SULPHATE AND PREGNENOLONE TO STEROID HORMONES BY TISSUE FROM A CLEAR CELL ADENOMA OF THE ADRENAL GLAND

1968 ◽  
Vol 40 (1) ◽  
pp. 49-58 ◽  
Author(s):  
K. GRIFFITHS ◽  
D. CUNNINGHAM ◽  
E. H. D. CAMERON
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Adrenal Cortex ◽  
Clear Cell ◽  
Glucose Solution ◽  
Cell Adenoma ◽  
Free Steroid

SUMMARY Tissue from a clear cell adenoma of the adrenal gland was incubated simultaneously with [7α-3H]pregnenolone sulphate and [4-14C]pregnenolone in Krebs—Ringer bicarbonate-glucose solution. Evidence was obtained for the conversion of pregnenolone sulphate to cortisol and corticosterone. The differences in the ability of the tissue to convert the sulphate and the free steroid to corticosteroids and androgens are discussed in relation to theories on the functional zonation of the human adrenal cortex.

PARALLEL BIOCHEMICAL AND HISTOCHEMICAL STUDIES OF AN ADRENOCORTICAL ADENOMA FROM A PATIENT WITH PRIMARY ALDOSTERONISM

1961 ◽  
Vol 38 (2) ◽  
pp. 207-219 ◽  
Author(s):  
Jean Davignon ◽  
Gilles Tremblay ◽  
Wojciech Nowaczynski ◽  
Erich Koiw ◽  
Jacques Genest
Keyword(s):  
Primary Aldosteronism ◽  
Zona Glomerulosa ◽  
Zona Fasciculata ◽  
Morphological Aspect ◽  
Adrenocortical Adenoma ◽  
Acth Stimulation ◽  
Chromatographic Techniques ◽  
Striking Increase ◽  
Adrenal Tissue

ABSTRACT An adrenocortical adenoma surgically removed from a patient with primary aldosteronism was investigated by histological, histochemical and incubation-chromatographic techniques and compared to intact adrenal tissue excised from the contralateral gland. The tumour was composed almost entirely of fasciculata-like tissue and released »in vitro« cortisol, corticosterone, aldosterone, cortisone and 17-hydroxy-11-deoxycorticosterone but no measurable amounts of 11β-hydroxy-androstenedione. In contrast to the contralateral gland with prominent zona glomerulosa and atrophic zona fasciculata, which responded poorly to corticotrophin (ACTH) stimulation, the tumour responded by a striking increase in the formation of cortisol, corticosterone and aldosterone. An attempt was made to correlate the morphological aspect with the biochemical findings »in vitro«.

Childhood Primary Aldosteronism Due to an Adrenal Adenoma: Preoperative Localization by Adrenal Vein Catheterization

PEDIATRICS ◽  
1980 ◽  
Vol 65 (3) ◽  
pp. 605-609 ◽  
Author(s):  
Arunabha Ganguly ◽  
Jerry Bergstein ◽  
Clarence E. Grim ◽  
Moo Nahm Yum ◽  
Myron H. Weinberger
Keyword(s):  
Plasma Renin Activity ◽  
Primary Aldosteronism ◽  
Incidental Findings ◽  
Adrenal Adenoma ◽  
Plasma Renin ◽  
Adrenal Vein ◽  
Pathologic Examination ◽  
Surgical Exploration ◽  
Vein Catheterization ◽  
Biochemical Abnormalities

Primary aldosteronism resulting from an adrenal adenoma is rare in children. An 8½-year-old girl was found to have hypertension and spontaneous hypokalemia, both detected as incidental findings. Subsequent investigations revealed inappropriately elevated levels of plasma and urinary aldosterone with suppressed plasma renin activity. Adrenal vein blood sampling and venography suggested the presence of left adrenal adenoma which was confirmed at surgical exploration and pathologic examination. All the clinical and biochemical abnormalities were corrected by the adrenalectomy. The differential diagnoses of various types of hyperaldosteronism and/or hypokalemia in such a clinical setting are discussed.

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