THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (1) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (3) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

PROPERTIES OF CALF THYMUS HISTONE ISOLATED BY THREE DIFFERENT METHODS

1958 ◽  
Vol 36 (1) ◽  
pp. 1-13 ◽  
Author(s):  
L. B. Smillie ◽  
G. C. Butler ◽  
David B. Smith
Keyword(s):  
Nucleic Acid ◽  
Hydrochloric Acid ◽  
Aqueous Solutions ◽  
Sedimentation Rate ◽  
Principal Components ◽  
Dilute Hydrochloric Acid ◽  
Original Material ◽  
Salting Out ◽  
Calf Thymus ◽  
Two Stages

Histone of calf thymus deoxyribonucleoprotein was isolated by three different methods: (1) Extraction from nucleoprotein solutions with dilute hydrochloric acid. (2) Extraction from cold saline nucleoprotein solutions with ethanol. (3) Salting out part of the histone, followed by removal of the nucleic acid from the remainder as an insoluble lanthanum salt. Similar yields were obtained in all cases. Measurements of fractional solubility, electrophoretic mobility, and sedimentation rate disclosed the presence of two principal components corresponding roughly to those obtained in the two stages of method (3). Alterations of one component were found to take place in aqueous solutions more alkaline than pH 4.0, resulting in the appearance of artifacts some of which sedimented more rapidly, while others sedimented more slowly, than the original material.

CONDUCTANCES OF CONCENTRATED AQUEOUS SOLUTIONS OF MIXED ELECTROLYTES

1958 ◽  
Vol 36 (10) ◽  
pp. 1325-1331 ◽  
Author(s):  
A. N. Campbell ◽  
E. M. Kartzmark ◽  
A. G. Sherwood
Keyword(s):  
Aqueous Solutions ◽  
Silver Nitrate ◽  
Ammonium Nitrate ◽  
Ion Concentration ◽  
Lithium Nitrate ◽  
Mixed Electrolytes ◽  
Salt Solutions ◽  
Concentrated Aqueous Solutions ◽  
The Mean

Equivalent conductances, viscosities, and densities were determined for solutions equimolar in two of the three salts lithium nitrate, ammonium nitrate, and silver nitrate. The three possible combinations of two salts were each studied at 25 °C and at 35 °C.The observed conductances and viscosities were compared with those of the single salt solutions at the same total ion concentration. The conductances were lower than the mean of the conductances of the single salt solutions. The viscosities were also lower than the mean viscosities.

scholarly journals EXPERIMENTAL OBSTRUCTION OF THE JEJUNUM

1925 ◽  
Vol 41 (6) ◽  
pp. 707-718 ◽  
Author(s):  
Russell L. Haden ◽  
Thomas G. Orr
Keyword(s):  
Sodium Chloride ◽  
Hydrochloric Acid ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Distilled Water ◽  
Salt Solutions ◽  
Chemical Changes ◽  
Sodium Chloride Solutions ◽  
Long Period ◽  
Cent Sodium

Chemical changes are reported occurring in the blood of animals with obstruction of the jejunum, in which distilled water or sodium chloride solutions were introduced directly into the lumen of the intestine below the point of obstruction. Distilled water given daily from the beginning of the obstruction, had no influence on the development or course of the toxemia. 1 and 2 per cent salt solutions prevented a toxemia in uncomplicated cases. One animal so treated lived 30 days. Distilled water, given after the onset of toxemia, did not alter the progress or outcome of the toxemia. 10 per cent sodium chloride solution, administered after the onset of toxemia, controlled it in most cases for a long period. Hydrochloric acid had no effect on the course of the toxemia.

PROPERTIES OF CALF THYMUS HISTONE ISOLATED BY THREE DIFFERENT METHODS

1958 ◽  
Vol 36 (1) ◽  
pp. 1-13 ◽  
Author(s):  
L. B. Smillie ◽  
G. C. Butler ◽  
David B. Smith
Keyword(s):  
Nucleic Acid ◽  
Hydrochloric Acid ◽  
Aqueous Solutions ◽  
Sedimentation Rate ◽  
Principal Components ◽  
Dilute Hydrochloric Acid ◽  
Original Material ◽  
Salting Out ◽  
Calf Thymus ◽  
Two Stages

Histone of calf thymus deoxyribonucleoprotein was isolated by three different methods: (1) Extraction from nucleoprotein solutions with dilute hydrochloric acid. (2) Extraction from cold saline nucleoprotein solutions with ethanol. (3) Salting out part of the histone, followed by removal of the nucleic acid from the remainder as an insoluble lanthanum salt. Similar yields were obtained in all cases. Measurements of fractional solubility, electrophoretic mobility, and sedimentation rate disclosed the presence of two principal components corresponding roughly to those obtained in the two stages of method (3). Alterations of one component were found to take place in aqueous solutions more alkaline than pH 4.0, resulting in the appearance of artifacts some of which sedimented more rapidly, while others sedimented more slowly, than the original material.

scholarly journals THE BINDING OF SODIUM CHLORIDE AND CALF THYMUS DESOXYPENTOSE NUCLEIC ACID

1952 ◽  
Vol 198 (1) ◽  
pp. 85-92
Author(s):  
Joseph. Shack ◽  
Robert J. Jenkins ◽  
Jacqueline M. Thompsett
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Calf Thymus

Specificity of sodium chloride aversion of hypertensive rats

1959 ◽  
Vol 196 (6) ◽  
pp. 1326-1332 ◽  
Author(s):  
Melvin J. Fregly
Keyword(s):  
Sodium Chloride ◽  
Salt Solution ◽  
Distilled Water ◽  
Nacl Solution ◽  
Salt Solutions ◽  
Hypertensive Rats ◽  
Threshold Levels ◽  
Preference Threshold

Rats made hypertensive by encapsulation of both kidneys with latex envelopes manifest a relative NaCl aversion if given choice between water and .15 m NaCl solution to drink. The specificity of this aversion was tested by offering hypertensive rats choice between salt solutions, other than NaCl, and water. It was observed that hypertensive rats manifested aversions for KCl, Na2SO4, LiCl and Na saccharin which were similar in character to the NaCl aversion. As soon as hypertensive rats were able to differentiate between any of these salt solutions and distilled water offered simultaneously, they rejected the salt solutions in favor of water. These animals never ingested more of any salt solution used than water. With the exception of the Na saccharin solutions, normal rats ingested more salt solution than water and rejected salt solution only at concentrations 3–10 times above preference threshold levels. Hence, it would appear that the NaCl aversion manifested by hypertensive rats is not specific, but part of a general salt aversion.

Notizen: Stability of the Liquid State of Concentrated Aqueous Solutions of Lithium Salts at Low Temperatures

1992 ◽  
Vol 47 (3) ◽  
pp. 543-544
Author(s):  
Tooru Hasebe ◽  
Kazuko Tanaka ◽  
Reita Tamamushi ◽  
Rie Sato
Keyword(s):  
Glass Transition ◽  
Aqueous Solutions ◽  
Liquid State ◽  
Low Temperatures ◽  
Lithium Salt ◽  
Lithium Salts ◽  
Salt Solutions ◽  
Concentrated Aqueous Solutions ◽  
Transition Points ◽  
The Stability

AbstractThe stability of the liquid state of aqueous lithium salt solutions was studied with a modified DTA technique. Aqueous solutions of LiCl and LiSCN in the concentration range 8-14 mol kg-1 , LiBr in the range 8 - 10 mol kg-1 maintain their liquid state for at least 50 hours at temperatures slightly above their glass transition points

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