PROPERTIES OF CALF THYMUS HISTONE ISOLATED BY THREE DIFFERENT METHODS

1958 ◽  
Vol 36 (1) ◽  
pp. 1-13 ◽  
Author(s):  
L. B. Smillie ◽  
G. C. Butler ◽  
David B. Smith
Keyword(s):  
Nucleic Acid ◽  
Hydrochloric Acid ◽  
Aqueous Solutions ◽  
Sedimentation Rate ◽  
Principal Components ◽  
Dilute Hydrochloric Acid ◽  
Original Material ◽  
Salting Out ◽  
Calf Thymus ◽  
Two Stages

Histone of calf thymus deoxyribonucleoprotein was isolated by three different methods: (1) Extraction from nucleoprotein solutions with dilute hydrochloric acid. (2) Extraction from cold saline nucleoprotein solutions with ethanol. (3) Salting out part of the histone, followed by removal of the nucleic acid from the remainder as an insoluble lanthanum salt. Similar yields were obtained in all cases. Measurements of fractional solubility, electrophoretic mobility, and sedimentation rate disclosed the presence of two principal components corresponding roughly to those obtained in the two stages of method (3). Alterations of one component were found to take place in aqueous solutions more alkaline than pH 4.0, resulting in the appearance of artifacts some of which sedimented more rapidly, while others sedimented more slowly, than the original material.

PROPERTIES OF CALF THYMUS HISTONE ISOLATED BY THREE DIFFERENT METHODS

1958 ◽  
Vol 36 (1) ◽  
pp. 1-13 ◽  
Author(s):  
L. B. Smillie ◽  
G. C. Butler ◽  
David B. Smith
Keyword(s):  
Nucleic Acid ◽  
Hydrochloric Acid ◽  
Aqueous Solutions ◽  
Sedimentation Rate ◽  
Principal Components ◽  
Dilute Hydrochloric Acid ◽  
Original Material ◽  
Salting Out ◽  
Calf Thymus ◽  
Two Stages

Histone of calf thymus deoxyribonucleoprotein was isolated by three different methods: (1) Extraction from nucleoprotein solutions with dilute hydrochloric acid. (2) Extraction from cold saline nucleoprotein solutions with ethanol. (3) Salting out part of the histone, followed by removal of the nucleic acid from the remainder as an insoluble lanthanum salt. Similar yields were obtained in all cases. Measurements of fractional solubility, electrophoretic mobility, and sedimentation rate disclosed the presence of two principal components corresponding roughly to those obtained in the two stages of method (3). Alterations of one component were found to take place in aqueous solutions more alkaline than pH 4.0, resulting in the appearance of artifacts some of which sedimented more rapidly, while others sedimented more slowly, than the original material.

Salts of Sulfated Oleic Acid Amides as Acid Corrosion Inhibitors

2021 ◽  
Vol 625 (3) ◽  
pp. 36-38
Author(s):  
E. K. Aminova ◽  
◽  
V. V. Fomina ◽  
Keyword(s):  
Oleic Acid ◽  
Sulfuric Acid ◽  
Hydrochloric Acid ◽  
Aqueous Solutions ◽  
Carboxylic Acids ◽  
Ammonium Salt ◽  
Dilute Hydrochloric Acid ◽  
Corrosion Inhibitors ◽  
Acid Corrosion ◽  
Protective Ability

This work is a continuation in a series of studies on the preparation of acid corrosion inhibitors based on carboxylic acids. A method of synthesis of acid corrosion inhibitors based on oleic acid amides has been developed. Several syntheses have been carried out to increase its inhibitory properties. The substances were obtained in several stages. At the first stage, amino alcohols were added to the ОA, then the resulting compounds were sulfonated with sulfuric acid. To expand the field of various inhibitors, reactions with bases are produced. As a result, salts of sulfated amides of oleic acid synthesized with amino nitrates, sulfuric acid and aqueous solutions of bases were formed. To establish the effectiveness of the compounds obtained, the protective ability in dilute hydrochloric acid was evaluated. It is established that some of the obtained substances exhibit the corresponding properties of inhibitors. In this case, the most effective is the ammonium salt of sulfated diethanolamide oleic acid.

THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (1) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (3) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

Kinetics of cyclization of S-ethoxycarbonylmethylisothiouronium chloride to 2-imino-4-thiazolidone

1979 ◽  
Vol 44 (3) ◽  
pp. 912-917 ◽  
Author(s):  
Vladimír Macháček ◽  
Said A. El-bahai ◽  
Vojeslav Štěrba
Keyword(s):  
Hydrochloric Acid ◽  
Dilute Hydrochloric Acid ◽  
Base Catalysis ◽  
General Base ◽  
Rate Limiting Step ◽  
Limiting Step ◽  
Kinetics Of Formation ◽  
Acid Catalyzed ◽  
Rate Limiting ◽  
Kinetics Of

Kinetics of formation of 2-imino-4-thiazolidone from S-ethoxycarbonylmethylisothiouronium chloride has been studied in aqueous buffers and dilute hydrochloric acid. The reaction is subject to general base catalysis, the β value being 0.65. Its rate limiting step consists in acid-catalyzed splitting off of ethoxide ion from dipolar tetrahedral intermediate. At pH < 2 formation of this intermediate becomes rate-limiting; rate constant of its formation is 2 . 104 s-1.

Synthesis of the semi-rigid analogues of prothiadene and dithiadene as potential antidepressant and antihistamine agents: 11-[2-(Dimethylaminomethyl)cyclohexylidene]-6,11-dihydrodibenzo[b,e]thiepins and 4-[2-(dimethylaminomethyl)cyclohexylidene]-4,9-dihydrothieno-[2,3-c]-2-benzothiepins

1985 ◽  
Vol 50 (5) ◽  
pp. 1078-1088 ◽  
Author(s):  
Zdeněk Polívka ◽  
Jiří Holubek ◽  
Emil Svátek ◽  
Jan Metyš ◽  
Miroslav Protiva
Keyword(s):  
Hydrochloric Acid ◽  
Ir Spectrum ◽  
Title Compound ◽  
Dilute Hydrochloric Acid ◽  
Amino Alcohols ◽  
Geometric Isomers ◽  
Pharmacological Testing

Reaction of dibenzo[b,e]thiepin-11(6H)-one with 2-(dimethylaminomethyl)cyclohexylmagnesium chloride gave a mixture of stereoisomeric amino alcohols IX from which four homogeneous bases (IXa to IXd) were separated by chromatography. Dehydration of these compounds with boiling dilute hydrochloric acid afforded mixtures of racemic geometric isomers of the title compound VII, which were separated by crystallization. To the prevailing less polar base VIIa (E)-configuration was assigned on the basis of the IR spectrum. Using a similar procedure, thieno[2,3-c]-2-benzothiepin-4(9H)-one gave mixture of amino alcohols X from which three homogeneous stereoisomers X-A to X-C were isolated. Their dehydration resulted in both expected racemic geometric isomers VIII-A and VIII-B. Pharmacological testing proved the character of an antidepressant for the semi-rigid analogue of dithiadene VIII.

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