THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

W. P. McKinley; W. A. Maw; W. F. Oliver; R. H. Common

doi:10.1139/y54-022

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y54-022 ◽

1954 ◽

Vol 32 (1) ◽

pp. 189-199 ◽

Cited By ~ 5

Author(s):

W. P. McKinley ◽

W. A. Maw ◽

W. F. Oliver ◽

R. H. Common

Keyword(s):

Serum Protein ◽

Filter Paper ◽

Domestic Fowl ◽

Serum Proteins ◽

Paper Electrophoresis ◽

Protein Fractions ◽

Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

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THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o54-022 ◽

1954 ◽

Vol 32 (3) ◽

pp. 189-199 ◽

Cited By ~ 4

Author(s):

W. P. McKinley ◽

W. A. Maw ◽

W. F. Oliver ◽

R. H. Common

Keyword(s):

Serum Protein ◽

Filter Paper ◽

Domestic Fowl ◽

Serum Proteins ◽

Paper Electrophoresis ◽

Protein Fractions ◽

Veronal Buffer

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Determination of Transaminase Activity of Serum Protein Fractions separated by Paper Electrophoresis

Nature ◽

10.1038/181915b0 ◽

1958 ◽

Vol 181 (4613) ◽

pp. 915-916 ◽

Cited By ~ 9

Author(s):

MIROSLAV ŠEVELA

Keyword(s):

Serum Protein ◽

Paper Electrophoresis ◽

Protein Fractions ◽

Transaminase Activity

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Filter Paper Electrophoresis of Serum Proteins of the Domestic Fowl.

Experimental Biology and Medicine ◽

10.3181/00379727-84-20642 ◽

1953 ◽

Vol 84 (2) ◽

pp. 346-351 ◽

Cited By ~ 23

Author(s):

W. P. McKinley ◽

W. F. Oliver ◽

W. A. Maw ◽

R. H. Common

Keyword(s):

Filter Paper ◽

Domestic Fowl ◽

Serum Proteins ◽

Paper Electrophoresis

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CHILDHOOD TUBERCULOSIS: CLINICAL VALUE OF THE ELECTROPHORETIC PATTERN OF THE SERUM PROTEINS

PEDIATRICS ◽

10.1542/peds.27.1.54 ◽

1961 ◽

Vol 27 (1) ◽

pp. 54-67

Author(s):

Rosa Lee Nemir ◽

Charlotte Marker Zitrin ◽

Paraskevi Tsouros ◽

Enriqueta Melly

Keyword(s):

Serum Protein ◽

Tuberculous Meningitis ◽

Gamma Globulin ◽

Serum Proteins ◽

Electrophoretic Pattern ◽

Miliary Tuberculosis ◽

Protein Fractions ◽

Reciprocal Relation ◽

Tuberculous Infection ◽

Tuberculous Disease

The blood serum protein fractions of 138 children with tuberculosis were analyzed by paper electrophoresis serially over a period of many months. Many manifestations of tuberculous infection were studied. The group was divided into 11 categories ranging from healed or arrested tuberculous disease to various stages of activity. The serum protein fractions were evaluated in terms of prognosis, type of tuberculous disease, effect of intercurrent infection and age of patient. It was found that the greatest changes occurred in the gamma-globulin and albumin fractions in reciprocal relation. With the exception of tuberculous meningitis, the increase in gamma-globulin usually corresponded to the severity of disease. Albumin was correspondingly decreased, and was low even in tuberculous meningitis. Both fractions approached normal levels as the patients improved. Relatively normal readings were found in patients with tuberculosis observation or arrested tuberculosis. The greatest deviation from normal was seen in patients with miliary tuberculosis and those with pleurisy with effusion. Here, the gamma and alpha2-globulins were very high and the serum albumin was low. The alpha2 fraction was elevated in the children with more severe disease, including tuberculous meningitis; with clinical improvement it returned to normal more rapidly than the gamma. A rise in the beta-globulin fraction suggests caseation. Confirmatory evidence was obtained in patients with endobronchial disease, tuberculous adenitis and from the only necropsy in the series. The significant changes in the various fractions are further described and discussed.

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THE BINDING OF THYROIDAL HORMONES BY TRITON WR-1339

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y63-204 ◽

1963 ◽

Vol 41 (1) ◽

pp. 1803-1809

Author(s):

William V. C. Leahy ◽

Thomas F. McNickle

Keyword(s):

Guinea Pig ◽

Serum Protein ◽

Filter Paper ◽

Serum Proteins ◽

Direct Method ◽

Triton Wr 1339

The ability of Triton WR-1339 to bind thyroxine was determined by the thyroxine-stabilization technic of Tata. In addition, the effect of Triton treatment on the amount of triiodothyronine bound by guinea pig plasma was measured by the erythrocytic system of Hamolsky et al. and the direct method of Scholer. It was found that Triton was as effective as whole serum protein in its ability to inhibit the drying-induced deiodination of thyroxine occurring on filter paper. Triton treatment resulted in a significantly decreased uptake of triiodothyronine in the erythrocytic system and, conversely, a significantly increased binding of triiodothyronine by plasma. These results are discussed in terms of a possible competition for available thyroxine in vivo between Triton and thyroxine-binding serum proteins.

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Serum Albumin and Other Serum Protein Fractions in Stable Patients on Peritoneal Dialysis

Peritoneal Dialysis International ◽

10.1177/089686080002000621 ◽

2000 ◽

Vol 20 (6) ◽

pp. 703-707 ◽

Cited By ~ 8

Author(s):

Francisco Caravaca ◽

Manuel Arrobas ◽

Carmen Dominguez

Keyword(s):

Peritoneal Dialysis ◽

Serum Albumin ◽

Serum Protein ◽

Serum Proteins ◽

Electrophoretic Pattern ◽

Residual Renal Function ◽

Protein Fractions ◽

Protein Loss ◽

Protein Nitrogen ◽

Baseline Values

Background Hypoalbuminemia is common in peritoneal dialysis (PD) patients; but the reduction in serum albumin levels (SAlb) that should be expected in stable PD patients is less clear. Objectives To determine prospectively, in a group of stable PD patients without comorbid conditions, the changes in SAlb concentration and in the concentrations of the other serum protein fractions. To investigate the best determinants of a significant decrease in SAlb levels. Design Prospective observational study. Methods Seventeen PD patients in stable clinical condition, with no signs of systemic inflammatory response, were included in the study. SAlb and the electrophoretic pattern of serum proteins were determined immediately before PD start, and after 6, 9, 12, 15, 18, 21, and 24 months on PD. In each study period, clinical characteristics, adequacy parameters, protein catabolic rate (PNPNA: protein equivalent of non protein nitrogen appearance), and protein losses were determined. Patients were divided into two subgroups according to whether SAlb decreased less than 10%, or 10% or more, from baseline values after 24 months on PD. The main differences between the subgroups were investigated. Results Mean SAlb did not decrease significantly after 24 months on PD (from baseline 3.99 ± 0.46 g/dL to 3.80 ± 0.54 g/dL), though percentage SAlb values did (58.36% ± 5.58% vs 55.15% ± 5.42%, p < 0.01). A weak increase in α2-globulin was observed after 18 months on PD (from 10.62% ± 2.53% to 12.96% ± 2.51%, p = 0.001). α1-Globulin showed a sustained increase from a mean baseline value of 3.51% ± 1.09% to 6.83% ± 2.13% after 24 months ( p < 0.0001). Seven patients had a reduction in SAlb greater than 10% after 24 months on PD. Kt/V urea and residual renal function tended to be lower in patients whose SAlb decreased. Mean PNPNA was significantly lower in patients who had a reduction in SAlb (0.76 ± 0.12 g/kg/day vs 0.96 ± 0.12 g/kg/day, p < 0.0001). However, total protein loss was even greater in patients who had no SAlb reduction. Conclusions After 24 months on PD, a mean reduction in SAlb of 10% – 15% from baseline values should be expected only in those stable patients whose PNPNA is low.

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