THE BINDING OF THYROIDAL HORMONES BY TRITON WR-1339

1963 ◽  
Vol 41 (1) ◽  
pp. 1803-1809
Author(s):  
William V. C. Leahy ◽  
Thomas F. McNickle
Keyword(s):  
Guinea Pig ◽  
Serum Protein ◽  
Filter Paper ◽  
Serum Proteins ◽  
Direct Method ◽  
Triton Wr 1339

The ability of Triton WR-1339 to bind thyroxine was determined by the thyroxine-stabilization technic of Tata. In addition, the effect of Triton treatment on the amount of triiodothyronine bound by guinea pig plasma was measured by the erythrocytic system of Hamolsky et al. and the direct method of Scholer. It was found that Triton was as effective as whole serum protein in its ability to inhibit the drying-induced deiodination of thyroxine occurring on filter paper. Triton treatment resulted in a significantly decreased uptake of triiodothyronine in the erythrocytic system and, conversely, a significantly increased binding of triiodothyronine by plasma. These results are discussed in terms of a possible competition for available thyroxine in vivo between Triton and thyroxine-binding serum proteins.

THE BINDING OF THYROIDAL HORMONES BY TRITON WR-1339

1963 ◽  
Vol 41 (8) ◽  
pp. 1803-1809
Author(s):  
William V. C. Leahy ◽  
Thomas F. McNickle
Keyword(s):  
Guinea Pig ◽  
Serum Protein ◽  
Filter Paper ◽  
Serum Proteins ◽  
Direct Method ◽  
Triton Wr 1339

The ability of Triton WR-1339 to bind thyroxine was determined by the thyroxine-stabilization technic of Tata. In addition, the effect of Triton treatment on the amount of triiodothyronine bound by guinea pig plasma was measured by the erythrocytic system of Hamolsky et al. and the direct method of Scholer. It was found that Triton was as effective as whole serum protein in its ability to inhibit the drying-induced deiodination of thyroxine occurring on filter paper. Triton treatment resulted in a significantly decreased uptake of triiodothyronine in the erythrocytic system and, conversely, a significantly increased binding of triiodothyronine by plasma. These results are discussed in terms of a possible competition for available thyroxine in vivo between Triton and thyroxine-binding serum proteins.

scholarly journals EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

1963 ◽  
Vol 118 (1) ◽  
pp. 99-120 ◽  
Author(s):  
J. D. Broome
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Serum Proteins ◽  
Deae Cellulose ◽  
Salt Precipitation ◽  
Asparaginase Activity ◽  
Pig Serum

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.

scholarly journals STUDIES ON THE EFFECT OF TRITON (WR-1339) ON GUINEA PIG TISSUES

1958 ◽  
Vol 107 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Paul C. Hudgins ◽  
Robert A. Patnode
Keyword(s):  
Guinea Pig ◽  
Thermal Shock ◽  
Whole Blood ◽  
Single Injection ◽  
Experimental Tuberculosis ◽  
Paraffin Oil ◽  
Triton Wr 1339 ◽  
Increased Sensitivity

Guinea pig red cells exposed to triton WR-1339 either in vitro or in vivo demonstrate an increased sensitivity to hemolysis by thermal shock. Although the sensitivity of guinea pig leucocytes to thermal shock remains unchanged after a single injection of triton, their resistance to disruption by acetone and by sonic vibration is increased significantly. Resistance to sonic vibration appears to be maximum within a few hours after injection and then it gradually wanes. Paraffin oil-induced peritoneal exudate cells (monocytes) and leucocytes from whole blood are rendered more resistant to disruption by sonic vibration when exposed to triton in vitro. These findings are discussed briefly in relation to the therapeutic effect of triton in experimental tuberculosis.

scholarly journals KINETICS OF AMINO ACID INCORPORATION INTO SERUM PROTEINS

1955 ◽  
Vol 38 (3) ◽  
pp. 283-293 ◽  
Author(s):  
H. Green ◽  
H. S. Anker
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Serum Protein ◽  
Transit Time ◽  
Serum Proteins ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Amino Acid Analogues ◽  
Kinetics Of

1. The effect of varying body temperature on the rate of amino acid incorporation into serum protein does not give support to the idea that the rate of this process is adjusted in vivo to restore those protein molecules destroyed by thermal denaturation. The experimentally observed Q10 was about 3.9. 2. When amino acids are injected into the blood of animals in a steady state of serum protein turnover, a period of time elapses before these amino acids can be found in the serum proteins. This has been called transit time. At a given temperature (31°) it is the same in rabbits, turtles, and Limulus (1 hour). In rabbits and turtles it has a Q10 of 3.2. It appears to be specifically related to the process of synthesis (or release) of serum proteins. 3. It was not possible to affect the transit time or the incorporation rate by the administration of amino acid analogues.

scholarly journals STUDIES ON THE EFFECT OF TRITON (WR-1339) ON GUINEA PIG TISSUES

1958 ◽  
Vol 107 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Robert A. Patnode ◽  
Paul C. Hudgins
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Concentration Level ◽  
Intradermal Injection ◽  
Skin Sensitivity ◽  
Lytic Effect ◽  
Triton Wr 1339

The administration of triton WR-1339 (300 mg./kg. subcutaneously) to tuberculin-sensitive guinea pigs 2 hours before the intradermal injection of PPD depresses slightly their skin sensitivity to tuberculin and essentially obliterates the lytic effect of tuberculin on their circulating leucocytes. When leucocytes from tuberculin-sensitive guinea pigs are exposed to triton in vitro at a concentration level attainable in vivo the cells are partially protected against lysis by PPD.

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (1) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

IODINATION OF SERUM PROTEINS IN VIVO: RELATIONSHIP TO IRRADIATION AND HYPERTROPHY OF THE THYROID

1969 ◽  
Vol 44 (4) ◽  
pp. 467-NP ◽  
Author(s):  
E. TRIANTAPHYLLIDIS ◽  
B. D. THOMPSON ◽  
C. F. BARNABY ◽  
B. JASANI
Keyword(s):  
Thyroid Carcinoma ◽  
Serum Protein ◽  
Radioactive Iodine ◽  
Serum Proteins ◽  
Protein Labelling ◽  
Low Levels

SUMMARY High levels of labelled serum protein were found in rats with hypertrophied and colloid-depleted thyroids, but only after high thyroid irradiating doses of 131I. Low levels were found in rats with normal thyroids after low or high thyroid irradiating-doses of radioactive iodine. The levels in rats with hypertrophied thyroids after low thyroid-irradiating doses of 125I did not differ greatly from those in rats with normal thyroids. The mechanism of serum protein labelling is discussed and it is suggested that similar conditions may be important for the production of high levels of labelled serum protein in patients treated with radioactive iodine for functioning thyroid carcinoma.

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (3) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

In vivo activity of pyrimidine-dispirotripiperaziniumin in the male guinea pig model of genital herpes

2020 ◽  
Vol 09 (01) ◽  
Author(s):  
Novoselova EA ◽  
Alimbarova LM ◽  
Monakhova NS ◽  
Lepioshkin AY ◽  
Ekins S ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Genital Herpes ◽  
Pig Model ◽  
Guinea Pig Model
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