Determination of Transaminase Activity of Serum Protein Fractions separated by Paper Electrophoresis

Nature ◽  
1958 ◽  
Vol 181 (4613) ◽  
pp. 915-916 ◽  
Author(s):  
MIROSLAV ŠEVELA
Keyword(s):  
Serum Protein ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Transaminase Activity

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (1) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (3) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Measurement of Polarographically Active Sulfur in Human Serum Fractions Obtained by Paper Electrophoresis

1970 ◽  
Vol 16 (3) ◽  
pp. 155-157 ◽  
Author(s):  
Jiří Homolka
Keyword(s):  
Human Serum ◽  
Serum Protein ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
X Ray ◽  
Serum Fractions

Abstract Polarographically active sulfur was distributed among the serum-protein fractions as follows (in percentages): albumin, 63.21; α1-globulin, 3.17; α2-globulin, 7.92; β-globulin, 14.54; α-globulin, 11.16. The results compare well with those obtained by X-ray spectrometry. The same method was applied to sera from patients suffering from malignant processes and some other diseases; the percentage of polarographically active sulfur in fractions of plasma from diseased individuals varied considerably.

scholarly journals Quality control in the determination of serum protein fractions

1969 ◽  
Vol 14 (2) ◽  
pp. 137-142
Author(s):  
Shinobu Tomita ◽  
Junko Nishikata ◽  
Toshiko Ueda
Keyword(s):  
Quality Control ◽  
Serum Protein ◽  
Protein Fractions
Sign in / Sign up

Export Citation Format

Share Document