Effects of nitrogen deficiency and recovery fertilization on ultrastructure, growth, and mineral concentrations of Scots pine needles

1995 ◽  
Vol 25 (2) ◽  
pp. 198-207 ◽  
Author(s):  
V. Palomâki ◽  
T. Holopainen
Keyword(s):  
Endoplasmic Reticulum ◽  
Scots Pine ◽  
Rapid Development ◽  
Nitrogen Concentration ◽  
Nitrogen Deficiency ◽  
Growing Season ◽  
Ultrastructural Level ◽  
First Year ◽  
Growing Period ◽  
Chloroplast Stroma

During the first year of a 2-year field experiment, 3-year-old Scots pine (Pinussylvestris L.) seedlings were exposed to one of three nitrogen fertilization treatments: no nitrogen, 30% optimal nitrogen, or optimal nitrogen. In the second growing period the experiment was continued as a recovery fertilization experiment. Nitrogen concentration of needles was clearly lower in seedlings of both deficiency levels than in the control seedlings by the end of the first growing season. There was a rapid development of external and ultrastructural symptoms in the first-year nitrogen-deficient seedlings. After 10 weeks of treatment, yellowing of needles, decline in growth, and reduction in ribosome and endoplasmic reticulum content at the ultrastructural level were observed compared with the controls. After 16 weeks of treatment, abnormally shaped and narrow chloroplasts were detected in the mesophyll and transfusion parenchyma tissues of current- and previous-year needles. Increased plastoglobuli number and sizes, decreased numbers of thylakoids, and decreasing electron density of chloroplast stroma followed the other changes. In the second growing season during the recovery fertilization phase, the color of the needles and the shape of the chloroplasts rapidly returned to normal, but the cytoplasmic degeneration did not totally recover. The results of the present experiment suggest that early degeneration of cytoplasmic endoplasmic reticulum and ribosomes and changes in the chloroplast shape can be regarded as characteristic symptoms of nitrogen deficiency.

Effects of phosphorus deficiency and recovery fertilization on growth, mineral concentration, and ultrastructure of Scots pine needles

1994 ◽  
Vol 24 (12) ◽  
pp. 2459-2468 ◽  
Author(s):  
V. Palomäki ◽  
T. Holopainen
Keyword(s):  
Scots Pine ◽  
Phosphorus Deficiency ◽  
Ultrastructural Level ◽  
First Year ◽  
Mineral Concentration ◽  
Growing Period ◽  
Recovery Treatment ◽  
Further Development ◽  
Complete Nutrient Solution ◽  
Nutrient Solutions

During the first year of a 2-year field experiment (1989–1990), 3-year-old Scots pine (Pinussylvestris L.) seedlings were watered with phosphorus-deficient nutrient solutions containing no phosphorus (0%) or 30% of the optimal phosphorus supply. A complete nutrient solution was used as the control. In the second growing period, the seedlings were divided into two groups, a deficiency and a recovery treatment, to follow the further development of, and recovery from, symptoms. Phosphorus contents in both current- and previous-year needles in both deficiency groups decreased clearly during the first growing period. Limited growth was observed at both deficiency levels after 10 weeks of treatment. The first ultrastructural symptom, swelling of cristae and subsequent dilatation of whole mitochondria, was observed after 16 weeks of treatment in the current-year needles. In addition to this symptom, the previous-year needles had an increase in the number and translucency of plastoglobuli, a decrease of granum thylakoids, and an increase in the density of the stroma in chloroplasts. After the second growing period, the same changes were observable in the phosphorus-deficient seedlings. In the young needles grown during the recovery fertilization period, slight swelling of mitochondria was detected and recovery of the previous-year needles was not complete. The results of this experiment suggest that the swelling of mitochondria is a characteristic symptom of phosphorus deficiency at the ultrastructural level in different needle generations. All the symptoms, when occurring together, may be used in identifying a moderate phosphorus-deficiency stress in seedlings.

scholarly journals Growth Responses of Boreal Scots Pine, Norway Spruce and Silver Birch Seedlings to Simulated Climate Warming over Three Growing Seasons in a Controlled Field Experiment

Forests ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 943
Author(s):  
Katri Nissinen ◽  
Virpi Virjamo ◽  
Antti Kilpeläinen ◽  
Veli-Pekka Ikonen ◽  
Laura Pikkarainen ◽  
...  
Keyword(s):  
Norway Spruce ◽  
Scots Pine ◽  
Root Biomass ◽  
Growing Season ◽  
Silver Birch ◽  
Shoot Biomass ◽  
Growth Responses ◽  
Growing Seasons ◽  
Simulated Climate ◽  
Shoot And Root Biomass

We studied the growth responses of boreal Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies L. Karst.) and silver birch (Betula pendula Roth) seedlings to simulated climate warming of an average of 1.3 °C over the growing season in a controlled field experiment in central Finland. We had six replicate plots for elevated and ambient temperature for each tree species. The warming treatment lasted for the conifers for three growing seasons and for the birch two growing seasons. We measured the height and diameter growth of all the seedlings weekly during the growing season. The shoot and root biomass and their ratios were measured annually in one-third of seedlings harvested from each plot in autumn. After two growing seasons, the height, diameter and shoot biomass were 45%, 19% and 41% larger in silver birch seedlings under the warming treatment, but the root biomass was clearly less affected. After three growing seasons, the height, diameter, shoot and root biomass were under a warming treatment 39, 47, 189 and 113% greater in Scots pine, but the root:shoot ratio 29% lower, respectively. The corresponding responses of Norway spruce to warming were clearly smaller (e.g., shoot biomass 46% higher under a warming treatment). As a comparison, the relative response of height growth in silver birch was after two growing seasons equal to that measured in Scots pine after three growing seasons. Based on our findings, especially silver birch seedlings, but also Scots pine seedlings benefitted from warming, which should be taken into account in forest regeneration in the future.

scholarly journals Soil Nitrogen Dynamics in a Managed Temperate Grassland Under Changed Climatic Conditions

Water ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 931
Author(s):  
Mona Giraud ◽  
Jannis Groh ◽  
Horst H. Gerke ◽  
Nicolas Brüggemann ◽  
Harry Vereecken ◽  
...  
Keyword(s):  
Nitrogen Uptake ◽  
Inorganic Nitrogen ◽  
Nitrogen Concentration ◽  
Nitrogen Dynamics ◽  
Climatic Conditions ◽  
Undisturbed Soil ◽  
Nitrogen Emissions ◽  
Growing Period ◽  
Grassland Type ◽  
Wide Range

Grasslands are one of the most common biomes in the world with a wide range of ecosystem services. Nevertheless, quantitative data on the change in nitrogen dynamics in extensively managed temperate grasslands caused by a shift from energy- to water-limited climatic conditions have not yet been reported. In this study, we experimentally studied this shift by translocating undisturbed soil monoliths from an energy-limited site (Rollesbroich) to a water-limited site (Selhausen). The soil monoliths were contained in weighable lysimeters and monitored for their water and nitrogen balance in the period between 2012 and 2018. At the water-limited site (Selhausen), annual plant nitrogen uptake decreased due to water stress compared to the energy-limited site (Rollesbroich), while nitrogen uptake was higher at the beginning of the growing period. Possibly because of this lower plant uptake, the lysimeters at the water-limited site showed an increased inorganic nitrogen concentration in the soil solution, indicating a higher net mineralization rate. The N2O gas emissions and nitrogen leaching remained low at both sites. Our findings suggest that in the short term, fertilizer should consequently be applied early in the growing period to increase nitrogen uptake and decrease nitrogen losses. Moreover, a shift from energy-limited to water-limited conditions will have a limited effect on gaseous nitrogen emissions and nitrate concentrations in the groundwater in the grassland type of this study because higher nitrogen concentrations are (over-) compensated by lower leaching rates.

scholarly journals Seed mass effects on germination and growth of diverse European Scots pine populations

1994 ◽  
Vol 24 (2) ◽  
pp. 306-320 ◽  
Author(s):  
P.B. Reich ◽  
J. Oleksyn ◽  
M.G. Tjoelker
Keyword(s):  
Growth Rate ◽  
Relative Growth Rate ◽  
Scots Pine ◽  
Field Experiments ◽  
Seed Mass ◽  
Growing Season ◽  
Turkish Population ◽  
Dry Mass ◽  
Relative Growth ◽  
Mass Effects

Seedlings of 24 European Scots pine (Pinussylvestris L.) populations were grown in controlled environment chambers under simulated photoperiodic conditions of 50 and 60°N latitude to evaluate the effect of seed mass on germination and seedling growth characteristics. Seeds of each population were classified into 1-mg mass classes, and the four classes per population with the highest frequencies were used. Photoperiod had minimal influence on seed mass effects. Overall, seed mass was positively related to the number of cotyledons and hypocotyl height. Populations differed significantly in seed mass effect on biomass. In northern populations (55–61°N), dry mass at the end of the first growing season was little affected by seed mass. However, dry mass in 9 of 15 central populations (54–48°N) and all southern (<45°N) populations correlated positively with seed mass. Relative growth rate was not related to seed mass within or across populations, and thus early growth is largely determined by seed mass. Relative growth rate also did not differ among populations, except for a geographically isolated Turkish population with the highest seed mass and lowest relative growth rate. After one growing season, height was positively correlated (r2 > 0.6) with seed mass in 15 populations. To check the duration of seed mass effects, height growth of 1- to 7-year-old field experiments established with the same seed lots were compared. Seed mass effects on height were strongest for 1-year-old seedlings and declined or disappeared by the age of 5–7 years among central and southern populations, but remained stable over that time in northern populations.

Early growth of lodgepole pine after establishment of alsike clover–Rhizobium nitrogen-fixing symbiosis

1992 ◽  
Vol 22 (8) ◽  
pp. 1089-1093 ◽  
Author(s):  
R. Trowbridge ◽  
F.B. Holl
Keyword(s):  
Forest Floor ◽  
Lodgepole Pine ◽  
Nitrogen Concentration ◽  
Growing Season ◽  
Early Growth ◽  
Percent Cover ◽  
Foliar Nitrogen ◽  
Growing Seasons ◽  
Pine Seedlings ◽  
Needle Mass

An overdense lodgepole pine (Pinuscontorta Dougl. ex Loud.) stand was knocked down and the site was prepared by broadcast burn, windrow burn, or mechanical forest floor removal. Inoculated alsike clover (Trifoliumhybridum L.) was seeded at 0, 10, 20, and 30 kg/ha for the three different site preparation treatments to determine the effects of (i) site preparation on infection and effectiveness of the clover–Rhizobium symbiosis and clover percent cover and (ii) the clover–Rhizobium N2-fixing symbiosis on survival, early growth, and foliar nitrogen concentration of lodgepole pine seedlings. The N2-fixing symbiosis established well in all treatments. Clover percent cover increased with increasing rate of seeding, although by relatively few percent in the clover seeded plots. Broadcast burning, windrow burning, and mechanical forest floor removal did not affect the establishment of the N2-fixing symbiosis or clover percent cover. Lodgepole pine survival was not affected by the seeding treatments in any year, nor were height measurements during the first three growing seasons. Seedling height was slightly less in clover-seeded plots compared with controls in the fourth growing season. Lodgepole pine seedlings on clover-seeded plots had decreased diameter growth compared with controls during the first three growing seasons, but incremental diameter growth no longer showed this effect by the fourth growing season. Needle mass (g/100 needles) was less in clover-seeded plots at the end of the second growing season, but this effect was reversed by the fourth growing season, when both needle mass and foliar nitrogen concentration in lodgepole pine foliage were greater in clover-seeded plots.

Structure of the chloroplast and its DNA in chloromonadophycean algae

1981 ◽  
Vol 49 (1) ◽  
pp. 401-409
Author(s):  
A.W. Coleman ◽  
P. Heywood
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Membrane ◽  
Nuclear Envelope ◽  
Chloroplast Dna ◽  
Single Layer ◽  
Longitudinal Axis ◽  
Chloroplast Envelope ◽  
Large Numbers ◽  
Gonyostomum Semen ◽  
Chloroplast Stroma

The arrangement and ultrastructure of chloroplasts is described for the Chloromonadophycean algae gonyostomum semen Diesing and Vacuolaria virescens Cienkowsky. The chloroplasts are present in large numbers and are discoid structures approximately 3–4 micrometer in length by 2–3 micrometer in width. In Gonyostomum semen the chloroplasts form a single layer immediately interior to the cell membrane; frequently their longitudinal axis parallels the longitudinal axis of the cell. The chloroplasts in Vacuolaria virescens are more than I layer deep and do not appear to be preferentially oriented. In both organisms, chloroplast bands usually consist of 3 apposed thylakoids, although fusion and interconnections between adjacent bands frequently occur. External to the girdle band (the outermost thylakoids) is the chloroplast envelope. This is bounded by endoplasmic reticulum but there is no immediately apparent continuity between this endoplasmic reticulum and the nuclear envelope. Electron-dense spheres in the chloroplast stroma are thought to be lipid food reserve. Ring-shaped electron-translucent regions in the chloroplast contain chloroplast DNA. The DNA is distributed along this ring in an uneven fashion and, when stained, resembles a string of beads. Each plastid has I ring, and the ring is unbroken in the intact plastid.

scholarly journals PRODUCTIVITY OF PERENNIAL GRASSES AND MIXTURES WITH DIFFERENT SOWING DATES IN WESTERN SIBERIA

2020 ◽  
pp. 24-32
Author(s):  
V. A. Petruk
Keyword(s):  
Dry Matter ◽  
Crop Yields ◽  
Growing Season ◽  
Single Species ◽  
Field Studies ◽  
Perennial Grasses ◽  
First Year ◽  
Sowing Dates ◽  
Second Year ◽  
Sowing Period

The results of field studies for 2017 - 2019 are presented. yields of perennial grasses sown at different times of the growing season. Spring, summer, and winter sowing periods were compared. Alfalfa, clover, rump, and also their mixtures were sown in 2017 under the cover of barley. The value of the cover crop yield of spring and summer sowing periods did not differ significantly and amounted to 4-5 t / ha of absolutely dry matter. Winter barley crops have not formed. On average, over 2 years of use, the highest yields were observed in alfalfa-crust grass mixtures - 3.4 t / ha of absolutely dry matter. The lowest yield was obtained in the single-species seeding of the rump. Correspondingly, in the spring, summer and winter periods of sowing, the yield of rump was 1.6; 1.1 and 1.3 t / ha. With a late sowing period, the yield of perennial grasses is significantly lower compared to spring and summer. With winter sowing periods, the yield was the highest for grass stands of alfalfa and alfalfacrust grass mixture - 2.3 and 2.4 t / ha. It should be noted that in the second year of use, the yield by the sowing dates in single-species crops and grass mixtures is leveled. The winter crops of perennial grasses in the first year of use formed a low yield. Only in the second year (third year of life) the productivity of perennial grasses of winter sowing began to increase. Consequently, in the area under perennial grasses of the winter sowing period, during one growing season (the next year after sowing), the crop was not actually formed. Based on the data obtained, production can be recommended for spring and summer planting of perennial grasses under the cover of barley. The winter sowing period provides economically valuable crop yields only by the third year of life.

Nitrate metabolism in Scots pine seedlings during their first growing season

1987 ◽  
Vol 3 (3) ◽  
pp. 285-293 ◽  
Author(s):  
T. Sarjala ◽  
R. Raitio ◽  
E.-M. Turkki
Keyword(s):  
Scots Pine ◽  
Growing Season ◽  
Pine Seedlings ◽  
Nitrate Metabolism

SEASONAL LEADER GROWTH OF LODGEPOLE PINE IN THE SUBALPINE FOREST OF ALBERTA

1958 ◽  
Vol 34 (4) ◽  
pp. 382-386 ◽  
Author(s):  
K. W. Horton
Keyword(s):  
Growth Rates ◽  
Direct Relationship ◽  
Lodgepole Pine ◽  
Growing Season ◽  
Subalpine Forest ◽  
Growing Period

The weekly leader growth of lodgepole pine saplings showed similar patterns but different growth rates according to aspect of site. The seasonal growing period consistently started in early May and was 12 weeks long but within this period the distribution of growth differed widely from year to year. A direct relationship existed between weekly growth and corresponding mean weekly temperatures, except toward the end of the growing season.

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