Identification and potential enzyme capacity of flavobacteria isolated from the rhizosphere of barley (Hordeum vulgare L.)

2009 ◽  
Vol 55 (3) ◽  
pp. 234-241 ◽  
Author(s):  
Jens Efsen Johansen ◽  
Preben Nielsen ◽  
Svend Jørgen Binnerup
Keyword(s):  
Hordeum Vulgare ◽  
16S Rdna ◽  
Gliding Motility ◽  
The Other ◽  
Plant Roots ◽  
Protein Digestion ◽  
Young Plant ◽  
Hordeum Vulgare L ◽  
Partial Sequencing

The diversity of 99 flavobacterium-like isolates from a barley rhizosphere is described. They were identified on 1/10 strength tryptic soy agar by their yellowish colour and a flexirubin reaction after exposure to 10% KOH.16S rDNA partial sequencing identified the majority (70%) of isolates as Flavobacterium . Twelve percent of the isolates belonged to other genera in the phylum Bacteroidetes. Finally 17% of the isolates did not belong to the phylum Bacteroidetes. Most of the Flavobacterium isolates were affiliated to various aquatic, validly named species and likely represent a bulk of undescribed soil Flavobacterium species found in especially high numbers in the rhizosphere of young plant roots. Most Flavobacterium isolates showed gliding motility on CY agar and VY/2 agar, whereas none of the other isolates shared this feature. A high percentage of Flavobacterium strains produced enzymes involved in polysaccharide and protein digestion as well as extracellular phosphatases, compared with strains related to other genera in the phylum Bacteroidetes.

COMPARISON OF SORGHUM WITH WHEAT AND BARLEY GROWN ON DRYLAND

1981 ◽  
Vol 61 (1) ◽  
pp. 37-43 ◽  
Author(s):  
D. J. MAJOR ◽  
W. M. HAMMAN
Keyword(s):  
Triticum Aestivum ◽  
Hordeum Vulgare ◽  
Slow Growth ◽  
Triticum Aestivum L ◽  
The Other ◽  
Hordeum Vulgare L ◽  
Whole Plant ◽  
Drought Conditions ◽  
Plant Yields ◽  
Sorghum Grain

Sorghum (Sorghum bicolor Moench ’Pride P130’), wheat (Triticum aestivum L. ’Neepawa’), and barley (Hordeum vulgare L. ’Galt’) were harvested at 1-wk intervals on dryland at Lethbridge, Alberta, in 1976 and 1977 and separated into leaves, stems, heads, and seed. Whole-plant yields were higher in 1976 than in 1977 and sorghum whole-plant yields were higher than barley or wheat in both years. Sorghum grain yields were similar to those of barley, but were greater than those of wheat. Although sorghum is about 40 days later maturing than barley or wheat, its whole-plant and filling-period durations were not very different from the other two crops. Sorghum used water more efficiently than wheat or barley under drought conditions. The biggest disadvantage of present sorghum hybrids appears to be slow growth in spring.

Agronomic response to seeding rate of two- and six-rowed barley cultivars in central Alberta

1995 ◽  
Vol 75 (2) ◽  
pp. 315-320 ◽  
Author(s):  
P. E. Jedel ◽  
J. H. Helm
Keyword(s):  
Hordeum Vulgare ◽  
Yield Components ◽  
The Other ◽  
Seeding Rate ◽  
Kernel Number ◽  
Hordeum Vulgare L ◽  
Grain Yields ◽  
Barley Cultivars ◽  
Kernel Weights ◽  
Yield Responses

Little information is available on the response of two-rowed barley (Hordeum vulgare L.) cultivars to seeding rate in central Alberta. The objective of this research was to compare the effects of seeding rates of 129–344 seeds m−2 (50–140 kg ha−1) on the agronomic response of two- and six-rowed barley grown in central Alberta. Grain yields of 5.8–6.1 t ha−1 were not affected by seeding rates in 1990 and 1991 at Lacombe. As seeding rates increased in 1992, grain yields at Lacombe increased from 6.4 to 7.8 t ha−1; at Olds, from 3.5 to 4.0 t ha−1; and at Botha for the 129 seeds m−2 treatment, yields were only 5.5 t ha−1, compared with 5.8 to 6.0 t ha−1 for the other treatments. Effects of seeding rate on test and kernel weights varied between location–years. Tiller numbers m−2 were found to increase at higher seeding rates for the two-rowed cultivars, Abee and Harrington, while for the six-rowed cultivars, Noble and Virden, these numbers remained constant. Node numbers spike−1 decreased with higher seeding rates for both two- and six-rowed cultivars. There was little effect of seeding rate on kernel number spike−1 for the two-rowed cultivars, while for the six-rowed cultivars this number decreased. Although the two- and six-rowed cultivars differed in their partitioning of yield and the effects of seeding rates on these components, their yield responses to seeding rate did not differ. Recommended seeding rates for barley in central Alberta should be above 129 seeds m−2 (50 kg ha−1). To maintain high kernel and test weights, seeding rates for barley grown in central Alberta for malt quality should range from 172 to 258 seeds m−2. When conditions warrant accelerated development, seeding rates of 300 or more seeds m−2 should be used. Key words:Hordeum vulgare, management, yield components

Effects of environment and cultivar on total mixed-linkage β-glucan content in eastern and western Canadian barleys (Hordeum vulgare L.)

1995 ◽  
Vol 75 (2) ◽  
pp. 371-376 ◽  
Author(s):  
P. Narasimhalu ◽  
D. Kong ◽  
T. M. Choo ◽  
K. M. Ho ◽  
T. Ferguson ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Key Words ◽  
The Other ◽  
Food Type ◽  
Western Canada ◽  
Hordeum Vulgare L ◽  
Key Words Barley ◽  
The Mean

Two-row and six-row barley (Hordeum vulgare L.) cultivars, originating from eastern and western Canada were grown in six environments across Canada. The grain was studied for the effects of environment and cultivar on the total mixed-linkage β-glucan content (TBG, g hg−1 DM). Environment significantly affected TBG content in barley. TBG content differed among the 32 eastern or 43 western Canadian cultivars but the mean TBG for the eastern cultivars was not different from the mean of western cultivars. All eastern cultivars were hulled-feed types, and their TBG ranged from 3.31 in Micmac to 4.09 in Birka for two-row barleys, and from 3.78 in OAC Kippen to 4.41 in Maskot for six-row barleys. Western hulled cultivars which included feed and malt types, contained TBG ranging from 3.37 in Manley to 4.14 in Betzes for two-row barleys, and from 3.71 in Heartland to 4.42 in Tankard for six-row barleys. The two-row hulless cultivar, Condor, contained more TBG (4.69) than the other two hulless cultivars, CDC Richard (3.91) and Scout (3.85). The six-row hulless cultivars, CDC Buck and Tupper, were equal in TBG (4.35). The mean TBG was lower for the two- than six-row barleys (3.7 vs. 4.0), and a broader range for TBG in the two- (3.31–4.55) than six-row barleys (3.71–4.42) suggesed the greater possibility of producing low and high TBG barleys from the two-row cultivars. Canadian cultivars contained between 3.31 and 4.55 g TBG hg−1 DM, and there is a need to develop feed-type barleys containing a third less than the minimum TBG, and food-type barleys containing a third more than the maximum TBG. Key words: Barley, Hordeum vulgare cultivars, β-glucan

DEVELOPMENT OF CYTOLYTIC ENZYME ACTIVITIES DURING GERMINATION OF DIFFERENT BARLEYS

1974 ◽  
Vol 54 (2) ◽  
pp. 223-229 ◽  
Author(s):  
G. M. BALLANCE ◽  
W. O. S. MEREDITH
Keyword(s):  
Hordeum Vulgare ◽  
Gibberellic Acid ◽  
Enzyme Activities ◽  
Hydrolase Activity ◽  
The Other ◽  
Hordeum Vulgare L ◽  
Glucanase Activity

The development of three cytolytic enzyme activities was assessed at sequential stages of germination for water-steeped and also gibberellic acid-treated samples of six barley (Hordeum vulgare L.) cultivars. The cytolytic enzymes were barley-β-glucan endo-hydrolase, endo-β 1,4-glucanase and endo-β 1,3-glucanase. Significant differences in the activity development profiles of the three enzymes were noted. Differences between cultivars were quite apparent for the barley-β-glucan endo-hydrolase activities but not for the other two activities. Gibberellic acid enhanced the barley-β-glucan endo-hydrolase activity within cultivars to varying degrees, slightly increased endo-β 1,3-glucanase activity and had no effect on endo-β 1,4-glucanase activities.

scholarly journals Influence of potassium on growth, content of mineral nutrients and yield formation of the spring barley (Hordeum vulgare L.)

2006 ◽  
Vol 54 (1) ◽  
pp. 145-154
Author(s):  
Josef Zehnálek ◽  
Vojtěch Adam ◽  
René Kizek
Keyword(s):  
Hordeum Vulgare ◽  
Model Experiment ◽  
Spring Barley ◽  
Dry Weight ◽  
Mineral Nutrients ◽  
The Other ◽  
Hordeum Vulgare L ◽  
Lower Growth ◽  
Yield Increase ◽  
Yield Formation

In model experiment, an influence of increasing KCl doses on spring barley growth, accumulation of main mineral nutrients (N, P, K, Ca, Mg and Na) in main stalks, offshoots and roots and on yield formation and its structure was observed. Increase of weight of above-ground parts of plants was inhibited only at the beginning of experiment by increasing KCl doses, particularly, significantly lower growth of offshoots, but on the other hand production of dry weight of roots was negatively influenced during whole cultivation. Accumulation of main mineral nutrients in the plants was mostly influenced at potassium. Uptake of potassium by plant increased up to 37%. Yield of kernels was higher at variants with application of KCl but the correlation between higher K doses and the yield increase has not been statistically proved. Increase in the yield was reached by higher number of offshoots, higher number of kernels and higher weight of kernels in comparison with control. Content of main mineral nutrients in kernel was not influenced by application of KCl, but potassium content in straw was significantly increased.

Effects of picloram on growth and on chlorophyll, RNA, and protein content of plants

1972 ◽  
Vol 50 (10) ◽  
pp. 2039-2044 ◽  
Author(s):  
M. P. Sharma ◽  
W. H. Vanden Born
Keyword(s):  
Glycine Max ◽  
Hordeum Vulgare ◽  
Protein Content ◽  
Ribonucleic Acid ◽  
Foliar Application ◽  
The Other ◽  
Cirsium Arvense ◽  
Canada Thistle ◽  
Hordeum Vulgare L ◽  
Glycine Max L

Foliar application of picloram (4-amino-3,5,6-trichloropicolinic acid) markedly inhibited the growth of soybean (Glycine max (L.) Merr., cv. Harosoy 63) and Canada thistle (Cirsium arvense (L.) Scop.). The rates of picloram which caused death of soybean (0.14 kg/hectare) and Canada thistle (0.56 kg/hectare) in 2 weeks caused no marked injury symptoms to barley (Hordeum vulgare L. cv. Parkland). ED50 values of picloram (doses required to reduce growth by half) were 16.1 g/hectare for soybean, 18.2 g/hectare for Canada thistle, and 3.64 kg/hectare for barley.Picloram (250 mg/liter, about 0.30 kg/hectare), both 1 and 3 days after treatment, markedly reduced the chlorophyll content of soybean and Canada thistle plants. RNA (ribonucleic acid) and protein contents of such picloram-treated plants, on the other hand, were increased up to 30% over controls. In barley, picloram had very little effect on chlorophyll, RNA, and protein content.

THE EFFECT OF LOCATION ON NATURAL SELECTION IN BULK POPULATIONS OF BARLEY (HORDEUM VULGARE L.). I. SIMPLY INHERITED TRAITS

1980 ◽  
Vol 60 (1) ◽  
pp. 31-40 ◽  
Author(s):  
T. M. CHOO ◽  
H. R. KLINCK ◽  
C. A. ST-PIERRE
Keyword(s):  
Natural Selection ◽  
Hordeum Vulgare ◽  
The Other ◽  
Hordeum Vulgare L ◽  
Rates Of Change ◽  
Single Cross ◽  
Minor Influence ◽  
A Minor ◽  
Almost All

The effect of location on natural selection was investigated by studying the changes in phenotypic composition of six simply inherited traits. Seventeen F15 unselected bulk populations were derived from a single cross of barley. Fifteen of the bulks were alternated between Macdonald College and La Pocatière either annually or after 2–8 yr at one location; one of the remaining two bulks was grown continuously at Macdonald College and the other at La Pocatière. By F15, almost all populations had shifted toward plants with rough awns, yellow aleurone, short-haired rachillas, hairy rachis, or a dense spike. The V-shaped collar type predominated in these populations. The propagation environments had a significant effect on the rates of change in phenotypic composition of the six simply inherited traits in these barley bulk populations. However, the frequency of alternating populations between the two locations appeared to have only a minor influence on the rates of change in phenotypic composition of these traits.

Ultrastructure and Senescence in an Achloroplastic Mutant of Hordeum vulgare L. cv. Dyan

1992 ◽  
Vol 50 (1) ◽  
pp. 844-845
Author(s):  
R.H.M. Cross ◽  
C.E.J. Botha ◽  
A.K. Cowan ◽  
B.J. Hartley
Keyword(s):  
Cell Wall ◽  
Hordeum Vulgare ◽  
Leaf Tissue ◽  
Ultrastructural Changes ◽  
Hordeum Vulgare L ◽  
Mesophyll Cells ◽  
Lethal Mutant ◽  
Cytoplasmic Matrix ◽  
Close Proximity ◽  
Pinocytotic Vesicles

Senescence is an ordered degenerative process leading to death of individual cells, organs and organisms. The detection of a conditional lethal mutant (achloroplastic) of Hordeum vulgare has enabled us to investigate ultrastructural changes occurring in leaf tissue during foliar senescence.Examination of the tonoplast structure in six and 14 day-old mutant tissue revealed a progressive degeneration and disappearance of the membrane, apparently starting by day six in the vicinity of the mitochondria associated with the degenerating proplastid (Fig. 1.) where neither of the plastid membrane leaflets is evident (arrows, Fig. 1.). At this stage there was evidence that the mitochondrial membranes were undergoing retrogressive changes, coupled with disorganization of cristae (Fig. 2.). Proplastids (P) lack definitive prolamellar bodies. The cytoplasmic matrix is largely agranular, with few endoplasmic reticulum (ER) cisternae or polyribosomal aggregates. Interestingly, large numbers of actively-budding dictysomes, associated with pinocytotic vesicles, were observed in close proximity to the plasmalemma of mesophyll cells (Fig. 3.). By day 14 however, mesophyll cells showed almost complete breakdown of subcellular organelle structure (Fig. 4.), and further evidence for the breakdown of the tonoplast. The final stage of senescence is characterized by the solubilization of the cell wall due to expression and activity of polygalacturonase and/or cellulose. The presence of dictyosomes with associated pinocytotic vesicles formed from the mature face, in close proximity to both the plasmalemma and the cell wall, would appear to support the model proposed by Christopherson for the secretion of cellulase. This pathway of synthesis is typical for secretory glycoproteins.

ИЗМЕНЧИВОСТЬ ЯЧМЕНЯ (Hordeum vulgare L.) РАЗНОГО ГЕОГРАФИЧЕСКОГО ПРОИСХОЖДЕНИЯ ПО ЭЛЕМЕНТАМ СТРУКТУРЫ УРОЖАЯ

2012 ◽  
pp. 33-40
Author(s):  
А.В. ЖЕЛЕЗНОВ ◽  
◽  
Н.Б. ЖЕЛЕЗНОВА ◽  
Т.В. КУКОЕВА ◽  
Н.В. БУРМАКИНА ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Hordeum Vulgare L
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