BIOCHEMICAL EFFECTS OF IRRADIATION ON LIVER REGENERATION

1960 ◽  
Vol 38 (10) ◽  
pp. 1059-1068 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Deoxyribonucleic Acid ◽  
Normal Liver ◽  
Cell Nuclei ◽  
Local Irradiation ◽  
Biochemical Effects ◽  
Marked Accumulation ◽  
Liver Region ◽  
Rat Livers ◽  
Weight Number ◽  
Effects Of Irradiation On

Little change in the total weight, number of cell nuclei, and amounts of protein, ribonucleic acid, and deoxyribonucleic acid occurs in rat livers after local irradiation of the liver region. However, irradiation of the normal liver before partial hepatectomy does retard subsequent DNA accumulation and increase in number of cell nuclei. These effects appear to be due to a localized action of the radiation on the liver cells. The lag in DNA accumulation is not accompanied by a marked accumulation of acid-soluble purine–deoxyribose derivatives.

BIOCHEMICAL EFFECTS OF IRRADIATION ON LIVER REGENERATION

1960 ◽  
Vol 38 (1) ◽  
pp. 1059-1068 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Deoxyribonucleic Acid ◽  
Normal Liver ◽  
Cell Nuclei ◽  
Local Irradiation ◽  
Biochemical Effects ◽  
Marked Accumulation ◽  
Liver Region ◽  
Rat Livers ◽  
Weight Number ◽  
Effects Of Irradiation On

Little change in the total weight, number of cell nuclei, and amounts of protein, ribonucleic acid, and deoxyribonucleic acid occurs in rat livers after local irradiation of the liver region. However, irradiation of the normal liver before partial hepatectomy does retard subsequent DNA accumulation and increase in number of cell nuclei. These effects appear to be due to a localized action of the radiation on the liver cells. The lag in DNA accumulation is not accompanied by a marked accumulation of acid-soluble purine–deoxyribose derivatives.

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

Analytical studies on the shoot apex of Helianthus annuus

1969 ◽  
Vol 47 (9) ◽  
pp. 1367-1375 ◽  
Author(s):  
T. A. Steeves ◽  
M. Anne Hicks ◽  
J. M. Naylor ◽  
Patricia Rennie
Keyword(s):  
Helianthus Annuus ◽  
Shoot Apex ◽  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Vegetative State ◽  
Central Zone ◽  
Apical Region ◽  
Cell Nuclei ◽  
Liquid Culture Medium ◽  
Vegetative Shoot Apex

The vegetative shoot apex of Helianthus annuus contains a central zone in which the cell nuclei are relatively large and stain faintly in the Feulgen reaction. Excised apices in the vegetative state were supplied with thymidine-H3 through their sterile, liquid culture medium. Autoradiography after 24 or 48 hours of feeding revealed no significant incorporation of the labeled precursor into central zone nuclei, but extensive incorporation in peripheral regions of the apex. It is concluded that during vegetative growth deoxyribonucleic acid (DNA) synthesis and mitosis are arrested in the central zone or reduced to an extremely slow rate. Microspectrophotometry, however, indicates that the central zone nuclei are not held at the 2C level. With the onset of flowering, cytological zonation disappears in the apex and the incorporation of thymidine-H3 is uniformly heavy throughout the apical region.

Effects of Irradiation on Synthesis of Deoxyribonucleic Acid and Mitosis in Synchronous Cultures of Hela Cells

Nature ◽  
1966 ◽  
Vol 210 (5034) ◽  
pp. 393-395 ◽  
Author(s):  
T. P. BRENT ◽  
J. A. V. BUTLER ◽  
A. R. CRATHORN
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Synchronous Cultures ◽  
Effects Of Irradiation On
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