Distribution of nucleic acids in the cell nuclei of normal liver and transplanted mouse hepatoma

1958 ◽  
Vol 45 (1) ◽  
pp. 45-47
Author(s):  
S. M. Saidov
Keyword(s):  
Nucleic Acids ◽  
Normal Liver ◽  
Cell Nuclei ◽  
Mouse Hepatoma

BIOCHEMICAL EFFECTS OF IRRADIATION ON LIVER REGENERATION

1960 ◽  
Vol 38 (10) ◽  
pp. 1059-1068 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Deoxyribonucleic Acid ◽  
Normal Liver ◽  
Cell Nuclei ◽  
Local Irradiation ◽  
Biochemical Effects ◽  
Marked Accumulation ◽  
Liver Region ◽  
Rat Livers ◽  
Weight Number ◽  
Effects Of Irradiation On

Little change in the total weight, number of cell nuclei, and amounts of protein, ribonucleic acid, and deoxyribonucleic acid occurs in rat livers after local irradiation of the liver region. However, irradiation of the normal liver before partial hepatectomy does retard subsequent DNA accumulation and increase in number of cell nuclei. These effects appear to be due to a localized action of the radiation on the liver cells. The lag in DNA accumulation is not accompanied by a marked accumulation of acid-soluble purine–deoxyribose derivatives.

scholarly journals The Use of Fractal Features from the Periphery of Cell Nuclei as a Classification Tool

1999 ◽  
Vol 19 (1) ◽  
pp. 21-37 ◽  
Author(s):  
Birgitte Nielsen ◽  
Fritz Albregtsen ◽  
Håvard E. Danielsen
Keyword(s):  
Normal Liver ◽  
Analysis Tool ◽  
Cell Nuclei ◽  
Bhattacharyya Distance ◽  
Prior Probabilities ◽  
Hepatocellular Carcinomas ◽  
Transmission Electron ◽  
Mouse Liver Cell ◽  
A Cell ◽  
Classification Tool

A polygonization‐based method is used to estimate the fractal dimension and several new scalar lacunarity features from digitized transmission electron micrographs (TEM) of mouse liver cell nuclei. The fractal features have been estimated in different segments of 1D curves obtained by scanning the 2D cell nuclei in a spiral‐like fashion called “peel‐off scanning”. This is a venue to separate estimates of fractal features in the center and periphery of a cell nucleus. Our aim was to see if a small set of fractal features could discriminate between samples from normal liver, hyperplastic nodules and hepatocellular carcinomas. The Bhattacharyya distance was used to evaluate the features. Bayesian classification with pooled covariance matrix and equal prior probabilities was used as the rule for classification. Several single fractal features estimated from the periphery of the cell nuclei discriminated samples from the hyperplastic nodules and hepatocellular carcinomas from normal ones. The outer 25–30% of the cell nuclei contained important texture information about the differences between the classes. The polygonization‐based method was also used as an analysis tool to relate the differences between the classes to differences in the chromatin structure.

The ribosenucleic acid content of isolated cell nuclei

1952 ◽  
Vol 214 (1118) ◽  
pp. 427-427
Keyword(s):  
Nucleic Acids ◽  
Acid Content ◽  
Isolation Procedure ◽  
Cell Nuclei ◽  
Isolated Nuclei ◽  
Rna Content ◽  
Cytoplasmic Rna ◽  
Different Types ◽  
Isolated Cell

A method is described for the estimation, by furfural formation, of small amounts of ribosenucleic acid (RNA) in the presence of large amounts of deoxyribosenucleic acid. Evidence is produced to show that the Schmidt and Thannhauser method does not give a satisfactory separation of the two types of nucleic acids. The method has been applied to the estimation of the RNA content of nuclei isolated from a number of different types of cell, and the values reported. It is concluded that the nuclei as isolated contain small amounts of RNA, but the possibility of adsorption of cytoplasmic RNA by the nuclei during the isolation procedure cannot be excluded. No correlation was found between the RNA content of the isolated nuclei and the prominence of their nucleolar systems.

BIOCHEMICAL EFFECTS OF IRRADIATION ON LIVER REGENERATION

1960 ◽  
Vol 38 (1) ◽  
pp. 1059-1068 ◽  
Author(s):  
D. K. Myers
Keyword(s):  
Deoxyribonucleic Acid ◽  
Normal Liver ◽  
Cell Nuclei ◽  
Local Irradiation ◽  
Biochemical Effects ◽  
Marked Accumulation ◽  
Liver Region ◽  
Rat Livers ◽  
Weight Number ◽  
Effects Of Irradiation On

Little change in the total weight, number of cell nuclei, and amounts of protein, ribonucleic acid, and deoxyribonucleic acid occurs in rat livers after local irradiation of the liver region. However, irradiation of the normal liver before partial hepatectomy does retard subsequent DNA accumulation and increase in number of cell nuclei. These effects appear to be due to a localized action of the radiation on the liver cells. The lag in DNA accumulation is not accompanied by a marked accumulation of acid-soluble purine–deoxyribose derivatives.

THE EFFECTS OF CORTISOL ON THE INCORPORATION OF GLYCINE CARBON INTO THE NUCLEIC ACIDS OF NORMAL AND MALIGNANT TISSUES

1964 ◽  
Vol 47 (1) ◽  
pp. 85-94
Author(s):  
W. de Loecker
Keyword(s):  
Skeletal Muscle ◽  
Nucleic Acids ◽  
Acid Metabolism ◽  
Normal Liver ◽  
The Other ◽  
Nucleic Acid Metabolism ◽  
Adrenal Steroids ◽  
Rna And Dna ◽  
Different Tissues ◽  
Hepatoma Tissue

ABSTRACT Normal and hepatoma bearing rats were treated with cortisol in order to study the effect of adrenal steroids on nucleic acid metabolism of different tissues. Normal liver, precancerous liver, hepatoma nodules and skeletal muscle were used in these experiments. The incorporation of 14C from glycine-2-14C into RNA and DNA fractions of different tissues was examined. The incorporation of glycine carbon into RNA of normal and precancerous liver was found to be stimulated by cortisol treatment. The RNA fraction of hepatoma tissue of non treated animals showed a higher incorporation level than that observed in liver, but incorporation was drastically inhibited by cortisol. The DNA fraction of hepatoma tissue showed a higher incorporation than the DNA fraction from the other tissues examined, and followed the same incorporation pattern as RNA. The incorporation of 14C into the nucleic acids of skeletal muscle was not affected by cortisol treatment.

Multifunctional magnetic and fluorescent core–shell nanoparticles for bioimaging

Nanoscale ◽  
2015 ◽  
Vol 7 (5) ◽  
pp. 1606-1609 ◽  
Author(s):  
Yanjiao Lu ◽  
Bicheng He ◽  
Jie Shen ◽  
Jie Li ◽  
Wantai Yang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Strong Interaction ◽  
Near Infrared ◽  
Core Shell ◽  
Cell Nuclei ◽  
Shell Nanoparticles ◽  
Near Infrared Fluorescence ◽  
Superparamagnetic Behavior ◽  
Core Shell Nanoparticles

Magnetic and fluorescent core–shell nanoparticles exhibit superparamagnetic behavior and emit strong near-infrared fluorescence. The nanoparticles are highly biocompatible and accumulate in cell nuclei via strong interaction with nucleic acids.

The ribosenucleic acid content of isolated cell nuclei

1952 ◽  
Vol 140 (898) ◽  
pp. 18-31 ◽  
Keyword(s):  
Nucleic Acids ◽  
Acid Content ◽  
Isolation Procedure ◽  
Cell Nuclei ◽  
Isolated Nuclei ◽  
Rna Content ◽  
Cytoplasmic Rna ◽  
Different Types ◽  
Isolated Cell

A method is described for the estimation, by furfural formation, of small amounts of ribosenucleic acid (RNA) in the presence of large amounts of deoxyribosenucleic acid. Evidence is produced to show that the Schmidt & Thannhauser method does not give a satisfactory separation of the two types of nucleic acids. The method has been applied to the estimation of the RNA content of nuclei isolated from a number of different types of cell, and the values reported. It is concluded that the nuclei as isolated contain small amounts of RNA, but the possibility of adsorption of cytoplasmic RNA by the nuclei during the isolation procedure cannot be excluded. No correlation was found between the RNA content of the isolated nuclei and the prominence of their nucleolar systems.

Production of serum proteins in normal diploid fibroblast-hepatoma cell hybrids and in A9-normal liver cell hybrids

1979 ◽  
Vol 35 (1) ◽  
pp. 267-279
Author(s):  
J. Szpirer ◽  
C. Szpirer
Keyword(s):  
Liver Cell ◽  
Serum Proteins ◽  
Hepatoma Cell ◽  
Normal Liver ◽  
Liver Cells ◽  
Complement C3 ◽  
Rat Serum ◽  
Normal Liver Cell ◽  
Cell Hybrids ◽  
Mouse Hepatoma

Two series of interspecific hybrids have been generated between liver cells (which actively secrete several serum proteins) and fibroblasts (which do not). In each series, one of the parental cells was a normal diploid cell: mouse hepatoma cells were fused with normal diploid rat fibroblasts, and normal rat liver cells were fused with mouse fibroblasts of the permanent line A9. The production of albumin, alpha-fetoprotein (AFP) transferrin and the third component of complement (C3) was analysed in these hybrids. Most hepatoma cell hybrids exhibit extinction of albumin, AFP and (to a lesser extent) transferrin; they retain the capacity to secrete C3. Normal liver cell hybrids are also characterized by the absence of albumin and transferrin production and by retention of C3 secretion. These results, when compared to previous results obtained with hybrids derived exclusively from different differentiated cells of permanent and transformed lines show that the phenotype of such hybrids is not determined by the abnormal character per se of the aneuploid parental cells. Amongst the rat fibroblast-mouse hepatoma cell hybrids, a few clones retain the capacity to actively secrete mouse albumin, AFP and transferrin, without the concomitant production of the rat serum proteins. These hybrids have lost more rat (fibroblast) chromosomes than the other clones and also have an increased number of mouse (hepatoma) chromosomes. Thus, their phenotype must result from either the complete loss of ‘extinguisher’ chromosomes, or gene dosage effects. The significance of the lack of rat serum protein production is also discussed, and it is suggested that retention, without concomitant activation, could be explained in terms of diffusible regulators and heritable differences in chromatin conformation.

The Use of Spermidine for the Isolation of Nuclei from Mouse Liver. Studies of Purity and Yield During Different Physiological Conditions

1974 ◽  
Vol 29 (5-6) ◽  
pp. 267-271 ◽  
Author(s):  
Eric Christensson ◽  
Lillemor Lewan
Keyword(s):  
Partial Hepatectomy ◽  
Nuclear Membrane ◽  
Liver Cell ◽  
Mouse Liver ◽  
Normal Liver ◽  
Rnase Activity ◽  
Cell Nuclei ◽  
Normal Liver Cell ◽  
Physiological Conditions ◽  
Bivalent Cations

A 15 min method for the isolation of clean liver cell nuclei from normal, starved, hepatectomized and sham operated mice in mediums not containing bivalent cations is described. The iso­lation medium contained 0.5 mм spermidine for stabilization of the nuclear membrane, and macaloid and EDTA for inhibition of RNase activity. The RNA/DNA ratio in normal liver cell nuclei was 0.25 - 0.29 and the protein/DNA ratio 5.2. The RNA/DNA ratio was 0.23-0.25 after starvation for 24 hours and increased to 0.5 at 72 and 144 hours after partial hepatectomy.

scholarly journals DNA Measurement of Overlapping Cell Nuclei in Thick Tissue Sections

1997 ◽  
Vol 14 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Liang Ji ◽  
James Tucker
Keyword(s):  
Liver Tissue ◽  
Nuclear Dna ◽  
Selection Procedure ◽  
Normal Liver ◽  
Cell Nuclei ◽  
3D Segmentation ◽  
Isolated Nuclei ◽  
Tissue Sections ◽  
The Individual ◽  
Dna Measurements

The paper describes an improved image analysis procedure for measuring the DNA content of cell nuclei in thick sections of liver tissue by absorption densitometry. Whereas previous methods only permitted the analysis of isolated nuclei, the new technique enables both isolated and overlapping nuclei to be measured. A 3D segmentation procedure determines whether each object is an isolated nucleus or a pair of overlapping nuclei; in the latter case the combined optical density is redistributed to the individual nuclei. A selection procedure ensures that only complete nuclei are measured.The method has been tested on specially‐prepared Feulgen‐stained 20μsections of normal liver tissue. The overall distribution of the nuclear DNA measurements shows well‐defined diploid and tetraploid peaks, with coefficient of variations of less than 10%. Similar distributions were obtained from both the isolated nuclei and overlapped nuclei sub‐populations.

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