Isolation and identification of rumen bacteria capable of anaerobic phloroglucinol degradation

1975 ◽  
Vol 21 (6) ◽  
pp. 794-801 ◽  
Author(s):  
Chii-Guary Tsai ◽  
G. A. Jones
Keyword(s):  
Rumen Fluid ◽  
Brain Heart Infusion ◽  
Rumen Bacteria ◽  
Streptococcus Bovis ◽  
Anaerobic Conditions ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Fluid Medium ◽  
De Man ◽  
Gram Positive Cocci

Eight strains of rumen bacteria capable of degrading phloroglucinol (1,3,5-trihydroxybenzene) under anaerobic conditions were isolated from enrichment cultures of the bovine rumen microflora established in a prereduced medium containing 0.02 M phloroglucinol. Five of the strains were facultatively anaerobic Gram-positive streptococci which were identified as Streptococcus bovis. Three strains of obligately anaerobic Gram-positive cocci were assigned to the genus Coprococcus. Anaerobic cultures of the Streptococcus bovis strains in a 40% rumen fluid medium initially containing 0.02 M phloroglucinol degraded 50–80% of the substrate within 2 days, whereas cultures of the Coprococcus strains degraded more than 80% of the substrate under the same conditions. The Streptococcus bovis strains were incapable of degrading phloroglucinol in brain heart infusion or in the medium of de Man, Rogosa, and Sharpe (MRS broth) incubated aerobically.

Isolation and identification of rumen bacteria capable of anaerobic rutin degradation

1969 ◽  
Vol 15 (12) ◽  
pp. 1365-1371 ◽  
Author(s):  
K. -J. Cheng ◽  
G. A. Jones ◽  
F. J. Simpson ◽  
M. P. Bryant
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Anaerobic Bacteria ◽  
Heterocyclic Ring ◽  
Ring Structure ◽  
Water Soluble ◽  
Rumen Bacteria ◽  
Isolation And Identification ◽  
Pure Cultures ◽  
Rutin Degradation

Fifteen strains of bacteria capable of degrading rutin anaerobically were isolated from bovine rumen contents and identified by morphological and biochemical evidence as strains of Butyrivibrio sp. Three cultures from a laboratory collection of 53 strains of rumen bacteria also used rutin anaerobically. Two, Butyrivibrio fibrisolvens D1 and Selenomonas ruminantium GA192, cleaved the glycosidic bond of rutin and fermented the sugar but did not degrade the insoluble aglycone produced; the third strain, Peptostreptococcus sp. B178, degraded the substrate to soluble products. Butyrivibrio sp. C3 degraded rutin, quercitrin, and naringin to water-soluble products, showing that the organism cleaved the heterocyclic ring of these compounds. Butyrivibrio sp. C3 fermented the sugar moiety of hesperidin but did not cleave the heterocyclic ring. It did not attack quercetin, taxifolin, protocatechuic acid, or phloroglucinol. In a medium containing rumen fluid, Butyrivibrio sp. C3 degraded rutin more than twice as fast as it did in a medium containing enzymatic casein hydrolyzate, volatile fatty acids, yeast extract, and hemin in place of rumen fluid.The observations reported in this paper are believed to represent the first recorded demonstration of degradation of the heterocyclic ring structure of rutin and other bioflavonoids in pure cultures of anaerobic bacteria.

Products of anaerobic phloroglucinol degradation by Coprococcus sp. Pe15

1976 ◽  
Vol 22 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Chii-Guary Tsai ◽  
Diane M. Gates ◽  
W. M. Ingledew ◽  
G. A. Jones
Keyword(s):  
Carbon Dioxide ◽  
Acetic Acid ◽  
Rumen Fluid ◽  
Cell Suspensions ◽  
Anaerobic Conditions ◽  
Fluid Medium ◽  
Resting Cell ◽  
Flavonoid Compounds

Under anaerobic conditions, resting cell suspensions of Coprococcus sp. Pe15 degraded 1 molecule of phloroglucinol to 2 molecules of acetic acid and 2 molecules of carbon dioxide. The organism metabolized the flavonoids rhamnetin and quercetin anaerobically in 20% rumen fluid medium but failed to grow under similar conditions at the expense of any of 39 other aromatic or flavonoid compounds tested.

Antibiotic activity of an isocyanide metabolite of Trichoderma hamatum against rumen bacteria

1985 ◽  
Vol 31 (9) ◽  
pp. 767-772 ◽  
Author(s):  
S. N. Liss ◽  
D. Brewer ◽  
A. Taylor ◽  
G. A. Jones
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Rumen Fluid ◽  
Cellulose Hydrolysis ◽  
Soluble Carbohydrate ◽  
Rumen Bacteria ◽  
Fermentation Products ◽  
Fluid Medium ◽  
Trichoderma Hamatum ◽  
Significant Depression

A metabolite of Trichoderma hamatum, 3-(3-isocyanocyclopent-2-enylidene)propionic acid, was tested for its effects on growth of and carbohydrate metabolism in 11 strains of functionally important rumen bacteria. To standardize the biological activity of this unstable metabolite, a rapid, aerobic disc diffusion assay was developed using Escherichia coli ATCC 11775. In an anaerobic broth dilution assay using a medium lacking rumen fluid and containing a soluble carbohydrate, the minimum inhibitory concentration of the metabolite which completely inhibited growth of the rumen bacteria for 18 h at 39 °C was generally < 10 μg∙mL−1; however, the minimum inhibitory concentrations for Megasphaera elsdenii B159 and Streptococcus bovis Pe18 were 10–25 and 25–64 μg∙mL−1, respectively. In general, the Gram-negative strains were more sensitive than the Gram positive. The minimum inhibitory concentration for Bacteroides ruminicola 23 grown with glucose was 1 μg∙mL−1; for B. ruminicola GA33 (glucose), B. succinogenes S85 (cellobiose), and Succinivibrio dextrinosolvens 24 (maltose), it was 2 μg∙mL−1. When added to a cellulose-containing rumen fluid medium, 1–4 μg∙mL−1 of the metabolite delayed cellulose hydrolysis by B. succinogenes S85, Ruminococcus albus 7, and R. flavefaciens FD1 for up to 4 days, and 6–7 μg∙mL−1 prevented hydrolysis for at least 1 month. In the presence of the metabolite, the proportion of acetate produced from soluble carbohydrate by the majority of strains increased, but with some strains net production of acetate decreased relative to production of other acidic fermentation products. If the metabolite gained entrance to the rumen, a concentration of as little as 1 μg∙mL−1 would probably cause a significant depression of the fermentation and result in nutritional deprivation of the animal.

Human Skin Flora as a Potential Source of Epidural Abscess

1996 ◽  
Vol 85 (6) ◽  
pp. 1276-1282. ◽  
Author(s):  
Sukeyuki Sato ◽  
Tadakazu Sakuragi ◽  
Kenjiro Dan
Keyword(s):  
Staphylococcus Epidermidis ◽  
Brain Heart Infusion ◽  
Epidural Block ◽  
Sweat Glands ◽  
Gram Positive ◽  
Potential Source ◽  
Skin Disinfection ◽  
Skin Flora ◽  
Back Surgery ◽  
Gram Positive Cocci

Background The mechanism of epidural infection associated with epidural block is not clearly understood. Resident organisms in skin specimens were studied after skin was prepared with disinfectants. Methods Sixty-nine paired skin specimens were excised at incisional sites after skin disinfection with 10% povidone-iodine (10% PVP-I) or 0.5% chlorhexidine in 80% ethanol (0.5% CHE) from 60 patients having back surgery. One of the specimen pairs was placed in 10 ml brain-heart infusion broth and incubated in air at 37 degrees C for 96 h. The other specimen was sectioned at 3 microns and prepared with Gram's stain for examination with the microscope. Results Thirteen gram-positive staphylococcal species (Staphylococcus epidermidis, 69.2%; S. hyicus, 15.4%; and S. capitis, 15.4%) were isolated from cultures. The isolates were found in a significantly greater proportion of the skin specimens disinfected with 10% PVP-I than in those disinfected with 0.5% CHE (11 of 34 cultures [32.4%] vs. 2 of 35 cultures [5.7%]; P &lt; 0.01). Many gram-positive cocci were observed with the microscope in 4 (11.8%) and 5 (14.3%) of 34 and 35 skin specimens disinfected with 10% PVP-I and 0.5% CHE, respectively. The cocci formed a dense colony in each follicle and in the stratum corneum. No organism was present in any of 17,584 sweat glands examined. Conclusions In a large proportion of patients, isolation of viable organisms from excised skin specimens after disinfection with 10% PVP-I suggests that contamination of the epidural space by the skin flora may be a potential mechanism of epidural infection associated with epidural block.

scholarly journals Digestion of rumen bacteria in vitro

1983 ◽  
Vol 49 (1) ◽  
pp. 101-108 ◽  
Author(s):  
R. J. Wallace
Keyword(s):  
Rumen Fluid ◽  
Individual Species ◽  
Rumen Bacteria ◽  
Gram Negative Bacteria ◽  
Bacterial Protein ◽  
Gram Positive ◽  
Gram Negative ◽  
Pure Cultures ◽  
Mixed Bacteria

1. A pepsin + pancreatin method was used to assess the digestibility of pure cultures of rumen bacteria and mixed bacteria prepared from rumen fluid.2. Individual species of Gram-negative rumen bacteria were highly digestible, whereas Gram-positive species, especially cocci, were more resistant to digestion.3. A similar difference was observed microscopically with mixed rumen bacteria, but the influence of the relative proportions of Gram-positive and Gram-negative bacteria on the digestibility of bacterial protein in rumen fluid was small.

scholarly journals Tyrosine degradation in presumptive identification of Peptostreptococcus anaerobius

1979 ◽  
Vol 9 (3) ◽  
pp. 358-361
Author(s):  
J B Babcock
Keyword(s):  
Clinical Laboratory ◽  
Agar Plate ◽  
Anaerobic Conditions ◽  
Gram Positive ◽  
Single Strain ◽  
Clinical Specimens ◽  
Multiple Cultures ◽  
Presumptive Identification ◽  
Reference Strains ◽  
Gram Positive Cocci

A new tyrosine medium was developed and evaluated for the differentiation of Peptostreptococcus anaerobius from other anaerobic, gram-positive cocci. The strains included 159 originating from clinical specimens and 13 reference strains received from other workers in the field. Only one strain of each species was included in the study from multiple cultures from the same patient. This medium is simple to prepare and can be used in a small clinical laboratory. One hundred seventy-two strains of anaerobic gram-positive cocci were grown and evaluated with the new tyrosine medium; 36 strains (100%) of P. anaerobius degradated the tyrosine crystals when incubated at 37 degrees C under anaerobic conditions in a GasPak jar (Baltimore Biological Laboratory) for approximately 72 h. On the other hand, 135 of 136 other anaerobic gram-positive cocci were negative for tyrosine degradation, but grew on the tyrosine agar plate when incubated anaerobically. The single strain that degraded tyrosine was 1 of the 13 isolates of Peptostreptococcus micros studied.

Frothy feedlot bloat in cattle: production of extracellular polysaccharides and development of viscosity in cultures of Streptococcus bovis

1976 ◽  
Vol 22 (4) ◽  
pp. 450-459 ◽  
Author(s):  
K.-J. Cheng ◽  
R. Hironaka ◽  
G. A. Jones ◽  
Thalia Nicas ◽  
J. W. Costerton
Keyword(s):  
Rumen Fluid ◽  
Synthetic Medium ◽  
Uranyl Acetate ◽  
Ruthenium Red ◽  
Extracellular Polysaccharides ◽  
Foam Formation ◽  
Streptococcus Bovis ◽  
Fluid Medium ◽  
Freeze Etching ◽  
The Media

Streptococcus bovis was cultured in a synthetic medium with three concentrations of sucrose. Initial viscosity of the media was 1.5 centipoise (cp). After incubation for 8 h, the viscosity of the medium with 0.5% sucrose was unchanged, that with 3% sucrose had increased to 8 cp, and that with 6% sucrose to 112 cp. Similar results were found with a rumen fluid medium. A slimy material, responsible for increased viscosity of these cultures, was digested by dextranase. The material appeared as a complex system of intercellular fibers when viewed under the electron microscope after freeze-etching. With proteins and other polymers released from lysed bacteria, this slimy material may contribute directly to increased viscosity and foam formation. In addition to these intercellular fibers, each cell was surrounded by a fibrous capsule that was not digested by dextranase. This capsule stained with lead citrate and uranyl acetate, but not with ruthenium red. The amount of capsular material produced was similar whether the media contained 0.5, 3.0, or 6% sucrose.

scholarly journals Comparative evaluation of different culture media for the isolation and identification of common urinary pathogens

2017 ◽  
Vol 5 (8) ◽  
pp. 3676 ◽  
Author(s):  
Aarti Chaturvedi ◽  
Ritu Garg ◽  
Varsha A. Singh
Keyword(s):  
Culture Media ◽  
Ambient Air ◽  
Work Load ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Continuous Increase ◽  
Gram Negative ◽  
Gram Positive Cocci

Background: Urinary tracts infections (UTIs) are one of the most common infections encountered in hospital as well as community settings. There is continuous increase in incidence of this infection leading to more consumption of antimicrobial drugs. Urine cultures occupy most of the workload of routine microbiology laboratories in developing country like India. Accurate and rapid identification of pathogens is the primary responsibility of a clinical microbiology laboratory.Methods: Mid-stream urine and catheterized samples were collected. Cultures were plated on blood agar, MacConkey agar and cysteine lactose electrolyte deficient media and incubated overnight at 35°C-37°C in ambient air. Colonies on the MacConkey agar, CLED agar and blood agar were also identified. The final identification of the isolates was done using standard identification protocol.  Antimicrobial susceptibility was performed by Kirby- Bauer disc diffusion test according to the CLSI guidelines.Results: Out of 500 urine samples processed, 211 samples showed significant growth, 24 samples showed polymicrobial growth and 265 samples were reported sterile.  Out of these 211, 199 showed pure growth and 12 showed mixed growths. Out of 199 pure growths, 126 were gram negative bacilli, 56 were gram positive cocci and 17 were yeast. All the gram-negative bacilli grown on all the media but most of the gram-positive cocci and yeast were unable to grow on Mac-Conkey agar and blood agar but grew successfully on CLED agar.Conclusions: So, in resource constrain laboratories, CLED agar can be used as media of choice for isolation of common uropathogens because it is user friendly, cost effective and decreases work load of the laboratories.

scholarly journals Isolation and Identification of Gram Positive Cocci Bacterial Pathogens from Sappurative Otitis Media Infections

2013 ◽  
Vol 37 (1) ◽  
pp. 129-133
Author(s):  
Weam Saad Al-Hamadany
Keyword(s):  
Otitis Media ◽  
Pure Culture ◽  
Bacterial Pathogen ◽  
Common Disease ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Suppurative Otitis Media ◽  
Bacteria And Fungi ◽  
Tract Infections ◽  
Gram Positive Cocci

Otitis Media (OM) is a common disease. It represents a serious problem mainly after winter outbreaks of respiratory tract infections (RTI); its consequences are hearing weakness; or even loss in adults and problems in speech in children. Gram-positive Cocci bacteria are among the causative pathogens. This study concerned with suppurative otitis media caused by gram-positive Cocci bacteria; either as pure culture or mixed with other pathogens. A total of 60 ear swabs were collected from Ear, Nose and Throat Department outpatients in AL-Nuaman hospital in Baghdad and cultured to isolate and identify the most common causative bacterial pathogen among gram positive Cocci. Staphylococcus aureus was the most common isolated bacteria (81.6%) representing (53.3%) as pure culture and (28.3%) as mixed. Streptococcus pneumonia was the second isolated pathogen (18.3%) representing (8.3%) as pure culture and (10%) as mixed. The mixed culture represented other pathogens like gram-negative bacteria and fungi.

scholarly journals Contamination of paper points used by students during preclinical and clinical endodontic procedures

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Pilar Angarita ◽  
Karen Mercedes Angarita
Keyword(s):  
Staphylococcus Epidermidis ◽  
Saline Solution ◽  
Anaerobic Bacteria ◽  
Absorption Capacity ◽  
Second Phase ◽  
Anaerobic Conditions ◽  
Gram Positive ◽  
Two Phases ◽  
Facultative Anaerobic Bacteria ◽  
Gram Positive Cocci

Objective: To determine the presence of facultative anaerobic bacteria in the paper points used by students and to perform a pilot test to determine whether sterilization of these materials influences their absorption capacity. Material and Methods: This study consists of two phases. The first is a descriptive phase where a representative sample of paper points (n = 72) was collected from the students and information about the points was voluntarily contributed. The points were placed in saline solution after they were collected, and mechanically shaken for 60 s. Then, 300 IU of the solution was seeded on blood agar in duplicates and incubated for 5 days under anaerobic conditions. The second phase was experimental, during which five paper points of each of the existing sizes were sterilized (Numbers: 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 70, and 80), and their capacity to absorb water was compared with that of the control or non-sterilized points. Results: The study determined that 22% (n=16) of the points were primarily contaminated by gram-positive bacilli, followed by gram-positive cocci, among which Staphylococcus epidermidis was identified. The presence of contamination was not significantly associated with the conditions of the paper points (p > 0.05). Furthermore, no significant effect (p>0.05) on the absorption capacity of these materials was detected in the sterilization test. Conclusion: Contamination was observed in the paper points used by the students, confirming the importance of implementing sterilization protocols. The sterilization protocols implemented in this study did not affect the absorption capacity of the points.KeywordsMicrobiology, Contamination; Bacteria; Anaerobic.

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