Products of anaerobic phloroglucinol degradation by Coprococcus sp. Pe15

1976 ◽  
Vol 22 (2) ◽  
pp. 159-164 ◽  
Author(s):  
Chii-Guary Tsai ◽  
Diane M. Gates ◽  
W. M. Ingledew ◽  
G. A. Jones

Under anaerobic conditions, resting cell suspensions of Coprococcus sp. Pe15 degraded 1 molecule of phloroglucinol to 2 molecules of acetic acid and 2 molecules of carbon dioxide. The organism metabolized the flavonoids rhamnetin and quercetin anaerobically in 20% rumen fluid medium but failed to grow under similar conditions at the expense of any of 39 other aromatic or flavonoid compounds tested.

2017 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
EllIN HARlIA HARlIA ◽  
MARlINA ET ◽  
MASITA R ◽  
RAHMAH KN

The natural methane formed by bacteria in anaerobic conditions is known as biogenic gas. Gas trapped in coal, formed through thermogenesis as well as biogenesisis known as coal-bed methane (CBM). The availability of organic material as decomposition of this material into methane is continuously required for the production of methane in the coal aquifer. The aim of this research was to investigate whether or not cattle feces bacteria were able to grow and produce methane in coal. Parameters measured were Volatile Fatty Acids (VFA) and the production of biogas, such as nitrogen, hydrogen, carbon dioxide, and methane. Explorative method was used and data obtained was analyzed by descriptive approach. The results showed that the bacteria found in the feces survived in the coal and produce biogas. On day 2 when the process was at the acidogenesis phase, it produced VFA with the largest component of acetic acid. Acetic acid would undergo decarboxylation and reduction of CO2 followed by reactions of H2and CO2 to produce methane (CH4) and carbon dioxide (CO2) as the final products. ,


1942 ◽  
Vol 6a (1) ◽  
pp. 45-52 ◽  
Author(s):  
G. J. Sigurdsson ◽  
A. J. Wood

The products of fermentation of glucose by "resting cell" suspensions of certain bacteria (Serratia, Achromobacter, and Micrococcus) isolated from decomposing cod muscle include lactic acid, acetic acid, formic acid, ethyl alcohol, carbon dioxide and small amounts of acetylmethylcarbinol. With increased acidity in the fermentation system there is a marked increase in the percentage of lactic acid formed, with a corresponding decrease in the other products. The optimum pH for the fermentation of glucose appears to be in the vicinity of 6.8—that is at, or near, the pH of fresh cod muscle.


1975 ◽  
Vol 21 (6) ◽  
pp. 794-801 ◽  
Author(s):  
Chii-Guary Tsai ◽  
G. A. Jones

Eight strains of rumen bacteria capable of degrading phloroglucinol (1,3,5-trihydroxybenzene) under anaerobic conditions were isolated from enrichment cultures of the bovine rumen microflora established in a prereduced medium containing 0.02 M phloroglucinol. Five of the strains were facultatively anaerobic Gram-positive streptococci which were identified as Streptococcus bovis. Three strains of obligately anaerobic Gram-positive cocci were assigned to the genus Coprococcus. Anaerobic cultures of the Streptococcus bovis strains in a 40% rumen fluid medium initially containing 0.02 M phloroglucinol degraded 50–80% of the substrate within 2 days, whereas cultures of the Coprococcus strains degraded more than 80% of the substrate under the same conditions. The Streptococcus bovis strains were incapable of degrading phloroglucinol in brain heart infusion or in the medium of de Man, Rogosa, and Sharpe (MRS broth) incubated aerobically.


In a comparison of muscles poisoned with mono-iodo-acetic acid (IAA) in the presence and in the absence of oxygen respectively, Lundsgaard (1930) found:- (1) That the spontaneous breakdown of phosphagen in poisoned resting muscle is much more rapid under anaerobic conditions. (2) That the onset of the characteristic contracture produced by IAA is accompanied always by an increase in the rate of oxygen consumption.


1946 ◽  
Vol 24f (1) ◽  
pp. 1-11 ◽  
Author(s):  
G. A. Adams

Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol. Aeration of similar mashes by passage of finely dispersed air or oxygen at the rate of 333 ml. per minute per litre of mash increased the rate of formation and yield of 2,3-butanediol but inhibited ethanol formation. However, the over-all time required for the completion of fermentation was not shortened from the usual 72 to 96 hr. required for unaerated mashes. There was no evidence of a shift from fermentative to oxidative dissimilation. Under aerobic conditions, the final butanediol–ethanol ratio was approximately 3:1. Anaerobic conditions, as produced by the passage of nitrogen or hydrogen through the mash, increased the rate of formation of both butanediol and ethanol and shortened the fermentation time to about 48 hr. Under these conditions, the butanediol–ethanol ratio was reduced to about 1.3:1.0. Carbon dioxide gave a butanediol–ethanol ratio resembling that of anaerobic fermentation but did not reduce fermentation time.


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