Sur l'activité antibiotique de l'Armillaria mellea

1971 ◽  
Vol 17 (11) ◽  
pp. 1395-1399 ◽  
Author(s):  
Claude Richard

Antibiotic activity of Armillaria mellea (Vahl ex Fr.) Kummer towards many fungi and bacteria, including some very important plant pathogens, was clearly established. Preliminary extraction trials have been negative. No direct relationship was found between the bioluminescence of A. mellea and its antibiotic activity. Clitocybe tabescens (Scop, ex Fr.) Bres. also possesses antibiotic capability, but to a much lower degree.

1954 ◽  
Vol 32 (5) ◽  
pp. 705-735 ◽  
Author(s):  
Z. A. Patrick

In an attempted evaluation of the importance of soil antagonisms as a possible factor in the different survival capabilities of some bacterial plant pathogens in the soil environment, a comparison was made of the numbers of antagonists detected when different plant pathogenic species were used as test organisms in determining the "antibiotic potential" of nine "virgin" soils. It was found that there are present among the soil flora a great abundance of microorganisms intrinsically capable of antagonizing most of the bacterial pathogens tested and only for a few species are such antagonists relatively rare. There were great differences in the number of isolates antagonistic to the different pathogenic species, even in the same genus, and there seemed to be a correlation between the numbers of antagonists, as found here, and the capability of a species to maintain itself for long periods in the soil. For the most part the Xanthomonas species appeared to be most sensitive to the antagonistic soil microflora while the soft-rot-causing Erwinia species were most resistant.A comparative study of the antibiotic activity of 120 of the most active antagonistic isolates tested against 28 bacterial plant pathogens showed that each antagonist was characterized by a specific antibacterial spectrum and those antagonists having the most intense antibiotic activity usually inhibited the greatest number of bacterial species. Many antagonists were highly specific, affecting only certain groups or even certain species. The high specificity which characterized some of the antibiotic reactions was used to separate sharply, consistently, and with minimum effort such closely related species as E. carotovora and E. atroseptica or X. corylina and X. juglandis.


1950 ◽  
Vol 28c (6) ◽  
pp. 690-698 ◽  
Author(s):  
G. B. Landerkin ◽  
Jane R. G. Smith ◽  
A. G. Lochhead

Of 660 cultures of actinomycetes isolated on a nonselective basis from soils from five locations in Northern Canada, 404 strains, or 61.2%, showed antagonism against at least one of eight test organisms consisting of five bacteria and three plant pathogenic fungi. The degree of activity ranged from slight to strong, with the number of test organisms inhibited by any single culture varying from one to six. In all, 49 different antibiotic spectra were observed. Activity against one or more of the pathogenic fungi was shown by 138 cultures, 20.9% of the isolates. The percentage of active cultures was greater, and the degree of inhibition much more marked, against Helminthosporium sativum than against Fusarium culmorum or Fusarium lini. The percentage of active isolates varied with the location and with the depth of the soil. It is suggested that the high proportion of actinomycetes with antagonistic properties occurring in northern soils may be related to the lower degree of plant development in such regions.


1988 ◽  
Vol 66 (11) ◽  
pp. 2338-2346 ◽  
Author(s):  
L. R. Schreiber ◽  
Garold F. Gregory ◽  
C. R. Krause ◽  
J. M. Ichida

Antibiotic substances produced by a Bacillus subtilis isolate from the xylem of an American elm (Ulmus americana L.) inhibited several fungal plant pathogens including the Dutch elm disease pathogen, Ceratocystis ulrni. Bioassays indicated the bacterium produced a diffusible, methanol-soluble antibiotic on potato dextrose agar at 30 °C. Cellulose powder column chromatography separated the antibiotic, which was further purified on a Sephadex LH-20 column, yielding two peaks of antibiotic activity, BS1 and BS2. These were further purified by paper chromatography. High-performance liquid chromatography, using C-18 reverse phase chromatography under isocratic conditions, resulted in five 280-nm absorbing peaks. Only one peak contained antibiotic activity. Upon ultrafiltration, the antibiotics passed through a 500 mol. wt. filter. The antibiotic was soluble in water, absolute methanol, ethanol, and chloroform but not in hexane or petroleum ether. Antimicrobial activity differed from that of other B. subtilis antibiotics including iturin A, bacillomycin L, mycosubtilin, fengycin, and bacilysin. Ceratocystis ulmi exposed to BS1 produced short, twisted, and swollen hyphae with irregularly thickened cell walls.


2008 ◽  
Vol 98 (5) ◽  
pp. 547-554 ◽  
Author(s):  
A. J. Downer ◽  
D. Crohn ◽  
B. Faber ◽  
O. Daugovish ◽  
J. O. Becker ◽  
...  

Ground green waste is used as mulch in ornamental landscapes and for tree crops such as avocados. Survival of Armillaria mellea, Phytophthora cinnamomi, Sclerotinia sclerotiorum, and Tylenchulus semipenetrans was assessed for 8 weeks within unturned piles of either recently ground or partially composted green waste. S. sclerotiorum survived at the pile surface and at 10, 30, and 100 cm within the pile for the entire 8 weeks in both fresh green waste (FGW) and aged green waste (AGW). A. mellea and T. semipenetrans did not survive more than 2 days in FGW, while P. cinnamomi persisted for over 21 days in FGW. AGW was less effective in reducing pathogen viability than FGW, most likely because temperatures in AGW peaked at 45°C compared with 70°C in FGW. Survival modeling curves based on pile temperatures indicate the time to inactivate 10 propagules of pathogens was 11, 30, 363, and 50 days for A. mellea, P. cinnamomi, S. sclerotiorum, and T. semipenetrans, respectively. Sclerotia-forming pathogens pose the greatest risk for escape; to ensure eradication of persistent fungi, green waste stockpiles should be turned intermittently to mix pile contents and move pathogen propagules to a location within the pile where they are more likely to be killed by heat, microbial attack, or chemical degradation.


mSphere ◽  
2021 ◽  
Author(s):  
Adrien Biessy ◽  
Amy Novinscak ◽  
Renée St-Onge ◽  
Geneviève Léger ◽  
Antoine Zboralski ◽  
...  

Plant-beneficial phenazine-producing Pseudomonas spp. are effective biocontrol agents, thanks to the broad-spectrum antibiotic activity of the phenazine antibiotics they produce. These bacteria have received considerable attention over the last 20 years, but most studies have focused only on the ability of a few genotypes to inhibit the growth of a limited number of plant pathogens.


1994 ◽  
Vol 144 ◽  
pp. 421-426
Author(s):  
N. F. Tyagun

AbstractThe interrelationship of half-widths and intensities for the red, green and yellow lines is considered. This is a direct relationship for the green and yellow line and an inverse one for the red line. The difference in the relationships of half-widths and intensities for different lines appears to be due to substantially dissimilar structuring and to a set of line-of-sight motions in ”hot“ and ”cold“ corona regions.When diagnosing the coronal plasma, one cannot neglect the filling factor - each line has such a factor of its own.


Author(s):  
C. W. Mims ◽  
E. A. Richardson

The advantages of freeze substitution fixation over conventional chemical fixation for preservation of ultrastructural details in fungi have been discussed by various authors. As most ascomycetes, basidiomycetes and deuteromycetes do not fix well using conventional chemical fixation protocols, freeze substitution has attracted the attention of many individuals interested in fungal ultrastructure. Thus far most workers using this technique on fungi have concentrated on thin walled somatic hyphae. However, in our laboratory we have experimented with the use of freeze substitution on a variety of fungal reproductive structures and spores with promising results.Here we present data on freeze substituted samples of sporangia of the zygomycete Umbellopsis vinacea, basidia of Exobasidium camelliae var. gracilis, developing teliospores of the smut Sporisorium sorghi, germinating teliospores of the rust Gymnosporangium clavipes, germinating conidia of the deuteromycete Cercosporidium personatum, and developing ascospores of Ascodesmis nigricans.Spores of G. clavipes and C. personatum were deposited on moist pieces of sterile dialysis membrane where they hydrated and germinated. Asci of A. nigricans developed on pieces of dialysis membrane lying on nutrient agar plates. U. vinacea was cultured on small pieces of agar-coated wire. In the plant pathogens E. camelliae var. gracilis and S. sorghi, a razor blade was used to remove smal1 pieces of infected host issue. All samples were plunged directly into liquid propane and processed for study according to Hoch.l Samples on dialysis membrane were flat embedded. Serial thin sections were cut using a diamond knife, collected on slot grids, and allowed to dry down onto Formvar coated aluminum racks. Sections were post stained with uranyl acetate and lead citrate.


Author(s):  
Stefan Krause ◽  
Markus Appel

Abstract. Two experiments examined the influence of stories on recipients’ self-perceptions. Extending prior theory and research, our focus was on assimilation effects (i.e., changes in self-perception in line with a protagonist’s traits) as well as on contrast effects (i.e., changes in self-perception in contrast to a protagonist’s traits). In Experiment 1 ( N = 113), implicit and explicit conscientiousness were assessed after participants read a story about either a diligent or a negligent student. Moderation analyses showed that highly transported participants and participants with lower counterarguing scores assimilate the depicted traits of a story protagonist, as indicated by explicit, self-reported conscientiousness ratings. Participants, who were more critical toward a story (i.e., higher counterarguing) and with a lower degree of transportation, showed contrast effects. In Experiment 2 ( N = 103), we manipulated transportation and counterarguing, but we could not identify an effect on participants’ self-ascribed level of conscientiousness. A mini meta-analysis across both experiments revealed significant positive overall associations between transportation and counterarguing on the one hand and story-consistent self-reported conscientiousness on the other hand.


Homeopathy ◽  
2020 ◽  
Author(s):  
Thais Moraes Ferreira ◽  
Mariana Zandomênico Mangeiro ◽  
Alexandre Macedo Almeida ◽  
Ricardo Moreira Souza

Abstract Background There are relatively few scientific works on the use of homeopathy to manage plant pathogens, particularly nematodes. A handful of studies focused on Meloidogyne spp. parasitizing vegetables have brought contradictory results on nematode control and enhancement of plant tolerance to parasitism. Objective Our goal was to assess the effect of Cina—a well-known anti-nematode ingredient—on Meloidogyne enterolobii parasitizing lettuce. Methods Cina was applied daily on nematode-inoculated plants, from the seedling stage until harvest. We tested an evenly spaced range of Hahnemannian concentrations (c), which were applied though irrigation with a constant dose of the ingredient. Several absolute and relative controls were employed to allow the assessment of the effect of Cina on nematode reproduction and lettuce growth. Results Cina affected growth of non-parasitized plants, both positively and negatively; this effect was modulated by the c applied and the thermal stress suffered by the plants in one of the assays. The effect of Cina on the growth of nematode-parasitized plants was neutral or negative. Cina reduced nematode reproduction by 25–36%. Conclusion Based on the moderate negative effect of Cina on M. enterolobii reproduction, it seems this ingredient may be useful as a complementary strategy for Meloidogyne control. But Cina did not enhance the tolerance of lettuce to Meloidogyne spp.


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