Production, partial purification, and antimicrobial activity of a novel antibiotic produced by a Bacillus subtilis isolate from Ulmus americana

1988 ◽  
Vol 66 (11) ◽  
pp. 2338-2346 ◽  
Author(s):  
L. R. Schreiber ◽  
Garold F. Gregory ◽  
C. R. Krause ◽  
J. M. Ichida
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
High Performance ◽  
Plant Pathogens ◽  
Antibiotic Activity ◽  
Dutch Elm Disease ◽  
Absolute Methanol ◽  
Partial Purification ◽  
Ulmus Americana ◽  
Fungal Plant Pathogens

Antibiotic substances produced by a Bacillus subtilis isolate from the xylem of an American elm (Ulmus americana L.) inhibited several fungal plant pathogens including the Dutch elm disease pathogen, Ceratocystis ulrni. Bioassays indicated the bacterium produced a diffusible, methanol-soluble antibiotic on potato dextrose agar at 30 °C. Cellulose powder column chromatography separated the antibiotic, which was further purified on a Sephadex LH-20 column, yielding two peaks of antibiotic activity, BS1 and BS2. These were further purified by paper chromatography. High-performance liquid chromatography, using C-18 reverse phase chromatography under isocratic conditions, resulted in five 280-nm absorbing peaks. Only one peak contained antibiotic activity. Upon ultrafiltration, the antibiotics passed through a 500 mol. wt. filter. The antibiotic was soluble in water, absolute methanol, ethanol, and chloroform but not in hexane or petroleum ether. Antimicrobial activity differed from that of other B. subtilis antibiotics including iturin A, bacillomycin L, mycosubtilin, fengycin, and bacilysin. Ceratocystis ulmi exposed to BS1 produced short, twisted, and swollen hyphae with irregularly thickened cell walls.

scholarly journals The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciąg ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

AbstractPseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

Cellular and environmental factors influencing biofilm formation and colonization of plant tissue by a beneficial strain of bacteria, Pseudomonas donghuensis P482.

2020 ◽  
Author(s):  
Magdalena Rajewska ◽  
Marta Matuszewska ◽  
Sylwia Jafra
Keyword(s):  
Antimicrobial Activity ◽  
Environmental Factors ◽  
Biofilm Formation ◽  
Genetic Background ◽  
Plant Pathogens ◽  
Knock Out ◽  
Factors Influencing ◽  
Fungal Plant Pathogens ◽  
Artificial Surfaces ◽  
Strain Type

<p>The ability to colonize different environmental niches by bacteria is most often determined by the ability to form biofilms - complex, multicellular communities. This, in turn, depends on both cellular and extracellular factors such as genetic background of the strain, type of surface (biotic or abiotic) to which bacteria attach, availability of nutrients, temperature, <em>etc</em>. <em>Pseudomonas donghuensis</em> P482 strain is a little-known isolate from tomato rhizosphere, exhibiting antimicrobial activity towards bacterial and fungal plant pathogens. Studies have shown that it efficiently colonizes plant rhizosphere and forms biofilm on artificial surfaces. Which genetic or environmental factors underlie the mechanism of biofilm formation were yet to be elucidated. The presented research aimed at identifying those factors. Basing on the analysis of genome, knock-out mutants of the P482 strain were constructed in the genes potentially involved in biofilm formation and further analyzed for motility, colony morphology, attachment to artificial surfaces in different culture conditions, and colonization of maize and tomato rhizosphere.</p>

Interactions with plant pathogens influence lipopeptides production and antimicrobial activity of Bacillus subtilis strain PTB185

2021 ◽  
Vol 154 ◽  
pp. 104497
Author(s):  
Louis Cossus ◽  
Florence Roux-Dalvai ◽  
Isabelle Kelly ◽  
Thi Thuy An Nguyen ◽  
Hani Antoun ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Plant Pathogens ◽  
Subtilis Strain

scholarly journals Necessity requires no decision: carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciag ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

Abstract Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7‑hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

scholarly journals Changes in Peptaibol Production of Trichoderma Species during In Vitro Antagonistic Interactions with Fungal Plant Pathogens

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 730
Author(s):  
Parisa Rahimi Tamandegani ◽  
Tamás Marik ◽  
Doustmorad Zafari ◽  
Dóra Balázs ◽  
Csaba Vágvölgyi ◽  
...  
Keyword(s):  
High Performance ◽  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Alternaria Solani ◽  
Pathogenic Fungi ◽  
Isobutyric Acid ◽  
Reversed Phase ◽  
Trichoderma Species ◽  
Fungal Plant Pathogens

Trichoderma species are widely used as biofungicides for the control of fungal plant pathogens. Several studies have been performed to identify the main genes and compounds involved in Trichoderma–plant–microbial pathogen cross-talks. However, there is not much information about the exact mechanism of this profitable interaction. Peptaibols secreted mainly by Trichoderma species are linear, 5–20 amino acid residue long, non-ribosomally synthesized peptides rich in α-amino isobutyric acid, which seem to be effective in Trichoderma–plant pathogenic fungus interactions. In the present study, reversed phase (RP) high-performance liquid chromatography (HPLC) coupled with electrospray ionization (ESI) mass spectrometry (MS) was used to detect peptaibol profiles of Trichoderma strains during interactions with fungal plant pathogens. MS investigations of the crude extracts deriving from in vitro confrontations of Trichoderma asperellum and T. longibrachiatum with different plant pathogenic fungi (Fusarium moniliforme, F. culmorum, F. graminearum, F. oxysporum species complex, Alternaria solani and Rhizoctonia solani) were performed to get a better insight into the role of these non-ribosomal antimicrobial peptides. The results revealed an increase in the total amount of peptaibols produced during the interactions, as well as some differences in the peptaibol profiles between the confrontational and control tests. Detection of the expression level of the peptaibol synthetase tex1 by qRT-PCR showed a significant increase in T. asperellum/R. solani interaction in comparison to the control. In conclusion, the interaction with plant pathogens highly influenced the peptaibol production of the examined Trichoderma strains.

scholarly journals Cinnabarinic Acid from Trametes Coccinea Fruiting Bodies Exhibits Antibacterial Activity Through Inhibiting the Biofilm Formation

2021 ◽  
Author(s):  
Merilin Kakoti ◽  
Samim Dullah ◽  
Dibya Jyoti Hazarika ◽  
Madhumita Barooah ◽  
Robin Chandra Boro
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
High Performance ◽  
Antidiabetic Activity ◽  
Ionization Mass ◽  
Fruiting Bodies ◽  
Bioactive Properties

Abstract Wild mushrooms are rich sources of natural compounds with potent bioactive properties. Several important metabolites have been reported from mushrooms, which possess clinically important bioactive properties like antibacterial activity, anticancer activity, antidiabetic activity and antioxidant activity. In this study, we have evaluated the antimicrobial activity of Trametes coccinea fruiting body extracts against different bacterial isolates viz. Bacillus subtilis, Bacillus cereus and Escherichia coli. Fruiting bodies of three T. coccinea samples, of which two were collected from Santipur, Arunachal Pradesh and one collected from Jorhat, Assam, were used for extraction using methanol. The extracts showed potent antimicrobial activity against all the test bacteria. Minimum Inhibitory Concentration (MIC) of the extracts against Bacillus subtilis, Bacillus cereus and Escherichia coli was recorded as 400 µg/ml, 400 µg/ml and 300 µg/ml respectively. Further, the bioactive compounds of the extract were separated and detected using Thin Layer Chromatography (TLC). Presence of Cinnabarinic acid (CBA), a potent antimicrobial compound was detected in TLC, which was further confirmed through High Performance Liquid Chromatography (HPLC) and Electrospray Ionization-Mass Spectrometry (ESI-MS). Cinnabarinic acid was able to inhibit the formation of biofilms in Bacillus subtilis and B. cereus, suggesting that the compound can be beneficial in the management of biofilm based antimicrobial resistance.

Peptidotriazoles with antimicrobial activity against bacterial and fungal plant pathogens

Peptides ◽  
2012 ◽  
Vol 33 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Imma Güell ◽  
Lluís Micaló ◽  
Laura Cano ◽  
Esther Badosa ◽  
Rafael Ferre ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens
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