Effect of culture medium ions on chromate reduction by resting cells of Agrobacterium radiobacter

1993 ◽  
Vol 39 (3) ◽  
Author(s):  
Santiago LLovera ◽  
Ramon Bonet ◽  
MariaDolores Simon-Pujol ◽  
Francisco Congregado
Keyword(s):  
Culture Medium ◽  
Chromate Reduction ◽  
Resting Cells ◽  
Agrobacterium Radiobacter

scholarly journals Chromate Reduction by Resting Cells of Agrobacterium radiobacter EPS-916

1993 ◽  
Vol 59 (10) ◽  
pp. 3516-3518 ◽  
Author(s):  
Santiago Llovera ◽  
Ramon Bonet ◽  
Maria D. Simon-Pujol ◽  
Francisco Congregado
Keyword(s):  
Chromate Reduction ◽  
Resting Cells ◽  
Agrobacterium Radiobacter

The metabolism of p-fluorophenylacetic acid by a Pseudomonas sp. I. Isolation and identification of intermediates in degradation

1971 ◽  
Vol 17 (5) ◽  
pp. 635-644 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Sole Carbon Source ◽  
Culture Medium ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Spectroscopic Techniques ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Hydroxyphenylacetic Acid ◽  
Organic Fluorine

A Pseudomonas sp. capable of growing on p-fluorophenylacetic acid as sole carbon source has been isolated using the enrichment culture technique. All the organic fluorine is released into the culture medium as fluoride ion during growth. A number of fluorinated intermediates have been isolated from the culture medium when resting cells were incubated with the substrate. Using infrared, nuclear magnetic resonance, and mass spectroscopic techniques together with chemical degradative procedures, these have been identified as D(+)-monofluorosuccinic acid, trans-3-fluoro-3-hexenedioic acid, (−)-4-carboxymethyl-4-fluorobutanolide, 4-fluoro-2-hydroxyphenylacetic acid, and 4-fluoro-3-hydroxyphenylacetic acid.

The separation of alginate biosynthesis and acetylation in Pseudomonas syringae

1998 ◽  
Vol 44 (4) ◽  
pp. 394-398 ◽  
Author(s):  
Jin W Lee ◽  
F Day
Keyword(s):  
Pseudomonas Syringae ◽  
Culture Medium ◽  
Enzyme System ◽  
Indirect Evidence ◽  
Resting Cells ◽  
Resting Cell ◽  
Degree Of Acetylation ◽  
Induced Mutants ◽  
Alginate Acetylation ◽  
Alginate Biosynthesis

Seaweed alginate was acetylated by resting cells of Pseudomonas syringae subsp. phaseolicola ATCC 19304. Physiological studies on this strain and its UV-induced mutants showed no correlation between bacterial alginate biosynthesis and acetylation. Specific yields of alginate and degree of acetylation in these polymers varied with strain and culture medium. This was indirect evidence that alginate biosynthesis is separate from polysaccharide acetylation. It indicated that the enzyme system involved in alginate biosynthesis was not directly linked to alginate acetylation and explained why microbial acetylation of seaweed alginates was possible.Key words: resting cell, Pseudomonas syringae, acetylation, bacterial alginate, seaweed alginate.

scholarly journals Melatonin biosynthesis and metabolism in peripheral blood mononuclear leucocytes

1991 ◽  
Vol 280 (3) ◽  
pp. 727-731 ◽  
Author(s):  
L M E Finocchiaro ◽  
V E Nahmod ◽  
J M Launay
Keyword(s):  
Mass Spectrometry ◽  
Peripheral Blood ◽  
Culture Medium ◽  
Human Peripheral Blood ◽  
Resting Cells ◽  
Two Dimensional ◽  
Peripheral Blood Mononuclear ◽  
Ifn Gamma ◽  
Mononuclear Leucocytes ◽  
Tryptophan Hydroxylation

Cultured human peripheral blood mononuclear leucocytes (PBML) were able to synthesize indoleamines, including melatonin, and were also able to convert melatonin taken up from the incubation medium into N-acetyl-5-hydroxytryptamine (NAHT) and 5-hydroxytryptamine (5-HT). These compounds were analysed by h.p.l.c., and melatonin was additionally characterized by two-dimensional t.l.c., mass spectrometry and radioimmunoassay. Only hydroxyindoles were detected by h.p.l.c. in unstimulated PBML culture. Sustained stimulation by melatonin or interferon-gamma (IFN-gamma) increased markedly the basal production of 5-HT. IFN-gamma- or 5-HT-stimulated (but not resting) cells produced NAHT and melatonin. Furthermore, the addition of melatonin to the culture medium strongly enhanced NAHT and 5-HT production without affecting tryptophan hydroxylation, suggesting the possibility of direct or indirect transformation of melatonin into NAHT and 5-HT.

The metabolism of p-fluorobenzoic acid by a Pseudomonas sp.

1971 ◽  
Vol 17 (8) ◽  
pp. 1015-1023 ◽  
Author(s):  
D. B. Harper ◽  
E. R. Blakley
Keyword(s):  
Metabolic Pathways ◽  
Culture Medium ◽  
Resting Cells ◽  
Pseudomonas Sp ◽  
Metabolic Intermediates ◽  
Organic Fluorine

A species of Pseudomonas previously used to study the degradation of p-fluorophenylacetic acid was used to investigate the degradation of p-fluorobenzoic acid. During growth on the latter substrate all organic fluorine was released into the culture medium as fluoride. Several metabolic intermediates were isolated from the culture medium of resting cells. The major compounds have been identified as 4-fluorocatechol, β-acetylacrylic acid, (+)-4-carboxymethyl-4-fiuorobut-2-enolide, (−)-4-carboxymethyl-but-2-enolide, and β-ketoadipic acid. A small quantity of a compound tentatively identified as β-fiuoroacrylic acid was also isolated. On the basis of these findings, together with respiratory studies on p-fluorobenzoic acid grown cells with various substrates, two metabolic pathways are proposed which involve elimination of fluorine at either of two alternative stages in the breakdown of p-fluorobenzoic acid.

scholarly journals Methods of biological treatment intensification aimed at removal of heavy metals

Vestnik MGSU ◽  
2020 ◽  
pp. 847-858
Author(s):  
Irina I. Ivanenkо ◽  
Antonina M. Novikova
Keyword(s):  
Biological Treatment ◽  
Culture Medium ◽  
Chemical Elements ◽  
Electron Acceptors ◽  
Laboratory Research ◽  
Editorial Team ◽  
Aerobic Bacteria ◽  
Chromate Reduction ◽  
Treatment Technology ◽  
Reduction Sequence

Introduction. The application of microorganisms, capable of using chemical elements with variable valency as terminal electron acceptors, in the wastewater treatment technology can improve the ecological and energy efficiency of biological treatment plants. Materials and methods. The co-authors employed the analytical generalization of findings, including the overview of literary sources, laboratory researches involving standard and advanced methodologies and analytical equipment. Results. Cr(6+) Мn(4+), highly concentrated in wastewaters of versatile production facilities, deserve attention as terminal electron acceptors. The ability of Асіnеtobacter, a non-adapted aerobic bacteria, to reduce Мn(4+), Сr(6+) in the culture medium under a layer of vasseline oil, was studied in the laboratory environment. In the course of the laboratory research, different aspects of Сr(6+)- и Мn(4+) reduction were studied, namely, the bacteria’s chromate reduction ability, as Сr(6+) concentration change curves were obtained for the process of reduction; the influence of Сr(6+) and Мn(2+) reductions on processes of chromate and manganese reduction was identified; water treatment efficiency boosted by Сr(6+) was identified; changes in the reduction sequence in case of their co-presence in the culture medium were checked; the sequence of Сr(6+) and Мn(4+) reduction in case of their co-cultivation together with the bacteria was identified, as well. A succession of experiments with Pseudomonas bacteria was held to validate the results. Conclusions. The experiments have proven that aerobic bacteria, including Асіnеtobacter, P. aeruginosa P-1, P. flurescens var. Pseudo-iodinum P-11, P. Mendocina P-13, P. stutzeri P-19, can develop anaerobic respiration ability under certain conditions. The co-authors have identified that Сr(6+)- and Мn(4+) reduction is applicable by microorganisms, belonging to varied taxonomic groups, in anaerobic cultivation environments, if Сr(6+)- and Мn(4+) act as terminal acceptors of electrons; the co-authors have also reconstructed the reduction sequence: denitrifying bacteria use МnО2 much more efficiently than Сr(6+). Microbial chromate reduction precedes Мn(4+) reduction, while products of their metabolism are less toxic. Acknowledgements. The co-authors would like to express gratitude to the executives of the St. Petersburg University of Architecture and Civil Engineering for the research grant and to all reviewers and the editorial team for the publication of this material.

Chromate reduction by resting cells of Achromobacter sp. Ch-1 under aerobic conditions

2007 ◽  
Vol 42 (6) ◽  
pp. 1028-1032 ◽  
Author(s):  
Zemin Ma ◽  
Wenjie Zhu ◽  
Huaizhong Long ◽  
Liyuan Chai ◽  
Qingwei Wang
Keyword(s):  
Chromate Reduction ◽  
Resting Cells ◽  
Aerobic Conditions ◽  
Achromobacter Sp

scholarly journals Removal Efficiency of Cr6+by IndigenousPichiasp. Isolated from Textile Factory Effluent

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Pablo M. Fernández ◽  
María M. Martorell ◽  
Julia I. Fariña ◽  
Lucia I. C. Figueroa
Keyword(s):  
Culture Medium ◽  
Incubation Temperature ◽  
Reductase Activity ◽  
Chromate Reduction ◽  
Intact Cells ◽  
Chromate Reductase ◽  
Optimum Ph ◽  
Associated Proteins ◽  
Textile Factory

Resistance of the indigenous strainsP. jadiniiM9 andP. anomalaM10, to high Cr6+concentrations and their ability to reduce chromium in culture medium was studied. The isolates were able to tolerate chromium concentrations up to 104 μg mL−1. Growth and reduction of Cr6+were dependent on incubation temperature, agitation, Cr6+concentration, and pH. Thus, in both studied strains the chromium removal was increased at 30°C with agitation. The optimum pH was different, with values of pH 3.0 and pH 7.0 in the case ofP. anomalaM10 and pH 7.0 usingP. jadiniiM9. Chromate reduction occurred both in intact cells (grown in culture medium) as well as in cell-free extracts. Chromate reductase activity could be related to cytosolic or membrane-associated proteins. The presence of a chromate reductase activity points out a possible role of an enzyme in Cr6+reduction.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

Structural aspects of osmoregulation in porphyra

1978 ◽  
Vol 36 (2) ◽  
pp. 412-413
Author(s):  
C. Wiencke ◽  
A. Lauchli
Keyword(s):  
Culture Medium ◽  
Point Of View ◽  
Chemical Fixation ◽  
Cellular Organization ◽  
Osmotic Adaptation ◽  
Osmotic Balance ◽  
Activity Of Enzymes ◽  
Osmotic Agents ◽  
Vacuolar System ◽  
Structural Aspects

Osmoregulatory mechanisms in algae were investigated mainly from a physiological point of view (KAUSS 1977, HELLEBUST 1976). In Porphyra two osmotic agents, i. e. floridoside/isofloridoside (KAUSS 1968) and certain ions, such as K+ and Na+(EPPLEY et al. 1960) are considered for osmotic balance. Accumulations of ions (particularly Na+) in the cytoplasm during osmotic adaptation is improbable, because the activity of enzymes is generally inhibited by high ionic concentrations (FLOWERS et al. 1977).The cellular organization of Porphyra was studied with special emphasis on the development of the vacuolar system under different hyperosmotic conditions. Porphyra was cultivated at various strengths of the culture medium ASP 12 (PROVASOLI 1961) ranging from normal to 6 times concentrated (6x) culture medium. Por electron microscopy freeze fracturing was used (specimens fixed in 2% glutaraldehyde and incubated in 30% glycerol, preparation in a BALZERS BA 360 M apparatus), because chemical fixation gave poor results.

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