COMPARATIVE STUDIES OF IMMUNOGENIC PROPERTIES OF TRYPANOSOMA BRUCEI INACTIVATED WITH β-PROPIOLACTONE AND WITH SOME OTHER INACTIVATING AGENTS

1967 ◽  
Vol 13 (7) ◽  
pp. 743-747 ◽  
Author(s):  
M. A. Soltys
Keyword(s):  
Trypanosoma Brucei ◽  
Comparative Studies ◽  
The Other ◽  
Passive Immunity ◽  
Distilled Water ◽  
Other Hand ◽  
Homologous Strain ◽  
Protective Antibodies ◽  
Immunogenic Properties ◽  
Young Mice

Success in immunizing animals with dead trypanosomes depends on both the method of inactivating trypanosomes and the strain used for immunization. Comparative studies with various inactivating agents showed that β-propio-lactone and formalin are superior to phenol, heat at 56 °C, and lysis in distilled water. A vaccine containing Trypanosoma brucei with frequent passages through mice and not exposed to antibodies could elicit in animals protective antibodies and resistance to a challenge with a homologous strain. Passive immunity was shown in young mice born of vaccinated mothers. On the other hand, no active or passive immunity was demonstrated when animals were immunized with a vaccine prepared from a variant of T. brucei after passage through rabbits or sheep.

Activity of Plasmin and Streptokinase-Activator on Substituted Arginine and Lysine Esters

1966 ◽  
Vol 16 (01/02) ◽  
pp. 018-031 ◽  
Author(s):  
S Sherry ◽  
Norma Alkjaersig ◽  
A. P Fletcher
Keyword(s):  
Molecular Structure ◽  
Methyl Ester ◽  
Comparative Studies ◽  
Esterase Activity ◽  
Methyl Esters ◽  
Hydrolytic Activity ◽  
The Other ◽  
Other Hand

SummaryComparative studies have been made of the esterase activity of plasmin and the streptokinase-activator of plasminogen on a variety of substituted arginine and lysine esters. Human plasmin preparations derived by different methods of activation (spontaneous in glycerol, trypsin, streptokinase (SK) and urokinase) are similar in their esterase activity; this suggests that the molecular structure required for such esterase activity is similar for all of these human plasmins. Bovine plasmin, on the other hand, differs from human plasmin in its activity on several of the substrates studied (e.g., the methyl esters of benzoyl arginine and tosyl, acetyl and carbobenzoxy lysine), a finding which supports the view that molecular differences exist between the two animal plasmins. The streptokinase-activator hydrolyzes both arginine and lysine esters but the ratios of hydrolytic activity are distinct from those of plasmin and of other activators of plasminogen. The use of benzoyl arginine methyl ester as a substrate for the measurement of the esterase activity of the streptokinase-activator is described.

scholarly journals Using homogenization, sonication and thermo-sonication to inactivate fungi

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2020 ◽  
Author(s):  
Daniela Campaniello ◽  
Antonio Bevilacqua ◽  
Milena Sinigaglia ◽  
Maria Rosaria Corbo
Keyword(s):  
High Pressure ◽  
Thermal Treatment ◽  
The Other ◽  
Distilled Water ◽  
High Pressure Homogenization ◽  
Antifungal Effect ◽  
Other Hand

Ultrasound (US), Thermo-sonication (TS) and High Pressure Homogenization (HPH) were studied as tools to inactivate the spores ofPenicilliumspp. andMucorspp. inoculated in distilled water. For US, the power ranged from 40% to 100%, pulse from 2 to 10 s, and duration of the treatment from 2 to 10 min. TS was performed combining US (40–80% of power, for 8 min and pulse of 2 s) with a thermal treatment (50, 55 and 60°C at 4, 8 and 12 min). Homogenization was done at 30–150 MPa for 1, 2 and 3 times. Power was the most important factors to determine the antifungal effect of US and TS towards the conidia ofPenicilliumspp.; on the other hand, in US treatmentsMucorspp. was also affected by pulse and time. HPH exerted a significant antifungal effect only if the highest pressures were applied for 2–3 times.

scholarly journals UNDERSTANDING OF UZBEK AND KOREAN AUXILIARY VERBS

2021 ◽  
Author(s):  
Yeon SANGHEUM ◽  
Keyword(s):  
Comparative Studies ◽  
The Other ◽  
Contrast Study ◽  
Auxiliary Verbs ◽  
Corpus Construction ◽  
Other Hand ◽  
Sentence Construction ◽  
Auxiliary Verb ◽  
Structural Aspects

Uzbek and Korean are characterized by agglutination. When comparing and contrasting the two languages, we can find quite a few similarities in the conjugation of verbs, especially auxiliary verbs, where the characteristics of the agglutinating language are most prominent. In the use of auxiliary verbs, the two languages ​​are similar in semantically as well as in simple structural aspects, and there are many cases where the same meaning is expressed using the auxiliary verb. On the other hand, there are differences as well, but there is still a lack of comparative studies between the two languages ​​on the corresponding grammar item. In addition, errors in the most common and widely used Google translator can also be found. Although there were no major problems in conveying simple meanings, sentence construction using auxiliary verbs was not performed properly. By briefly introducing these problems, it was found that the necessity for contrast study and corpus construction between the two languages was required.

scholarly journals Spectroscopy Study of Honey Pineapple Peels Extracted in Different Solvents

2021 ◽  
Vol 3 (1) ◽  
pp. 32-35
Author(s):  
Yehezkiel Steven Kurniawan ◽  
Edi Setiyono ◽  
Marcelinus Alfasisurya Setya Adhiwibawa ◽  
Krisfian Tata Aneka Priyangga ◽  
Leny Yuliati
Keyword(s):  
Carboxylic Acid ◽  
Visible Region ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
The Other ◽  
Distilled Water ◽  
Spectroscopy Study ◽  
Absorption Signal ◽  
Other Hand

In the present work, we investigated the extract of honey pineapple peels in distilled water, ethanol, and acetone solvents. The spectroscopy study of each extract was performed using a Fourier transform infrared (FTIR) spectrometer, an ultraviolet-visible (UV-Vis) spectrophotometer, and a spectrofluorometer. The FTIR spectrum of the distilled water extract indicated that the distilled water extract may contain alcohol or carboxylic acid compounds. Meanwhile, the ethanolic extract may contain alcohol or carboxylic acid, or ether compounds. On the other hand, the acetone extract may contain alcohol or ether or aromatic or aliphatic compounds. The UV-Vis spectrum of the honey pineapple peels extracted in the distilled water, ethanol, and acetone showed a broad absorption signal at UV region (< 300 nm), four absorption signals at UV region (232-368 nm), and four absorption signals at UV region (231-368 nm) with a weak absorption signal at the visible region at 559 nm, respectively. The distilled water and acetone extracts gave fluorescence signals, however, the ethanolic extract showed no fluorescence intensity. From the FTIR, UV-Vis, and fluorescence spectra characterization, the extracted natural pigments from the honey pineapple peels in distilled water, ethanol, and acetone solvents were identified. The distilled water extract may contain polar flavonoid or steroid compounds while the ethanolic extract may contain polar carotenoid pigments. On the other hand, the acetone extract may contain carotenoid and chlorophyll pigments as shown by an emission signal at 670 nm.

scholarly journals STUDIES IN RODENT POLIOMYELITIS

1942 ◽  
Vol 75 (6) ◽  
pp. 611-629 ◽  
Author(s):  
Claus W. Jungeblut ◽  
Murray Sanders ◽  
Rose R. Feiner
Keyword(s):  
Standard Dose ◽  
Horse Serum ◽  
Age Groups ◽  
The Other ◽  
Mouse Serum ◽  
Intracerebral Injection ◽  
Theiler's Virus ◽  
Poliomyelitis Virus ◽  
Other Hand ◽  
Young Mice

1. SK murine virus maintained over more than 200 serial mouse passages increased in virulence for mice from an initial intracerebral titer of about 1:1 million to a maximum titer of not less than 1:1 billion dilution activity. 2. Following intracerebral injection with murine virus of remote mouse passages, 5 of 13 rhesus monkeys developed a characteristic encephalitic syndrome. Repeated intravenous injection of massive doses of virus caused localized flaccid paralysis in 2 of 14 monkeys. 3. Intracerebral injection of graded doses of murine virus into mice of different age groups caused fatal paralysis in young and old animals alike. Infection with small doses of virus by peripheral routes, while uniformly fatal to young mice, was followed by survival of almost half of the old mice. 4. The incubation period of the disease in young mice infected intracerebrally with a standard dose of murine virus, when studied throughout the period of 1 year, was found considerably lengthened during the summer months. 5. Cross neutralization tests furnished no evidence for any serological relationship between SK murine virus and lymphocytic choriomeningitis virus. Theiler's virus was found to be neutralizable by antimurine horse serum and, to a lesser extent, by concentrated antipoliomyelitis horse serum; however, such inactivation, in both cases, was distinctly inferior to that occurring with SK murine virus. On the other hand, no neutralization whatsoever was obtained between SK murine virus and normal adult mouse serum, whereas the same serum completely neutralized Theiler's virus. Mice surviving infection with Theiler's virus, though acquiring immunity to this virus, remained fully susceptible to reinfection with SK murine virus. 6. Neutralization tests with SK murine virus against poliomyelitis-convalescent monkey sera gave irregular results, but neutralization of murine virus occurred regularly with a hyperimmune antipoliomyelitis horse serum. Hyperimmune antimurine horse and rabbit sera, on the other hand, failed to inactivate three strains of monkey poliomyelitis virus (SK, RMV, Aycock) by intracerebral tests in monkeys. The same sera inactivated murine virus in mice by intraperitoneal, but not by intracerebral injection of virus-serum mixtures. 7. The identity of SK murine virus and its relation to other rodent strains of poliomyelitis virus is discussed on the basis of the available data.

scholarly journals Variation in Chemotherapeutic Susceptibility Associated with Change in Virulence of a Strain of Trypanosoma brucei

1935 ◽  
Vol 35 (2) ◽  
pp. 180-184 ◽  
Author(s):  
C. H. Browning ◽  
R. Gulbransen
Keyword(s):  
Trypanosoma Brucei ◽  
Effective Resistance ◽  
The Other ◽  
Other Hand ◽  
Chemotherapeutic Response ◽  
Curative Action ◽  
Trypanocidal Drugs

A strain of Trypanosoma brucei when recently introduced into mice and imperfectly accommodated to this species of host produced infections relatively resistant to various trypanocidal drugs. On the other hand, when the strain had become highly accommodated and its pathogenicity increased to a maximum as the result of repeated passages, infected animals were readily cured. The exact mechanism on which this difference in resistance may depend has not been investigated. Attention is directed, however, to the important fact that the drugs acted poorly when the strain was in an attenuated state, as shown by the prolonged course of infection and the occurrence of marked fluctuations in the number of parasites present in the blood. Thus the chemotherapeutic response was weak at the time when the host itself was able to exercise an effective resistance. Later, when the host's resistance had become negligible, the curative action of the drugs was pronounced.

scholarly journals A COMPARISON OF THE EFFECTS OF X-RAY AND DRY HEAT ON ANTIBODY FORMATION

1925 ◽  
Vol 41 (2) ◽  
pp. 245-255 ◽  
Author(s):  
James B. Murphy ◽  
Ernest Sturm
Keyword(s):  
Bone Marrow ◽  
Lymphoid Tissue ◽  
Antibody Formation ◽  
Lymphoid Organs ◽  
The Other ◽  
X Ray ◽  
Dry Heat ◽  
Other Hand ◽  
Protective Antibodies

Rabbits x-rayed in doses sufficient to reduce the amount of their lymphoid tissue without damage to the bone marrow showed a definite deficiency in the production of precipitins, bacterial agglutinins, and protective antibodies. On the other hand, rabbits subjected to exposures of dry heat sufficient to increase the activity of the lymphoid organs, on immunization develop antibodies in larger quantity than do untreated animals immunized by the same process.

Immunity in trypanosomiasis

Parasitology ◽  
1964 ◽  
Vol 54 (3) ◽  
pp. 585-591 ◽  
Author(s):  
M. A. Soltys
Keyword(s):  
The Other ◽  
Practical Application ◽  
Freezing And Thawing ◽  
Negative Results ◽  
Other Hand ◽  
Protective Antibodies ◽  
Freund’S Adjuvant ◽  
Freund's Adjuvant ◽  
Positive Results ◽  
Better Than

Success in immunizing rabbits, rats and sheep with dead trypanosomes depends on the method of killing trypanosomes, the frequency of inoculations, and the strain used for immunization. Positive results were obtained when trypanosomes were killed by formalin or by freezing and thawing five times. Negative results were obtained when trypanosomes were killed by 56° C. for half an hour.Animals which received 10 doses of the mixture produced better results than those which received the same amount but only in 2 doses. An addition of Freund's adjuvant improved results, but 10 doses without an adjuvant were still better than 2 doses with an adjuvant. When trypanosomes were killed by 0·5% formalin, only an M variant of T. brucei, which was maintained by frequent passage in mice and was an antibody-sensitive variant, was able to produce in animals protective antibodies and resistance to a homologous ‘M’ variant, but not to an ‘R’ variant. On the other hand, if an ‘R’ variant of the same strain, which was passaged forty-eight times through rabbits and became resistant to antibodies, was killed by 0·5 % formalin and inoculated into animals, no antibodies and resistance could be demonstrated to a homologous ‘R’ variant and an ‘M’ variant.Although it is possible to produce immunity in animals with dead trypanosomes further studies, particularly in the field, are necessary to find its practical application.

Intravenous Diazepam in Status Epilepticus: A Correction

PEDIATRICS ◽  
1973 ◽  
Vol 51 (5) ◽  
pp. 959-959
Author(s):  
James R. West
Keyword(s):  
Status Epilepticus ◽  
Intravenous Administration ◽  
Package Insert ◽  
The Other ◽  
Distilled Water ◽  
Physical Change ◽  
Other Hand ◽  
Intravenous Diazepam

A question has arisen over the recommendation made by Dr. Sidney Carter1 that diazepam be diluted 10 mg in 8 ml distilled water prior to its intravenous administration in status epilepticus. We have noticed that the resulting solution appears turbid, and have had second thoughts about giving such a solution. The package insert for Valium suggests that it not be mixed with other solutions. On the other hand, if it is injected into an aqueous intravascular medium it would seem likely that the same physical change is occurring there.

Social Mobility among Businessmen in a Taiwanese Town

1962 ◽  
Vol 21 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Wolfram Eberhard
Keyword(s):  
Case Studies ◽  
Social Mobility ◽  
Comparative Studies ◽  
Census Data ◽  
The Other ◽  
Detailed Data ◽  
Other Hand

One of the weaknesses of comparative studies of the development of an entrepreneurial class is the lack of detailed data. Often such studies make use of census data which indicate the growth of this class in general but fail to show clearly the origins of new entrepreneurs. Available case studies, on the other hand, do not cover enough ground thus precluding generalizations.

Sign in / Sign up

Export Citation Format

Share Document